Abstract：
BACKGROUND:Podocyte loss contributes to the development of glomerulosclerosis. Although podocytes have been detected in the urine in certain glomerular diseases, the viability of detached cells is not known. METHODS:Urine was collected from rats with experimental membranous nephropathy [passive Heymann nephritis (PHN) model], centrifuged, and following resuspension in tissue culture media, cells were seeded onto collagen-coated tissue culture plates. Cells were grown under typical cell culture conditions. Cell number was measured, the cell type was identified by immunostaining with specific antibodies, and cell morphology was assessed by light and electron microscopy. RESULTS:Cells obtained in the urine from PHN rats were positive for synaptopodin, nephrin, podocin, WT-1, and GLEPP1 (podocyte-specific antigens). When grown ex vivo under cell culture conditions, cells obtained in the urine from PHN rats adhered to tissue culture plates, and expressed podocyte-specific proteins at the mRNA [reverse transcription-polymerase chain reaction (RT-PCR)] and protein (immunostaining) level. Cells did not stain with antibodies to mesangial (OX-7), tubular (Tamm-Horsfall protein) and endothelial (RECA) cells. Electron microscopy showed the presence of foot processes, and podocytes from PHN rats stained positive for C5b-9. Although podocyte number increased transiently during the first 5 days ex vivo, apoptosis increased significantly thereafter, reducing overall cell number. CONCLUSION:Rats with experimental membranous nephropathy shed podocytes into the urine that attach to tissue culture plates ex-vivo, and proliferate. These results suggest that detached podocytes are viable. These results add new perspectives into our understanding of podocyte loss in the development of glomerulosclerosis.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Petermann AT,Krofft R,Blonski M,Hiromura K,Vaughn M,Pichler R,Griffin S,Wada T,Pippin J,Durvasula R,Shankland SJdoi
10.1046/j.1523-1755.2003.00217.xsubject
Has Abstractpub_date
2003-10-01 00:00:00pages
1222-31issue
4eissn
0085-2538issn
1523-1755pii
S0085-2538(15)49458-Xjournal_volume
64pub_type
杂志文章abstract：:The development of donor-specific anti-human leukocyte antigen antibodies (DSAs) following renal transplantation significantly reduces long-term renal graft function and survival. The traditional therapies for antibody-mediated rejection (AMR) have provided inconsistent results and transient effects that may be due to...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2011.502
更新日期：2012-06-01 00:00:00
abstract：:The binding of albumin to the glomerular capillary wall was studied using albumin-gold in perfused kidneys, the interaction of [3H]albumin with isolated glomeruli at 37 degrees C and 4 degrees C and the interaction at [3H]albumin with purified basement membrane. The albumin-gold was found to bind predominantly to the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.149
更新日期：1995-04-01 00:00:00
abstract：:The circumstances under which furosemide increases renal blood flow was examined in mongrel dogs as it may relate to a tubuloglomerular feedback mechanism. Two maneuvers, desoxycorticosterone (DOCA) plus salt treatment and inhibition of tubular fluid flow, were used in the dogs to evaluate the renal vascular effects o...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1980.158
更新日期：1980-10-01 00:00:00
abstract：:Patients cared for in the intensive care unit (ICU) undergo multiple interventions to treat serious medical conditions. In addition to the acute illness being treated, underlying chronic conditions require ongoing drug therapy. As a result, these patients are exposed to numerous pharmaceutical agents, many of which ha...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.475
更新日期：2012-06-01 00:00:00
abstract：:Normal urine contains thousands of proteins, largely due to the presence of 'exosomes,' tiny vesicles secreted into the urine by renal epithelial cells. These exosomes, demonstrated by Keller and colleagues to be also retrievable from amniotic fluid, offer great promise for future disease biomarker discovery studies. ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5002510
更新日期：2007-11-01 00:00:00
abstract：:The candidacidal activity of kidneys, liver, and spleen's phagocytic systems was studied in mouse. Different strains of mice were inoculated intravenously (i.v.) with 1 to 2.6 x 10(4) viable Candida albicans. Elimination of the microorganisms from the kidneys, liver, and spleen were evaluated by enumeration of colony ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1991.225
更新日期：1991-09-01 00:00:00
