Mouse model of microembolic stroke and reperfusion.


BACKGROUND AND PURPOSE:To test the role of fibrinolysis in stroke, we used a mouse model in which preformed 2.5- to 3-microm-diameter fibrin microemboli are injected into the cerebral circulation. The microemboli lodge in the downstream precapillary vasculature and are susceptible to fibrinolysis. METHODS:We injected various doses of microemboli into the internal carotid artery in mice and characterized their distribution, effects on cerebral blood flow, neurological deficit, infarct area, and spontaneous dissolution. By comparing wild-type and tissue plasminogen activator (tPA) knockout (tPA-/-) mice, we analyzed the role of endogenous tPA in acute thrombotic stroke. RESULTS:Microemboli cause dose-dependent brain injury. Although moderate doses of microemboli are followed by spontaneous reperfusion, they result in reproducible injury. Gene knockout of tPA markedly delays dissolution of cerebral emboli and restoration of blood flow and aggravates ischemic thrombotic infarction in the brain. CONCLUSIONS:We describe a microembolic model of stroke, in which degree of injury can be controlled by the dose of microemboli injected. Unlike vessel occlusion models, this model can be modulated to allow spontaneous fibrinolysis. Application to tPA-/- mice supports a key role of endogenous tPA in restoring cerebral blood flow and limiting infarct size after thrombosis.






Atochin DN,Murciano JC,Gürsoy-Ozdemir Y,Krasik T,Noda F,Ayata C,Dunn AK,Moskowitz MA,Huang PL,Muzykantov VR




Has Abstract


2004-09-01 00:00:00
















  • Reduced vitamin D in acute stroke.

    abstract:BACKGROUND AND PURPOSE:Stroke leads to a reduction in bone mineral density, altered calcium homeostasis, and an increase in hip fractures. Vitamin D deficiency is well documented in long-term stroke survivors and is associated with post-stroke hip fractures. Less is known regarding levels in acute stroke. METHODS:We c...


    pub_type: 杂志文章


    authors: Poole KE,Loveridge N,Barker PJ,Halsall DJ,Rose C,Reeve J,Warburton EA

    更新日期:2006-01-01 00:00:00

  • Possible effect of DRGs on the classification of stroke: implications for epidemiological surveillance.

    abstract:BACKGROUND AND PURPOSE:Accurate data on the distribution of stroke subtypes are essential for understanding the forces driving recent morbidity and mortality trends. The introduction of diagnosis-related groups (DRGs) in the 1980s may have affected the distribution of stroke subtypes as defined by International Classif...


    pub_type: 杂志文章


    authors: Derby CA,Lapane KL,Feldman HA,Carleton RA

    更新日期:2001-07-01 00:00:00

  • Report of the substudy assessing the impact of neurocognitive function on quality of life 5 years after cardiac surgery.

    abstract:BACKGROUND AND PURPOSE:The importance of perioperative cognitive decline has long been debated. We recently demonstrated a significant correlation between perioperative cognitive decline and long-term cognitive dysfunction. Despite this association, some still question the importance of these changes in cognitive funct...


    pub_type: 杂志文章


    authors: Newman MF,Grocott HP,Mathew JP,White WD,Landolfo K,Reves JG,Laskowitz DT,Mark DB,Blumenthal JA,Neurologic Outcome Research Group and the Cardiothoracic Anesthesia Research Endeavors (CARE) Investigators of the Duke Heart Center.

