当前位置： SCI文献检索 > TRANSPLANTATION PROCEEDINGS期刊下所有文献 > Cryopreservation of freshly isolated porcine islet cells.

Cryopreservation of freshly isolated porcine islet cells.

Abstract：

INTRODUCTION:The use of xenogenic islet cells may be a possibility to overcome the shortage of human donor organs to treat diabetes. Microencapsulation seems to be a promising method for immunoprotection. Since isolation, purification, encapsulation, and transplantation of islet cells are labor intensive, cryopreservation has emerged as an attractive system of islet banking. The aim of this study was to determine the influence of three different freezing media (FM) on viability of freshly isolated porcine islet cells (FIPIC). METHODS:FIPIC were isolated using a modified Ricordi method and purification performed using a Lymphoprep density gradient. Viability of FIPIC prior to freezing and after thawing was determined using the MTT-based Cell Growth Determination Kit. Insulin production was detected using enzyme-linked immunosorbent assay. Three different FM containing dimethylsulfoxide (DMSO) or glycerol and sucrose were used for cryoprotection of FIPIC. RESULTS:Isolation and purification of FIPIC resulted in 95% +/- 1.3% viability and 97% +/- 1.4% purity. Cryopreservation with FM I (containing DMEM, FCS, DMSO) yielded 98.4% and FM III (containing DMEM, FCS, glycerol) 93.1% viability, whereas only 85.6% were alive when cryoprotection is performed with FM II (containing DMSO, BM). Glucose stimulation revealed a loss of 2.8% and 1.9% of insulin secretion per microgram DNA when working with FM I and FM III, but a decrease in glucose-dependent insulin secretion of 7.8% (P < .05) when FIPIC were stored in FM II. DISCUSSION:Low concentrations of DMSO or the use of glycerol and sucrose seem to be equivalent to cryopreserve FIPIC.

journal_name

Transplant Proc

journal_title

Transplantation proceedings

authors

Stiegler P,Stadlbauer V,Schaffellner S,Florian H,Lackner C,Iberer F,Tscheliessnigg K

doi

10.1016/j.transproceed.2007.02.076

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

1609-11

issue

5

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(07)00333-8

journal_volume

39

pub_type

杂志文章

相关文献

TRANSPLANTATION PROCEEDINGS文献大全
  • Immunoprophylaxis with Simulect (basiliximab) in kidney transplantation: results from routine clinical practice at 18 kidney transplant units.

    abstract：OBJECTIVE:The objective of this study is to assess a Simulect (basiliximab) regimen in routine clinical practice in the Spanish kidney transplantation units to evaluate efficacy and safety. METHODS:In this prospective, observational study, data on demographics, parameters of efficacy, and safety in patients who under ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,多中心研究

    doi：10.1016/s0041-1345(03)00575-x

    authors： Rengel M,Fernández Rodriguez A,Gómez Huertas E,Plaza JJ,Ruiz San Millán JC,Oppenheimer F,Bustamante J,Checa D,Sanchez Plumed JA,García Pérez J,Sanz Guajardo A,Llorente S,Martin Govantes JA,Gentil MA,Gomez Ullate P,Sanchez G

    更新日期：2003-08-01 00:00:00

  • Successful Ultrasound-Guided Percutaneous Drainage of Multiple Splenic Abscesses in a Kidney Transplant Patient With Encapsulated Sclerosing Peritonitis: A Case Report.

    abstract：:Differential diagnosis of post-transplant infections should include rare/uncommon foci and pathogens. We present a rare case of life-threatening infection, a splenic abscess in a 53-year-old woman who was transplanted with a cadaveric kidney 5 months previously. The patient was admitted to our clinic with chills, shiv...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2015.04.063

    authors： Tuğcu M,Ruhi Ç,Boynueğri B,Kasapoğlu U,Can Ö,Kiliçoğlu G,Şahin G,Titiz M

    更新日期：2015-06-01 00:00:00

  • A Case Report of the Resolution of Multiple Recalcitrant Verrucae in a Renal Transplant Recipient After a Mycophenolate Mofetil Dose Reduction.

