Abstract:
:Growth of the A549 cell line in a perfusion system suitable for use in a magnetic resonance study has been characterized and shown to be stable physiologically and hence appropriate for serial observations. Several methods of monitoring cell growth were compared to assess the behavior of the cells in this system. Comparison between NMR metabolite data and cell growth via cell counting showed that 31P NMR signals accurately reported cell doubling time. In contrast to most NMR cell culture systems, viable cells can be recovered from the perfusion system after the NMR measurements for further biochemical studies. These data further suggest that this system will be useful for studying the physiology and biochemistry of exponentially growing cells for at least two days in NMR tube culture.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Shankland EG,Livesey JC,Wiseman RW,Krohn KAsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
49-58issue
1eissn
0862-8408issn
1802-9973journal_volume
51pub_type
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journal_title:Physiological research
pub_type: 杂志文章
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journal_title:Physiological research
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journal_title:Physiological research
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doi:
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journal_title:Physiological research
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journal_title:Physiological research
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doi:
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doi:
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journal_title:Physiological research
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