A study of low-molecular-weight organic acid urinary profiles in prostate cancer by a new liquid chromatography-tandem mass spectrometry method.

Abstract:

:Metabolomic studies constantly require high throughput screenings, and this drives development and optimization of methods that include more analytes in a single run, shorten the analysis time and simplify sample preparation. The aim of the study was to develop a new simple and fast liquid chromatography-tandem mass spectrometry-based methodology for quantitative analysis of a panel of ten organic acids in urine. The metabolites selected for the study include ten molecules potentially associated with cancer development. Chromatographic separation involved a Phenomenex Synergi Hydro-RP column under gradient conditions. Quantitation of the analytes was performed in multiple reaction monitoring mode under negative ionization. Validation parameters were satisfactory and in line with the international guidelines. The methodology enabled us to analyze urine samples collected from prostate cancer (PC) (n = 49) and benign prostate hyperplasia (BPH) (n = 49) patients. The obtained concentrations were normalized with urinary specific gravity (USG) prior to statistical analysis. Five analytes were quantified in all urine samples and we observed the following USG-normalized concentration ranges: citric acid (146.5-6339.8), 3-hydroxyisobutyric acid (22.5-431.7), 2-ketoglutaric acid (4.4-334.4), lactic acid (10.1-786.3), succinic acid (4.1-500.5). 3-hydroxyisobutyric acid significantly decreased between two groups of prostate cancer patients: ≥7 Gleason patients and <7 Gleason patients. Quick sample preparation limited to "dilute and shoot" makes the developed methodology a great tool for future metabolomic studies, especially for detecting disturbances in energy metabolism (Krebs cycle) and amino acids metabolism. The research also broadens our knowledge on the alteration of selected organic acids in PC and BPH patients.

journal_name

J Pharm Biomed Anal

authors

Klupczynska A,Plewa S,Sytek N,Sawicki W,Dereziński P,Matysiak J,Kokot ZJ

doi

10.1016/j.jpba.2018.06.059

subject

Has Abstract

pub_date

2018-09-10 00:00:00

pages

229-236

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(18)30336-4

journal_volume

159

pub_type

杂志文章
  • Electrochemical study of nisoldipine: analytical application in pharmaceutical forms and photodegradation.

    abstract::The anodic and cathodic behavior of nisoldipine, 3-isobutyl-5-methyl-1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)pyridine-3 ,5-dicarboxylate, are reported. This drug belongs to the nitroaryl-1,4-dihydropyridine family, known as calcium channel antagonist and employed in therapeuticalls as peripheral and cerebral vasodil...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(97)00122-2

    authors: Alvarez-Lueje A,Naranjo L,Núñez-Vergara LJ,Squella JA

    更新日期:1998-01-01 00:00:00

  • The isolation of Nepsilon-formyl-L-lysine from the reaction between formaldehyde and L-lysine and its identification by OPLC and NMR spectroscopy.

    abstract::From the reaction between l-lysine and formaldehyde, Nepsilon-formyl-L-lysine was isolated by means of ion-exchange column chromatography. The identification of Nepsilon-formyl-L-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/0731-7085(85)80042-x

    authors: Tyihák E,Trézl L,Kolonits P

    更新日期:1985-01-01 00:00:00

  • An overview of in vitro dissolution/release methods for novel mucosal drug delivery systems.

    abstract::In vitro dissolution/release tests are an important tool in the drug product development phase as well as in its quality control and the regulatory approval process. Mucosal drug delivery systems are aimed to provide both local and systemic drug action via mucosal surfaces of the body and exhibit significant differenc...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/j.jpba.2017.06.072

    authors: Jug M,Hafner A,Lovrić J,Kregar ML,Pepić I,Vanić Ž,Cetina-Čižmek B,Filipović-Grčić J

    更新日期:2018-01-05 00:00:00

  • Characterization of a new illicit phosphodiesterase-type-5 inhibitor identified in the softgel shell of a dietary supplement.

