Abstract:
OBJECTIVE:Antiphospholipid syndrome (APS) is an acquired thrombophilia characterized by recurrent thrombosis and/or pregnancy morbidity, in the presence of antibodies to β2 glycoprotein-I (β2GPI), prothrombin or Lupus anticoagulant (LA). Anti-β2GPI antibodies recognize complexes of β2GPI dimers with CXCL4 chemokine and activate platelets. Thrombospondin 1 (TSP-1) is secreted by platelets and exhibits prothrombotic and proinflammatory properties. Therefore, we investigated its implication in APS. METHODS:Plasma from APS patients (n = 100), Systemic Lupus Erythematosus (SLE) (n = 27) and healthy donors (HD) (n = 50) was analyzed for TSP-1, IL-1β, IL-17A and free active TGF-β1 by ELISA. Human Umbilical Vein Endothelial Cells (HUVECs) and HD monocytes were treated with total HD-IgG or anti-β2GPI, β2GPI and CXCL4 and CD4+ T-cells were stimulated by monocyte supernatants. TSP-1, IL-1β, IL-17A TGF-β1 levels were quantified by ELISA and Real-Time PCR. RESULTS:Higher plasma levels of TSP-1 and TGF-β1, which positively correlated each other, were observed in APS but not HDs or SLE patients. Patients with arterial thrombotic events or those undergoing a clinical event had the highest TSP-1 levels. These patients also had detectable IL-1β, IL-17A in their plasma. HD-derived monocytes and HUVECs stimulated with anti-β2GPI-IgG-β2GPI-CXCL4 secreted the highest TSP-1 and IL-1β levels. Supernatants from anti-β2GPI-β2GPI-CXCL4 treated monocytes induced IL-17A expression from CD4+ T-cells. Transcript levels followed a similar pattern. CONCLUSIONS:TSP-1 is probably implicated in the pathogenesis of APS. In vitro cell treatments along with high TSP-1 levels in plasma of APS patients suggest that high TSP-1 levels could mark a prothrombotic state and an underlying inflammatory process.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Patsouras M,Tsiki E,Karagianni P,Vlachoyiannopoulos PGdoi
10.1016/j.jaut.2020.102527subject
Has Abstractpub_date
2020-12-01 00:00:00pages
102527eissn
0896-8411issn
1095-9157pii
S0896-8411(20)30151-7journal_volume
115pub_type
杂志文章abstract::With unknown etiology, scleroderma (SSc) is a multifaceted disease characterized by immune activation, vascular complications, and excessive fibrosis in internal organs. Genetic studies, including candidate gene association studies, genome-wide association studies, and whole-exome sequencing have supported the notion ...
journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,多中心研究
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journal_title:Journal of autoimmunity
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.2001.0545
更新日期:2001-12-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/0896-8411(88)90078-9
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,评审
doi:10.1016/0896-8411(92)90015-i
更新日期:1992-04-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1006/jaut.1995.0057
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2009.10.003
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/0896-8411(91)90055-h
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/0896-8411(95)90008-x
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/0896-8411(92)90007-d
更新日期:1992-08-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:Journal of autoimmunity
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.jaut.2016.11.004
更新日期:2017-01-01 00:00:00
abstract::Antiphospholipid syndrome (APS) is characterized by thrombosis and pregnancy loss in the presence of antiphospholipid antibodies (aPL), mainly anticardiolipin, anti-beta2-glycoprotein I and lupus anticoagulant. However, similar to systemic lupus erythematosus, APS is also characterized by multiple other autoantibodies...
journal_title:Journal of autoimmunity
pub_type: 杂志文章
doi:10.1016/j.jaut.2007.11.011
更新日期:2008-02-01 00:00:00