abstract：:Urinary exosomes containing apical membrane and intracellular fluid are normally secreted into the urine from all nephron segments, and may carry protein markers of renal dysfunction and structural injury. We studied methods for collection, storage, and preservation of urinary exosomal proteins. We collected urine fro...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000273
更新日期：2006-04-01 00:00:00
abstract：BACKGROUND:Current renal substitution therapy for acute or chronic renal failure with hemodialysis or hemofiltration is life sustaining, but continues to have unacceptably high morbidity and mortality rates. This therapy is not complete renal replacement therapy because it does not provide active transport nor metaboli...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.00486.x
更新日期：1999-06-01 00:00:00
abstract：:The plasmin protease system may have a role in maintaining the patency of renal tubules and in regulating matrix degradation within the glomerulus. Urokinase-plasminogen activator (u-PA) is a serine protease which plays an important part in the regulation of plasmin production from plasminogen. The synthesis of u-PA b...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1994.5
更新日期：1994-01-01 00:00:00
abstract：:The purpose of these studies was to quantitatively assess the role of endogenous angiotensin II activity in controlling transport in the proximal convoluted tubule (PCT) and whole nephron. We used the nonpeptide angiotensin II receptor antagonist DuP 753, which lacks the agonist and kinin/prostaglandin-inducing proper...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.228
更新日期：1990-09-01 00:00:00
abstract：:The cytokine interleukin-6 (IL-6) is a major cell regulatory factor that may play an important role in the bone remodeling of patients with renal failure. IL-6 exerts its action by binding to its receptor (IL-6R), which leads to transduction of a second messenger cascade within cells. In vitro as well as in vivo data ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.343
更新日期：1996-08-01 00:00:00
abstract：:The purpose of these studies was to test the hypothesis that Randall's plaque develops in unique anatomical sites of the kidney and their formation is conditioned by specific stone-forming pathophysiologies. We performed intraoperative papillary biopsies from kidneys of idiopathic-calcium oxalate (CaOx), intestinal by...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5000238
更新日期：2006-04-01 00:00:00
abstract：:T lymphocytes modulate early ischemia-reperfusion injury in the kidney; however, their role during repair is unknown. We studied the role of TCRbeta(+)CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs), known to blunt immune responses, in repair after ischemia-reperfusion injury to the kidney. Using a murine model of is...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.259
更新日期：2009-10-01 00:00:00
abstract：:The increasing use of mice in renal and cardiovascular studies has necessitated adapting physiological methods used for rats to mice, which are far smaller in size. We have adapted measurements of continuous renal blood flow, pressure natriuresis and diuresis, and laser-Doppler cortical and medullary flow to 40 g mice...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1997.462
更新日期：1997-11-01 00:00:00
abstract：:In membranous nephropathy (MN) overproduction of matrix by glomerular epithelial cells (GEC) is believed to be responsible for glomerular basement membrane thickening and spikes. We studied experimental MN in rats (passive Heymann nephritis, PHN) at 5, 10 and 30 days. PHN rats exhibited a marked increase in GEC immuno...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.294
更新日期：1996-07-01 00:00:00
abstract：:Lysophosphatidic acid (LPA) is known to regulate various biological responses by binding to LPA receptors. The serum level of LPA is elevated in diabetes, but the involvement of LPA in the development of diabetes and its complications remains unknown. Therefore, we studied LPA signaling in diabetic nephropathy and the...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.11.010
更新日期：2017-06-01 00:00:00
abstract：:The present study was undertaken to: (a) clarify the comparative renal potency of bolus injection of the natriuretic peptides urodilatin and ANF99-126 in the rat; (b) establish whether or not intravenous (i.v.) infusion of urodilatin (200 ng/min) combined with dopamine (UD) to maintain mean arterial pressure could imp...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.399
更新日期：1992-11-01 00:00:00
abstract：:Hemodynamic studies were performed at two levels of dietary sodium intake (10 mmoles/day and 100 mmoles/day for 14 days) in a group of patients (with retained kidneys) on chronic hemodialysis. Plasma renin activity and aldosterone, plasma, and extracellular fluid (ECF) volumes were measured while the patient were at r...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1977.114
