Abstract：
:A specific and reliable liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed for the simultaneous determination of tetrahydropalmatine (THP), tetrahydroberineper (THB), tetrahydrocoptisine (THC) and corydaline (CDL) in rat plasma using jatrorrhizine as internal standard. Plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Zorbax Eclipse Plus C18 column (3.0 mm × 100 mm, 1.8 μm) with gradient elution using acetonitrile-0.1% formic acid water as mobile phase at a flow rate of 0.3 mL/min. The detection was carried out by a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. The mass transition ion-pair was followed as m/z 356.2→192.1, m/z 324.1→176.0, m/z 340.1→176.0, and m/z 370.3→192.1 for THP, THC, THB and CDL. Linear calibration curves were obtained over the range of 0.2-2000 ng/mL, and the method limits were 2.0 ng/mL for THC, 0.2 ng/mL for THB, 1.0 ng/mL for THP, and 0.4 ng/mL for CDL, respectively. The mean recovery of the analytes ranged from 85.51% to 93.99%. The intra- and inter-day precisions were in the range of 1.21-4.73% and the accuracies were between 92.23% and 98.23%. The validated method was successfully applied to a pharmacokinetic study of THP, THB, THC and CDL in rat plasma following oral administration of Jitai tablet.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Wang S,Liu L,Wang L,Jiang P,Xiang L,Zhang W,Liu Rdoi
10.1016/j.jpba.2012.09.001subject
Has Abstractpub_date
2013-01-01 00:00:00pages
80-8eissn
0731-7085issn
1873-264Xpii
S0731-7085(12)00490-6journal_volume
72pub_type
杂志文章abstract：:Pogostemon cablin, originating in Malaysia and India, is cultivated in southern China including Guangdong and Hainan Province, which was called GuangHuoXiang to differentiate it from the HuoXiang of the north, the species Agastache rugosa, that it resembles. Essential oil of P. cablin mainly contributes to the pharmac...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2005.09.015
更新日期：2006-09-18 00:00:00
abstract：:A reversed phase high-performance liquid chromatographic method with detection at 220 nm was developed and validated for the determination of piperacillin, I, and tazobactam, II, in Tazocin injectable powder. Acetaminophen was used as internal standard. A Hypersil BDS RP-C(18) column (250 x 4.6 mm), 5 microm particle ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(02)00129-2
更新日期：2002-07-31 00:00:00
abstract：:The tridecapeptide afamelanotide (Scenesse®) is a congener of α-melanocyte stimulating hormone (α-MSH). Upon binding to the melanocortin 1 receptor (MC1R) on the surface of pigment cells of the skin, the melanocytes, α-MSH or afamelanotide trigger the synthesis of cAMP, which stimulates the synthesis of melanin and th...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2012.11.040
更新日期：2013-03-05 00:00:00
abstract：:Di(2-ethylhexyl)phthalate (DEHP), a plasticiser present in infusion equipment, is known to be harmful to human health. Various studies have shown that DEHP is released into drug solutions from polyvinyl chloride (PVC) infusion lines. New multi-layer tubing has therefore been marketed to overcome this problem. We asses...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2003.12.018
更新日期：2004-04-01 00:00:00
abstract：:Capillary electrophoresis (CE) was applied to the study of several inhibitors of the angiotensin-converting enzyme. Separation of the compounds was performed by means of two phosphate buffers (each 100 mM) at pH 7.0 and 6.25, respectively [S. Hillaert, W. Van den Bossche, J. Chromatogr. A, 895 (2000) 33-42.]. Due to t...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(01)00366-1
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,随机对照试验
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更新日期：2017-04-15 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2014.07.037
更新日期：2014-11-01 00:00:00
abstract：:Thiazolidinediones are known for their activity against Type 2 diabetes and are currently being repurposed for their potent anti-cancer activity. In the present study, we have assessed in vitro metabolic properties and in vivo pharmacokinetic parameters of a novel thiazolidinedione derivative, BIT-15-67, a potential a...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(01)00471-x
更新日期：2001-12-01 00:00:00
abstract：:Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100 degrees C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2008.07.017
更新日期：2008-11-04 00:00:00
abstract：:Efonidipine hydrochloride is a new generation dihydropyridine Ca(2+) channel blocker designed to inhibit both T-type and L-type Ca(2+) channels. Efonidipine possesses a chiral carbon and is clinically administered as a racemate. In the present study, an enantioselective and sensitive LC-MS/MS method of determining efo...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2016.01.039
更新日期：2016-04-15 00:00:00
abstract：:A simple, specific and reliable 1H-NMR spectroscopic method for the quantitative determination of the optical purity of indacrinone is described. After conversion of the S(+)- and R(-)-enantiomers to their methyl ester derivatives, they were coordinated with chiral europium(III) or praseodymium(III) shift reagents in ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(89)80016-0
