Abstract:
:A hairy-cell leukaemia (HCL) cell line, HCL-O, was established from the peripheral blood of a 62-year-old Japanese patient with a unique variant of HCL strongly expressing CD21, the receptor for the Epstein-Barr virus (EBV). The HCL-O cells expressed antigens similar and dissimilar to those expressed with the original hairy cells. The HCL-O cells were more mature than the original cells in their degree of B-cell differentiation, as indicated by a decrease of CD19 and surface immunoglobulin (sIg) expression together with the appearance of CD38 and cytoplasmic Ig (cIg). In addition, the cells expressed CD11c recognized by Leu-M5, a monoclonal antibody usually positive for HCL. Their karyotype and Ig gene rearrangement pattern were identical to those of the original cells. The EBV genome was detected in the HCL-O cells but not in the original cells. The HCL-O cells spontaneously produced a large quantity of interleukin-6 (IL-6) in the conditioned medium, whereas IL-6 serum level was not so high. These findings indicate that the HCL-O cell line is derived from the leukaemic hairy cells and possibly, in vitro EBV infection took place easily in the original hairy cells through their CD21, resulting in subsequent immortalization. IL-6 production by HCL-O cells may be induced or enhanced by EBV, and the secreted IL-6 might play a role in their own growth or differentiation.
journal_name
Acta Haematoljournal_title
Acta haematologicaauthors
Tokioka T,Shimamoto Y,Nagumo F,Tadano J,Yamaguchi Mdoi
10.1159/000204129subject
Has Abstractpub_date
1994-01-01 00:00:00pages
8-13issue
1eissn
0001-5792issn
1421-9662journal_volume
92pub_type
杂志文章abstract::To clarify the role of thrombopoietin (c-Mpl ligand, TPO) in 'hypersplenic' thrombocytopenia, we used an enzyme-linked immunosorbent assay to examine changes in serum TPO levels accompanied with splenectomy in 6 patients with liver cirrhosis, 4 patients with gastric cancer, and 2 patients with lymphoid malignancies. W...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040888
更新日期:1998-12-01 00:00:00
abstract:BACKGROUND:It is a common belief among women that iron compounds have unpleasant gastrointestinal side effects. OBJECTIVE:To assess the gastrointestinal side effects of iron prophylaxis in pregnancy. METHODS:A randomized, double-blind study comprising 404 healthy pregnant women allocated to four groups taking ferrous...
journal_title:Acta haematologica
pub_type: 杂志文章,随机对照试验
doi:10.1159/000089466
更新日期:2006-01-01 00:00:00
abstract::We have been studying hematopoietic effects by the tachykinins, which like many other neuropeptides can be expressed in neural and nonneural tissues. Substance P (SP) and neurokinin-A (NK-A), members of the tachykinins are immune and hematopoietic modulators. SP and NK-A are derived from the preprotachykinin-I gene (P...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000203593
更新日期:1997-01-01 00:00:00
abstract::The short-term effect of a domestically produced equine antithymocyte globulin (ATG) was analyzed in 6 patients with acquired severe aplastic anemia (AA). All patients received 5 doses of ATG every other day in a 60-min intravenous infusion. Five peripheral blood immunoregulatory mononuclear cell (MNC) subsets, define...
