Abstract：
:We have studied the cellular expression and functional conservation of the human La autoantigen across species by introducing genes encoding human La antigen into cultured murine cell lines. In transfected murine fibroblasts and lymphoid cell lines human La was expressed as a predominantly nuclear antigen, with a typical pattern of nuclear speckling. Radiolabeled human La was of the predicted molecular mass (48 kDa) when expressed in murine cells and was associated intracellularly with murine 60 kDa Ro antigen. Under certain conditions of cell lysis the association between human La and murine Ro was disrupted, whereas human La and human Ro complexes remained intact, suggesting the possibility of species-specific protein interaction between La/Ro antigens. Expression of human La from the genomic gene construct was associated in some transfectants with the presence of additional high molecular weight bands reactive with anti-La monoclonal antibodies. Cell surface La determinants were not detected on any of the transfected cells. Human La protein associated with many of the same radiolabeled RNAs as the murine La antigen in transfected murine cells, indicating intact RNA-binding function of La protein across species. The findings indicate that despite 23% non-identity within the primary structure of human and murine La, the human molecule is functionally highly conserved across species.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Keech CL,Gordon TP,Reynolds P,McCluskey Jdoi
10.1006/jaut.1993.1045subject
Has Abstractpub_date
1993-10-01 00:00:00pages
543-55issue
5eissn
0896-8411issn
1095-9157pii
S0896-8411(83)71045-0journal_volume
6pub_type
杂志文章abstract：:Costimulatory molecules are cell-surface glycoproteins that can direct, modulate and fine tune immune responses. B7-2(CD86) costimulatory molecules are considered as major regulators of T cell responses, acting by appropriate interactions with the stimulatory CD28 or inhibitory CTLA-4 receptors found on T cells. Altho...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2010.06.006
更新日期：2010-11-01 00:00:00
abstract：:The development of insulin dependent diabetes mellitus (IDDM) and diabetes in the diabetes prone (DP) BB rat animal model of IDDM is thought to be due to an autoimmune process. Natural killer (NK) cells have been implicated but not proven to play a pathogenetic role in BB rats due to the increased NK cell number and a...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/s0896-8411(95)80021-2
更新日期：1995-12-01 00:00:00
abstract：:Our aim was to investigate presence of tumour necrosis factor (TNF) and interleukin (IL)-10 in serum and their relation to different genotypes as well as to clinical and laboratory phenotypes in patients with polymyositis and dermatomyositis. In 65 patients with poly- or dermatomyositis the inflammatory cytokine balan...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2006.06.003
更新日期：2006-08-01 00:00:00
abstract：:GAD65 (glutamic acid decarboxylase) is an important autoantigen in both type 1 (insulin-dependent) diabetes mellitus (IDDM) and the neurological autoimmune disease stiff-man syndrome (SMS), and is expressed in pancreatic islets as well as the nervous system. Still, only 30% of SMS patients also have type 1 diabetes. T...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1999.0280
更新日期：1999-06-01 00:00:00
abstract：:Nasal tolerance has recently been used to modulate immune responses in animal models of autoimmunity. We have compared immunogenic collagen type II (CII) peptides for induction of nasal tolerance in DBA/1 mice to collagen-induced arthritis (CIA). Three synthetic peptides corresponding to T cell-stimulating sequences o...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1998.0255
更新日期：1999-02-01 00:00:00
abstract：:Exposure to the hydrocarbon oil pristane induces lupus specific autoantibodies in non-autoimmune mice. We investigated whether the capacity to induce lupus-like autoimmunity is a unique property of pristane or is shared by other adjuvant oils. Seven groups of 3-month-old female BALB/cJ mice received a single intraperi...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/s0896-8411(03)00083-0
更新日期：2003-08-01 00:00:00
abstract：:Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and characterized five human monoclonal antibodies. These antibodies were derived by fusing a regional hepatic lymph node from a patient with PBC with the mouse human heterohybrid cell line F3B6. Previ...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/0896-8411(92)90187-u
更新日期：1992-12-01 00:00:00