更新日期：1977-10-01 00:00:00
abstract：:Angiotensin converting enzyme (ACE) inhibitors are known to lower urinary protein excretion in human renal disease. This proteinuria lowering effect of ACE inhibition has been hypothesized to be a result of renal hemodynamic changes due to the inhibition of angiotensin II (Ang II) production. To test this hypothesis w...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1991.268
更新日期：1991-10-01 00:00:00
abstract：BACKGROUND:Macrophages have been regarded as "blackguards" in the generation of glomerular injury. However, it is still unclear what kind of cellular machinery is responsible for their pathogenic actions. To explore this issue, this investigation aims at developing a novel strategy using adoptive transfer of "loss-of-f...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00893.x
更新日期：2000-02-01 00:00:00
abstract：:Hyperlipidemia can induce or aggravate renal tubulointerstitial injury. Experiments in a complex rat model with chronic glomerulonephritis and long-standing, coexisting hyperlipidemia suggested that induction of xanthine oxidase (XO), with increased oxygen radical generation, is involved in aggravation of tubulointers...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000121
更新日期：2006-02-01 00:00:00
abstract：:Cilnidipine, a dual L-/N-type calcium channel blocker, dilates both efferent and afferent arterioles and is renoprotective. Our multi-center, open-labeled, and randomized trial compared the antiproteinuric effect of cilnidipine with that of amlodipine in hypertensive patients with kidney disease. A group of 339 patien...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.ki.5002623
更新日期：2007-12-01 00:00:00
abstract：:Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.166
更新日期：1988-08-01 00:00:00
abstract：:IgG4-related disease is a recently recognized multi-organ disorder characterized by high levels of serum IgG4 and dense infiltration of IgG4-positive cells into several organs. Although the pancreas was the first organ recognized to be affected by IgG4-related disorder in the syndrome of autoimmune pancreatitis, we pr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.271
更新日期：2010-11-01 00:00:00
abstract：BACKGROUND:The hypothesis that angiotensin-converting enzyme (ACE) inhibitors interfere with the treatment of anemia with recombinant human erythropoietin (rHuEPO) remains controversial. To test this hypothesis, a retrospective analysis was conducted in a large group of hemodialysis patients with renal anemia. METHODS...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00028.x
更新日期：2001-11-01 00:00:00
abstract：:The structure of the duodenal mucosa was evaluated in duodenal biopsy samples obtained from patients with moderate renal failure (MRF) and in dialysis patients (HD) in an effort to examine the possibility that changes in duodenal mucosa may contribute to the impaired calcium absorption in renal failure (RF). The effec...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1981.23
更新日期：1981-02-01 00:00:00
abstract：BACKGROUND:Epidermal growth factor (EGF) plays an important role in renal tubular regeneration after ischemic injury in kidney. The present study reports the association between the renin-angiotensin system (RAS) and EGF, and the effect of angiotensin II blockade with losartan (LSRT) on EGF expression in an experimenta...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.060003847.x
更新日期：2001-09-01 00:00:00
abstract：:A model of glomerular dynamics has been developed by using network thermodynamics and the SPICE 2 computer program to further explore the determinants of glomerular filtration. The model is designed to be holistic and self-adjusting, taking cognizance of and permitting quantitation of the secondary alterations in indi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1981.27
更新日期：1981-02-01 00:00:00
abstract：:Recent decades have witnessed the revelation of expanding roles of the vitamin D endocrine system beyond calcium and phosphorus metabolism. Along with these non-calcemic or non-classic actions of vitamin D are newly discovered therapeutic actions of vitamin D analogs in a number of pathological conditions, including k...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.175
更新日期：2010-07-01 00:00:00
abstract：:Anemia can contribute to chronic allograft injury by limiting oxygen delivery to tissues, particularly in the tubulointerstitium. To determine mechanisms by which erythropoietin (EPO) prevents chronic allograft injury we utilized a rat model of full MHC-mismatched kidney transplantation (Wistar Furth donor and Lewis r...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.473
更新日期：2012-05-01 00:00:00