    更新日期:2001-12-01 00:00:00

  • Three-dimensional MRI analysis of individual volume of Lacunes in CADASIL.

    abstract:BACKGROUND AND PURPOSE:Three-dimensional MRI segmentation may be useful to better understand the physiopathology of lacunar infarctions. Using this technique, the distribution of lacunar infarctions volumes has been recently reported in patients with cerebral autosomal-dominant arteriopathy with subcortical infarcts an...


    pub_type: 杂志文章


    authors: Hervé D,Godin O,Dufouil C,Viswanathan A,Jouvent E,Pachaï C,Guichard JP,Bousser MG,Dichgans M,Chabriat H

    更新日期:2009-01-01 00:00:00

  • Thrombotic risk factors in primary antiphospholipid syndrome: a 5-year prospective study.

    abstract:BACKGROUND AND PURPOSE:Because thromboembolic events are frequently observed in primary antiphospholipid syndrome (PAPS), we assessed the risk factors for new thrombotic episodes. METHODS:Fifty-six PAPS patients (mean age, 37+/-10 years) were prospectively studied for 5 years. The preliminary Sapporo classification cr...


    pub_type: 杂志文章


    authors: Turiel M,Sarzi-Puttini P,Peretti R,Rossi E,Atzeni F,Parsons W,Doria A

    更新日期:2005-07-01 00:00:00

  • Glutamate-induced cerebral vasodilation is mediated by nitric oxide through N-methyl-D-aspartate receptors.

    abstract:BACKGROUND AND PURPOSE:It was found that glutamate, a major neurotransmitter, is vasoactive in the cerebral circulation. However, the mechanism is unclear. This study was designed to investigate the role of nitric oxide (NO) and N-methyl-D-aspartate (NMDA) receptors in cerebral arteriolar dilation to glutamate. METHOD...


    pub_type: 杂志文章


    authors: Meng W,Tobin JR,Busija DW

    更新日期:1995-05-01 00:00:00

  • Positive platelet scintigram of a vertebral aneurysm presenting thromboembolic transient ischemic attacks.

    abstract::We describe a patient with transient ischemic attacks secondary to a giant aneurysm who showed increased activity on platelet scintigrams at the origin of the left vertebral artery. This is assumed to be the first report of a presumed embolizing aneurysm with positive activity of labeled platelets. Platelet scintigrap...


    pub_type: 杂志文章


    authors: Maruyama M,Asai T,Kuriyama Y,Sawada T,Ogata J,Nishimura T,Omae T

    更新日期:1989-05-01 00:00:00

  • Ischemic Stroke and Bleeding: Clinical Benefit of Anticoagulation in Atrial Fibrillation After Intracerebral Hemorrhage.

    abstract::Background and Purpose- Patients with intracerebral hemorrhage (ICH) and atrial fibrillation (AF) are at risk for ischemic events. While risk calculators (CHA2DS2-VASc and HAS-BLED) have been validated to assess risk for ischemic stroke and major bleeding in AF patients, decisions about anticoagulation must consider t...


    pub_type: 杂志文章


    authors: Stanton RJ,Eckman MH,Woo D,Moomaw CJ,Haverbusch M,Flaherty ML,Kleindorfer DO

    更新日期:2020-03-01 00:00:00

  • Stent-assisted reconstructive endovascular repair of cranial fusiform atherosclerotic and dissecting aneurysms: long-term clinical and angiographic follow-up.

    abstract:BACKGROUND AND PURPOSE:The purpose of this study was to investigate the periprocedural morbidity, mortality, and long-term clinical and angiographic follow-up using stent-assisted coiling and stenting alone for treatment of cranial fusiform dissecting and atherosclerotic aneurysms. METHODS:The Institutional Review Boa...


    pub_type: 杂志文章


    authors: Wakhloo AK,Mandell J,Gounis MJ,Brooks C,Linfante I,Winer J,Weaver JP

    更新日期:2008-12-01 00:00:00

  • Intra-arterial thrombolytic therapy improves outcome in patients with acute vertebrobasilar occlusive disease.

    abstract::In this retrospective analysis we report our treatment experience in 65 consecutive patients with clinical signs of severe brainstem ischemia with angiographically demonstrated thrombotic vertebrobasilar artery occlusions who received either local intra-arterial thrombolytic therapy (urokinase or streptokinase) (43 pa...


    pub_type: 杂志文章


    authors: Hacke W,Zeumer H,Ferbert A,Brückmann H,del Zoppo GJ

    更新日期:1988-10-01 00:00:00

  • Parity and risk of subarachnoid hemorrhage in women: a nested case-control study based on national Swedish registries.

