The efficiency of an autotransfusion system for tumor cell removal from blood salvaged during cancer surgery.

Abstract:

:The efficiency of a gravity-dependent autotransfusion system (ATS) for filtering tumor cells from blood was examined under laboratory and clinical conditions. In laboratory studies, reconstituted whole blood was seeded with known numbers of cultured A431 human vulva carcinoma cells then filtered through the ATS. Cancer cells were recovered from blood samples taken before and after filtration using a density gradient centrifugation procedure, and identified by immunocytochemical techniques, based upon their cytoskeletal intermediate filament proteins. It was estimated that 55%-76% of the A431 cells were retained by the ATS filters, and of those cancer cells that passed through the ATS, 62% suffered lethal trauma and the remainder showed morphologic damage. In clinical studies, blood was harvested from the surgical site during resection of primary cancers from three patients. In agreement with the laboratory studies, 50%-68% of tumor cells were filtered by the ATS from blood harvested during surgery. These results are discussed in terms of metastatic inefficiency and the potential for use of autotransfused blood salvaged during cancer surgery.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Karczewski DM,Lema MJ,Glaves D

doi

10.1213/00000539-199406000-00017

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

1131-5

issue

6

eissn

0003-2999

issn

1526-7598

journal_volume

78

pub_type

杂志文章
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    doi:

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  • Dexmedetomidine, an alpha 2-adrenergic agonist, decreases cerebral blood flow in the isoflurane-anesthetized dog.

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  • Resuscitation of Endotheliopathy and Bleeding in Thoracic Aortic Dissections: The VIPER-OCTA Randomized Clinical Pilot Trial.

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    journal_title:Anesthesia and analgesia

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  • Anesthesia for Percutaneous Pulmonary Valve Implantation: A Case Series.

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    更新日期:2018-07-01 00:00:00

  • Anesthetic and hemodynamic effects of single bolus versus incremental titration of hyperbaric spinal lidocaine through microcatheter.

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    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验

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    journal_title:Anesthesia and analgesia

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    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章

    doi:10.1097/00000539-199611000-00022

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  • Epidurals in patients receiving thromboprophylaxis with unfractionated heparin three times a day: the value of activated partial thromboplastin time testing.

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    journal_title:Anesthesia and analgesia

    pub_type: 杂志文章

    doi:10.1213/00000539-199402000-00014

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    更新日期:1994-02-01 00:00:00

  • Testing anal sphincter tone predicts the effectiveness of caudal analgesia in children.

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    更新日期:2002-05-01 00:00:00

  • The effect and duration of prophylactic platelet transfusions before insertion of a central venous catheter in patients with bone marrow failure evaluated with point-of-care methods and flow cytometry.

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    更新日期:2014-10-01 00:00:00

  • The effects of milrinone on platelets in patients undergoing cardiac surgery.

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    journal_title:Anesthesia and analgesia

    pub_type: 临床试验,杂志文章,随机对照试验

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  • The chemical stability and sterility of sodium thiopental after preparation.

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    journal_title:Anesthesia and analgesia

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    doi:10.1097/00000539-199801000-00040

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  • Verifying correct endotracheal intubation by measurement of end-tidal carbon dioxide during an ex utero intrapartum treatment procedure.

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    doi:10.1213/00000539-199105000-00010

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  • Residual neuromuscular block: lessons unlearned. Part I: definitions, incidence, and adverse physiologic effects of residual neuromuscular block.

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    pub_type: 杂志文章,评审

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  • Supraventricular arrhythmias in intensive care unit patients: short and long-term consequences.

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    pub_type: 杂志文章,meta分析,评审

    doi:10.1213/ANE.0000000000000914

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  • The volatile anesthetic isoflurane inhibits the histamine-induced Ca2+ influx in primary human endothelial cells.

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  • Exhaled Propofol Concentrations Correlate With Plasma and Brain Tissue Concentrations in Rats.

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