Abstract:
:The 25-hydroxyvitamin D(3)-24-hydroxylase mRNA is tightly and reciprocally regulated by 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) and parathyroid hormone (PTH). The upregulation of the 24-hydroxylase by 1,25(OH)(2)D(3) is well established and occurs at the transcriptional level through two vitamin D response elements in the promoter of the gene. However, this induction is blocked by the protein synthesis inhibitor cycloheximide (CHX) indicating a protein component in the regulation pathway. CHX treatment reduced total vitamin D receptor (VDR) protein levels in cells, but reintroduction of VDR and/or retinoid X receptor protein into cells by transfection did not reduce the inhibition by CHX. This indicates that production of another transcription factor or mRNA-stabilizing protein synthesized in response to 1,25(OH)(2)D(3) is required for optimal accumulation of 24-hydroxylase mRNA. PTH downregulates the 24-hydroxylase mRNA by affecting its stability. The half-life of 24-hydroxylase mRNA is reduced 4.2-fold in AOK-B50 cells by PTH. Untranslated regions of the 24-hydroxylase mRNA in reporter gene assays did not confer PTH responsiveness. Further analysis of the coding region of the rat 24-hydroxylase may reveal sites of action of PTH.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Zierold C,Mings JA,DeLuca HFdoi
10.1002/jcb.10341subject
Has Abstractpub_date
2003-02-01 00:00:00pages
234-7issue
2eissn
0730-2312issn
1097-4644journal_volume
88pub_type
杂志文章,评审abstract::Two dimensional (2D) co-cultures of human bone marrow stromal cells (HBMSCs) and human umbilical vein endothelial cells (HUVECs) stimulate osteoblastic differentiation of HBMSCs, induce the formation of self-assembled network and cell interactions between the two cell types involving many vascular molecules. Because o...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24407
更新日期:2013-03-01 00:00:00
abstract::Human eosinophil-derived neurotoxin (EDN, RNase2) and eosinophil cationic protein (ECP, RNase3) sequences possess as high as 92% identity in their promoter regions. The major difference within this region is a 34-nucleotide (34-nt) segment appeared only in the edn promoter. In addition, six discrete segments existed i...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22008
更新日期:2009-02-01 00:00:00
abstract::It is well accepted, that there is communication between osteoblasts and endothelial cells. However, the influence of endothelial cells on the differentiation of bone precursors into mature osteoblasts is not yet well understood. We therefore studied the effect of human umbilical vein endothelial cells (HUVEC) on huma...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20818
更新日期:2006-07-01 00:00:00
abstract::Four detergents have been compared for identification of the Plasmodium knowlesi variant antigen on infected erythrocytes by immunoprecipitation analysis. Erythrocytes infected with late trophozoite and schizont forms of cloned asexual parasites were labeled by lactoperoxidase-catalyzed radioiodination and extracted e...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240240310
更新日期:1984-01-01 00:00:00
abstract::Accumulating studies indicates that circular RNAs (circRNAs) play an imperative role in modulating cancer progression and metastasis. In the previous study, elevated circ_0029426 was first observed in glioblastoma (GBM) tissues compared with normal tissues by circRNA microarray. Our aim is to study the function and me...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28313
更新日期:2019-06-01 00:00:00
abstract::NELL-1 (Nel-like molecule-1) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. NELL-1 protein has been observed to promote bone and cartilage differentiation and to suppress adipogenesis in both in vitro and in vivo models. Despite these findings, the cell surface recepto...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24253
更新日期:2012-12-01 00:00:00
abstract::We report here that the antiestrogen tamoxifen (TAM) induces cell death in human breast cancer cell line MCF-7. We assessed the type of cell death induced by TAM in this breast cancer cell line on the basis of morphological and biochemical characteristics. Dying cells showed morphological characteristics of apoptosis,...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4644(19960401)61:1%3C9::AID-JCB
更新日期:1996-04-01 00:00:00