    abstract：:Renal transplant recipients are at an increased risk of developing verrucae due to chronic immunosuppression, and certain therapies may confer a greater risk. Herein, we describe a 51-year-old woman with a 10-year-old unrelated kidney transplant who developed numerous therapy-resistant verrucae while on mycophenolate ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2016.11.030

    authors： Ash MM,Jolly PS

    更新日期：2017-01-01 00:00:00

  • Renal amyloidosis followed more than 5 years: report of 12 cases.

    abstract：:Renal involvement with amyloidosis is common but causes patient survival to be poor, rarely reaching 5 years. In this study, we retrospectively reviewed clinical and biological characteristics as well as treatments and outcomes of patients with renal amyloidosis followed for more than 5 years. Between 1975 and 2003, 4...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2004.07.022

    authors： Kaaroud H,Boubaker K,Béji S,Abderrahim E,Moussa FB,Turki S,Goucha R,Hedri H,El Younsi F,Kheder A,Maiz HB

    更新日期：2004-07-01 00:00:00

  • Can liver transplantation achieve similar effects at high altitudes compared with plains: case report.

    abstract：:In orthotopic liver transplantation, particular emphasis must be placed on the unique physiologic, pathologic, and clinical features in residents living in areas at high vs low altitude. Hypobaric hypoxia, hypothermia, heavy radiation, high wind speed, and superevaporation at high altitudes may lead to various disease...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2009.01.115

    authors： Jin S,Chen Z,Lunan Y,Zeng Y,Wen T,Li B,Zhao J,Wang W,Xu M,Yang J

    更新日期：2009-06-01 00:00:00

  • Effects of polyethylene glycol and hydroxyethyl starch in University of Wisconsin preservation solution on human red blood cell aggregation and viscosity.

    abstract：:University of Wisconsin (UW) preservation solution is considered an effective flush and cold storage liquid. However, recent studies have provided evidence of the hyperaggregating effect on human red blood cells (RBC) of hydroxyethyl starch (HES), one of the components of the UW solution. In contrast, preservation sol...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2006.02.068

    authors： Mosbah IB,Franco-Gou R,Abdennebi HB,Hernandez R,Escolar G,Saidane D,Rosello-Catafau J,Peralta C

    更新日期：2006-06-01 00:00:00

  • Very low-grade proteinuria at 3 months posttransplantation is an earlier marker of graft survival.

    abstract：:Proteinuria is an early finding that appears in the first 3 months after transplantation in half of patients. Frequently, it is very low grade (VLP; <0.5 g/24 h). The aim of this study was to analyze the risk factors and prognostic significance of VLP at 3 months posttransplantation, which was maintained for the first...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2009.06.137

    authors： Sancho Calabuig A,Pallardó Mateu LM,Avila Bernabeu AI,Gavela Martínez E,Beltrán Catalán S,Crespo Albiach JF

    更新日期：2009-07-01 00:00:00

  • Analysis of vascular complications after renal transplantation.

    abstract：PURPOSE:Despite medical and surgical advances, vascular complications remain common after renal transplant, occurring among 3%-15% of patients. These complications may compromise graft function. This study sought to evaluate the frequency and management of vascular complications after renal transplant. MATERIALS AND M...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2011.01.007

    authors： Aktas S,Boyvat F,Sevmis S,Moray G,Karakayali H,Haberal M

    更新日期：2011-03-01 00:00:00

  • Neuropsychological assessment in kidney and liver transplantation candidates.

    abstract：INTRODUCTION:Chronic renal and liver diseases are associated with cognitive and intellectual impairment, which can be irreversible even after kidney or liver transplantation. OBJECTIVE:We sought to investigate the presence of cognitive deficits in organ transplantation candidates. METHODS:From May 2005 to March 2006,...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.02.042

    authors： Lacerda SS,Guimaro MS,Prade CV,Ferraz-Neto BH,Karam CH,Andreoli PB

    更新日期：2008-04-01 00:00:00

  • The significance of antiphospholipid antibodies in liver recipients.