    abstract::A new sildenafil analog has been identified in the softgel shell of a dietary supplement. The compound was investigated by UV spectroscopy and high-resolution MS analysis, leading to the proposed structure 1-methyl-5-{5-[2-(4-methylpiperazin-1-yl)acetyl]-2-propoxyphenyl}-3-propyl-1,6-dihydro-7H-pyrazolo[4,3-d]pyrimidi...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.08.031

    authors: Doi T,Takahashi K,Yamazaki M,Asada A,Takeda A,Kiyota K,Tagami T,Sawabe Y,Yamano T

    更新日期:2018-11-30 00:00:00

  • Validation of a dissolution method with HPLC analysis for lasofoxifene tartrate low dose tablets.

    abstract::A dissolution method with high performance liquid chromatography (HPLC) analysis was validated for an immediate release low dose tablet formulation. The method was validated to meet requirements for a global regulatory filing and this validation included specificity, precision, linearity, accuracy and range. Validatio...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2007.04.032

    authors: Space JS,Opio AM,Nickerson B,Jiang H,Dumont M,Berry M

    更新日期:2007-09-03 00:00:00

  • Fluorescence determination of sulphobutylether-beta-cyclodextrin in human plasma by size exclusion chromatography with inclusion complex formation.

    abstract::A selective method for the determination of sulphobutylether-beta-cyclodextrin (SBECD) in human plasma has been developed and validated over the range 4-200 microg ml(-1). SBECD is extracted from plasma using end-capped cyclohexyl solid phase extraction cartridges. This is followed by high performance size exclusion c...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(99)00283-6

    authors: Gage R,Venn RF,Bayliss MA,Edgington AM,Roffey SJ,Sorrell B

    更新日期:2000-06-01 00:00:00

  • Capillary zone electrophoresis in pharmaceutical and biomedical analysis.

    abstract::Capillary zone electrophoresis is a technique that is rapidly gaining popularity in the pharmaceutical industry because of the wide range of compounds applicable to the technique. These range from small biologically active molecules through to large nucleotides and proteins. This review outlines the historical develop...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/0731-7085(93)e0012-c

    authors: Smith NW,Evans MB

    更新日期:1994-05-01 00:00:00

  • Clara cell secretory protein: determination of serum levels by an enzyme immunoassay and its importance as an indicator of bronchial asthma in children.

    abstract::Clara cell secretory protein (CC16) is a 16kDa protein secreted by Clara cells in the lining fluid of bronchiolar and bronchial epithelium. CC16 presents several biologic properties, and has been shown to have immunomodulatory and anti-inflammatory activity. It may play a role in controlling inflammation in the airway...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/S0731-7085(03)00570-3

    authors: Gioldassi XM,Papadimitriou H,Mikraki V,Karamanos NK

    更新日期:2004-03-01 00:00:00

  • Live cell extraction and HPLC-MS analysis for predicting bioactive components of traditional Chinese medicines.

    abstract::A novel strategy for predicting bioactive components in traditional Chinese medicines (TCM) using live cell extraction and high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS) analysis was proposed. The hypothesis is that when cells are incubated together with the extract of TCM...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2006.01.014

    authors: Li SL,Li P,Sheng LH,Li RY,Qi LW,Zhang LY

    更新日期:2006-05-03 00:00:00

  • Simultaneous quantitative analysis of polyethylene glycol (PEG), PEGylated paclitaxel and paclitaxel in rats by MS/MSALL technique with hybrid quadrupole time-of-flight mass spectrometry.

    abstract::PEGylation is practically one of most important modifications of drugs including small molecules, peptides and proteins, which has been proven to dramatically improve physicochemical properties and pharmacokinetic behavior of the PEGylated drugs. However, it is a challenge currently to quantitatively analyze PEG and P...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2017.06.053

    authors: Sun H,Zhang Q,Zhang Z,Tong J,Chu D,Gu J

    更新日期:2017-10-25 00:00:00

  • Structure elucidation of a process-related impurity of dapoxetine.

