Evaluation of the thrombolytic effect of tissue-type plasminogen activator with ultrasonic irradiation: in vitro experiment involving assay of the fibrin degradation products from the clot.

abstract：:We have previously reported that INHAT (inhibitor of acetyltransferases) complex subunits, TAF (template activating factor)-Ialpha, TAF-Ibeta and pp32 can inhibit histone acetylation and HAT (histone acetyltransferase)-dependent transcription by binding to histones. Evidences are accumulating that INHAT complex subuni...

journal_title：Biological & pharmaceutical bulletin

authors： Lee IS,Oh SM,Kim SM,Lee DS,Seo SB

更新日期：2006-12-01 00:00:00

abstract：:Splenic marginal zone macrophages expressing macrophage receptor with collagenous structure (MARCO) contribute to the clearance of blood-borne pathogens. We determined a splenic adherent cell fraction abundantly containing cells expressing a higher level of MARCO by flow cytometry, and examined the effects of daily ad...

journal_title：Biological & pharmaceutical bulletin

authors： Shirato K,Sato S,Sato M,Hashizume Y,Tachiyashiki K,Imaizumi K

更新日期：2013-01-01 00:00:00

abstract：:Hypoxia-inducible factor-1 (HIF-1) is a central mediator of cellular responses to low oxygen and has recently become an important therapeutic target for solid tumor therapy. To identify small molecule inhibitors of the HIF-1 transcriptional activation, we have established a high through-put assay system using a stable...

journal_title：Biological & pharmaceutical bulletin

authors： Yamazaki Y,Hasebe Y,Egawa K,Nose K,Kunimoto S,Ikeda D

更新日期：2006-10-01 00:00:00

abstract：:An accumulating body of evidence indicates that inflammation plays a crucial role in the pathophysiology of myocardial infarction (MI). Nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome is an intracellular multiprotein complex that regulates caspase-1 a...

journal_title：Biological & pharmaceutical bulletin

authors： Takahashi M

更新日期：2019-01-01 00:00:00

abstract：:For the accurate identification of medicinal licorice species, nucleotide sequences of four types of DNA regions were researched for 205 specimens, including three species used as licorice: Glycyrrhiza uralensis, Glycyrrhiza glabra, and Glycyrrhiza inflata. The four DNA regions were the internal transcribed spacer (IT...

journal_title：Biological & pharmaceutical bulletin

authors： Kondo K,Shiba M,Yamaji H,Morota T,Zhengmin C,Huixia P,Shoyama Y

更新日期：2007-08-01 00:00:00

abstract：:To develop potent paclitaxel (PTX) formulations for cancer chemotherapy, we formulated PTX into polymeric nanoparticles composed of polyethylene glycol (PEG) and polylactic acid (PLA) block copolymer (PN-PTX). First, the physicochemical properties of PN-PTX prepared were assessed; the mean particle size was around 80 ...

journal_title：Biological & pharmaceutical bulletin

authors： Araki T,Kono Y,Ogawara K,Watanabe T,Ono T,Kimura T,Higaki K

更新日期：2012-01-01 00:00:00

abstract：:The genome sequencing project revealed presence of two active chalcone synthase (CHS) homologues (At1g02050 and At4g34850) in the model plant Arabidopsis thaliana. We report herein the two genes encode closely related novel plant-specific type III polyketide synthases (PKSs) that produces long-chain alkyl alpha-pyrone...

journal_title：Biological & pharmaceutical bulletin

authors： Mizuuchi Y,Shimokawa Y,Wanibuchi K,Noguchi H,Abe I

更新日期：2008-12-01 00:00:00

abstract：:It has been well known that Vibrio cholerae inhabit in environmental water. As many patients infected with cholera toxin-producing V. cholerae O1 (toxigenic V. cholerae O1) emerge in Kolkata, India, it has been thought that toxigenic V. cholerae O1 is easily detected in environmental water in Kolkata. However, we coul...