    abstract：BACKGROUND:The presence of antiphospholipid antibodies (APLAs) may be associated with increased thrombotic risk. Liver graft thrombosis may necessitate retransplantation. AIM:To determine the prevalence of APLAs among liver recipients and to investigate the relationship between APLAs and liver graft thrombosis. MATER...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2013.01.024

    authors： Furmańczyk-Zawiska A,Tronina O,Baczkowska T,Chmura A,Durlik M

    更新日期：2013-06-01 00:00:00

  • Posttransplant diabetes mellitus: incidence and risk factors.

    abstract：UNLABELLED:Posttransplant diabetes mellitus (PTDM) is common post transplantation and is associated with tacrolimus (TAC) and steroid therapy. The aim of the present study was to analyze the incidences of PTDM and associated risk factors. METHODS:We selected renal transplant recipients treated with TAC, mycophenolate ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.03.018

    authors： Mazali FC,Lalli CA,Alves-Filho G,Mazzali M

    更新日期：2008-04-01 00:00:00

  • Beneficial effect of tetrahydrobiopterin on the survival of rats exposed to hepatic ischemia-reperfusion injury.

    abstract：INTRODUCTION:The protective role of nitric oxide (NO) against hepatic ischemia-reperfusion (I/R) injury remains controversial. In this study we investigated the effect of tetrahydrobiopterin (BH4) on the survival of rats exposed to an hepatic I/R injury. METHODS:The rats were subjected to 100 minutes of 70% hepatic is...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2004.12.051

    authors： Hara Y,Teramoto K,Kumashiro Y,Sato E,Nakamura N,Takatsu S,Kawamura T,Arii S

    更新日期：2005-01-01 00:00:00

  • Assessing the risk of infection and rejection in Hispanic renal transplant recipients by means of an adenosine triphosphate release assay.

    abstract：INTRODUCTION:Because of the necessary immunosuppression, transplant recipients have a high risk of infection. Conversely, underimmunosuppression carries with it the risk of rejection. It would be quite useful to have a test that could differentiate between infection and rejection in renal transplant patients and better...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2006.02.051

    authors： Cadillo-Chávez R,de Echegaray S,Santiago-Delpín EA,Rodríguez-Trinidad AT,Camacho-Carrazo B,Alfaro T,Saavedra-Pozo M,Carrasquillo L,González-Caraballo ZA,Morales-Otero LA

    更新日期：2006-04-01 00:00:00

  • Utilization of HbA1c in Screening Living Kidney Donors With Prediabetes.

    abstract：OBJECTIVES:To study the outcome of living kidney donors with prediabetes and to evaluate the utilization of baseline HbA1c to identify donors at high risk for developing diabetes during the postdonation follow-up period. PATIENTS AND METHODS:Living kidney donors with prospectively collected preoperative fasting glucos...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2019.03.082

    authors： Na JC,Yoon MG,Lee HH,Yoon YE,Kim DG,Huh KH,Kim YS,Han WK

    更新日期：2019-10-01 00:00:00

  • Cellular immunoisolation for islet transplantation by a novel dual porosity electrospun membrane.

    abstract：:Immunoisolation strategies have the potential to impact the treatment of several diseases, such as hemophilia, Parkinson's and endocrine disorders, such as parathryroid disorders and diabetes. The hallmark of these disease states is the amelioration of the disease process by replacement of the deficient protein. Natur...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2011.10.031

    authors： Krishnan L,Clayton LR,Boland ED,Reed RM,Hoying JB,Williams SK

    更新日期：2011-11-01 00:00:00

  • Living related liver transplantation for hepatocellular carcinoma in Egypt.

    abstract：BACKGROUND:Living related liver transplantation (LRLT) for hepatocellular carcinoma (HCC) in cirrhotic patients has emerged as a rewarding therapy for a cure. Extensions of the Milan criteria have been proposed with encouraging results. PATIENTS AND METHODS:From October 2001 to June 2004, 47 adult patients with end-st...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2005.08.037

    authors： El-Meteini M,Fayez A,Fathy M,Abdalaal A,Safaan H,Mostafa I,Abdalaal M,Mokhtar A,Salah M,El-Dorry A,Abdalwahab S,El-Monayeri M,Boillot O

    更新日期：2005-09-01 00:00:00

  • Analysis of liver transplantation outcome in patients with MELD Score > or = 30.