    abstract::Unknown by-product associated with the synthesis of dapoxetine was isolated. The structure elucidation of this new compound using accurate mass data and NMR spectroscopy is presented herein. The unambiguous resonance assignment concluded to the formation of a tricyclic compound 4-phenyl-2H,3H,4H-naphtho[1,2-b]pyran, a...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2014.04.002

    authors: Darcsi A,Tóth G,Kökösi J,Béni S

    更新日期:2014-08-05 00:00:00

  • Reversed phase HPLC of Met58 oxidized rhIL-11: oxidation enhanced by plastic tubes.

    abstract::The hydrophobicity of human recombinant interleukin 11 (rhIL-11) with an oxidized Met58 residue is nearly identical to the hydrophobicity of native rhIL-11. Consequently, separation of these species using standard gradient elution or isocratic elution is very difficult. Using an optimized, shallow gradient RP-HPLC met...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(00)00419-2

    authors: Yokota H,Saito H,Masuoka K,Kaniwa H,Shibanuma T

    更新日期:2000-12-15 00:00:00

  • Pharmaceutical impurities and degradation products: uses and applications of NMR techniques.

    abstract::Current standards and regulations demand the pharmaceutical industry not only to produce highly pure drug substances, but to achieve a thorough understanding of the impurities accompanying their manufactured drug substances and products. These challenges have become important goals of process chemistry and have steadi...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/j.jpba.2014.04.016

    authors: Maggio RM,Calvo NL,Vignaduzzo SE,Kaufman TS

    更新日期:2014-12-01 00:00:00

  • Liquid chromatographic determination of amoxycillin and clavulanic acid in pharmaceutical preparations.

    abstract::A liquid chromatographic method for the simultaneous determination of amoxycillin and potassium clavulanate in tablet and suspension preparations is presented. The method specifies reversed phase column and a buffered mobile phase (CH3OH + KH2PO4-buffer pH 6 + H2O, 15:1:84) isocratically at a rate of 1.0 ml min-1, wit...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/0731-7085(91)80214-t

    authors: Abounassif MA,Abdel-Moety EM,Mohamed ME,Gad-Kariem RA

    更新日期:1991-01-01 00:00:00

  • A direct LC/MS/MS method for the determination of ciclopirox penetration across human nail plate in in vitro penetration studies.

    abstract::Due to severe chelating effect caused by N-hydroxylpyridone group of ciclopirox, there is no published direct HPLC or LC/MS/MS method for the determination of ciclopirox in any in vitro or in vivo matrix. Instead, the time-consuming pre-column derivatization methods have been adapted for indirect analysis of ciclopiro...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2009.08.019

    authors: Bu W,Fan X,Sexton H,Heyman I

    更新日期:2010-01-05 00:00:00

  • Chromatographic methods and sample pretreatment techniques for aldehydes determination in biological, food, and environmental samples.

    abstract::Aldehydes are very reactive carbonyl compounds that are widespread naturally. Aldehydes can be produced in vivo by oxidative reactions and are able to disturb biological functions through binding and modifying biomolecules. Thus, it is necessary to determine their levels in biological samples to estimate their possibl...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审

    doi:10.1016/j.jpba.2019.112782

    authors: Kishikawa N,El-Maghrabey MH,Kuroda N

    更新日期:2019-10-25 00:00:00

  • Method optimization of hydrophilic interaction chromatography separation of nucleotides using design of experiment approaches I: Comparison of several zwitterionic columns.

    abstract::A systematic method in hydrophilic interaction chromatography (HILIC) was developed for the separation of four monophosphate nucleotides using design of experiment (DOE) approaches. Three HPLC parameters, the buffer concentration (ammonium acetate concentration), gradient time, and temperature, were evaluated within t...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.05.014

    authors: Arase S,Kimura S,Ikegami T

    更新日期:2018-09-05 00:00:00

  • Application of HPLC and HPTLC for the simultaneous determination of tizanidine and rofecoxib in pharmaceutical dosage form.

