Abstract：
:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with discrete splicing complexes, and by their differing factor requirements. An early U1 snRNP-dependent cross-link of the branch region to a p80 species was followed by cross-links to p14, p35, and p150 polypeptides associated with the U2 snRNP-pre-mRNA complex. Concomitant with formation of the spliceosome, a rearrangement of protein factors about the branch region occurred, in which the p35 and p150 cross-links were replaced by p220 and p70 species. These results establish that the branch region is recognized in a dynamic fashion by multiple distinct proteins during the course of spliceosomal assembly.
journal_name
Genes Devjournal_title
Genes & developmentauthors
MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PAdoi
10.1101/gad.8.24.3008subject
Has Abstractpub_date
1994-12-15 00:00:00pages
3008-20issue
24eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract：:Outward migration of epidermal progenitors occurs with induction of hundreds of differentiation genes, but the identities of all regulators required for this process are unknown. We used laser capture microdissection followed by RNA sequencing to identify calmodulin-like 5 (CALML5) as the most enriched gene in differe...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.267708.115
更新日期：2015-11-01 00:00:00
abstract：:The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association ...
journal_title：Genes & development
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1101/gad.11.21.2926
更新日期：1997-11-01 00:00:00
abstract：:Chromatin is organized into higher-order structures that form subcompartments in interphase nuclei. Different categories of specialized enzymes act on chromatin and regulate its compaction and biophysical characteristics in response to physiological conditions. We present an overview of the function of chromatin struc...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.307702.117
更新日期：2017-11-15 00:00:00
abstract：:Cytosolic RNA/DNA sensing elicits primary defense against viral pathogens. Interferon regulatory factor 3 (IRF3), a key signal mediator/transcriptional factor of the antiviral-sensing pathway, is indispensible for interferon production and antiviral defense. However, how the status of IRF3 activation is controlled rem...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.277533.116
更新日期：2016-05-01 00:00:00
abstract：:Ste5, the prototypic mitogen-activated protein kinase (MAPK) scaffold protein, associates with plasma membrane-tethered Gbetagamma freed upon pheromone receptor occupancy, thereby initiating downstream signaling. We demonstrate that this interaction and membrane binding of an N-terminal amphipathic alpha-helix (PM mot...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1413706
更新日期：2006-07-15 00:00:00
abstract：:The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.12.1519
更新日期：1999-06-15 00:00:00
abstract：:One of the first steps in neurogenesis is the diversification of cells along the dorsoventral axis. In Drosophila the central nervous system develops from three longitudinal columns of cells: ventral cells that express the vnd/nk2 homeobox gene, intermediate cells, and dorsal cells that express the msh homeobox gene. ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.12.22.3591
更新日期：1998-11-15 00:00:00
abstract：:Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on th...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1296305
更新日期：2005-07-01 00:00:00
abstract：:Mammalian development requires effective mechanisms to repress genes whose expression would generate inappropriately specified cells. The Polycomb-repressive complex 1 (PRC1) family complexes are central to maintaining this repression. These include a set of canonical PRC1 complexes, each of which contains four core p...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.326488.119
更新日期：2019-07-01 00:00:00
abstract：:The dihydrofolate reductase (DHFR) and 2BE2121 genes in the Chinese hamster are convergently transcribed in late G1 and ea ly S phase, and bracket an early-firing origin of replication that consists of a 55-kb zone of potential initiation sites. To test whether transcription through the DHFR gene is required to activa...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1171404
更新日期：2004-02-15 00:00:00
abstract：:The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its sp...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1519507
更新日期：2007-04-01 00:00:00
abstract：:Mesoderm induction assays in Xenopus have implicated growth factors such as activin, Vg1, Xwnt-8, and noggin as important in directing the formation of dorsal mesoderm (Spemann's organizer). Because these growth factors are structurally very different, they presumably act through distinct cell surface receptors that i...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.24.3038
更新日期：1995-12-15 00:00:00
abstract：:cappuccino and spire are unique Drosophila maternal-effect loci that participate in pattern formation in both the anteroposterior and dorsoventral axes of the early embryo. Mutant females produce embryos lacking pole cells, polar granules, and normal abdominal segmentation. They share these defects with the posterior ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.3.9.1437
更新日期：1989-09-01 00:00:00
abstract：:Transgenic mice expressing wild-type murine p53 under the control of the mouse mammary tumor virus long terminal repeat (MMTV LTR) undergo progressive renal failure due to abnormal kidney development. Similar phenotypes are observed in two transgenic lines that express wild-type p53 within the ureteric bud but not in ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.7.836
更新日期：1996-04-01 00:00:00
