Abstract:
:Although viral infection is thought to be associated with subacute thyroiditis and probably with autoimmune thyroid disease, possible changes in thyroid function during the prodromal period of infection or subclinical infection remain largely unknown. Recently, it was shown that pathogen-associated molecular patterns stimulate Toll-like receptors (TLR) and activate innate immune responses by producing type I interferons (IFN). Using a human thyroid follicle culture system, in which de novo synthesized thyroid hormones are released into the culture medium under physiological concentrations of human TSH, we studied the effects of polyinosinic-polycytidylic acid [Poly(I:C)], a chemical analog of viral double-stranded RNA (dsRNA), on TSH-induced thyroid function. Thyrocytes expressed ligands for dsRNA (TLR 3, CD14, and retinoic-acid-inducible protein-1) comparable with the TSH receptor. DNA microarray and real-time PCR analyses revealed that dsRNA increased the expression of mRNA for TLR3, IFN-beta, IFN-regulating factors, proinflammatory cytokines, and class I major histocompatibility complex (MHC), whereas genes associated with thyroid hormonogenesis (sodium/iodide symporter, peroxidase, deiodinases) were suppressed. In accordance to these data, Poly(I:C) suppressed TSH-induced 125I uptake and hormone synthesis dose dependently, accompanied by a decrease in the ratio of 125I-T3/125I-T4 released into the culture medium, whereas peptidoglycan, lipopolysaccharides, or unmethylated CpG DNA, ligands for TLR2, TLR4, and TLR9, respectively, had no significant effect. These inhibitory effects of Poly(I:C) were not blocked by a neutralizing antibody against TLR3 and an anti-IFN alpha/beta receptor antibody. These in vitro findings suggest that when thyrocytes are infected with certain viruses, dsRNA formed intracellularly in thyrocytes may be a cause for thyroid dysfunction, leading to development of autoimmune thyroiditis.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Yamazaki K,Suzuki K,Yamada E,Yamada T,Takeshita F,Matsumoto M,Mitsuhashi T,Obara T,Takano K,Sato Kdoi
10.1210/en.2006-1638subject
Has Abstractpub_date
2007-07-01 00:00:00pages
3226-35issue
7eissn
0013-7227issn
1945-7170pii
en.2006-1638journal_volume
148pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The calcium-dependent class of cell adhesion molecules known as cadherins mediate homotypic cell interactions in most epithelia. We have now investigated the expression and distribution of cadherins and cadherin-associated molecules in the developing and maturing rat testis. E-Cadherin was not detected in the seminife...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7507830
更新日期:1994-02-01 00:00:00
abstract::Cathepsin-D has been previously reported to cleave intact PTH into PTH-(1-34) and -(35-84) in membranous fractions of rat and bovine kidney. Whether PTH degradation occurs by intact kidney cells, however, has not been examined in detail. We have, therefore, examined this possibility using an opossum kidney (OK) cell l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2812
更新日期:1988-12-01 00:00:00
abstract::We recently found that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2))-regulated filamentous actin (F-actin) polymerization was diminished in hyperinsulinemic cell culture models of insulin resistance. Here we delineated whether increased glucose flux through the hexosamine biosynthesis pathway (HBP) ca...
