Abstract:
:Hypothyroid patients and mice have been shown to have circulating TSH that is more highly sialylated than their euthyroid counterparts. To learn about the underlying cellular mechanisms responsible for this increased sialylation of TSH, we used in situ hybridization to examine the beta-galactoside alpha-2,6-sialyltransferase (STase) mRNA content in thyrotrophs and corticotrophs of euthyroid and hypothyroid mice. Mice were treated with or without 0.05% propylthiouracil for 1, 2, 3, 4, or 6 weeks, then pituitaries were removed, and 5-microns slices were immunocytochemically stained for TSH and ACTH. Adjacent sections were used for in situ hybridization. A 48-mer deoxynucleotide probe to rat STase and two control probes were labeled with 35S, and autoradiography was performed. There was an approximately 140% increase in STase mRNA in hypothyroid thyrotrophs compared to euthyroid thyrotrophs by the first week, with a mean increase of 170% in weeks 1-6, whereas corticotrophs exhibited no change in STase mRNA. The increase in hybridization of the STase probe in hypothyroid thyrotrophs may be due to an increased transcription of the STase gene, stabilization of the STase mRNA, or both. Thus, modulation of the STase mRNA levels occurs in thyrotrophs and represents one important mechanism by which the oligosaccharides of TSH are altered under different physiological conditions.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Helton TE,Magner JAdoi
10.1210/endo.134.6.8194460subject
Has Abstractpub_date
1994-06-01 00:00:00pages
2347-53issue
6eissn
0013-7227issn
1945-7170journal_volume
134pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Folliculostellate (FS) cells are known to communicate with each other and with endocrine cells via gap junctions in the anterior pituitary. We investigated whether TGFbeta3 and estradiol, known to regulate FS cell production and secretion of basic fibroblast growth factor (bFGF), increases gap junctional communication...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0122
更新日期:2005-09-01 00:00:00
abstract::Recent evidence indicates that catecholamines may directly alter anterior pituitary function. In the present study, an in vitro perifusion system was used to investigate whether catecholamines affect the gonadotrope. Pituitary tissue from castrated ram lambs was incubated in the presence of epinephrine (EPI), norepine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2425
更新日期:1986-06-01 00:00:00
abstract::Although viral infection is thought to be associated with subacute thyroiditis and probably with autoimmune thyroid disease, possible changes in thyroid function during the prodromal period of infection or subclinical infection remain largely unknown. Recently, it was shown that pathogen-associated molecular patterns ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1638
更新日期:2007-07-01 00:00:00
abstract::Native PRL and des-amido forms 1, 2, and 3 were tested for their individual immunochemical and receptor-binding abilities. The results show that deamidation of either secreted mouse PRL or stored ovine PRL alters their binding in a radioreceptor assay. For each accumulated deamidation there was a statistically signifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-353
更新日期:1985-01-01 00:00:00
abstract::Throughout spermatogenesis, germ cells migrate from the basal to the adluminal compartment while remaining attached to Sertoli cells via actin-based adherens and intermediate filament-based anchoring junctions. However, the events that trigger deadhesion and adhesion remain largely unknown. As part of our continued ef...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220893
更新日期:2003-04-01 00:00:00
abstract::Previously, a human CYP3A4-transgenic (Tg-CYP3A4) mouse line was reported to exhibit enhanced metabolism of midazolam by cytochrome P450 3A4 (CYP3A4) expressed in small intestine. Here we show that expression of CYP3A4 and murine cyp3a and cyp2b was both age and sex dependent. CYP3A4 was expressed in the livers of mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1248
更新日期:2005-07-01 00:00:00
abstract::LH receptor (LHR) expression in the ovary is regulated by the RNA binding protein, (LHR mRNA binding protein [LRBP]), which has been identified as being mevalonate kinase. This study examined the role of microRNA miR-122 in LRBP-mediated LHR mRNA expression. Real-time PCR analysis of ovaries from pregnant mare serum g...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1619
更新日期:2013-12-01 00:00:00
