Dynamic association of proteins with the pre-mRNA branch region.

Abstract:

:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with discrete splicing complexes, and by their differing factor requirements. An early U1 snRNP-dependent cross-link of the branch region to a p80 species was followed by cross-links to p14, p35, and p150 polypeptides associated with the U2 snRNP-pre-mRNA complex. Concomitant with formation of the spliceosome, a rearrangement of protein factors about the branch region occurred, in which the p35 and p150 cross-links were replaced by p220 and p70 species. These results establish that the branch region is recognized in a dynamic fashion by multiple distinct proteins during the course of spliceosomal assembly.

journal_name

Genes Dev

journal_title

Genes & development

authors

MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PA

doi

10.1101/gad.8.24.3008

subject

Has Abstract

pub_date

1994-12-15 00:00:00

pages

3008-20

issue

24

eissn

0890-9369

issn

1549-5477

journal_volume

8

pub_type

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