Clinical value of the flow cytometric method for measuring lymphocyte subset activation: spontaneous activation of T-cell subpopulations is associated with acute GvHD.


:Thymidine ((3)H-TdR) incorporation remains the most commonly used method to quantifying T-cell proliferation. This method, however, does not provide information about specific lymphocyte subpopulations responding to different stimuli. In our study, we modified previously described nonradioactive flow-cytometric T-cell activation assay measuring the expression of a CD69+ antigen on T-cell subsets and applied it to analysis of lymphocyte subsets activation/proliferation in children after allogeneic hematopoietic cell transplantation (HCT). We compared the percentage of spontaneously activated lymphocyte subpopulations (background) and the percentage of PHA-P, PWM, and SEB-stimulated cell subsets from two groups of patients: group 1, children with Graft versus Host Disease (GvHD) and group 2, children without any signs of GvHD at the time of analysis. High rate of spontaneous T-cell subset activation was found in group 1 with CD3+CD8+Ts cells being the most affected cell population. High background activation of Th and B cells correlated with the occurrence of autoimmune phenomena posttransplant. Rapid quantification of CD69+ expression on unstimulated and stimulated T-cell subsets proved to be a valuable method for monitoring children after allogeneic HCT. High proportion of activated, unstimulated Ts cells observed in the GvHD group may underline the critical role of CD3+CD8+ cells in the pathogenesis of GvHD. Thus in future immunosuppressive therapy may be adjusted according to the proportion of activated Ts cells.


Transplant Proc


Kalwak K,Turkiewicz D,Ussowicz M,Gorczyńska E,Toporski J,Ryczan R,Rybka B,Noworolska-Sauren D,Chybicka A




Has Abstract


2003-06-01 00:00:00














  • Graft survival with high levels of cytotoxic antibodies.

    abstract::In this study, pretransplant transfusions significantly improved the survival of first cadaver kidney transplants. Large numbers of transfusions had the same influence on graft survival as small number of transfusions. Graft survival was not influenced by pretransplant antibody levels, and the beneficial effect of tra...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Feduska NJ,Amend WJ,Vincenti F,Perkins HA,Opelz G,Terasaki PI,Iwaki Y,Duca R,Dodge D,Salvatierra O

    更新日期:1981-03-01 00:00:00

  • Switching from mycophenolate mofetil to enteric-coated mycophenolate sodium in liver transplant patients with gastrointestinal complications.

    abstract:OBJECTIVE:Our aim was to safely and effectively reduce adverse gastrointestinal (GI) events resulting from the use of mycophenolate mofetil (MMF) in liver transplant patients by switching to enteric-coated mycophenolate sodium (EC-MPS). PATIENTS AND METHODS:We studied 19 patients on maintenance therapy presenting with...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Barrera-Pulido L,Alamo-Martínez JM,Marín-Gómez LM,Suárez-Artacho G,Bernal-Bellido C,Domínguez-Usero D,Tallón-Aguilar L,Pareja-Ciuró F,Sousa-Martín JM,García-González I,Gómez-Bravo MA

    更新日期:2009-07-01 00:00:00

  • Pretreatment with the tumor nerosis factor-alpha blocker etanercept attenuated ischemia-reperfusion renal injury.

    abstract:INTRODUCTION:Tumor necrosis factor (TNF)-alpha mediates inflammation and apoptosis in ischemia-reperfusion (IR) injury of the kidneys. Etanercept, a soluble TNF-alpha receptor, has shown anti-inflammatory and anti-apoptotic effects in several animal models of renal injury, including chronic insufficiency and unilateral...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Choi DE,Jeong JY,Lim BJ,Na KR,Shin YT,Lee KW

    更新日期:2009-11-01 00:00:00

  • Posttransplant complications encountered in renal transplantation in the Middle East.

    abstract::This paper discusses the commonly seen complications encountered in Middle Eastern transplant recipients, including posttransplant Kaposi's sarcoma, tuberculosis, infective diarrhea, and pregnancy. ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Al-Khader AA,Shaheen FA

    更新日期:2004-01-01 00:00:00

  • Impact of immunosuppression on the development of Epstein-Barr virus (EBV) viremia after pediatric liver transplantation.

    abstract:OBJECTIVES:Pediatric liver transplant (OLT) patients are at risk of posttransplant lymphoproliferative disease (PTLD) from Epstein-Barr virus (EBV). This study examined the impact of induction and immunosuppression on EBV viremia. METHODS:A retrospective chart review was performed on 197 pediatric patients and inducti...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lu BR,Park KT,Hurwitz M,Cox KL,Berquist WE

