当前位置： SCI文献检索 > JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS期刊下所有文献 > Drug-protein binding mechanism of juglone for early pharmacokinetic profiling: Insights from ultrafiltration, multi-spectroscopic and molecular docking methods.

Drug-protein binding mechanism of juglone for early pharmacokinetic profiling: Insights from ultrafiltration, multi-spectroscopic and molecular docking methods.

Abstract：

:Juglone (JL), as one of the major bioactive components present in the bark of Juglans mandshruica Maxim, exhibits versatile bioactivities, especially anti-cancer activity. To better understand the pharmacokinetic properties of juglone, the protein binding rate of juglone was determined by ultrafiltration method, and the binding affinity and mechanism between JL and human serum albumin (HSA) was investigated in vitro through multi-spectroscopic, thermodynamic, and molecular modeling methods. The binding degree of JL was measured more than 99.0% which suggested that JL had high binding ability to serum albumin. Fluorescence data showed that juglone quench the intrinsic fluorescence of HSA upon forming the JL-HSA nonfluorescent complex at 1:1 stoichiometric proportion, and the complex formation had a high affinity of 104 L·mol-1. Meanwhile, the site marker competitive experiments and the thermodynamic parameters (ΔG=-26.08 kJ·mol-1, ΔH=-16.34 kJ·mol-1, ΔS=32.69 J·mol-1·K-1) indicated that juglone could spontaneously bound to the site I (subdomain IIA) of HAS through hydrophobic and hydrogen bonding interactions. As further revealed by the synchronous fluorescence, three-dimensional fluorescence, Fourier transform infrared (FT-IR) and circular dichroism (CD) spectroscopy, JL could cause conformational and structural alterations of HSA. Additionally, molecular docking was employed to further define the specific binding site and the result was in accordance with the conclusion of experimental analysis. The present work provided reasonable models helping us further understand the pharmacokinetics, pharmacological and toxic effects of JL in vivo and supplied an important insight for the applications of JL in the clinical research.

journal_name

J Pharm Biomed Anal

journal_title

Journal of pharmaceutical and biomedical analysis

authors

Zhao P,Gao G,Zhang L,Cai Q,Lu N,Cheng L,Li S,Hou X

doi

10.1016/j.jpba.2017.03.036

subject

Has Abstract

pub_date

2017-07-15 00:00:00

pages

262-269

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(17)30041-9

journal_volume

141

pub_type

杂志文章

在线工具

Drug-protein binding mechanism of juglone for early pharmacokinetic profiling: Insights from ultrafiltration, multi-spectroscopic and molecular docking methods.