Abstract:
:Microsatellites have become the most important class of markers for mapping procedures. Primarily based on restriction fragment length polymorphism (RFLP) markers, several molecular genetic maps of maize have been developed, mainly using temperate inbred maize lines. To characterize the level of polymorphism of microsatellite loci and construct a genetic map in tropical maize, two elite inbred lines, L-08-05F and L-14-4B, were crossed to produce 400 F(2) individuals that were used as a mapping population. A survey of 859 primer pair sequences of microsatellites was used. The polymorphism screens of each microsatellite and genotype assignment were performed using high-resolution agarose gels. About 54 % of the primer sets gave clearly scorable amplification products, 13 % did not amplify and 33 % could not be scored on agarose gels. A total of 213 polymorphic markers were identified and used to genotype the mapping population. Among the polymorphic markers, 40 showed loci deviating from expected Mendelian ratios and clusters of deviating markers were located in three chromosome regions. Non-Mendelian scoring was present in 19 markers. The final genetic map with 117 markers spanned 1634 cM in length with an average interval of 14 cM between adjacent markers.
journal_name
Hereditasjournal_title
Hereditasauthors
Sibov ST,de Souza CL Jr,Garcia AA,Garcia AF,Silva AR,Mangolin CA,Benchimol LL,de Souza APdoi
10.1111/j.1601-5223.2003.01666.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
96-106issue
2eissn
0018-0661issn
1601-5223pii
HRD1666journal_volume
139pub_type
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