Dynamic association of proteins with the pre-mRNA branch region.


:The association of proteins with the branch site region during pre-mRNA splicing was probed using a novel methodology to site-specifically modify the pre-mRNA with the photo-reagent benzophenone. Three sets of proteins were distinguished by the kinetics of their associations with pre-mRNAs, by their association with discrete splicing complexes, and by their differing factor requirements. An early U1 snRNP-dependent cross-link of the branch region to a p80 species was followed by cross-links to p14, p35, and p150 polypeptides associated with the U2 snRNP-pre-mRNA complex. Concomitant with formation of the spliceosome, a rearrangement of protein factors about the branch region occurred, in which the p35 and p150 cross-links were replaced by p220 and p70 species. These results establish that the branch region is recognized in a dynamic fashion by multiple distinct proteins during the course of spliceosomal assembly.


Genes Dev


Genes & development


MacMillan AM,Query CC,Allerson CR,Chen S,Verdine GL,Sharp PA




Has Abstract


1994-12-15 00:00:00












  • Reduced skin tumor development in cyclin D1-deficient mice highlights the oncogenic ras pathway in vivo.

    abstract::Cyclin D1 is part of a cell cycle control node consistently deregulated in most human cancers. However, studies with cyclin D1-null mice indicate that it is dispensable for normal mouse development as well as cell growth in culture. Here, we provide evidence that ras-mediated tumorigenesis depends on signaling pathway...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Robles AI,Rodriguez-Puebla ML,Glick AB,Trempus C,Hansen L,Sicinski P,Tennant RW,Weinberg RA,Yuspa SH,Conti CJ

    更新日期:1998-08-15 00:00:00

  • Bacterial promoter architecture: subsite structure of UP elements and interactions with the carboxy-terminal domain of the RNA polymerase alpha subunit.

    abstract::We demonstrate here that the previously described bacterial promoter upstream element (UP element) consists of two distinct subsites, each of which, by itself, can bind the RNA polymerase holoenzyme alpha subunit carboxy-terminal domain (RNAP alphaCTD) and stimulate transcription. Using binding-site-selection experime...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Estrem ST,Ross W,Gaal T,Chen ZW,Niu W,Ebright RH,Gourse RL

    更新日期:1999-08-15 00:00:00

  • Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.

    abstract::In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to the heptameric Sm ring for the assembly of the ring complex on pre-snRNAs at the conserved Sm site [A(U)4-6G]. Gemin5, a WD40 protein component of the SMN complex, is responsible for recognizing pre-snRNAs. In addi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Xu C,Ishikawa H,Izumikawa K,Li L,He H,Nobe Y,Yamauchi Y,Shahjee HM,Wu XH,Yu YT,Isobe T,Takahashi N,Min J

    更新日期:2016-11-01 00:00:00

  • Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.

    abstract::The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- an...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Szymczyna BR,Bowman J,McCracken S,Pineda-Lucena A,Lu Y,Cox B,Lambermon M,Graveley BR,Arrowsmith CH,Blencowe BJ

    更新日期:2003-02-15 00:00:00

  • A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase.

    abstract::Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that re...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Nishiyama A,Tachibana K,Igarashi Y,Yasuda H,Tanahashi N,Tanaka K,Ohsumi K,Kishimoto T

    更新日期:2000-09-15 00:00:00

  • The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways.

    abstract::We showed previously that the MAP kinase ERK2 is essential for aggregation. erk2 null cells lack cAMP stimulation of adenylyl cyclase and thus cannot relay the cAMP chemotactic signal, although the cells chemotax to cAMP (Segall et al. 1995). In this paper we have examined the role of ERK2 in controlling developmental...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gaskins C,Clark AM,Aubry L,Segall JE,Firtel RA

    更新日期:1996-01-01 00:00:00

  • Migrations of the Caenorhabditis elegans HSNs are regulated by egl-43, a gene encoding two zinc finger proteins.

    abstract::During embryonic development, the two Caenorhabditis elegans HSN motor neurons migrate from their birthplace in the tail to positions near the middle of the embryo. Here, we demonstrate that of all cells that undergo long-range migrations, only the HSNs are affected in animals that lack function of the egl-43 gene. We...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Garriga G,Guenther C,Horvitz HR

    更新日期:1993-11-01 00:00:00

  • Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1.

    abstract::Recruitment of p300/CBP by the hypoxia-inducible factor, HIF-1, is essential for the transcriptional response to hypoxia and requires an interaction between the p300/CBP CH1 region and HIF-1alpha. A new p300-CH1 interacting protein, p35srj, has been identified and cloned. p35srj is an alternatively spliced isoform of ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Bhattacharya S,Michels CL,Leung MK,Arany ZP,Kung AL,Livingston DM

    更新日期:1999-01-01 00:00:00

  • Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications.

    abstract::Genome integrity is constantly monitored by sophisticated cellular networks, collectively termed the DNA damage response (DDR). A common feature of DDR proteins is their mobilization in response to genotoxic stress. Here, we outline how the development of various complementary methodologies has provided valuable insig...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Polo SE,Jackson SP