    abstract:BACKGROUND AND PURPOSE:Subarachnoid hemorrhage (SAH) is the only type of stroke with female predominance, suggesting that reproductive factors may play a role in the etiology. We conducted a population-based study to examine the influence of parity on the risk of SAH in women. METHODS:We linked data from 3 national Sw...


    pub_type: 杂志文章


    authors: Gaist D,Pedersen L,Cnattingius S,Sørensen HT

    更新日期:2004-01-01 00:00:00

  • Homocysteine and pulsatility index of cerebral arteries.

    abstract:BACKGROUND AND PURPOSE:A pulsatility index (PI) represents vascular resistance distal to an examined artery. The purpose of the present study was to evaluate an association between plasma total homocysteine (tHcy) and PIs of the cerebral arteries in patients with ischemic stroke. METHODS:Consecutive patients with isch...


    pub_type: 杂志文章


    authors: Lim MH,Cho YI,Jeong SK

    更新日期:2009-10-01 00:00:00

  • Circadian fluctuations in onset of subarachnoid hemorrhage. New data on aneurysmal and perimesencephalic hemorrhage and a systematic review.

    abstract:BACKGROUND AND PURPOSE:Several studies have found a morning peak in onset of stroke. A similar peak has been suggested for subarachnoid hemorrhage (SAH), which seems in contradiction with the physical activities associated with aneurysmal rupture. Most studies included only a few patients with SAH. We analyzed time of ...


    pub_type: 杂志文章,评审


    authors: Vermeer SE,Rinkel GJ,Algra A

    更新日期:1997-04-01 00:00:00

  • Middle cerebral artery occlusion in rats studied by magnetic resonance imaging.

    abstract::Ischemia due to middle cerebral artery occlusion was studied in 29 rats from 1 to 24 hours after occlusion using magnetic resonance imaging. Images were made before and after the injection of a superparamagnetic iron oxide compound, AMI-25. Subtraction images demonstrated the region of perfusion deficit as early as 1 ...


    pub_type: 杂志文章


    authors: Bradley RH,Kent TA,Eisenberg HM,Quast MJ,Ward GA,Campbell GA,Hillman G

    更新日期:1989-08-01 00:00:00

  • Photothrombotic middle cerebral artery occlusion and reperfusion laser system in spontaneously hypertensive rats.

    abstract:BACKGROUND AND PURPOSE:To establish a less invasive and reproducible focal ischemia model in the rat, we adopted a 2-laser system (ie, photothrombosis and YAG laser-induced reperfusion). METHODS:The distal middle cerebral artery (MCA) of spontaneously hypertensive rats was occluded by 568-nm krypton laser and intraven...


    pub_type: 杂志文章


    authors: Yao H,Sugimori H,Fukuda K,Takada J,Ooboshi H,Kitazono T,Ibayashi S,Iida M

    更新日期:2003-11-01 00:00:00

  • Association of parental stroke with brain injury and cognitive measures in offspring: the Framingham Heart Study.

    abstract:BACKGROUND AND PURPOSE:Parental stroke has been related to an increased risk of stroke in the offspring. This study examines whether parental stroke is also associated with increased vascular brain injury and poorer cognitive performance among offspring free of clinical stroke. METHODS:Multivariable regression analyse...


    pub_type: 杂志文章


    authors: Weinstein G,Beiser AS,Au R,Decarli C,Wolf PA,Seshadri S

    更新日期:2013-03-01 00:00:00

  • Endovascular clot retrieval therapy: implications for the organization of stroke systems of care in North America.

    abstract::Endovascular acute ischemic stroke therapy is now proven by randomized controlled trials to produce large, clinically meaningful benefits. In response, stroke systems of care must change to increase timely and equitable access to this therapy. In this review, we provide a North American perspective on implications for...


    pub_type: 杂志文章,评审


    authors: Smith EE,Schwamm LH

    更新日期:2015-06-01 00:00:00

  • Mechanisms of perioperative cerebral infarction.