journal_title:Endocrinology
pub_type: 杂志文章,收录出版
doi:10.1210/en.2008-1102
更新日期:2009-04-01 00:00:00
abstract::Although many studies have investigated the complex interrelationships between thyroid hormone levels and insulin-glucose secretion and action, there is at present a lack of information concerning the effects of various thyroid hormone levels on insulin receptors. In the present study, rat liver membranes were prepare...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-1922
更新日期:1982-06-01 00:00:00
abstract::Peptide-YY (PYY) is a novel enteric peptide that is structurally related to pancreatic polypeptide and neuropeptide-Y. The objectives of the present experiments were to characterize the following aspects of PYY metabolism: the distribution of PYY in the canine gastrointestinal tract, the release of PYY in response to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1438
更新日期:1989-03-01 00:00:00
abstract::The secretion of neurohypophyseal hormone and ACTH in the rat has been shown to exhibit circadian rhythms, with high values during the day and low values throughout the night. The neurohypophyseal hormone daily rhythm is altered by exposure to constant light and by pinealectomy. It was, thus, proposed that the observe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.8440190
更新日期:1993-03-01 00:00:00
abstract::Three distinct receptor types for natriuretic peptides (NP) have been identified in human tissue. "A" and "B" receptors initiate biological actions, whereas the "C" receptor has a clearance function. It has been proposed that the natural ligand for the B receptor is c-type natriuretic peptide (CNP), rather than atrial...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1309350
更新日期:1992-01-01 00:00:00
abstract::Obesity induced by high-fat (HF) feeding is associated with low-grade inflammation in peripheral tissues that predisposes to insulin resistance. Recent evidence suggests the occurrence of a similar process in the hypothalamus, which favors weight gain through impairment of leptin and insulin signaling. In addition to ...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2010-0336
更新日期:2010-09-01 00:00:00
abstract::Somatostatin (SRIF) inhibits vasoactive intestinal peptide (VIP)-stimulated cAMP accumulation in the GH4C1 strain of rat pituitary tumor cells, and this effect is responsible for SRIF inhibition of VIP-stimulated hormone release. In this study we examined the interaction between the SRIF receptor and adenylate cyclase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1784
更新日期:1984-05-01 00:00:00
abstract::Glucagon-like peptide-2 (GLP-2) is a nutrient-dependent proglucagon-derived hormone that stimulates intestinal growth through poorly understood paracrine and/or neural pathways. The relationship between GLP-2 action and a vagal pathway is unclear. Our aims were to determine whether 1) the GLP-2 receptor (GLP-2R) is ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1232
更新日期:2007-05-01 00:00:00
abstract::The present study was undertaken to determine whether T3 could modify anterior pituitary Ca++ metabolism under basal conditions and in the presence of TRH. Paired hemipituitaries from hypothyroid rats were preincubated with T3 (10(-7) M) for 2 h, allowed to accumulate 45Ca++ for the third hour, and repeatedly washed i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-5-1690
更新日期:1981-05-01 00:00:00
abstract::Multiple interferon (IFN)-tau genes exist in cattle, but it has remained unclear how many are expressed, the extent of their variation, and whether different genes exhibit similar patterns of expression and code for proteins with similar biological activities. A total of 118 complementary DNA (cDNA) were bi-directiona...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8249
更新日期:2001-07-01 00:00:00
abstract::Testes of 29-35-day-old rats were separated into seminiferous tubules and interstitial tissue by collagenase treatment and examined by incubation studies with radioactive substrates. The activity of testosterone 5alpha-reductase/g protein in the interstitial tissue was 37 times greater than that in the tubules. The si...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-3-918
更新日期:1978-03-01 00:00:00
abstract::Lipoprotein lipase (LPL) is a key regulator of triglyceride clearance. Its coordinated regulation during feeding and fasting is critical for maintaining lipid homeostasis and energy supply. Angiopoietin-like (Angptl)3 and Angptl4 are secreted proteins that have been demonstrated to regulate triglyceride metabolism by ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0476
更新日期:2005-11-01 00:00:00
abstract::Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3), the predominant IGF carrier protein in circulation, is posttranslationally modified in vivo by IGFBP-3 protease(s) into a number of fragments. Based on the ascertained and predicted recognition sites for known IGFBP-3 proteases, FLAG-epitope tagged intact I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7781
更新日期:2000-11-01 00:00:00
abstract::The present study was designed to investigate the effect of PTH on calcium (Ca2+) transport through the luminal membrane of proximal and distal rabbit tubule segments. Proximal tubule and distal tubule segment suspensions were incubated with the hormone, and the luminal membranes were subsequently purified. Incubation...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.1.7506210