abstract::The expression of STRO-1, the essential mesenchymal stem cell marker, was found to decrease with advancing passages in few tissues. Because STRO-1 was identified and isolated from human gingiva, we were interested to know its status after a few passages. Human gingival mesenchymal stem cells (HGMSCs) were isolated fro...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27674
更新日期:2019-03-01 00:00:00
abstract::The signal transduction system that mediates bacterial chemotaxis allows cells to modulate their swimming behavior in response to fluctuations in chemical stimuli. Receptors at the cell surface receive information from the surroundings. Signals are then passed from the receptors to cytoplasmic chemotaxis components: C...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240510109
更新日期:1993-01-01 00:00:00
abstract::To ascertain whether p53 deficiency in vivo leads to the deregulation of DNA methylation machinery prior to tumor development, we investigated the expression profile of DNA methyltransferases in the thymus and the liver of p53(+/+), p53(+/-), and p53(-/-) mice at 7 weeks of age before tumor development. The expression...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20263
更新日期:2005-02-15 00:00:00
abstract::Craniosynostosis is a condition in which some or all of the sutures in the skull of an infant close prematurely. Fibroblast growth factor receptor 2 (FGFR2) mutations are a well-known cause of craniosynostosis. Many syndromes that comprise craniosynostosis, such as Apert syndrome, Crouzon syndrome, and Pfeiffer syndro...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.23368
更新日期:2012-02-01 00:00:00
abstract::During inflammation, overproduction of nitric oxide (NO) can damage chondrocytes. In this study, we separately evaluated the toxic effects of exogenous and endogenous NO on human chondrocytes and their possible mechanisms. Human chondrocytes were exposed to sodium nitroprusside (SNP), an NO donor, or a combination of ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.21268
更新日期:2007-08-15 00:00:00
abstract::Lack of guidelines for personalized chemotherapy treatment after surgery has caused gastric cancer (GC) patients' unnecessary exposure to toxicity and the financial burden of chemotherapy treatments. In our study, we aimed to identify potential biomarkers to predict GC patients' susceptibility to platinum-based on Gen...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28621
更新日期:2019-08-01 00:00:00
abstract::In the present study, we have investigated dexamethasone and insulin regulation of the expression of adipose-specific mRNA, namely, glycerophosphate dehydrogenase (G3PDH) and adipsin, at different stages of differentiation. During adipose conversion, insulin promotes an accumulation of G3PDH mRNA which is linked to ce...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240420407
更新日期:1990-04-01 00:00:00
abstract::An important aspect of the cellular differentiation in the intestine is the migration of epithelial cells from the crypt to the villus tip. As homeodomaine transcription factor CDX2 has been suggested to influence cell migration, we performed a genome-wide promoter analysis for CDX2 binding in the differentiated human...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22490
更新日期:2010-04-15 00:00:00
abstract::In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cells. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240450410
更新日期:1991-04-01 00:00:00
abstract::Vascular smooth muscle cells (SMCs), the major cellular constituent of an artery, synthesize the bulk of fibrillar collagens, including type V/XI, which regulates heterotypic collagen fibril assembly. Basic fibroblast growth factor (bFGF) is a heparin-binding polypeptide growth factor that has been implicated in impor...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/(sici)1097-4644(19980201)68:2<247::aid-jcb
更新日期:1998-02-01 00:00:00
abstract::Chondrocyte differentiation is a fundamental process during endochondral ossification. Several factors regulate maturation via the activity of downstream signaling pathways that target specific transcription factors and regulate chondrocyte-specific genes. In this study, we investigated the mechanisms involved in the ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.10677
更新日期:2003-12-15 00:00:00
abstract::Cervical cancer is one of the leading killers for female worldwide. Nevertheless, the less knowledge of molecular mechanism for cervical cancer limited the improvement of treatment effects. High-mobility group box 2 (HMGB2) belongs to the HMGB family, which could play diverse roles in cell proliferation. This work mai...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28998