    abstract::Two methods are described for the simultaneous determination of tizanidine and rofecoxib in binary mixture. The first method was based on HPTLC separation of the two drugs followed by densitometric measurements of their spots at 311 nm. The separation was carried out on Merck HPTLC aluminium sheets of silica gel 60 F2...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2004.09.036

    authors: Kaul N,Dhaneshwar SR,Agrawal H,Kakad A,Patil B

    更新日期:2005-02-07 00:00:00

  • Development and qualification of a high sensitivity, high throughput Q-PCR assay for quantitation of residual host cell DNA in purification process intermediate and drug substance samples.

    abstract::Methods of high sensitivity, accuracy and throughput are needed for quantitation of low level residual host cell DNA in purification process intermediates and drug substances of therapeutic proteins. In this study, we designed primer/probe sets targeting repetitive Alu repeats or Alu-equivalent sequences in the human,...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2014.07.037

    authors: Zhang W,Wu M,Menesale E,Lu T,Magliola A,Bergelson S

    更新日期:2014-11-01 00:00:00

  • Microwave-assisted extraction for Hibiscus sabdariffa bioactive compounds.

    abstract::H. sabdariffa has demonstrated positive results against chronic diseases due to the presence of phytochemicals, mainly phenolic compounds. The extraction process of bioactive compounds increases the efficient collection of extracts with high bioactivity. Microwave-Assisted Extraction (MAE) constituted a "green technol...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2018.04.050

    authors: Pimentel-Moral S,Borrás-Linares I,Lozano-Sánchez J,Arráez-Román D,Martínez-Férez A,Segura-Carretero A

    更新日期:2018-07-15 00:00:00

  • Investigation of N-terminal glutamate cyclization of recombinant monoclonal antibody in formulation development.

    abstract::The N-terminal glutamic acid (Glu) can be cyclized to form pyroglutamate (pGlu). Recent studies have suggested that N-terminal pGlu formation is an important posttranslational or co-translational event and is greatly facilitated by the enzyme glutaminyl cyclase, although the impact of the N-terminal cyclization on the...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2006.05.008

    authors: Yu L,Vizel A,Huff MB,Young M,Remmele RL Jr,He B

    更新日期:2006-10-11 00:00:00

  • Pharmacokinetics, tissue distribution, and safety evaluation of a ligustilide derivative (LIGc).

    abstract::Ligustilide (LIG) is the main active ingredient of Angelica sinensis (Oliv.) Diels and has a neuroprotective effect against ischemic stroke. However, owing to its multi-conjugated unstable structure, the compound has poor drug-forming properties. Therefore, we synthesized highly stable colorless needle crystals (known...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2020.113140

    authors: Zhang Y,Zhang Y,Han Y,Tian Y,Wu P,Xin A,Wei X,Shi Y,Zhang Z,Su G,Shi Y,Liu J

    更新日期:2020-04-15 00:00:00

  • Optical biosensor analysis in studying herpes simplex virus glycoprotein D binding to target nectin1 receptor.

    abstract::Studies on molecular interactions between cellular receptors of herpes simplex virus (HSV) and the viral glycoproteins showing receptor-binding activity are of great relevance for understanding the molecular basis of virus entry. Information on such interactions further provides the basis for a rational design of anti...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(03)00177-8

    authors: Bertucci C,Cimitan S,Menotti L

    更新日期:2003-08-08 00:00:00

  • Correlation between structure, retention, property, and activity of biologically relevant 1,7-bis(aminoalkyl)diazachrysene derivatives.

