Abstract:
:The chromatographic behaviour (retention, selectivity, peak shape and resolution) of seven tricyclic antidepressants (TCAs), amitryptiline, clomipramine, doxepin, imipramine, maprotiline, nortryptiline and trimipramine, was examined. Conventional unendcapped Cs and C18 columns and an endcapped XTerra MS C18 column recommended for the analysis of basic compounds were used together with acetonitrile-water and micellar sodium dodecylsulfate (SDS)-pentanol mobile phases. The two best combinations were XTerra C18/acetonitrile, which yielded the largest efficiencies and resolution, and C8/SDS-pentanol, which eliminated the peak tails that were still observed with the XTerra C18 column. Both the systems were used to develop simple chromatographic procedures for the control of TCAs in pharmaceutical formulations using UV detection. The selected mobile phase compositions were 35% (v/v) acetonitrile (XTerra C18 column) and 0.075 M SDS-6% (v/v) pentanol (C8 column), both at pH 3. Satisfactory recoveries were achieved in both cases, with intra- and inter-day relative standard deviations (RSDs) always below 0.6 and 2.0%, respectively. The preparation of the samples was simple in both modes, since a previous extraction of the drugs was not needed. The micellar mode has, however, the advantage of using a smaller amount of organic solvent, which is retained in the micellar SDS solution. The C8 column is also less expensive.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Ruiz-Angel MJ,Carda-Broch S,Simó-Alfonso EF,García-Alvarez-Coque MCdoi
10.1016/s0731-7085(03)00048-7subject
Has Abstractpub_date
2003-04-24 00:00:00pages
71-84issue
1eissn
0731-7085issn
1873-264Xpii
S0731-7085(03)00048-7journal_volume
32pub_type
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