    abstract::Perioperative cerebral infarction occurs in less than 1% of general surgical procedures; the mechanism is usually unknown. The clinical features of 12 consecutive perioperative strokes were retrospectively reviewed. Although intraoperative hypotension was frequent, onset of deficit occurred postoperatively in 83% and ...


    pub_type: 杂志文章


    authors: Hart R,Hindman B

    更新日期:1982-11-01 00:00:00

  • Depression and antidepressant use after stroke and transient ischemic attack.

    abstract:BACKGROUND AND PURPOSE:Patients with stroke and transient ischemic attack (TIA) often have comparable comorbidities, but it is unclear whether they have similar rates of depression or antidepressant use. METHODS:This study was a secondary analysis of a prospective cohort registry that enrolled subjects from 2006 to 20...


    pub_type: 临床试验,杂志文章,多中心研究


    authors: El Husseini N,Goldstein LB,Peterson ED,Zhao X,Pan W,Olson DM,Zimmer LO,Williams JW Jr,Bushnell C,Laskowitz DT

    更新日期:2012-06-01 00:00:00

  • Biophysical mechanisms of stroke.

    abstract:BACKGROUND:Stroke is the third leading cause of death and the leading cause of long-term disability in the United States. Although a host of genetic, biochemical, physiological, anatomic, and histological factors have been implicated, to varying degrees, in the pathogenesis of stroke, biophysical factors are believed t...


    pub_type: 杂志文章,评审


    authors: Hademenos GJ,Massoud TF

    更新日期:1997-10-01 00:00:00

  • Plaque ulceration and lumen thrombus are the main sources of cerebral microemboli in high-grade internal carotid artery stenosis.

    abstract:BACKGROUND AND PURPOSE:Previous work has shown that rates of cerebral microemboli downstream of high-grade internal carotid artery stenosis are higher in recently symptomatic compared with asymptomatic patients. In addition, microembolic rates decline after carotid endarterectomy. We conducted a prospective investigati...


    pub_type: 杂志文章


    authors: Sitzer M,Müller W,Siebler M,Hort W,Kniemeyer HW,Jäncke L,Steinmetz H

    更新日期:1995-07-01 00:00:00

  • Predictors of early arterial reocclusion after tissue plasminogen activator-induced recanalization in acute ischemic stroke.

    abstract:BACKGROUND AND PURPOSE:We aimed to determine clinical and hemodynamic predictors of early reocclusion (RO) in stroke patients treated with intravenous tissue plasminogen activator (tPA). METHODS:We studied 142 consecutive stroke patients with a documented middle cerebral artery (MCA) occlusion treated with intravenous...


    pub_type: 杂志文章


    authors: Rubiera M,Alvarez-Sabín J,Ribo M,Montaner J,Santamarina E,Arenillas JF,Huertas R,Delgado P,Purroy F,Molina CA

    更新日期:2005-07-01 00:00:00

  • Low-dose recombinant tissue-type plasminogen activator enhances clot resolution in brain hemorrhage: the intraventricular hemorrhage thrombolysis trial.

    abstract:BACKGROUND AND PURPOSE:Patients with intracerebral hemorrhage and intraventricular hemorrhage have a reported mortality of 50% to 80%. We evaluated a clot lytic treatment strategy for these patients in terms of mortality, ventricular infection, and bleeding safety events, and for its effect on the rate of intraventricu...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Naff N,Williams MA,Keyl PM,Tuhrim S,Bullock MR,Mayer SA,Coplin W,Narayan R,Haines S,Cruz-Flores S,Zuccarello M,Brock D,Awad I,Ziai WC,Marmarou A,Rhoney D,McBee N,Lane K,Hanley DF Jr

    更新日期:2011-11-01 00:00:00

  • Agitated confusional states in patients with right hemisphere infarctions.

    abstract::Patients with infarctions in the territory of the right middle cerebral artery (RMCA) sometimes present with an agitated confusional state. We reviewed clinical data on 46 patients with RMCA infarcts and compared neurologic findings in patients with and without agitated confusion. Neither of the two patients presentin...