abstract::Thyroid hormones (TH) are actively taken up into target cells via TH-transmembrane transporters (THTT). Their activity and expression patterns define a layer of endocrine regulation that is poorly understood. Therefore, THTT are potential targets for interfering agents (endocrine disruptors) as well as for pharmacolog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1016
更新日期:2015-07-01 00:00:00
abstract::To investigate the mechanism of insulin insensitivity during fasting and hyperresponsiveness to insulin after refeeding, insulin binding, 2-deoxyglucose uptake and glucose oxidation were measured in adipocytes from three groups of rats: control, 48 h fasted, and 48 h refed after 48 h fasting. In 48 h fasted rats, fat ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-5-1384
更新日期:1977-05-01 00:00:00
abstract::1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] inhibits proliferation of normal and malignant prostate epithelial cells at least in part through inhibition of G1 to S phase cell cycle progression. The mechanisms of the antiproliferative effects of 1,25-(OH)2D3 have yet to be fully elucidated but are known to require the vita...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0434
更新日期:2010-10-01 00:00:00
abstract::Although mothers against dpp (MAD) and its related proteins (MADR) are believed to be important components of the cell signaling pathway for the transforming growth factor beta (TGFbeta) superfamily, the presence and regulation of these signaling molecules in ovarian cells by TGFbeta is not known. In the present studi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5376
更新日期:1997-09-01 00:00:00
abstract::In the rat ovary, follicle cells produce and respond to activin, but as yet, the functional significance of the autocrine/paracrine effects of ovarian activin remains equivocal. To assess the effects of activin on folliculogenesis, normal cycling female rats were injected once every 8 h over a 40-h period with recombi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.11.7588210
更新日期:1995-11-01 00:00:00
abstract::Mammary gland explants from mature virgin mice, rats, and rabbits exhibit an increased rate of both DNA and casein syntheses when cultured in the presence of specific combinations of insulin, hydrocortisone, and PRL. If cytosine arabinoside, a potent inhibitor of DNA synthesis, is added to the culture medium, casein s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-988
更新日期:1983-03-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1), in the form of either GLP-1-(7-36)amide or GLP-1-(7-37), has been shown to potently stimulate insulin release in a glucose-dependent manner and is suggested to be a physiological incretin. To explore the mechanisms by which GLP-1-(7-36)amide stimulates insulin release, we investigated ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404610
更新日期:1993-10-01 00:00:00
abstract::The effect of conditioned vs. fresh culture medium on the dopaminergic inhibition of TSH and PRL secretion by primary cultures of male rat anterior pituitary cells has been studied. In the presence of conditioned medium (that had been in contact with the cells over the 3-day culture period) 10(-6) M dopamine (DA) inhi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1319
更新日期:1986-04-01 00:00:00
abstract::Over 6 billion pounds per year of the estrogenic monomer bisphenol A (BPA) are used to manufacture polycarbonate plastic products, in resins lining metal cans, in dental sealants, and in blends with other types of plastic products. The ester bond linking BPA molecules in polycarbonate and resins undergoes hydrolysis, ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2005-1159
更新日期:2006-06-01 00:00:00
abstract::Although estrogens are neuroprotective in young adult animal models of stroke, clinical trials demonstrate that estrogens increase the incidence and severity of stroke in aged women. We have previously shown that experimental stroke pathophysiology differs between young adult and aged rats. The aim of this study was t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1859
更新日期:2012-07-01 00:00:00
abstract::To examine the relationship between the expression of insulin-like growth factor (IGF)-binding protein-2 (IGFBP-2) and cell growth in a cell type with a defined IGF/IGFBP system, an ovine IGFBP-2 complementary DNA was overexpressed in C6 glioma cells. C6 cells produce IGFBP-3, IGFBP-4, a negligible amount of IGFBP-2, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6498
更新日期:1999-02-01 00:00:00
abstract::The utility of the established ACTH secreting mouse pituitary tumor cell line AtT20 for investigating early glucocorticoid inhibition was examined. Three different strains of the cell line D1, D16v, and D16:16, respectively, were analyzed. In initial studies CRF and phorbol esters were used as secretagogues to examine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1332850