    abstract：:Since July 2006, the liver graft allocation has been changed from the waiting time to the Model for End-stage Liver Disease (MELD), prioritizing the sickest patients, who have a higher risk of dying on the waiting list, and sometimes in such poor clinical condition that it compromises transplantation outcomes. The aim...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.03.016

    authors： Ferraz-Neto BH,Zurstrassen MP,Hidalgo R,Meira-Filho SP,Rezende MB,Paes AT,Afonso RC

    更新日期：2008-04-01 00:00:00

  • Cholangiocarcinoma, 10-Year Survival After Liver Transplantation: A Case Report.

    abstract：:Cholangiocarcinoma is the second most common neoplasm in the liver, with a very poor, short-term prognosis. Today, surgery associated with or without an adjuvant is the only curative treatment. Liver transplantation (LT) is the best treatment for hepatocellular carcinoma tumor. In recent years, treatment of hilar chol...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2019.12.030

    authors： Herrero Torres MA,Dominguez Bastante M,Molina Raya A,Palomo Lopez I,Santoyo Villalba J,Villegas Herrera MT,Becerra Massare A,Brea Gomez E,Zambudio Carroll N,Villar Del Moral JM

    更新日期：2020-03-01 00:00:00

  • Risk factors and prognosis of patients with primary graft failure after heart transplantation: an Asian center experience.

    abstract：BACKGROUND:Although primary graft failure (PGF) after heart transplantation is a feared complication, most reports have come from Western countries. We analyzed the incidence in our hospital and tried to determine the predictive risk factors for PGF that require mechanical circulatory support or high dosage of inotropi...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2013.11.107

    authors： Chen JW,Chen YS,Chi NH,Huang SC,Yu HY,Chou NK,Wang CH,Wang SS

    更新日期：2014-04-01 00:00:00

  • Comparison of Sirolimus Combined With Tacrolimus and Mycophenolate Mofetil Combined With Tacrolimus in Kidney Transplantation Recipients: A Meta-Analysis.

    abstract：PURPOSE:The study was designed to compare the outcomes of sirolimus (SRL) combined with tacrolimus (TAC) and mycophenolate mofetil (MMF) combined with TAC in kidney transplantation recipients. METHODS:A literature search of PubMed, Embase, and Web of Science was performed to identify relevant studies, and the last upd...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.transproceed.2018.08.056

    authors： Gao L,Xu F,Cheng H,Liu J

    更新日期：2018-12-01 00:00:00

  • Effects of tacrolimus on antioxidant status and oxidative stress in glioma cells.

    abstract：OBJECTIVES:After organ transplantation, some patients suffer from mild neurologic symptoms, ranging from tremor to severe complications, including seizures and encephalopathy. Among the immunosuppressants, tacrolimus can cause neurologic side effects. However, the mechanisms of encephalopathy by tacrolimus are not full...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.08.006

    authors： Jin KB,Hwang EA,Han SY,Park SB,Kim HC,Ha EY,Suh SI,Mun KC

    更新日期：2008-10-01 00:00:00

  • Long-term recurrence-free survival after liver transplantation from an ABO-incompatible living donor for treatment of hepatocellular carcinoma exceeding Milano criteria in a patient with hepatitis B virus cirrhosis: a case report.

    abstract：:The early results of liver transplantations (OLT) in patients with advanced hepatocellular carcinoma (HCC) were poor because of frequent tumor recurrence. However, OLT has significant, theoretical advantage that it removes both the tumor and the organ that is at a risk of malignancy. The Japanese law on organ transpla...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2012.01.029

    authors： Nakamura Y,Hama K,Iwamoto H,Yokoyama T,Kihara Y,Konno O,Jojima Y,Shimazu M

    更新日期：2012-03-01 00:00:00

  • In the search of potential human islet stem cells: is tetranectin showing us the way?

    abstract：:Pancreatic islet cell transplantation is a promising approach to restore the required mass of functional beta cells in diabetic patients as a means to achieve long-term normoglycemia. This therapy is, however, not yet widely used, in part because of the shortage of human islet cells. Gaining detailed knowledge of the ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2004.12.050

    authors： Hermann M,Pirkebner D,Draxl A,Margreiter R,Hengster P

    更新日期：2005-03-01 00:00:00

  • Chronic rejection: risk factors, regulation, and possible sites of therapeutic intervention.