更新日期:2019-10-01 00:00:00
abstract::Adenosine triphosphate (ATP), which plays a crucial role in both developing and mature cochleae, is released from greater epithelial ridge (GER) supporting cells of the rat cochlea, but the organelles in which ATP is stored have not yet been identified. Thus, we studied the organelles involved in ATP storage and sugge...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.29251
更新日期:2019-12-01 00:00:00
abstract::Extracts of Malus hupehensis (Pamp.) Rehder, containing flavonoids with good antioxidant and antiliver injury properties, possess various biological activities. The aim of this study was to explore the antioxidant activity of these extracts in vitro and in vivo. The antioxidant activity of the extracts was studied usi...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28469
更新日期:2019-02-19 00:00:00
abstract::Breast cancer resistance protein (BCRP) is an ATP-binding cassette multidrug transporter that confers resistance to various anticancer drugs like Mitoxantrone. Overexpression of BCRP confers multidrug resistance (MDR) in cancer cells and is a frequent impediment to successful chemotherapy. For stable reversal of BCRP-...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.21276
更新日期:2007-09-01 00:00:00
abstract::Intact human erythrocytes, initially depleted of Mg2+ by EDTA incubation in the presence of A23187, exhibit Mg(2+)-dependent phosphate production of around 1.5 mmol per liter cells.h, half-maximally activated at around 0.4 mM added free Mg2+. This appears to correspond to Mg(2+)-stimulated adenosine triphosphatase (Mg...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240460403
更新日期:1991-08-01 00:00:00
abstract::The cdk5/p35 complex has been implicated in a variety of functions related to brain development, including axonal outgrown and neuronal migration. In this study, by co-immunoprecipitation and pull-down experiments, we have shown that the cdk5/p35 complex associates with and phosphorylates the neuronal delta-catenin. I...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.21041
更新日期:2007-02-15 00:00:00
abstract::Butyrate inhibits growth of lung cancer. However, the molecular mechanism is still unclear. Here we profiled miRNAs that responded to sodium butyrate(NaB) stimulation in A549 cells, a non-small cell lung cancer cell line, using microarray. We found 33 up-regulated microRNAs and 22 down-regulated microRNAs (log2 ≥1.5 f...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.26547
更新日期:2018-04-01 00:00:00
abstract::This review point out several aspects regarding the mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase (Erk) network, which are still pending issues in the understanding how this pathway integrate information to drive cell fates. Focusing on the role of Erk during cell cycle, it has to be underline...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.22477
更新日期:2010-04-01 00:00:00
abstract::In this study, we purpose to investigate a novel five-gene signature for predicting the prognosis of patients with laryngeal cancer. The laryngeal cancer datasets were obtained from The Cancer Genome Atlas (TCGA). Both univariate and multivariate Cox regression analysis was applied to screening for prognostic differen...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.29535
更新日期:2019-10-31 00:00:00
abstract::microRNAs (miRNAs) are noncoding RNAs that regulates the expression of target messenger RNAs (mRNAs). c-FLIP is an inhibitor of cell apoptosis through inhibition of caspase 8. miR-150, miR-504, and miR-519d were related to cancer cell proliferation, invasion, and migration in colorectal cancer (CRC). However, the role...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28073
更新日期:2018-12-12 00:00:00
abstract::The role of TatD DNases as DNA repair enzymes or cell death (apoptotic) nucleases is well established in prokaryotes as well as eukaryotes. The current study aims to characterize the TatD nuclease from Bacillus anthracis (Ba TatD) and to explore its key histidine catalytic residues. Ba TatD was found to be a metal-dep...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28408
更新日期:2019-02-05 00:00:00
abstract::Endometrial carcinoma (EC) is one of the main gynecologic malignancies affecting women, but effective treatments are currently lacking. In the present study, we investigated the effect of sorafenib, a general kinase inhibitor, on several EC cell lines (HEC1A, HEC1B, and RL95-2). Sorafenib induced cell death in EC cell...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24530
更新日期:2013-08-01 00:00:00