Abstract:
:The E6 protein of the high-risk human papillomavirus (HPV) types 16 and 18 is capable of complexing with the wild-type p53 tumor suppressor gene product, leading to loss of the normal p53 function as an anti-oncogene, whereas the low-risk HPV types 6 and 11 lack this binding property. The malignant potential of HPV 16 and 18 has been ascribed to this complexing of E6 with p53, which regularly leads to undetectable expression of the latter in HPV-positive lesions. To assess the role of p53 in HPV-associated genital carcinogenesis, the expression of p53 protein was studied immunohistochemically in 22 genital carcinomas and precancer lesions; 8 vulvar carcinomas, 1 VIN (vulvar intraepithelial neoplasia), 5 cervical carcinomas and 8 CIN (cervical intraepithelial neoplasia) using monoclonal antibody PAb 1801. Presence of HPV was demonstrated by PCR using HPV consensus primers, and amplified HPV-DNA was digested with the restriction enzymes giving distinct patterns for various HPV-types in gel electrophoresis. HPV-typing was confirmed by in situ hybridization with biotinylated DNA probes. Altogether, 17 of the 22 specimens (77%) showed p53 expression: 67% of the precancer lesions and 83% of carcinomas. Expression was more frequent (89%) in the vulvar than (70%) in cervical lesions. Using PCR,HPV DNA was detected in 19/22(86%) of the samples. The following HPV types were identified: HPV 6 (2 samples), HPV 11 (3 cases), HPV 16 (5 cases), HPV 33 (3 cases), and 6 contained unidentified HPV types. All HPV DNA-negative specimens showed p53 expression. Of the 19 HPV DNA-positive lesions, 5 were p53-negative, three of these being HPV 16 positive CIN lesions. The remaining two HPV 16 lesions were invasive carcinomas with a weak p53 expression. HPV 6 and 11-positive lesions showed a weak p53 expression more frequently than HPV-negative cases and HPV 33 lesions. The results indicate that p53 expression is detectable, but it is less frequent and less intense in HPV DNA-positive genital precancer lesions and carcinomas (particularly those with HPV 16 DNA) as compared with HPV DNA-negative lesions.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Tervahauta AI,Syrjänen SM,Väyrynen M,Saastamoinen J,Syrjänen KJsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
1107-11issue
4eissn
0250-7005issn
1791-7530journal_volume
13pub_type
杂志文章abstract:BACKGROUND:The aim of this study was to evaluate the historical cohort of 61 patients with carcinoma of the vulva, treated with radiation therapy from 1986 to 1997. PATIENTS AND METHODS:Twenty-seven patients were submitted to radiation therapy alone and 34 received radiotherapy post limited surgery in early stages and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND/AIM:The aim of the present study was to determine the efficacy of trabectedin combined with FOLFIRI (irinotecan, leucovorin and 5-fluorouracil) on a colorectal cancer (CRC) patient-derived orthotopic xenograft (iPDOX) mouse model. MATERIALS AND METHODS:A CRC tumor from a patient previously established in nu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13860
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:Green tea polyphenols (GTPs) have been proposed as promising candidates for chemoprevention. However, GTPs levels are maintained relatively low in the blood and are chemically-unstable. Lipid-soluble tea polyphenols (LTPs) are products of modified GTPs with ester linkage with fatty acids. LTPs are lipophilic...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:Canine lymphoma has lately drawn focus as a model of human non-Hodgkin's lymphoma due to its spontaneous occurrence and similar biological behavior. Cells with stem cell-like characteristics are believed to play a key role in therapeutic failure. Thus, an initial characterization and the possibility of speci...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-05-01 00:00:00
abstract::To evaluate the effects of dietary fats on breast cancer growth and metastasis, KPL-1 human breast carcinoma cells which have a propensity for axillary lymph node metastasis when inoculated into the thoracic mammary fat pad of female nude mice were examined. The mice were fed one of three semipurified diets containing...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::Total sialic acid (TSA) and "lipid-bound" sialic acid (LSA) were evaluated in comparison to carcinoembryonic antigen (CEA) and ferritin and neuron specific enolase (NSE) in 152 untreated patients with primary lung cancer, 107 benign pulmonary disease patients and 207 notmal controls. The mean concentrations of TSA, LS...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND:Concurrent chemoradiotherapy with gemcitabine improves median survival for patients with unresectable pancreatic cancer. Recently, hyperfractionated accelerated radiotherapy (HART) has been used to treat these patients; however, the safety and efficacy are not well defined. PATIENTS AND METHODS:The standard...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::In previous studies performed by our group, we observed that 2-deoxyglucose blocked the acidification of the medium used for culture of colon cancer cells caused by incubation with biguanides and it had an additive inhibitory effect on growth. In the present work, we found that 3-bromopyruvate can also prevent the low...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-02-01 00:00:00
abstract::Sprague-Dawley male rats, 24 days of age, were placed on diets based on a balanced ration as such and supplemented with Brazilian A rabica green coffee bean oil (0.10%), silymarin flavonolignans (0.10%), porcine trypsin (2429 mu/g ration) and ferrous sulfate (0.24% Fe) for a period of 32 weeks. A portion of the contro...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Thalidomide was recently suggested to be angiogenesis-inhibitor following the demonstration of its activity in a rabbit cornea micropocket model. The purpose of the present study was to test its efficacy in solid tumors in mice. B16-F10 melanoma and CT-26 colon carcinoma cells were injected subcutaneously, intravenous...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND/AIM:Several studies have revealed an association between single nucleotide polymorphisms (SNPs) in the VDR gene and prostate cancer (PCa) risk in European and Asian populations. To investigate whether VDR SNPs are associated with PCa risk in African-American (AA) men, nine VDR SNPs were analyzed in a case-co...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