更新日期：1989-01-01 00:00:00
abstract：:Zishen Yutai Pills (ZYP) is a well-known Chinese patent medicine which has been used to prevent recurrent miscarriage and treat threatened abortion in China. In this study, a comprehensive strategy combining ultrahigh performance liquid chromatography coupled with charged aerosol detector (UHPLC-CAD) fingerprint and m...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2020.113570
更新日期：2020-11-30 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2014.03.005
更新日期：2014-08-05 00:00:00
abstract：:A reverse phase high performance liquid chromatographic method to determine tizanidine (TZ) and rofecoxib (RF) in combination is proposed and applied to the pharmaceuticals. This method allows the determination of 0.1-0.5 microg/ml of TZ and 1.2-6.0 microg/ml of RF along with 10 microg/ml of nimesulide (internal stand...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2004.09.039
更新日期：2005-02-07 00:00:00
abstract：:The evaluation of plasmatic galactosaminoglycans, dermatan sulfate (DS) and chondroitin sulfate (CS) can be helpful in the early identification of MPS patients, also considering that primary storage of one type of GAG can lead to secondary accumulation of other lysosomal substrates. We explore the possibility to deter...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2013.06.026
更新日期：2013-11-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2004.08.009
更新日期：2004-11-19 00:00:00
abstract：:The methods for analysis of platinum-based anti-cancer drugs in biological media are reviewed in this paper. Although emphasis is placed on cisplatin, attention is also given to several of its analogues (carboplatin, iproplatin) and their degradation and biotransformation products. In an introductory section a short d...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：1998-10-01 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2004.10.046
更新日期：2005-02-23 00:00:00
abstract：:Indole-3-carbinol is a natural glucosinolate known for prevention of human breast, prostate and other types of cancer and it started to be used in commercial preparations, as food supplements. However no analytical method has been proposed for quality control of nutraceuticals with this substance yet. In this paper a ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2015.12.039
更新日期：2016-02-20 00:00:00
abstract：:A method for the analysis of aqueous samples of isosorbide-5-mononitrate (5-ISMN) is presented. It is based on packed column supercritical fluid chromatography (SFC) using 20% of 2-propanol in carbon dioxide as the mobile phase and a diol silica column as the stationary phase. Using the described conditions it is poss...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(02)00072-9
更新日期：2002-06-20 00:00:00
abstract：:Juglone (JL), as one of the major bioactive components present in the bark of Juglans mandshruica Maxim, exhibits versatile bioactivities, especially anti-cancer activity. To better understand the pharmacokinetic properties of juglone, the protein binding rate of juglone was determined by ultrafiltration method, and t...
journal_title：Journal of pharmaceutical and biomedical analysis
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更新日期：2017-07-15 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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更新日期：2014-01-01 00:00:00
abstract：:SU101 or leflunomide, has been studied extensively because of its anti-cancer and immunomodulating properties. The parent isoxazole compound is converted in vitro and metabolized in vivo to an open ring isomeric form, SU0020. Several pharmacological activities have been reported for the parent and metabolite compounds...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：2002-05-15 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：2019-09-10 00:00:00
abstract：:Protandim is an over-the-counter herbal dietary supplement. The key components of Protandim, i.e., epigallocatechin-3-gallate (EGCG), silibinin (SIL), and curcumin (CUR) were simultaneously analyzed through a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method in plasma and different tissues after admini...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：2019-06-05 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
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更新日期：2020-05-30 00:00:00
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,随机对照试验
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更新日期：2017-01-05 00:00:00
abstract：:An NMR-based metabolomics approach was conducted to holisticly explore the effect of XFZYD (a traditional Chinese medicine formula) on high-fat diet induced hyperlipidemia rats with one of the commonly used antihyperlipidemic agents, simvastatin as the positive control. NMR spectra from blood plasma combined with stat...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2013.02.014
更新日期：2013-05-05 00:00:00