    更新日期:2013-01-01 00:00:00

  • Clinical significance of prophylactic antibiotics in renal transplantation.

    abstract:INTRODUCTION:The use of new selective immunosuppressants as well as the emergence of new antimicrobial resistances raise the use of prophylactic antibiotics as a matter of controversy. The purpose of this study was to retrospectively analyze the clinical significance of prophylactic antibiotics in kidney transplantatio...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Choi SU,Lee JH,Oh CK,Shin GT,Kim H,Kim SJ,Kim SI

    更新日期:2013-05-01 00:00:00

  • Outcome of Living Donor Transplant Kidneys With Multiple Arteries.

    abstract:BACKGROUND:Living donor transplantation (LDT) using kidneys with multiple arteries (MA) has previously been reported to be associated with increased complications and poorer outcomes in recipients. The objective of this study was to investigate outcomes of LDT with MA at the National University Hospital of Singapore, a...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lim YM,Han X,Raman L,Ng TK,Goh TH,Vathsala A,Tiong HY

    更新日期:2016-04-01 00:00:00

  • Nonmarginal-donor duodenal ulcers caused by rejection after simultaneous pancreas and kidney transplantation: a case report.

    abstract:OBJECTIVE:Pancreas transplantation has been associated with the highest surgical complication rate among routinely performed organ transplant procedures. Complications can be caused not only from the pancreas itself but also from the simultaneously transplanted duodenum: gastrointestinal bleeding, duodenal ulcer, pseud...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Miyagi S,Sekiguchi S,Kawagishi N,Akamatsu Y,Satoh K,Takeda I,Fujimori K,Satomi S

    更新日期:2011-11-01 00:00:00

  • Conversion to proliferation signal inhibitors-based immunosuppressive regimen in kidney transplantation: to whom and when?

    abstract:BACKGROUND:Despite significant advances in kidney transplantation, long-term graft survival has not dramatically improved leading to strategies to change immunosuppression during the posttransplantation period. Proliferation signal inhibitors (PSI) sirolimus or everolimus possess immunosuppressive and antiproliferative...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sahin S,Gürkan A,Uyar M,Dheir H,Turunç V,Varilsuha C,Kaçar S

    更新日期:2011-04-01 00:00:00

  • An audit of a steriod withdrawal regimen in cardiac transplantation patients.

    abstract:BACKGROUND:Heart transplantation is optimal treatment for many patients with end-stage heart failure. Current data report 1-year graft survival rates of 85% after transplantation. The success of transplantation in large part is attributable to immunosuppression, including steroids, one of the mainstay agents. Despite i...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Nawaz MA,Curry P,Patni R,Punjabi P,Murday A

    更新日期:2011-03-01 00:00:00

  • Basiliximab (Simulect) reduces acute rejection among sensitized kidney allograft recipients.

    abstract:AIMS:Our goal was to evaluate the efficacy and safety of basiliximab (Simulect) as immunosuppressive induction therapy for the prevention of acute allograft rejection among sensitized kidney recipients. METHODS:Fifty-six adult recipients receiving cadaveric kidney transplant with panel reactive antibody class I rangin...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Tan J,Yang S,Wu W

    更新日期:2005-03-01 00:00:00

  • New strategies of cyclosporine monitoring in heart transplantation: initial results.

    abstract::The aim of this study was to evaluate cyclosporine (CyA) absorption profiles in heart transplantation to establish the most adequate monitoring strategy and determine the optimal therapeutic range for AUC(0-4) or C2 levels. A total of 22 full pharmacokinetic studies were performed at steady-state in 22 adult heart tra...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Arizón del Prado JM,Aumente Rubio MD,Cárdenas Aranzana M,López Malo de Molina MD,Segura Saint-Gerons J,López Granados A,Rodríguez Esteban E,Ruiz Ortiz M,Romo Peñas E,Muñoz Carvajal I,González Rodríguez JR,Segura Saint-Gerons C,Va

    更新日期:2003-08-01 00:00:00

  • Gene transfer using recombinant simian virus 40 viral vectors into mice bone marrow progenitor cells depressed the immunogenicity of keratinocyte stem cells.

    abstract::Hematopoietic stem cell (HSC) gene transfer has been attempted almost entirely ex vivo and has been limited by loss of self-renewal capacity and transplantation-related defects in homing and engraftment. Herein we have attempted to overcome these limitations by injecting vectors directly into the bone marrow (BM) to t...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Li W,Xu T,Wang JF,Wu XF,Li M,Lu PY