abstract：:Epidemiology is expected to provide important clues to our understanding of the enigmatic etiopathogenesis of primary biliary cirrhosis (PBC). First, a systematic review of population based studies indicated a wide range in the yearly incidence (0.33-5.8/100.000) and point prevalence (1.91-40.2/100.000) rates. Though ...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1016/j.jaut.2013.06.015
更新日期：2013-10-01 00:00:00
abstract：:Many years of work, multiplex family collection and endless genotyping finally give fruit. The original aim cannot be lost. The aim is not only to identify mutations involved in susceptibility for systemic lupus erythematosus (SLE) but to elucidate the disease pathogenesis as well. After genetics comes the biology. We...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1016/s0896-8411(03)00093-3
更新日期：2003-09-01 00:00:00
abstract：:Improved clinical findings of inflammatory bowel disease (IBD) upon treatment with helminthes and their ova were proven in animal models of IBD and in human clinical studies. The immunomodulatory properties of several helminthes were attributed to the phosphorylcholine (PC) molecule. We assessed the therapeutic potent...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2014.11.001
更新日期：2015-01-01 00:00:00
abstract：:Vitiligo is an acquired dermatological disorder characterized by a loss of epidermal melanocytes resulting in depigmentation of the skin. Mechanisms underlying the destruction of melanocytes in vitiligo remain unclear. An animal model to study spontaneously occurring autoimmune vitiligo is the mutant Smyth line (SL) o...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2003.09.006
更新日期：2004-02-01 00:00:00
abstract：:Epitopes with linear sequences recognized by anti-La autoantibodies from seven mothers of children with congenital heart block were recently defined. Eight of these epitopes share sequence identity with three other proteins in addition to the original autoantigen, La. The three proteins are human cardiac myosin beta h...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/0896-8411(92)90007-d
更新日期：1992-08-01 00:00:00
abstract：:University of California at Davis (UCD) lines 200 and 206 chickens develop a hereditary systemic scleroderma-like connective tissue disease characterized by severe lymphocytic infiltration and excessive accumulation of collagen in skin and internal organs. The immune system seems to play an important role in the devel...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1995.0057
更新日期：1995-10-01 00:00:00
abstract：:Class II antigens of the major histocompatibility complex (MHC) have a well-defined role in restricting cellular interactions and presenting processed antigen to T cells. In addition, a fundamental role for Class II antigens in cellular activation has been suggested, following studies demonstrating that Class II antig...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/0896-8411(89)90133-9
更新日期：1989-06-01 00:00:00
abstract：:Malathion administration at non-cholinergical doses was shown to elevate macrophage, proliferative and humoral immune responses. This study examined the effects of malathion on autoimmunity, autoantibody formation, macrophage function and mitogenic responses in MRL-lpr mice (genetically predisposed to autoimmune disea...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1997.0145
更新日期：1997-08-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease primarily afflicting women. The reason for the gender bias is unclear, but genetic susceptibility, estrogen and environmental agents appear to play significant roles in SLE pathogenesis. Environmental agents can contribute to lupus susceptibility through epig...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2011.11.001
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:Antiphospholipid syndrome (APS) is an acquired thrombophilia characterized by recurrent thrombosis and/or pregnancy morbidity, in the presence of antibodies to β2 glycoprotein-I (β2GPI), prothrombin or Lupus anticoagulant (LA). Anti-β2GPI antibodies recognize complexes of β2GPI dimers with CXCL4 chemokine and...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2020.102527
更新日期：2020-12-01 00:00:00
abstract：:To characterize the humoral immune response to the protein tyrosine phosphatase (PTP)-like autoantigen (IA-2) in preclinical type 1 diabetes (T1D), and to assess the utility of epitope and isotype-specific IA-2 antibody responses as surrogate markers for disease development, we analyzed these antibodies in 34 initiall...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2004.09.005
更新日期：2004-12-01 00:00:00
abstract：:Promiscuous expression of a plethora of tissue-restricted antigens (TRAs) in medullary thymic epithelial cells (mTECs) is essential for central tolerance. This promiscuous gene expression (pGE) is characterized by inclusion of a broad range of TRAs and by its mosaic expression patterns, i.e. each antigen is only expre...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2015.10.001