abstract：:Viable aristaless (al) mutations of Drosophila affect pattern elements at both ends of the proximodistal axis in a subset of adult appendages. The al gene has been cloned and identified by P-element-mediated germ-line transformation with a genomic DNA fragment, which rescues a lethal mutation of al as well as aspects ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.1.114
更新日期：1993-01-01 00:00:00
abstract：:The superoxide radical (O(2)(-)) has long been considered a major cause of aging. O(2)(-) in cytosolic, extracellular, and mitochondrial pools is detoxified by dedicated superoxide dismutase (SOD) isoforms. We tested the impact of each SOD isoform in Caenorhabditis elegans by manipulating its five sod genes and saw no...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.504808
更新日期：2008-12-01 00:00:00
abstract：:The progressive bone marrow failure syndrome dyskeratosis congenita (DC) is often caused by mutations in telomerase or the factors involved in telomerase biogenesis and trafficking. However, a subset of DC patients is heterozygous for mutations in the shelterin component TIN2. To determine how the TIN2-DC mutations af...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.233395.113
更新日期：2014-01-15 00:00:00
abstract：:In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.11.1422
更新日期：1997-06-01 00:00:00
abstract：:Janus kinase 2 (JAK2) is a central kinase in hematopoietic stem/progenitor cells (HSPCs), and its uncontrolled activation is a prominent oncogenic driver of hematopoietic neoplasms. However, molecular mechanisms underlying the regulation of JAK2 have remained elusive. Here we report that the Casitas B-cell lymphoma (C...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.297135.117
更新日期：2017-05-15 00:00:00
abstract：:In the Bar mutation of Drosophila, ommatidial differentiation is known to be suppressed in the anterior portion of the eye. Our structural analysis shows that the Bar region contains a pair of homeo box genes, BarH1 and BarH2. These genes encode polypeptides similar in size and sequence and share a common homeo domain...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.1.50
更新日期：1992-01-01 00:00:00
abstract：:The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.417707
更新日期：2007-05-01 00:00:00
abstract：:To investigate the relationship between splicing and polyadenylation during the production of vertebrate mRNAs, we examined the effect of mutation of a poly(A) site on splicing of upstream introns. Mutation of the AAUAAA polyadenylation consensus sequence inhibited in vitro splicing of an upstream intron. The magnitud...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.11.2086
更新日期：1991-11-01 00:00:00
abstract：:Nitric oxide (NO) is an important second messenger involved in numerous biological processes, but how it regulates gene expression is not well understood. In this issue of Genes & Development, Cáceres and colleagues (pp. 1476-1485) report a critical requirement of NO as a direct regulator of gene expression through it...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.2080411
更新日期：2011-07-15 00:00:00
abstract：:PIWI clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ∼23- to 30-nucleotide (nt) PIWI-interacting RNAs (piRNAs) that are processed from single-stranded precursor transcripts via two distinct pathways. Primary piRNAs are defined by the endonuclease Zu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.267252.115
更新日期：2015-08-15 00:00:00
abstract：:Accurate completion of replication relies on the ability of cells to activate error-free recombination-mediated DNA damage bypass at sites of perturbed replication. However, as anti-recombinase activities are also recruited to replication forks, how recombination-mediated damage bypass is enabled at replication stress...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.265629.115
更新日期：2015-10-01 00:00:00
abstract：:Subependymal nodules (SENs) and subependymal giant cell astrocytomas (SEGAs) are common brain lesions found in patients with tuberous sclerosis complex (TSC). These brain lesions present a mixed glioneuronal phenotype and have been hypothesized to originate from neural stem cells. However, this hypothesis has not been...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.16750211
更新日期：2011-08-01 00:00:00
abstract：:RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supp...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.2062911
更新日期：2011-06-01 00:00:00
abstract：:Sexual identity in Drosophila is determined by zygotic X-chromosome dose. Two potent indicators of X-chromosome dose are sisterless-a (sis-a) and sisterless-b (sis-b). Genetic analysis has shown that a diplo-X dose of these genes activates their regulatory target, the feminizing switch gene Sex-lethal (Sxl), whereas a...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.7.9.1688
更新日期：1993-09-01 00:00:00
abstract：:Signaling by the hormones brassinosteroid (BR) and gibberellin (GA) is critical to normal plant growth and development and is required for hypocotyl elongation in response to dark and elevated temperatures. Active BR signaling is essential for GA promotion of hypocotyl growth and suppresses the dwarf phenotype of GA m...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.243675.114
更新日期：2014-08-01 00:00:00
abstract：:A complex array of genetic factors regulates neuronal dendrite morphology. Epigenetic regulation of gene expression represents a plausible mechanism to control pathways responsible for specific dendritic arbor shapes. By studying the Drosophila dendritic arborization (da) neurons, we discovered a role of the double-br...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.239962.114
更新日期：2014-09-01 00:00:00