    更新日期:2008-12-01 00:00:00

  • Pretreatment Combination Reduces Remote Organ Damage Secondary to Intestinal Reperfusion Injury in Mice: Follow-up Study.

    abstract:BACKGROUND:Intestinal ischemia-reperfusion injury occurs after different surgical treatments, including intestinal transplantation. This harmful process may have an effect in remote organs, leading to multiple organ dysfunction syndrome and death. Therefore, to establish strategies to attenuate local and remote damage ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Stringa P,Lausada N,Romanin D,Portiansky E,Zanuzzi C,Machuca M,Gondolesi G,Rumbo M

    更新日期:2016-01-01 00:00:00

  • Decompressive craniectomy and brain death prevalence and mortality: 8-year retrospective review.

    abstract::Decompressive craniectomy (DC) is a surgical practice that has been used since the late 19th century. The cerebral blood flow increase after the performance of a DC can delay and even prevent the development of cerebral circulatory arrest and brain death (BD). We aimed to determine the prevalence of BD, the use of DC,...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Pereyra C,Benito Mori L,Schoon P,Violi D,Jacintho P,Segui G,Losio D,Lugaro M,Benavent G,Prieto M,Strati J,Diaz G

    更新日期:2012-09-01 00:00:00

  • Kidney Donor Risk Index Score Is More Reliable Than Kidney Donor Profile Index in Kidney Transplantation From Elderly Deceased Donors.

    abstract:BACKGROUND:It is unclear whether both Kidney Donor Profile Index (KDPI) and Kidney Donor Risk Index (KDRI) scores can be applied to elderly deceased donors (DDs). This study aimed to compare the predictive values of KDRI and KDPI for the occurrence of delayed graft function (DGF) in kidney transplantation (KT) from eld...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Jun H,Yoon HE,Lee KW,Lee DR,Yang J,Ahn C,Han SY,Korean Organ Transplantation Registry Study Group.

    更新日期:2020-01-01 00:00:00

  • Improvement in the care of patients with kidney transplant failure: recommendations of the Spanish Society of Nephrology.

    abstract::Although long-term outcomes have improved, graft loss caused by chronic allograft nephropathy remains an important obstacle. This situation, together with the progressive increase in the number of renal transplant patients, means that the population of transplant patients readmitted to a dialysis program will be progr...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Fernandez Fresnedo G

    更新日期:2009-07-01 00:00:00

  • Sirolimus in renal transplantation: analysis of safety and efficacy in a nonprotocol conversion group.

    abstract:BACKGROUND:Conversion to sirolimus (SRL)-based immunosuppression in renal transplant recipients is an alternative for chronic allograft dysfunction (CAD), cancer and viral infections. We sought to analyze the indications for and safety and efficacy of conversion to SRL among renal transplant patients. METHODS/MATERIAL...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Gois PH,Rivelli GG,Pereira LM,Mazzali M

    更新日期:2012-10-01 00:00:00

  • Does etiology of end-stage renal disease affect sleep quality in kidney transplant recipients?

    abstract:BACKGROUND:That hypertension (HTN) as a leading cause of end-stage renal disease (ESRD) is linked to sleep disorders in the general population can be the basis of a hypothesis that HTN may be a contributing factor to the poor quality of sleep in some kidney transplant recipients. This study was designed to investigate ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Ameli J,Kachuee H,Assari S,Rasta VR,Khoddami-Vishte HR,Einollahi B,Lessan-Pezeshki M,Khedmat H

    更新日期:2007-05-01 00:00:00

  • Is it safe to use a kidney from an expanded criteria donor?

    abstract:BACKGROUND:The use of expanded criteria donor (ECD) kidneys has been encouraged to enlarge the donor pools due to the shortage of donors. However, a major concern with ECD kidneys is poor long-term graft survival. The objective of this study was to determine whether ECD kidneys had a negative impact on graft survival. ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Kim JM,Kim SJ,Joh JW,Kwon CH,Song S,Shin M,Kim BN,Lee SK

    更新日期:2011-07-01 00:00:00

  • Diagnosis of renal allograft rejection and acute tubular necrosis by 99mTc-mononuclear leukocyte imaging.

    abstract::One hundred kidney transplant recipients were evaluated on the first and fifth days after transplantation by Tc-99m mononuclear cell scintigraphy. We have developed a quantitative method to diagnose rejection and acute tubular necrosis (ATN) by comparing regions of interest drawn on allograft scintigraphs at different...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lopes de Souza SA,Barbosa da Fonseca LM,Torres Gonçalves R,Salomão Pontes D,Holzer TJ,Proença Martins FP,Gutfilen B