journal_title：Biological & pharmaceutical bulletin

authors： Paul SS,Takahashi E,Chowdhury G,Miyoshi SI,Mizuno T,Mukhopadhyay AK,Dutta S,Okamoto K

更新日期：2020-08-01 00:00:00

abstract：:Selective inhibitors of phosphodiesterases (PDEs) have been suggested to have anti-inflammatory effects on bronchial asthma through the inhibition of chemotaxis, adhesion, degranulation, the respiratory burst, and survival prolongation of eosinophils. However, the mechanisms by which these agents inhibit eosinophil su...

journal_title：Biological & pharmaceutical bulletin

authors： Wang W,Masu K,Tamura G,Suzuki K,Ohwada K,Okuyama K,Shirato K,Takayanagi M,Ohno I

更新日期：2005-03-01 00:00:00

abstract：:Monoclonal antibodies are widely used for the treatment of various diseases, and because therapeutic monoclonal antibodies are stored in an aqueous solution or in a lyophilized state, the preparation of a stabilizing formulation that prevents their deterioration (degradation and aggregation) is crucial. Given the stru...

journal_title：Biological & pharmaceutical bulletin

authors： Ishikawa T,Ito T,Endo R,Nakagawa K,Sawa E,Wakamatsu K

更新日期：2010-01-01 00:00:00

abstract：:B-type natriuretic peptide (BNP) is one peptide hormone released in response to myocyte stretch, whose functions play significant roles in health and disease. Its physiologic effects result in improved loading conditions and have led to the development of recombinant BNP as a therapeutic agent for heart failure. Previ...

journal_title：Biological & pharmaceutical bulletin

authors： Sun Y,Zhang Y,Yan M,Wu Y,Zheng X

更新日期：2009-09-01 00:00:00

abstract：:D-Aspartate (D-Asp), a free D-amino acid found in mammals, plays crucial roles in the neuroendocrine, endocrine, and central nervous systems. Recent studies have implicated D-Asp in the pathophysiology of infertility and N-methyl-D-Asp receptor-related diseases. D-Asp oxidase (DDO), a degradative enzyme that is stereo...

journal_title：Biological & pharmaceutical bulletin

authors： Katane M,Kawata T,Nakayama K,Saitoh Y,Kaneko Y,Matsuda S,Saitoh Y,Miyamoto T,Sekine M,Homma H

更新日期：2015-01-01 00:00:00

abstract：:We investigated the genotypic status of thiopurine methyltransferase (TPMT) polymorphism to evaluate the possible risk of the toxicity of azathioprine (AZA) in 68 patients with systemic lupus erythematosus (SLE). The allele frequency of TPMT mutation in the SLE group (2.9%) was higher than that in 174 Japanese healthy...

journal_title：Biological & pharmaceutical bulletin

authors： Okada Y,Nakamura K,Kodama T,Ueki K,Tsukada Y,Maezawa A,Tsukamoto N,Nojima Y,Ishizaki T,Horiuchi R,Yamamoto K

更新日期：2005-11-01 00:00:00

abstract：:Previous reports have indicated that isosteviol sodium (STVNa) has neuroprotective effects against acute focal cerebral ischemia in rats; however, the exact underlying mechanisms and ideal treatment paradigm are not known. To find a reasonable method for STVNa administration and to determine its possible therapeutic m...

journal_title：Biological & pharmaceutical bulletin

authors： Zhang H,Sun X,Xie Y,Tian F,Hu H,Tan W

更新日期：2018-01-01 00:00:00

abstract：:The object of this study was to investigate the binding affinity of a newly synthesized 5-HT2 antagonist, (N-[2-[4-(5H-dibenzo[a,d]cyclohepten-5-ylidene)-piperidino]ethyl]-1-formyl-4-piperidinecarboxamide monohydrochloride monohydrate) (AT-1015), in the rabbit platelet membrane using [3H]-ketanserin by radioligand bin...