更新日期:1994-01-01 00:00:00
abstract::5-Hydroxytryptamine (5-HT) is synthesized and secreted by thyroid parafollicular (PF) cells. As all PF granules contain 5-HT, it is released whenever PF cells secrete. Because 5-HT stimulates follicular (F) cells and can modulate their response to TSH, 5-HT has been proposed to be a paracrine PF to F cell transmitter....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.10.8828510
更新日期:1996-10-01 00:00:00
abstract::Developmental effects of genistein (Gen) on the mammary gland were investigated using outbred female CD-1 mice treated neonatally on d 1-5 by sc injections at doses of 0.5, 5, or 50 mg/kg.d. Examination of mammary gland whole mounts (no. 4) before puberty (4 wk) revealed no morphological differences in development aft...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0389
更新日期:2006-10-01 00:00:00
abstract::Owing to the prevalence of depression during childbearing, mothers can be prescribed multiple antidepressants; however, little is known about the risk and consequences to the offspring or subsequent generations. Fluoxetine (FLX) is usually the first-line of pharmacological treatment for affective disorders in pregnant...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00281
更新日期:2019-09-01 00:00:00
abstract::Thyroid papillary carcinoma is the most common type of endocrine cancer. It is frequently associated with genetic alterations leading to activation of the MAPK signaling pathway. The two most frequently affected genes, BRAF and RET, are activated by either point mutation or as a result of chromosomal rearrangement. Th...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0921
更新日期:2007-03-01 00:00:00
abstract::In amphibians, the secretion of alpha-MSH by melanotrope cells is stimulated by TRH and inhibited by NPY. We have previously shown that NPY abrogates the stimulatory effect of TRH on alpha-MSH secretion. The aim of the present study was to characterize the receptor subtypes mediating the action of NPY and to investiga...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.5.8761
更新日期:2002-05-01 00:00:00
abstract::Osteoblasts are known to produce osteoclast-stimulating activity (OSA). The aim of the current study was to relate the expression of OSA to the osteoblastic phenotype and examine its regulation by calciotropic hormones. The study was performed with the normal osteoblastic cell clone CRP 10/30 and the preosteoblastic c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.3.1505460
更新日期:1992-09-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::The contributions of estradiol and testosterone to atherosclerotic lesion progression are not entirely understood. Cross-sex hormone therapy (XHT) for transgender individuals dramatically alters estrogen and testosterone levels and consequently could have widespread consequences for cardiovascular health. Yet, no prec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00884
更新日期:2018-02-01 00:00:00
abstract::Photoaffinity labeling with underivatized T3 was used to identify T3-binding proteins in the membrane of rat erythrocytes. UV irradiation of ghosts and peripheral protein-depleted membranes in the presence of [125I]T3 resulted in the covalent attachment of 125I to membrane proteins (analyzed by polyacrylamide gel elec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8504750
更新日期:1993-06-01 00:00:00
abstract::Recent work has led to the hypothesis that kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the arcuate nucleus play a key role in GnRH pulse generation, with kisspeptin driving GnRH release and neurokinin B (NKB) and dynorphin acting as start and stop signals, respectively. In this study, we tested this hypothesis...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1331
更新日期:2013-11-01 00:00:00
abstract::Abnormally high GH/IGF-I levels, most often caused by adenomas arising from pituitary somatotrophs, generate deleterious effects. We recently described a targeted secretion inhibitor (SXN101742) comprising a GHRH domain and the endopeptidase domain of botulinum toxin serotype D (GHRH-light chain endopeptidase type D d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1427
更新日期:2013-11-01 00:00:00
abstract::Retinoic acid (RA) slows growth and induces differentiation of tumor cells through activation of RA receptors (RARs). However, melanoma cell lines display highly variable responsiveness to RA, which is a poorly understood phenomenon. By using Northern and Western blot analyses, we show that RA-resistant A375 and RA-re...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.6.8201
更新日期:2001-06-01 00:00:00
abstract::Posttranslational modifications of prolactin (PRL), including phosphorylation, vary with physiologic state and alter biologic activity. In light of the growing evidence for a role for PRL in proliferation in mammary cancer, we examined the ability of a mimic of phosphorylated human PRL, S179D-PRL, to initiate signals ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0826
更新日期:2003-12-01 00:00:00
abstract::Anterior pituitary cells from adult male rats were cultured on a natural extracellular matrix (ECM). From the 5th day, spindle-shaped cells, fusing to form elongated fibers, were observed among the epithelial cells. These fibers later increased in size and number and were identified as striated muscle fibers, based on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-3002
更新日期:1988-06-01 00:00:00