Abstract:
:Our goal has been to develop a safe and effective system that would allow us to explore the functions of the human immunodeficiency virus (HIV) envelope. We have generated a human lymphoid cell line (TF228.1.16) that stably expresses functional HIV envelope proteins on its cell surface, and therefore closely mimics the viral envelope and virus-infected cells. The TF228.1.16 line forms syncytia with human cells of the CD4+ phenotype and provides a facile virus-free cell-based assay for examining the mechanism of syncytia formation and for evaluating novel agents that may disrupt this process. The TF228.1.16 cells also provide an opportunity to present the HIV envelope proteins to the immune system in cellular form. In vitro immunization of human peripheral blood mononuclear cells (PBMC) and in vivo immunization of rhesus monkeys with this reagent results in the production of antibodies with neutralizing (anti-syncytia) activities. When the HIV envelope is expressed against the background of human lymphoid cells, it may exhibit immune protection with unique properties that have not yet been explored. Our results indicate that a virus-free cell system can play an important role in exploring the biology and function of HIV-envelope proteins without the interference of other viral components present in infected cells. This paper discusses these results, and examines the potential use of TF228.1.16 as a vaccine.
journal_name
AIDS Res Hum Retrovirusesjournal_title
AIDS research and human retrovirusesauthors
Jonak ZL,Clark RK,Matour D,Trulli S,Craig R,Henri E,Lee EV,Greig R,Debouck Cdoi
10.1089/aid.1993.9.23subject
Has Abstractpub_date
1993-01-01 00:00:00pages
23-32issue
1eissn
0889-2229issn
1931-8405journal_volume
9pub_type
杂志文章abstract::Epidemic modeling suggests that a major scale-up in HIV treatment could have a dramatic impact on HIV incidence. This has led both researchers and policymakers to set a goal of an "AIDS-Free Generation." One of the greatest obstacles to achieving this objective is the number of people with undiagnosed HIV infection. D...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章,评审
doi:10.1089/AID.2013.0274
更新日期:2014-03-01 00:00:00
abstract::Vaginal SHIVSF162P3 acquisition in pigtail macaques (Macaca nemestrina) is dependent on time point during the menstrual cycle. Susceptibility is higher around menstruation and lower at ovulation in mid cycle. This complicates the design of repeat low-dose (RLD) SHIV exposure studies because virus challenges given duri...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2014.0373
更新日期:2015-11-01 00:00:00
abstract::Accurate methods for cross-sectional incidence estimation are needed for HIV prevention research. The Limiting Antigen Avidity (LAg-Avidity) assay has been marketed by two vendors, Maxim Biomedical and Sedia BioSciences Corporation. Performance differences between the two versions of the assay are unknown. We tested a...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2016.0245
更新日期:2017-06-01 00:00:00
abstract::We studied vertical transmission of feline immunodeficiency virus (FIV) to determine whether it might provide a model with which to study intervention strategies for mother-to-offspring transmission of human immunodeficiency virus (HIV). We found that pregnant cats acutely infected with FIV (FIV-CSU-2771) transmitted ...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1995.11.171
更新日期:1995-01-01 00:00:00
abstract::A total of 110 HIV-1-positive samples obtained in 1997 (n = 44) and 2000 (n = 66) were genetically characterized in the V3-V5 envelope region and the p24 gag region. The majority of the strains were CRF02-AG (54.3%) or CRF06-cpx (18.1%) in env and gag. More than 9% of the samples were recombinants between CRF02 and CR...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922202760072357
更新日期:2002-07-01 00:00:00
abstract::The HIV-1 Nef protein is known to be secreted, and our group has shown that Nef is secreted from nef-transfected and HIV-1-infected cells in small exosome-like vesicles (d. 40-100 nm). The role of secreted Nef remains to be fully characterized. Thus, it is important to characterize the nature of and the mechanisms reg...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2009.0068
更新日期:2010-02-01 00:00:00
abstract::To help in the vaccine development, WHO-UNAIDS launched a program for the isolation and characterization of subtype C viruses, the most prevalent HIV-1 subtype in the world. Isolates were obtained from Brazil, China, India, Israel, and South Africa, countries in which these strains are circulating. In this study we ge...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/08892220360473961
更新日期:2003-01-01 00:00:00
abstract::The transmembrane envelope (TM) protein gp41 of HIV-1 is an attractive target when designing a vaccine to induce neutralizing antibodies. A few broadly neutralizing antibodies (2F5, 4E10, and 10E8) that target conserved epitopes in the membrane proximal external region (MPER) of gp41 have been isolated from infected i...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2013.0191
更新日期:2014-05-01 00:00:00
abstract::Subunit vaccines based on recombinant proteins have proved useful for inducing antibody responses and they are safe for widespread use because they do not contain any live components. Unfortunately, they do not typically induce the types of cell-mediated immune responses required to control viral pathogens; specifical...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1994.10.853