journal_title：Biological & pharmaceutical bulletin

authors： Rashid M,Watanabe M,Nakazawa M,Nagatomo T

更新日期：2001-10-01 00:00:00

abstract：:The sialic acid binding lectin from bullfrog Rana catesbeiana oocyte (cSBL) is known to have anti-tumor activity. In order to investigate the relationship between the net charge of cSBL and its anti-tumor effect, cSBL was modified with a water-soluble carbodiimide (EDC) in the presence of three kinds of nucleophiles, ...

journal_title：Biological & pharmaceutical bulletin

authors： Iwama M,Ogawa Y,Sasaki N,Nitta K,Takayanagi Y,Ohgi K,Tsuji T,Irie M

更新日期：2001-09-01 00:00:00

abstract：:Costunolide, isolated from the stem bark of Magnolia sieboldii, is a sesquiterpene lactone that exhibits various biological and immunological actions. We investigated the induction mechanism of apoptosis by costunolide in a human B cell leukemia NALM-6 cell culture system. Costunolide (10 microM)-induced apoptosis tim...

journal_title：Biological & pharmaceutical bulletin

authors： Kanno S,Kitajima Y,Kakuta M,Osanai Y,Kurauchi K,Ujibe M,Ishikawa M

更新日期：2008-05-01 00:00:00

abstract：:Among the calcium-binding proteins from chicken gizzard smooth muscle, smooth muscle calcium-binding protein 11 (SMCaBP 11), which is an EF-hand type calcium-binding protein, can prevent Ca2+ dependent histamine release from permeabilized rat peritoneal mast cells. This activity appears to be specific since other calc...

journal_title：Biological & pharmaceutical bulletin

authors： Fujii T,Kuzumaki N,Ogoma Y,Kondo Y

更新日期：1994-05-01 00:00:00

abstract：:This study was conducted to determine the beneficial effects of treating digestive disorders of (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol diacetate (TDEYA) detected in the plasma in hydrolyzed form: (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol (TDEY), following the oral administration of a decoction of Atractylodes r...

journal_title：Biological & pharmaceutical bulletin

authors： Sakurai T,Sugawara H,Saito K,Kano Y

更新日期：1994-10-01 00:00:00

abstract：:Ibudilast (3-isobutyryl-2-isopropylpyrazolo[1,5-alpha]pyridine) is clinically used as a cerebral vasodilator in Japan. However, the effects of ibudilast on retinal blood vessels have not been fully examined. The aim of this study, therefore, was to examine the effects of ibudilast on retinal blood vessels in rats in v...

journal_title：Biological & pharmaceutical bulletin

authors： Noguchi M,Mori A,Sakamoto K,Nakahara T,Ishii K

更新日期：2009-11-01 00:00:00

abstract：:Three sialoglycoprotein fractions having reactivities against influenza A and B viruses (IV-A and IV-B) and influenza virus-hemagglutinin (IV-HA), were obtained by gel filtration using a Sephacryl S-300 column and an Asahipak GS-710 HPLC column from water-soluble fraction prepared from human meconium (ME-WSF). Molecul...

journal_title：Biological & pharmaceutical bulletin

authors： Ohyama K,Shinozuka T,Yanagida J,Yamakawa T

更新日期：1997-11-01 00:00:00

abstract：:High-resolution deep tissue imaging is possible with two-photon excitation microscopy. With the combined application of two-photon imaging and perfusion with a polar fluorescent tracer, we have established a method to detect exocytic events inside secretory tissues. This method displays the spatiotemporal distribution...

journal_title：Biological & pharmaceutical bulletin

authors： Takahashi N

更新日期：2015-01-01 00:00:00

abstract：:Glomerulonephritis (GN) is a set of pathological conditions that result in the destruction of glomeruli and loss of renal function, commonly leading to the development of end-stage renal disease. Current pharmacotherapy is limited to immunosuppressive therapy. In the present study, we found a novel antinephritic effec...