    abstract::The physicochemical properties, retention parameters (R(M)(0)), partition coefficients (logP(OW)), and pK(a) values for a series of thirteen 1,7-bis(aminoalkyl) diazachrysene (1,7-DAAC) derivatives were determined in order to reveal the characteristics responsible for their biological behavior. The investigated compou...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2012.08.025

    authors: Šegan S,Trifković J,Verbić T,Opsenica D,Zlatović M,Burnett J,Šolaja B,Milojković-Opsenica D

    更新日期:2013-01-01 00:00:00

  • Plasmatic dermatan sulfate and chondroitin sulfate determination in mucopolysaccharidoses.

    abstract::The evaluation of plasmatic galactosaminoglycans, dermatan sulfate (DS) and chondroitin sulfate (CS) can be helpful in the early identification of MPS patients, also considering that primary storage of one type of GAG can lead to secondary accumulation of other lysosomal substrates. We explore the possibility to deter...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2013.06.026

    authors: Volpi N,Maccari F,Galeotti F,Zampini L,Santoro L,Padella L,Galeazzi T,Gabrielli O,Coppa GV

    更新日期:2013-11-01 00:00:00

  • Intact charge variant analysis of ziv-aflibercept by cationic exchange liquid chromatography as a proof of concept: Comparison between volatile and non-volatile salts in the mobile phase.

    abstract::Ziv-aflibercept (ziv-AFL) is a complex fusion protein which is widely used in hospitals for the treatment of colorectal metastatic cancer. Charge variants are critical attributes for assessing post-transitional modifications (PMTs) that have to be controlled during the development and manufacture of these proteins and...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2020.113233

    authors: Pérez-Robles R,Cuadros-Rodríguez L,Salmerón-García A,Cabeza-Barrera J,Navas N

    更新日期:2020-06-05 00:00:00

  • Determination of selenium in dietary supplements by optical emission spectrometry after alkaline dissolution and subsequent headspace solid phase microextraction.

    abstract::Headspace solid phase microextraction (HSSPME) of chemically generated selenium hydride from alkaline solution followed by thermal desorption (TD) coupled directly to a microwave plasma (MWP) source has been examined for the optical emission spectrometric (OES) determination of Se. Various chemical and operating param...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2012.11.011

    authors: Tyburska A,Jankowski K

    更新日期:2013-02-23 00:00:00

  • Molecularly imprinted polymer for caffeic acid by precipitation polymerization and its application to extraction of caffeic acid and chlorogenic acid from Eucommia ulmodies leaves.

    abstract::Molecularly imprinted polymers (MIPs) for caffeic acid (CA) were prepared using 4-vinylpyridine and methacrylamide (MAM) as functional monomers, divinylbenzene as a crosslinker and acetonitrile-toluene (3:1, v/v) as a porogen by precipitation polymerization. The use of MAM as the co-monomer resulted in the formation o...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2015.12.052

    authors: Miura C,Matsunaga H,Haginaka J

    更新日期:2016-08-05 00:00:00

  • Keratin immobilized on silica as a new stationary phase for chromatographic modelling of skin permeation.

    abstract::Skin permeability of organic compounds depends on their lipophilicity but can also be affected by compounds interactions with specific skin components. A good chromatographic model of percutaneous penetration determined solely by lipophilicity is provided by the immobilized artificial membrane (IAM) columns. To comple...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/s0731-7085(96)02009-2

    authors: Turowski M,Kaliszan R

    更新日期:1997-06-01 00:00:00

  • Revisiting thin-layer chromatography as a lipophilicity determination tool--a comparative study on several techniques with a model solute set.

    abstract::The lipophilicity of a compound is a fundamental property related to pharmaceutical and biomedical activity. As many approaches are mixed together in every-day published studies, the subject needs some standardization. The paper presents a comparative study on several approaches of TLC lipophilicity determination: a s...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章

    doi:10.1016/j.jpba.2010.06.024

    authors: Komsta Ł,Skibiński R,Berecka A,Gumieniczek A,Radkiewicz B,Radoń M

    更新日期:2010-12-01 00:00:00