更新日期：2016-02-01 00:00:00
abstract：:The thymus of New Zealand black (NZB) mice undergoes premature involution. In addition, cultured thymic epithelial cells from NZB mice undergo accelerated preprogrammed degeneration. NZB mice also have distinctive and well-defined abnormalities of thymic architecture involving stromal cells, defined by staining with m...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1999.0325
更新日期：1999-11-01 00:00:00
abstract：:Helper T cells are required for development of the autoantibody responses to native mouse erythrocytes (MRBC) that spontaneously develop in NZB mice. However, the stimulus for these Th is not known. Therefore, we compared the abilities of splenic T cells from actively autoimmune old NZB mice and preautoimmune, young N...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1996.0062
更新日期：1996-08-01 00:00:00
abstract：:The higher concordant occurrence of autoimmune diseases in monozygotic twins compared to dizygotic or sibling pairs supports the role for genetic susceptibility. For most conditions, however, concordance rates are considerably below 100% and lead to the estimate of the weight of genetics coined "heritability". In the ...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1016/j.jaut.2012.07.011
更新日期：2012-12-01 00:00:00
abstract：:The obstetric management of women with antiphospholipid (aPL) syndrome remains controversial. Despite recent advances, the controversies have been fueled by our limited understanding of the multi-factorial causes of aPL-associated pregnancy loss and the lack of data from randomized studies. We have escaped from the na...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1006/jaut.2000.0417
更新日期：2000-09-01 00:00:00
abstract：:Ultraviolet (UV) light is a known trigger of skin and possibly systemic inflammation in systemic lupus erythematosus (SLE) patients. Although type I interferons (IFN) are upregulated in SLE skin after UV exposure, the mechanisms to explain increased UVB-induced inflammation remain unclear. This paper compares the role...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2019.06.002
更新日期：2019-09-01 00:00:00
abstract：:IDDM2-encoded predisposition to type 1 diabetes has recently been mapped to the minisatellite or variable number of tandem repeat (VNTR) locus upstream of the insulin and insulin-like growth factor II genes on human chromosome 11p15.5. In a UK case-control study (n = 228 sporadic diabetics; n = 441 healthy controls), ...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1996.0057
更新日期：1996-06-01 00:00:00
abstract：:Autoinflammatory diseases (AUIDs) are a genetically heterogeneous group of rheumatic diseases characterized by episodic inflammation linked with dysregulated innate immune responses. In this review, we summarize the molecular mechanisms altered by disease-associated variants in several AUIDs, including NOD2-associated...
journal_title：Journal of autoimmunity
pub_type： 杂志文章,评审
doi：10.1016/j.jaut.2017.10.012
更新日期：2018-03-01 00:00:00
abstract：:Chronic myocarditis often progresses to dilated cardiomyopathy resulting in heart failure or cardiac transplantation. Viral infection is the most common cause of myocarditis and coxsackie B viruses (CBV) are the most frequently cited etiologic agents associated with myocarditis and cardiomyopathy. Additionally, CBV in...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2005.05.007
更新日期：2005-09-01 00:00:00
abstract：:We have previously described an SLE-derived human hybridoma autoantibody, 9604, which binds to activated but not to resting platelets, inhibits platelet aggregation, and immunoprecipitates a surface-labelled poly-peptide of 32,000 molecular weight (MW) (p32). In the present study, using a murine monoclonal anti-p32 an...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1006/jaut.1995.0008
更新日期：1995-02-01 00:00:00
abstract：:Glutamic acid decarboxylase 65 (GAD65) is a putative autoantigen associated with the pathogenesis of type 1 diabetes (T1D). The prevalence of autoreactive CD4+ T cells towards the immunodominant GAD65(555-567) epitope in DR4 healthy and T1D subjects was investigated with class II tetramers. A slightly higher percentag...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2005.08.007
更新日期：2005-12-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is a complex autoimmune disease with a poorly understood preclinical stage of immune dysregulation and symptom accrual. Accumulation of antinuclear autoantibody (ANA) specificities is a hallmark of impending clinical disease. Yet, many ANA-positive individuals remain healthy, suggest...
journal_title：Journal of autoimmunity
pub_type： 杂志文章
doi：10.1016/j.jaut.2016.06.001
更新日期：2016-11-01 00:00:00