journal_title:Acta haematologica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000205557
更新日期:1989-01-01 00:00:00
abstract::A 16-year-old Japanese woman with acute myelogenous leukemia (AML) and t(6;9) received peripheral blood stem cell transplantation (PBSCT). Although chromosomal studies just prior to and following PBSCT showed a normal karyotype, reverse transcription-polymerase chain reaction (RT-PCR) detected the mRNA derived from th...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203996
更新日期:1995-01-01 00:00:00
abstract::Serum soluble interleukin-6 receptor (sIL-6R) concentrations were measured in 50 patients with plasma cell dyscrasias using a commercially available immunoenzymatic assay kit. There were 40 patients with multiple myeloma (MM), 5 patients with monoclonal gammopathy of undetermined significance (MGUS), 3 patients with s...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203682
更新日期:1997-01-01 00:00:00
abstract::The maturation process of leukemic myelomonocytoid cells derived from peripheral blood of two typical cases of chronic myelomonocytic leukemia was investigated in vitro. In both cases, liquid culture of the leukemic cells in the presence or absence of 12-o-tetradecanoyl phorbol-13-acetate yielded either monocyte-macro...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206382
更新日期:1984-01-01 00:00:00
abstract::Monosomy 7 myelodysplasia is a rare hematological entity and is associated with morphological abnormalities in bone marrow and peripheral smear, and poor prognosis in children. We describe 2 children with infantile monosomy 7 myelodysplasia which evolved to leukemia. One of them died after 1 month, and the other is st...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204135
更新日期:1994-01-01 00:00:00
abstract::RAS mutations are found in about 25% of acute myeloid leukemia (AML) cases. The importance of these changes is unknown. If RAS mutations confer growth advantage to leukemia subclones in which they emerge, substantially more nonconservative than conservative mutations should be found. The incidence of conservative muta...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204200
更新日期:1994-01-01 00:00:00
abstract::We evaluated the results of high-dose therapy (HDT) and autologous hematopoietic stem cell transplantation (ASCT) in patients with relapsed or primary refractory Hodgkin's disease (HD), using a previously reported prognostic model based on the presence of three poor prognostic factors at the start of salvage therapy/p...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000074220
更新日期:2003-01-01 00:00:00
abstract::Significant DNA polymorphisms have been reported in the beta-globin gene cluster of epsilon-G gamma-A gamma-psi beta-delta-beta-gene region, in normal (Hb AA) individuals and in patients with sickle cell anaemia (SCA). Investigations of the extent of the DNA polymorphisms in the beta A- and beta S-globin gene cluster ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204653
更新日期:1992-01-01 00:00:00
abstract::We have retrospectively analyzed a series of 19 patients with hepatitis C virus (HCV) infection and chronic thrombocytopenia not attributable to hypersplenism or to other causes. Antiplatelet antibodies were present in 81% of cases. Response to prednisone was observed in 6 of 7 patients and 1 of 3 patients responded t...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040842
更新日期:1998-01-01 00:00:00
abstract::A 67-year-old woman with agnogenic myeloid metaplasia, who underwent splenectomy 1 year after the diagnosis, experienced spontaneous hematologic remission 6 years after splenectomy. Ten months before her anemia improved, peripheral leukocytes no longer showed a shift to the left and peripheral erythroblasts had disapp...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205163
更新日期:1990-01-01 00:00:00
abstract::Hypercalcemia in malignancies is a frequent complication, mostly affecting patients with solid tumors or multiple myeloma. Calcium elevation is induced by direct bone infiltration of a tumor mass or through calcium liberation from the skeleton by a humoral mediator. The latter mechanism is referred to as humoral hyper...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000314646
更新日期:2010-01-01 00:00:00
abstract::Iron deficiency is the main cause of anemia in both sexes, with women being more commonly affected. Iron therapy is currently considered an effective and safe remedy to replenish the iron storages. Iron can be administrated both orally and intravenously. In particular, intravenous (IV) iron therapy is widely used when...