abstract::The development of cisplatin drug resistance remains a chief concern in ovarian cancer chemotherapy. β-Elemene is a natural plant product with broad-spectrum antitumor activity towards many types of carcinomas. This study aimed to define the biological and therapeutic significance of β-elemene in chemoresistant ovaria...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract:UNLABELLED:The purpose of this study was to clarify the role and the predictive strength of the adhesion molecule CD44s (standard isoform) in colorectal carcinogenesis. MATERIALS AND METHODS:CD44s immunohistochemical expression was evaluated in 100 patients with colon adenoma and 100 patients with colon adenocarcinoma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::One hundred and twenty-one ovarian carcinomas were cultivated in soft agar according to the Courtenay & Mills (C-M) soft agar method. 71% of the tumours formed colonies, and 54% formed more than 30 colonies. Tumour cells from malignant fluids grew more frequently than did solid tumours, whereas the plating efficiencie...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND/AIM:In the present article we review on the use of Volumetric Modulated Arc Therapy (VMAT) for a small lung nodule that was centrally located in close proximity to the mediastinal structures. CASE REPORT:An inoperable patient with central, clinical stage IA adenocarcinoma of the right lung was treated with ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2013-10-01 00:00:00
abstract::The interaction between sodium 5,6-benzylidene-L-ascorbate (SBA) and dopamine was investigated by three different parameters: radical intensity, prooxidant action and cytotoxic activity. Under alkaline conditions, SBA and dopamine produced doublet and quartet ESR signals, respectively. The addition of increasing conce...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Flow cytometric DNA analysis was performed in 67 tubular, tubulovilous and villous rectal adenomas with different degree of dysplasia. One of the adenomas contained a focus of early invasive carcinoma. All but one of the 67 adenomas had near diploid DNA histograms; the exception was a tubular adenoma with severe dyspl...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Metals and metal compounds have been used in medicine for several thousands of years. In this review we summarized the anti-cancer activities of the ten most active metals: arsenic, antimony, bismuth, gold, vanadium, iron, rhodium, titanium, gallium and platinum. The first reviewed metal, arsenic, presents the anomaly...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Ano-genital carcinoma is a polyfactorial and polygenic disease. Certain strains of human papillomavirus (HPV) have been detected in a high percentage of patients. It has been suggested that p53 polymorphisms may be relevant for the interaction with viral proteins that inactivates p53. MATERIALS AND METHODS:...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Angiosarcoma of the breast is a malignant tumour of vascular endothelial cells. It is a rare and difficult tumour to treat. The authors report a case of cutaneous radiation-associated angiosarcoma treated with paclitaxel chemotherapy. A few days after drug administration, bleeding of skin lesions was observed and the ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::Around 1970 it was assumed that tumour cells are due to a single specific oncogenic mutation. This implied that daily many thousands of tumour cells would arise de novo and that most of these tumour cells were killed by immune surveillance or natural resistance mechanisms. Recent findings on immune surveillance and na...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Although some types of intestinal metaplasia are considered to be potentially pre-malignant lesions leading to gastric carcinoma, their high frequency and variability limit their usefulness as early markers of malignant progression. The present study was carried out to search for parameters which could be used in conj...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND/AIM:As a result of activation of transcription factors engaged in epithelial-mesenchymal transition (EMT), such as Twist, inhibition of epithelial markers and an increased expression of mesenchymal markers are observed. One of the specific markers of cancer-associated fibroblasts is podoplanin (PDPN) - a muc...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11978
更新日期:2017-10-01 00:00:00
abstract::Proteolytic enzymes are believed to be necessary for tumor cell invasion. We have studied the effects of the cysteine proteinase inhibitor E-64 and the serine and cysteine proteinase inhibitor leupeptin, on the ability of human malignant melanoma cells (LOX) to pass through an artificial basement membrane. Transwell c...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract:AIM:Metabolic syndrome has been identified as a risk factor for colorectal cancer and adenoma. The aim of our study was to assess the risk of colorectal cancer and adenoma in an adult Italian population with metabolic syndrome. PATIENTS AND METHODS:Ninety patients with metabolic syndrome were prospectively compared ag...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract::An in vitro cell line (HT 58) has been established from a human (B) NHL xenograft. The lymphoma cells in culture retained their lymphoblastic appearance, DNA-content, IgM/lambda monoclonality and many immunophenotypic markers. The clonal chromosomal abnormalities involved the chromosomes 1, 2, 3 and 14. The cells expr...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::In the present work, we studied the expression of the c-myc oncoprotein p-62 and the ras oncoprotein p-21 in the dermal cellular infiltrate of paraffin embedded skin specimens, obtained from patients suffering from Mycosis Fungoides and Sezary syndrome. Nineteen specimens from early stage Mycosis Fungoides, nineteen f...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The M14 human melanoma cell line was characterized either for some biological features in vitro and in vivo or for responsiveness to a panel of anti-tumor agents of clinical interest. The results of our study show that the M14 cells in culture resemble the in vivo melanomas both biologically and functionally, thus sug...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::We have previously shown biopsies from human malignant melanoma, as well as a cell line (MELUR) derived from human malignant melanoma cells, to contain dexamethasone (DEX) - and medroxyprogesterone acetate (MPA) - binding macromolecules exhibiting properties of glucocorticoid receptors from well-known target organs. I...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Intraosseous lipomas are rare benign primary bone tumors, with an incidence of one per thousand bone tumors. We studied eleven cases of intraosseous lipoma of the calcaneus. All the patients received radiographic examinations and MRI with T1-and T2-weighted images with Gd-DTPA enhancement. Seven patients received tumo...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00