    更新日期:2004-12-01 00:00:00

  • Donor-recipient age difference and graft survival in living donor kidney transplantation.

    abstract:BACKGROUND:In paired living kidney exchange donation from an old donor to a young recipient, it may be argued that elderly donors provide an inferior quality kidney. However, the impact of donors older than recipients on transplant outcomes remains unclear. METHODS:We retrospectively reviewed the charts of primary liv...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lee YJ,Chang JH,Choi HN,Jung JY,Kim YS,Chung WK,Park YH,Lee HH

    更新日期:2012-01-01 00:00:00

  • Severely elevated intrarenal arterial impedance and abnormal venous flow pattern in a normal functioning kidney graft.

    abstract::Serial Doppler sonographic impedance measurements represent the most common diagnostic method for noninvasive monitoring of kidney grafts. Severely elevated arterial impedance is almost always associated with graft dysfunction. However, we describe in the present work a renal transplant recipient with optimal graft fu...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,评审


    authors: Salgado O,García R,Henríquez C,Rosales B,Sulbarán P

    更新日期:2003-08-01 00:00:00

  • Anatomical classification of the peripheral right hepatic duct: early identification of a preventable source of morbidity and mortality in adult live donor liver transplantation.

    abstract:INTRODUCTION:The purpose of this study was to determine the impact of our classification on right graft adult live donor liver transplantation (ALDLT) outcomes. METHODS:Three-dimensional computed tomography (CT) reconstructions were used to classify the hilar and sectorial biliary anatomy of 71 consecutive live liver ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Radtke A,Sgourakis G,Sotiropoulos GC,Molmenti EP,Fouzas I,Schroeder T,Saner FH,Schenk A,Beckebaum S,Malagó M,Lang H

    更新日期:2008-11-01 00:00:00

  • Transient ischemic attack after rizatriptan administration in a liver transplant recipient: a case report.

    abstract::We report the case of a male liver transplant recipient who developed de novo migraine while on tacrolimus therapy. Considering the inadequate control of pain using nonsteroidal antiinflammatory drugs, rizatriptan benzoate (10 mg orally) was administered (double administration). After both administrations a clinically...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Curro G,Baccarani U,Adani GL,Lorenzin D,Bresadola F

    更新日期:2006-11-01 00:00:00

  • Comparison of endothelial nitric oxide synthase and endothelin-1 levels in kidneys removed from living pigs after cardiac arrest and brain death.

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Oczkowicz G,Caban A,Budziński G,Suszka-Świtek A,Dolińska B,Czech E,Wiaderkiewicz R,Ryszka F,Cierpka L

    更新日期:2014-10-01 00:00:00

  • Transplant Friends: an interactive education program for patients awaiting kidney transplantation.

    abstract:BACKGROUND:Organ transplantation is the preferred treatment for end-stage renal disease. Renal transplant recipients are surviving longer with a better quality of life. Although many hospitals have transplant education programs in place, transplant patients indicate that there is a need for additional information. Tran...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sharkey C,Gourishankar S

    更新日期:2003-11-01 00:00:00

  • The colon displays an absorptive capacity of tacrolimus.

    abstract:PURPOSE:In our previous study, blood levels of orally administered tacrolimus were significantly higher in short bowel piglets than in control animals. It has been reported that the blood levels of tacrolimus are influenced by the metabolic activity of intestinal CYP3A4. If tacrolimus may be absorbed in the colon, dire...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Nishi K,Ishii T,Wada M,Amae S,Sano N,Nio M,Hayashi Y

    更新日期:2004-03-01 00:00:00

  • Guidelines for prevention and management of complications following kidney transplantation in rats.

    abstract::Kidney transplantation in rats is a useful model for microsurgery, transplantation, and immunology studies. Our aim was to analyze various techniques of kidney transplantation in rats with emphasis on guidelines for the prevention and management of complications. Complications were categorized into general, vascular, ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Pahlavan PS,Mehrabi A,Kashfi A,Soleimani M,Fani-Yazdi SH,Schemmer P,Gutt CN,Friess H,Weitz J,Kraus TW,Büchler MW,Schmidt J

    更新日期:2005-06-01 00:00:00

  • Initial clinical effect of intraportal insulin administration on liver graft regeneration in adult patients underwent living donor right lobe liver transplantation.

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    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Xu MQ,Yan LN,Li B,Wen TF,Zeng Y,Zhao JC,Wang WT,Yang JY,Ma YK,Chen ZY,Zhang ZW

    更新日期:2009-06-01 00:00:00