Abstract:
:Thermodegradation of labile compounds in the hot injection port of a gas chromatograph causes many problems and can lead to ambiguous, poorly reproducible results. Splitless injection used in trace analysis in order to achieve a lower detection limit may even enhance the difficulties due to the longer residence time of the volatized sample in the injection port. Different techniques were tested to avoid thermodegradation of an epoxide and its corresponding chlorohydrin in a drug substance: variation of the injector temperature, high inlet flow rate during injection by electronic pressure programming, and cool on-column injection. The results showed that on-column injection was superior to splitless injection. The chlorohydrin formed epoxide by release of HCl upon splitless injection. A more detailed investigation of the factors that affect epoxide formation indicates that active sites within the injector are responsible for the degradation and that the reaction is temperature dependent. However, results equivalent to those obtained by on-column injection were obtained for splitless injection when inert materials were used in the injector.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Klick Sdoi
10.1016/0731-7085(95)01307-7subject
Has Abstractpub_date
1995-04-01 00:00:00pages
563-6issue
4-5eissn
0731-7085issn
1873-264Xpii
0731-7085(95)01307-7journal_volume
13pub_type
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2018.03.058
更新日期:2018-06-05 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2006.11.002
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2002-07-31 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/0731-7085(83)80065-x
更新日期:1983-01-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/s0731-7085(98)00259-3
更新日期:1999-05-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2019.01.040
更新日期:2019-03-20 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2006.12.022
更新日期:2007-06-28 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2006.04.012
更新日期:2006-09-26 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章,评审
doi:10.1016/j.jpba.2010.11.030
更新日期:2011-06-25 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2016.12.014
更新日期:2017-02-20 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2008.04.026
更新日期:2008-09-10 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2016.07.045
更新日期:2016-09-10 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2004.09.024
更新日期:2005-04-29 00:00:00
abstract::Cyclodextrins are cyclic oligosaccharides able to form inclusion complexes with a variety of hydrophobic guest molecules, positively modifying their physicochemical properties. A thorough analytical characterization of cyclodextrin complexes is of fundamental importance to provide an adequate support in selection of t...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章,评审
doi:10.1016/j.jpba.2015.01.058
更新日期:2015-09-10 00:00:00
abstract::An extracellular thermostable antibacterial peptide designated as MFAP9 was purified from marine Aspergillus fumigatus BTMF9 by ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE-sepharose column. The molecular weight of MFAP9 was found to be∼3 kDa in SDS-PAGE gel corresponding a single i...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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更新日期:1988-01-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2007.04.029
更新日期:2007-09-03 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/0731-7085(95)01647-3
更新日期:1996-02-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2008.01.048
更新日期:2008-04-14 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2004.12.007
更新日期:2005-06-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:2003-08-08 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
abstract::A simple and rapid quantitative analytical method for the simultaneous detection of celecoxib and its two main metabolites, hydroxycelecoxib (celecoxib-OH) and celecoxib carboxylic acid (celecoxib-COOH), in rat plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The plasma sample was ...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
doi:10.1016/j.jpba.2014.12.004
更新日期:2015-03-25 00:00:00
abstract::A simple, selective and sensitive method has been developed to determine debrisoquine and 4-hydroxydebrisoquine in human urine. Separation of the analytes was obtained using a mobile phase of 0.1 M sodium dihydrogen phosphate-acetonitrile (87:13, v/v) and a muBondapak C18 column. The column effluent was monitored with...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00