    更新日期:2011-03-01 00:00:00

  • Checkpoint activation regulates mutagenic translesion synthesis.

    abstract::Cells have evolved checkpoint responses to arrest or delay the cell cycle, activate DNA repair networks, or induce apoptosis after genomic perturbation. Cells have also evolved the translesion synthesis processes to tolerate genomic lesions by either error-free or error-prone repair. Here, we show that after a replica...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kai M,Wang TS

    更新日期:2003-01-01 00:00:00

  • Genes within genes: independent expression of phage T4 intron open reading frames and the genes in which they reside.

    abstract::The td, nrdB, and sunY introns of bacteriophage T4 each contain a long open reading frame (ORF). These ORFs are preceded by functional T4 late promoters and, in the case of the nrdB intron ORF, a functional middle promoter. Expression of phage-encoded intron ORF-lacZ fusions indicates that these T4 genes are highly re...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Gott JM,Zeeh A,Bell-Pedersen D,Ehrenman K,Belfort M,Shub DA

    更新日期:1988-12-01 00:00:00

  • Time to move the fat.

    abstract::In this issue of Genes & Development, Dowen and colleagues (pp. 1515-1528) elegantly unify two previously unconnected aspects of physiology. The investigators provide significant genetic evidence to support a critical link between developmental timing decisions and the regulation of lipid mobilization at the transitio...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Weaver BP,Sewell AK,Han M

    更新日期:2016-07-01 00:00:00

  • Fibroblast growth factor signals regulate a wave of Hedgehog activation that is essential for coronary vascular development.

    abstract::Myocardial infarction and ischemic heart disease are the leading cause of death in the industrial world. Therapies employed for treating these diseases are aimed at promoting increased blood flow to cardiac tissue. Pharmacological induction of new coronary growth has recently been explored, however, clinical trials wi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Lavine KJ,White AC,Park C,Smith CS,Choi K,Long F,Hui CC,Ornitz DM

    更新日期:2006-06-15 00:00:00

  • Protein kinase A antagonizes Hedgehog signaling by regulating both the activator and repressor forms of Cubitus interruptus.

    abstract::The Hedgehog (Hh) family of secreted proteins controls many aspects of animal development. In Drosophila, Hh transduces its signal via Cubitus interruptus (Ci), a transcription factor present in two forms: a full-length activator and a carboxy-terminally truncated repressor that is derived from the full-length form by...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wang G,Wang B,Jiang J

    更新日期:1999-11-01 00:00:00

  • Hedgehog-regulated localization of Vax2 controls eye development.

    abstract::Cell fates in the optic neuroepithelium are determined by the combinational action of homeotic transcription factors. One of these is Vax2, a homeodomain protein that ventralizes the vertebrate eye field by repressing transcription of the Pax6 gene. We find that Vax2 shuttles between the nucleus and cytoplasm as a fun...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kim JW,Lemke G

    更新日期:2006-10-15 00:00:00

  • A master CLOCK hard at work brings rhythm to the transcriptome.

    abstract::In this issue of Genes & Development, Abruzzi et al. (pp. 2374-2386) use chromatin immunoprecipitation (ChIP) tiling array assays (ChIP-chip) to show that physical interactions between circadian (≅24-h) clock machineries and genomes are more widespread than previously thought and provide novel insights into how clocks...

    journal_title:Genes & development

    pub_type: 评论,杂志文章


    authors: Edery I

    更新日期:2011-11-15 00:00:00

  • A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.

    abstract::To investigate the importance of sequences between the yeast (Saccharomyces cerevisiae) branch point (TACTAAC box) and 3' splice site (AG), we generated a series of pre-mRNA substrates that differed in the length of RNA retained on the 3' side of the TACTAAC box. These pre-mRNAs were compared as substrates for the fir...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Rymond BC,Torrey DD,Rosbash M

    更新日期:1987-05-01 00:00:00

  • Regulated assembly and disassembly of the yeast telomerase quaternary complex.

    abstract::The enzyme telomerase, which elongates chromosome termini, is a critical factor in determining long-term cellular proliferation and tissue renewal. Hence, even small differences in telomerase levels can have substantial consequences for human health. In budding yeast, telomerase consists of the catalytic Est2 protein ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Tucey TM,Lundblad V

    更新日期:2014-10-01 00:00:00

  • An adenovirus early region 4 gene product is required for induction of the infection-specific form of cellular E2F activity.

    abstract::E2F is a cellular, sequence-specific DNA-binding factor that binds to pairs of sites that occur upstream of the E1A and E2 early mRNA cap sites. During adenovirus infection, there is induction of a form of E2F that binds cooperatively to the pair of sites in the E2 control region. Production of the infection-specific ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Hardy S,Engel DA,Shenk T

    更新日期:1989-07-01 00:00:00

  • MITF-the first 25 years.

    abstract::All transcription factors are equal, but some are more equal than others. In the 25 yr since the gene encoding the microphthalmia-associated transcription factor (MITF) was first isolated, MITF has emerged as a key coordinator of many aspects of melanocyte and melanoma biology. Like all transcription factors, MITF bin...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Goding CR,Arnheiter H