    abstract：:Clinical chronic rejection corresponds histologically to proliferative allograft vasculopathy. This disorder reflects the cumulative trauma to the allograft regardless of its origin. As a consequence of trauma, inflammation is generated, leading to endothelial and smooth muscle cell activation, smooth muscle replicati...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,评审

    doi：10.1016/s0041-1345(98)00670-8

    authors： Häyry P

    更新日期：1998-08-01 00:00:00

  • Prevalence of polyomavirus among United Arab Emirates kidney transplant recipients: results from a single center.

    abstract：BACKGROUND:BK viremia and nephropathy are increasing problems in renal transplant recipients. The absence of a safe and effective antiviral therapy made screening-based prevention a recommended strategy. The prevalence of its reactivation among recipients of kidney transplants in the Middle East has not been well estab...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2014.11.060

    authors： Boobes Y,Bernieh B,Hussain Q,Al Omary H,Al Hakim MR,Abayechi F,El Jack H,Khan I,Ahmed M

    更新日期：2015-05-01 00:00:00

  • Initial Experience in the Use of Novel Auto-expandable Metal Ureteral Stent in the Treatment of Ureter Stenosis in Kidney Transplanted Patients.

    abstract：INTRODUCTION:Ureter stenosis in renal transplantation patients is a relatively frequent complication that negatively conditions graft evolution. The use of ureteral stents is a valid treatment alternative to the use of double-J catheters in patients for whom surgery is not contemplated or after surgical recurrence. We ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2017.09.048

    authors： Salamanca-Bustos JJ,Gomez-Gomez E,Campos-Hernández JP,Carrasco-Valiente J,Ruiz-García J,Márquez-López FJ,Zurera-Tendero L,Requena-Tapia MJ

    更新日期：2018-03-01 00:00:00

  • Experience with Alemtuzumab (Campath-1H) as induction agent in renal transplantation followed by steroid-free immunosuppression.

    abstract：BACKGROUND:The purpose of this study was to describe the initial experience with alemtuzumab as induction followed by steroid-free immunosuppression in kidney transplantation. METHODS:One hundred patients who received renal transplants from living and deceased donors were followed for a median period of 12 months (ran...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.02.026

    authors： Baez Y,Giron F,Niño-Murcia A,Rodríguez J,Salcedo S

    更新日期：2008-04-01 00:00:00

  • Systemic vascular hemodynamics and transplanted kidney survival.

    abstract：INTRODUCTION:High blood pressure and arterial stiffness contribute independently to cardiovascular mortality in uremic patients. High blood pressure is an established risk factor for chronic allograft nephropathy, recently named interstitial fibrosis/tubular atrophy (IF/TA). We sought to assess whether heart afterload ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2011.08.014

    authors： Wystrychowski G,Kolonko A,Chudek J,Zukowska-Szczechowska E,Wiecek A,Grzeszczak W

    更新日期：2011-10-01 00:00:00

  • Vitamin D deficiency in a renal transplant population: safe repletion with moderate doses of calcidiol.

    abstract：BACKGROUND:Deficits of vitamin D are a common finding in the general population, especially among patients with chronic kidney disease. However, there are not much data about its prevalence after renal transplantation. Our aim was to analyze the calcidiol status among a cohort of kidney transplant recipients, in a regi...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2010.08.015

    authors： Kanter Berga J,Crespo Albiach J,Beltran Catalan S,Gavela Martinez E,Sancho Calabuig A,Avila Bernabeu A,Pallardo Mateu LM

    更新日期：2010-10-01 00:00:00

  • The family interview in the process of donating organs and tissues for transplantation: perceptions of potential donors' relatives.

    abstract：BACKGROUND:The family interview is a complex phase of the organ donation process because it involves aspects of the interviewer, the interviewee, the interview location, and ethical and legal issues. However, there are few publications on this phase of the donation process. This study aimed to reveal the meaning assign...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2014.05.010

    authors： dos Santos MJ,Massarollo MC,de Moraes EL

    更新日期：2014-07-01 00:00:00