更新日期:1994-07-01 00:00:00
abstract::We have previously shown that the cell line 6D5(451) chronically infected with the HIV-1 isolate HTLV-III(451), secretes the HIV-1 envelope glycoproteins gp120 and gp160 in the extracellular medium. The HTLV-III(451) gp120 and gp160 were purified by sequential affinity chromatographic steps using a monoclonal antibody...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1990.6.371
更新日期:1990-03-01 00:00:00
abstract::To understand the evolution of HIV-1, the genetic and biological characteristics of viruses that infect persons living in regions in which the virus has been evolving for several decades must be studied. Thus, we investigated teh genetic subtypes, coreceptor usage, and syncytium-inducing ability of viruses in 47 HIV-1...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922203771881284
更新日期:2003-12-01 00:00:00
abstract::To establish whether the low cysteine and glutathione levels in HIV-infected patients and SIV-infected rhesus macaques may be consequences of an abnormal cysteine catabolism, we analyzed sulfate and glutathione levels in macaques. Muscle tissue (m. vastus lateralis and m. gastrocnemius) of SIV-infected macaques (n = 2...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1996.12.1639
更新日期:1996-11-20 00:00:00
abstract::As antiretroviral therapy continues to scale-up in developing countries, there is concern that high levels of HIV drug resistance to antiretroviral drugs will occur. Here we describe rates of emergence of HIV-1 drug resistance and factors associated with their occurrence among adults who received antiretroviral therap...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2008.0273
更新日期:2009-05-01 00:00:00
abstract::Sexual transmission of human immunodeficiency virus provides an efficient mode for virus spread and poses unique challenges to vaccine developers. Host and viral factors that affect transmission have been studied by epidemiological approaches in the human population, and some of these factors have been modeled with ex...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The mechanisms responsible for the hematopoietic failure in human immunodeficiency virus type 1 (HIV-1)-infected patients are still unknown. Several findings indicate that the in vitro proliferative potential of precursor cells from AIDS patients is reduced. The changes seen in bone marrow (BM) morphology and the defe...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922200750005994
更新日期:2000-10-10 00:00:00
abstract::To assess the impact of chronic viral liver disease (CVLD) on hospital admissions and death in HIV-infected patients since the introduction of highly active antiretroviral therapy, all hospital charts, from January 1996 to December 2000, in a large HIV/AIDS reference center in Madrid were reviewed. Discharge diagnosis...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/08892220152644160
更新日期:2001-11-01 00:00:00
abstract::HIV-1 disease is associated with many infectious and neoplastic complications. This is particularly true of the genitourinary tract, where high rates of renal disease, neoplasms, voiding and erectile dysfunction, hematuria, opportunistic genitourinary infections, and epididymo-orchitis are found in HIV-1-infected indi...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::We report here the gene for DC-SIGN from Chinese rhesus macaques. DC-SIGN is a C-type lectin expressed by dendritic cells (DCs). It is involved in the interaction of DCs with T cells, and in transmission to T cells of HIV-1 and SIV. Alternative splicing in human DC-SIGN yields A and B isoforms of the protein. The over...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922202760265641
更新日期:2002-09-01 00:00:00
abstract::Here we report the construction, sequencing, and biological characterization of a molecular clone of HIV-1(92UG001), a virus representative of subtype D strains circulating in Uganda. The virus produced by the clone has an aggressive syncytium-inducing phenotype, which matches that of the parental virus. This phenotyp...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922202753394754
更新日期:2002-01-01 00:00:00
abstract::The concept that HIV causes AIDS only by directly killing CD4 cells has been questioned by a number of investigators. There has been experimental support for a number of indirect mechanisms such as apoptosis, anergy, superantigen-induced cell proliferation and depletion, defective signaling, molecular mimicry, and aut...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章,评审
doi:10.1089/aid.1993.9.287
更新日期:1993-03-01 00:00:00
abstract::This retrospective and longitudinal study evaluated the long-term hepatic tolerance of a nelfinavir (NFV)-antiretroviral combined regimen in 82 patients of the HCV-HIV Cohort of CISIH-Sud of Marseilles. Follow-up data (liver enzyme levels, CD4 cell count, HIV viral load, and metabolic parameters) of patients treated w...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2005.21.841
更新日期:2005-10-01 00:00:00
abstract::Cell activation is essential for HIV infection. CD4+ T lymphocyte activation allows virus replication and CD8+ T lymphocyte activation may contribute to pathogenesis. We combined hydroxyurea, a cytostatic drug that inhibits cell activation and proliferation, with two drugs that inhibit HIV (didanosine and indinavir), ...