    更新日期:2019-08-01 00:00:00

  • Modulating the outer membrane with small RNAs.

    abstract::MicF, one of the first chromosomally encoded regulatory small RNAs (sRNAs) to be discovered, was found to modulate the expression of OmpF, an abundant outer membrane protein. Several recent papers have now shown that this is not an isolated case. At least five other sRNAs also regulate the synthesis of outer membrane ...

    journal_title:Genes & development

    pub_type: 杂志文章,评审


    authors: Guillier M,Gottesman S,Storz G

    更新日期:2006-09-01 00:00:00

  • Maternal western diet causes inflammatory milk and TLR2/4-dependent neonatal toxicity.

    abstract::For all newborn mammals, mother's milk is the perfect nourishment, crucial for their postnatal development. Here we report that, unexpectedly, maternal western diet consumption in mice causes the production of toxic milk that contains excessive long chain and saturated fatty acids, which triggers ceramide accumulation...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Du Y,Yang M,Lee S,Behrendt CL,Hooper LV,Saghatelian A,Wan Y

    更新日期:2012-06-15 00:00:00

  • The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

    abstract::Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Schatz PJ,Riggs PD,Jacq A,Fath MJ,Beckwith J

    更新日期:1989-07-01 00:00:00

  • Anti-diabetic rosiglitazone remodels the adipocyte transcriptome by redistributing transcription to PPARγ-driven enhancers.

    abstract::Rosiglitazone (rosi) is a powerful insulin sensitizer, but serious toxicities have curtailed its widespread clinical use. Rosi functions as a high-affinity ligand for peroxisome proliferator-activated receptor γ (PPARγ), the adipocyte-predominant nuclear receptor (NR). The classic model, involving binding of ligand to...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Step SE,Lim HW,Marinis JM,Prokesch A,Steger DJ,You SH,Won KJ,Lazar MA

    更新日期:2014-05-01 00:00:00

  • Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast.

    abstract::S-phase cyclin-dependent kinase Cdc28-Clb5 (CDK-S) and Dbf4-dependent kinase Cdc7-Dbf4 (DDK) are highly conserved kinases well known for their roles in the initiation of DNA replication. CDK-S is also essential for initiation of meiotic recombination because it phosphorylates Ser30 of Mer2, a meiosis-specific double-s...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Wan L,Niu H,Futcher B,Zhang C,Shokat KM,Boulton SJ,Hollingsworth NM

    更新日期:2008-02-01 00:00:00

  • MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein.

    abstract::In enteric bacteria, the transcription factor σ(E) maintains membrane homeostasis by inducing synthesis of proteins involved in membrane repair and two small regulatory RNAs (sRNAs) that down-regulate synthesis of abundant membrane porins. Here, we describe the discovery of a third σ(E)-dependent sRNA, MicL (mRNA-inte...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Guo MS,Updegrove TB,Gogol EB,Shabalina SA,Gross CA,Storz G

    更新日期:2014-07-15 00:00:00

  • MVH in piRNA processing and gene silencing of retrotransposons.

    abstract::VASA is an evolutionarily conserved RNA helicase essential for germ cell development. The mouse PIWI family proteins MILI and MIWI2 are involved in production of Piwi-interacting RNAs (piRNAs) in fetal male germ cells through a ping-pong amplification cycle. Expression of retrotransposons is elevated in MILI- and MIWI...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kuramochi-Miyagawa S,Watanabe T,Gotoh K,Takamatsu K,Chuma S,Kojima-Kita K,Shiromoto Y,Asada N,Toyoda A,Fujiyama A,Totoki Y,Shibata T,Kimura T,Nakatsuji N,Noce T,Sasaki H,Nakano T

    更新日期:2010-05-01 00:00:00

  • Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila.

    abstract::The third-chromosome mutation Killer of prune (K-pn) causes no phenotype by itself, but causes lethality in individuals homozygous for the nonlethal X-chromosome mutation prune (pn). We have recovered 12 gamma-ray-induced revertants of Killer of prune. All of the revertants fail to complement a recessive cell lethal m...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Biggs J,Tripoulas N,Hersperger E,Dearolf C,Shearn A

    更新日期:1988-10-01 00:00:00

  • BRCA1/BARD1 inhibition of mRNA 3' processing involves targeted degradation of RNA polymerase II.

    abstract::Mammalian cells exhibit a complex response to DNA damage. The tumor suppressor BRCA1 and associated protein BARD1 are thought to play an important role in this response, and our previous work demonstrated that this includes transient inhibition of the pre-mRNA 3' processing machinery. Here we provide evidence that thi...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Kleiman FE,Wu-Baer F,Fonseca D,Kaneko S,Baer R,Manley JL

    更新日期:2005-05-15 00:00:00

  • On the role of homologous sequences in chromosomal rearrangements.

    abstract::Deletions and other chromosomal rearrangements can be generated by recombination between repeated sequences. It has been shown in a number of systems that the probability of exchange or gene conversion decreases with increasing distance between repeats. This paper examines the question of how repeats find each other, ...

    journal_title:Genes & development

    pub_type: 杂志文章


    authors: Singer BS

    更新日期:1988-12-01 00:00:00