更新日期:1992-12-01 00:00:00
abstract::The effect of active and passive immunization with luteinizing hormone-releasing hormone (LHRH) on serum LH and follicle-stimulating hormone (FSH) levels and the ultrastructure of the pituitary gonadotrophs was investigated in castrated male rats. Two weeks after castration, the animals were immunized with Glu1-LHRH c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-1-291
更新日期:1976-07-01 00:00:00
abstract::This study was designed to test the hypothesis that systemic immune challenge with endotoxin inhibits the reproductive axis centrally by suppressing GnRH pulsatile release into hypophyseal portal blood. Using alert, normally behaving, ovariectomized ewes, we sampled hypophyseal portal blood at 10-min intervals beginni...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5449
更新日期:1997-10-01 00:00:00
abstract::In mice, GH levels rise in response to short-term fasting or starvation (food restriction to 40% of ad libitum intake), similar to that which occurs in humans in response to fasting or anorexia. Recent studies using acyl-ghrelin knockout mice have suggested that the rise in GH during food restriction is essential to s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1849
更新日期:2013-01-01 00:00:00
abstract::The preoptic area (POA) of the male dove is a known target area for separable behavioral actions of testosterone and 17 beta-estradiol (E2) and contains an active aromatase system. We have examined the regulatory influence of gonadal hormones on aromatase using an in vitro microassay that measures conversion of [1 alp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2180
更新日期:1986-06-01 00:00:00
abstract::Brown adipose tissue (BAT) thermogenesis is activated by the sympathetic nervous system. BAT responses to norepinephrine are blunted in hypothyroidism and are rapidly restored by thyroid hormone. We examined in rats the effects of thyroid hormone on BAT beta 1- and beta 2-adrenergic receptors (AR) expression and capac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628360
更新日期:1995-08-01 00:00:00
abstract::Osteoclasts are bone-resorbing cells whose normal function depends in part upon their ability to migrate over the bone surface to initiate new sites of bone resorption. The growth factor/cytokine, colony-stimulating factor-1 (CSF-1), potently stimulates osteoclast motility, in a c-src-dependent fashion. The intracellu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.6.7480
更新日期:2000-06-01 00:00:00
abstract::Mice with the recessively inherited obese-hyperglycemic syndrome (ob/ob) and their nonobese litter mates were studied over a 26-week period. The body weights and serum glucose levels of ob/ob mice began to rise markedly at 5-6 weeks of age and remained elevated throughout the period of study. Obese mice were significa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-1-139
更新日期:1976-01-01 00:00:00
abstract::The activity of many endocrine cells is regulated by gamma-aminobutyric acid (GABA). The effects of GABA are mediated by GABAA and/or GABAB receptors. While GABAB receptors in the central nervous system have now been extensively characterized, little is known of the function and pharmacology of GABAB receptors on endo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8381070
更新日期:1993-02-01 00:00:00
abstract::During early pseudopregnancy, DNA synthesis and mitosis in the uterine endometrial stroma precede the development of uterine sensitivity to deciduogenic stimuli. Progesterone redirects the effects of estradiol on endometrial DNA synthesis from the luminal epithelium to the stroma. To determine the time and hormonal co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1302
更新日期:1984-10-01 00:00:00
abstract::Benzodiazepines (BZs) have been shown to modulate voltage-sensitive Ca2+ channels in a number of neuronal and nonneuronal cell types and to competitively antagonize TRH binding to receptors on cells of the nervous system and anterior pituitary gland. Because interaction of TRH with its receptor is known to cause enhan...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-541
更新日期:1988-07-01 00:00:00
abstract::GnRH peptides in the hypothalami of marsupials (tammar wallaby, short-nosed bandicoot, and eastern quoll) and a monotreme (echidna) were investigated by reverse phase HPLC and RIA with region-specific antisera. In the wallaby hypothalamic extract, a single form of GnRH was present, which eluted in the same position as...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2244
更新日期:1989-11-01 00:00:00