journal_title:AIDS research and human retroviruses
pub_type: 临床试验,杂志文章
doi:10.1089/088922299310917
更新日期:1999-05-01 00:00:00
abstract::The Northeastern Brazilian region has experienced a constant increase in the number of newly reported AIDS cases over the last decade, but the genetic diversity of HIV-1 strains currently disseminated in this region remains poorly explored. HIV-1 pol sequences were obtained from 140 patients followed at outpatient cli...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2017.0045
更新日期:2017-07-01 00:00:00
abstract::Similarly to HIV-infected cells, recombinant HIV-1 glycoprotein 120 induces acid-labile interferon production in peripheral blood mononuclear cells from healthy donors. Acid lability of this interferon is due to the presence of both IFN-alpha and -gamma molecules. In fact, although not revealed by neutralization of an...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1993.9.957
更新日期:1993-10-01 00:00:00
abstract::The nucleotide sequence of the gp41 transmembrane protein coding region of human immunodeficiency virus type 1 (HIV-1) proviral DNA obtained from blood and cerebrospinal fluid (CSF) from 6 individuals was determined by direct sequencing of polymerase chain reaction (PCR)-amplified DNA. The direct sequencing approach w...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1992.8.53
更新日期:1992-01-01 00:00:00
abstract::This study explores whether previous failures on antiretroviral drug regimens preclude the possibility of immune restoration. This was assessed by evaluating T cell subset changes in individuals who received a salvage regimen of highly active antiretroviral therapy (HAART) after initially failing protease inhibitor mo...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1998.14.561
更新日期:1998-05-01 00:00:00
abstract::Assays to determine HIV incidence from cross-sectional surveys have exhibited a high rate of false-recent misclassification in Kenya and Uganda where HIV subtypes A and D predominate. Samples from individuals infected with HIV for at least 2 years with known infecting subtype (133 subtype A, 373 subtype D) were tested...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2012.0105
更新日期:2013-08-01 00:00:00
abstract::Activation of primary CD4+ T cells induces the CD155, but not the CD112 ligands for the natural killer (NK) cell activation receptor (aNKR) CD226 [DNAX accessory molecule-1 (DNAM-1)]. We hypothesize that HIV productively infects activated CD4+ T cells and makes itself vulnerable to NK cell-mediated lysis when CD155 on...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2015.0375
更新日期:2017-02-01 00:00:00
abstract::Lectins with specificity for terminal mannose residues and anti-mannan antibodies neutralize HIV-1 infection in vitro. This is assumed to be caused by binding of the agents to the viral glycoproteins. In this study we show that one such agent, the Galanthus nivalis lectin (GNA), also blocks infection at the target cel...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1995.11.87
更新日期:1995-01-01 00:00:00
abstract::Macrophages are a preferred target for sexually transmitted human immunodeficiency virus type 1 (HIV-1) isolates that use CCR5 as a coreceptor in combination with CD4. To assess whether the susceptibility of MDMs to infection by an R5 isolate was influenced by CD4 and/or CCR5 expression, levels of membrane CD4 or CCR5...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922299310494
更新日期:1999-07-20 00:00:00