journal_title：Biological & pharmaceutical bulletin

authors： Liu N,Shi J,Xiao Y,Yasue M,Takei Y,Sanefuji H,Tsujimoto G,Hirasawa A

更新日期：2015-01-01 00:00:00

abstract：:Nafamostat mesilate, an ester drug with extensive hydrolysis in vivo, exhibits species difference in the relative contribution for its hydrolysis in blood and tissues. For the rat, the main hydrolysis site may be blood and human may be tissue (mainly by liver). The paper gave in vivo evidence that human tissue may giv...

journal_title：Biological & pharmaceutical bulletin

authors： Cao YG,Chen YC,Hao K,Zhang M,Liu XQ

更新日期：2008-11-01 00:00:00

abstract：:PEGylation is an established method for improving the pharmacokinetic properties and pharmacodynamic effects of therapeutic proteins. Amino conjugation by N-hydroxysuccinimide activated polyethylene glycol (PEG-NHS) represents the most common modification of the target proteins. In this study, we compared the reaction...

journal_title：Biological & pharmaceutical bulletin

authors： Nojima Y,Iguchi K,Suzuki Y,Sato A

更新日期：2009-03-01 00:00:00

abstract：:Uridine diphosphate (UDP)-glucuronosyltransferase 1A1 (UGT1A1) plays important roles in the glucuronidation of various drugs and endogenous substances. Minipigs have been used as experimental animals in pharmacological and toxicological studies, because many of their physiological characteristics are similar to those ...

journal_title：Biological & pharmaceutical bulletin

authors： Miyake Y,Mayumi K,Jinno H,Tanaka-Kagawa T,Narimatsu S,Hanioka N

更新日期：2013-01-01 00:00:00

abstract：:Nitrogen oxide, which is produced by activated macrophages, has been demonstrated to possess anti-tumor activity. We report herein the synergistic effect of sodium nitrite (NO2-) and/or sodium nitroprusside (SNP) on lysophospholipid (LysoPL)-mediated cytolysis. The incubation of 51Cr-labeled mouse melanoma (B16) cells...

journal_title：Biological & pharmaceutical bulletin

authors： Kanematsu M,Takagi K,Suketa Y

更新日期：1994-01-01 00:00:00

abstract：:Aberrant wound healing, either causing scarring or chronic wounds, is a significant cause of morbidity. There is therefore, considerable interest in agents which can modulate certain aspects of the wound healing process. Fucoidans, sulphated polyfucose polysaccharides which may be extracted from Fucus spp., have been ...

journal_title：Biological & pharmaceutical bulletin

authors： O'Leary R,Rerek M,Wood EJ

更新日期：2004-02-01 00:00:00

abstract：:The present study investigated whether or not Sho-saiko-to (crude powder extract, TJ-9) can suppress nitric oxide (NO) generation by endotoxin-activated J774A.1 cells in order to study the preventive mechanism of Sho-saiko-to against endotoxemia. In this experiment, we estimated the NO2- in the murine macrophage cell ...

journal_title：Biological & pharmaceutical bulletin

authors： Sakaguchi S,Furusawa S,Yokota K,Sasaki K,Takayanagi Y

更新日期：1995-04-01 00:00:00

abstract：:We investigated the gene expression of GAPDH, cytochrome P450 isoforms (CYP1A1, CYP1A2, CYP1B1, CYP2A6, CYP2E1, CYP3A4, CYP3A5, and CYP3A7), UGT-dependent glucuronosyl transferase isoforms (UGT1A6 and UGT1A9), and ABC transporter (ABCB1) after exposure to 0.1, 0.5, and 2.5% dimethyl sulfoxide (DMSO). GAPDH mRNA levels...

journal_title：Biological & pharmaceutical bulletin

authors： Nishimura M,Ueda N,Naito S

更新日期：2003-07-01 00:00:00