Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells.

Abstract:

:In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cells. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to less than 10% HIV-1+ cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was less than 0.1%, indicating nonfaithful transmission of an unintegrated HIV-1 genome during clonal expansion. These results demonstrate that extrachromosomal HIV-1 DNA can contribute to the spread of HIV-1 infection and give rise to cells which have stably integrated HIV-1 provirus.

journal_name

J Cell Biochem

authors

Butera ST,Perez VL,Besansky NJ,Chan WC,Wu BY,Nabel GJ,Folks TM

doi

10.1002/jcb.240450410

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

366-73

issue

4

eissn

0730-2312

issn

1097-4644

journal_volume

45

pub_type

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