journal_title:Acta haematologica
pub_type:
doi:10.1159/000503768
更新日期:2020-01-01 00:00:00
abstract::Bone marrow samples of 28 individuals with clinically benign and of 41 patients with malignant monoclonal gammopathy were analyzed for the total number of lymphoplasmocellular elements containing cytoplasmic immunoglobulins and for the monoclonal fraction of these cells. Monoclonal immunoglobulin components were deter...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207709
更新日期:1978-01-01 00:00:00
abstract::An improved plasma clot culture method for CFU megakaryocytes (MK) has been developed with a higher plating efficiency and easier identification and enumeration of MK colonies by an indirect immunoperoxidase staining using a monoclonal antibody specific to the platelet glycoprotein IIb/IIIa complex. This technique has...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205837
更新日期:1987-01-01 00:00:00
abstract::We evaluated the iron status of 50 Sicilian patients with G6PD deficiency under steady-state conditions and compared our results with those for 50 control patients. We studied haemolysis and iron indices to evaluate the iron balance. These patients could be considered to be at risk of iron overload as a result of incr...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204368
更新日期:1993-01-01 00:00:00
abstract::Two pregnancies at risk for Fanconi's anemia have been monitored by a cytogenetic method in the first trimester of gestation. The rate of chromosome breakage was evaluated in spontaneous mitoses from a direct preparation of trophoblasts in one case and from mitoses obtained from standard cultures and from mitoses trea...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206309
更新日期:1985-01-01 00:00:00
abstract::A case of acute leukaemia with t(4;11) chromosomal abnormality in a 28-year-old woman is reported. At diagnosis, two blast cell populations were seen: 60% of the cells were small cells with lymphoid morphology, 40% were large cells with monocytic morphology. Cytochemical examination was consistent with acute myeloid l...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206046
更新日期:1986-01-01 00:00:00
abstract::A modified cytochemical method was developed for demonstrating magnesium-activated adenosine triphosphatase in haemic cells. The enzyme was shown in a variety of normal and leukaemic, unfixed cells. The high sensitivity of this enzyme to all fixatives tried in this study hampered further exploration and characterizati...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205983
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Splanchnic vein thrombosis (SVT) is a severe complication of essential thrombocythemia (ET). No clear explanation has been given for the occurrence of thrombosis in this unusual site in patients with ET, but the existence of a specific association between unexplained SVT and the JAK2 mutation has been report...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000221962
更新日期:2009-01-01 00:00:00
abstract::A 77-year-old woman presented to the outpatient hematology clinic in August 2001 with leukocytosis, recurrent bacterial infections, sweating and weight loss. Bone marrow biopsy showed 80% infiltration with lymphoid cells having a prolymphocytic morphology. Flow-cytometric immunophenotype analysis showed that over 80% ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000076527
更新日期:2004-01-01 00:00:00
abstract::Systematic reviews and meta-analyses have made a major contribution to the evidence-based management of infections in cancer patients. We review the contribution of systematic reviews with regard to antibiotic, antifungal, antiviral and Pneumocystis pneumonia prophylaxis; antibiotic and antifungal treatment of febrile...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000318900
更新日期:2011-01-01 00:00:00
abstract::Fetal hemoglobin (HbF) concentrations were measured by a radial immunodiffusion assay in 233 patients with various malignancies. In 96 of these, alpha-fetoprotein (AFP) was also measured by radioimmunoassay. The concentration of HbF exceeded 2 SDs above the normal mean in 39 of 233 patients, most notably in patients w...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207839
更新日期:1977-01-01 00:00:00
abstract::Patients with HIV-associated lymphocyte-depleted Hodgkin lymphoma (HIV-HL) often present with advanced, extranodal disease and aggressive clinical features, limiting definitive therapeutic intervention. Here we report two patients with HIV-HL who presented with multi-organ dysfunction as an initial manifestation of th...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000500222
更新日期:2020-01-01 00:00:00
abstract::A study of E-rosette ultrathin sections has been undertaken in an attempt to analyze changes induced during the T-lymphocyte-sheep red blood cell interaction on the rosetted lymphocyte surface architecture. The formation of nonimmune rosettes implies different types of attachment between lymphocytes and sheep red bloo...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207570
更新日期:1979-01-01 00:00:00
abstract::A 3-year-old child developed severe aplastic anaemia following hepatitis A. Since no HLA-compatible donor was available, he was treated with oxymetholone, antithymocytic globulin and methylprednisolone, but no haematologic recovery was observed and he consequently died of pneumonia. Although the association of aplasti...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206061
更新日期:1986-01-01 00:00:00
abstract::We describe a case of hemolytic anemia with subacute bacterial endocarditis due to Streptococcus sanguis. The major hematological features of the patient were a normocytic anemia with reticulocytosis, an elevation of serum lactate dehydrogenase, and a marked reduction of serum haptoglobin. All these signs of hemolytic...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203981
更新日期:1995-01-01 00:00:00
abstract::A 69-year-old woman with typical thrombotic thrombocytopenic purpura is reported, who was treated successfully by plasma exchange. An initial chromosomal analysis of peripheral blood lymphocytes revealed abnormal patterns of 45, XO/46, XX/47, XXX; a second analysis showed 45, XO/46, XX/47, XXX/47, XX + 21, and a third...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205068
更新日期:1990-01-01 00:00:00