    pub_type: 杂志文章


    authors: Schmidley JW,Messing RO

    更新日期:1984-09-01 00:00:00

  • Cerebrovascular reserve in patients with carotid occlusive disease assessed by stable xenon-enhanced ct cerebral blood flow and transcranial Doppler.

    abstract:BACKGROUND AND PURPOSE:Cerebrovascular reserve (CVR) by both transcranial Doppler ultrasonography (TCD) and quantitative cerebral blood flow (CBF) can identify subgroups of patients at increased risk for stroke. A direct comparison of CVR measurements obtained with both technologies in patients with cerebrovascular occ...


    pub_type: 临床试验,杂志文章


    authors: Pindzola RR,Balzer JR,Nemoto EM,Goldstein S,Yonas H

    更新日期:2001-08-01 00:00:00

  • VEGF receptor-2 variants are associated with susceptibility to stroke and recurrence.

    abstract:BACKGROUND AND PURPOSE:Dysregulation of vessel wall formation, growth, and maintenance may confer susceptibility of stroke. METHODS:We tested the hypothesis that variants in 2 genes encoding vascular endothelial growth factor and vascular endothelial growth factor receptor-2 are associated with susceptibility to strok...


    pub_type: 杂志文章,多中心研究


    authors: Zhang W,Sun K,Zhen Y,Wang D,Wang Y,Chen J,Xu J,Hu FB,Hui R

    更新日期:2009-08-01 00:00:00

  • Mevastatin, an HMG-CoA reductase inhibitor, reduces stroke damage and upregulates endothelial nitric oxide synthase in mice.

    abstract:BACKGROUND AND PURPOSE:The 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) lower serum cholesterol and decrease the incidence of stroke and cardiovascular disease. There is growing evidence that statins exert some of their beneficial effects independent of cholesterol lowering. Indeed, we...


    pub_type: 杂志文章


    authors: Amin-Hanjani S,Stagliano NE,Yamada M,Huang PL,Liao JK,Moskowitz MA

    更新日期:2001-04-01 00:00:00

  • Evidence for platelet-activating factor as a novel mediator in experimental stroke in rabbits.

    abstract::Platelet-activating factor is a potent mediator of inflammation, which has untoward effects on cerebrovascular and neural elements. While several investigators have reported attenuation of ischemic damage after treatment with antagonists of platelet-activating factor, no study has proved endogenous production of plate...


    pub_type: 杂志文章


    authors: Lindsberg PJ,Yue TL,Frerichs KU,Hallenbeck JM,Feuerstein G

    更新日期:1990-10-01 00:00:00

  • Intravoxel Incoherent Motion Imaging in Small Vessel Disease: Microstructural Integrity and Microvascular Perfusion Related to Cognition.

    abstract:BACKGROUND AND PURPOSE:Cerebral small vessel disease (SVD) is associated with cognitive impairment. This may be because of decreased microstructural integrity and microvascular perfusion, but data on these relationships are scarce. We determined the relationship between cognition and microvascular perfusion and microst...


    pub_type: 杂志文章


    authors: Zhang CE,Wong SM,Uiterwijk R,Staals J,Backes WH,Hoff EI,Schreuder T,Jeukens CR,Jansen JF,van Oostenbrugge RJ

    更新日期:2017-03-01 00:00:00

  • Sonothrombolysis with transcranial color-coded sonography and recombinant tissue-type plasminogen activator in acute middle cerebral artery main stem occlusion: results from a randomized study.

    abstract:BACKGROUND AND PURPOSE:Sonothrombolysis is a new treatment approach in acute ischemic stroke. The results of a monocenter, randomized clinical study are presented. METHODS:Subjects with acute middle cerebral artery main stem occlusion were randomized into a target group receiving 1-hour transcranial continuous insonat...


    pub_type: 杂志文章,随机对照试验


    authors: Eggers J,König IR,Koch B,Händler G,Seidel G

    更新日期:2008-05-01 00:00:00