Abstract:
:Although investigations to date have demonstrated the ability of the monocyte/macrophage to synthesize complement components, only a limited number of studies on complement synthesis by nonhepatic tissue cells have been reported. To begin to fill this gap in our knowledge we have recently evaluated the ability of lung tissue cells to synthesize and secrete various complement components in vitro. Using 35S-methionine incorporation and immunoprecipitation techniques we have previously demonstrated the ability human lung type II pneumocytes (A549) and human lung fibroblasts (WI-38), to synthesize and secrete a variety of both early and terminal complement components, as well as several regulatory proteins including Clr, Cls, C4, C3, C5, C6, C7, C8, C9, Factor B, Factor H, Factor I and Cls inactivator. Our present studies demonstrate the capability of silica to regulate complement component production by A549 cells, but not complement component production by WI-38 cells. Specifically, using sensitive ELISAs we demonstrated that a non-toxic dose of silica had the capability to suppress the production of both C3 and C5 by A549 pneumocytes by 40-50 percent, but had no effect on C3 or C5 synthesis by WI-38 fibroblasts. Additionally, using 35S-methionine incorporation and TCA precipitation techniques, we demonstrated that suppression of C3 and C5 production by silica treated A549 pneumocytes was not a result of suppression of total protein synthesis. These studies demonstrate that silica, which has been implicated in pulmonary diseases, has the capability to regulate local complement production by lung tissue cells in vitro. In vivo, this suppression of complement production by the type II pneumocytes could alter the local tissue reservoir of complement components during infection and pulmonary injury, thus resulting in depressed pulmonary host defense.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Rothman BL,Contrino J,Merrow M,Despins A,Kennedy T,Kreutzer DLdoi
10.3109/08923979409019738subject
Has Abstractpub_date
1994-11-01 00:00:00pages
525-51issue
4eissn
0892-3973issn
1532-2513journal_volume
16pub_type
杂志文章abstract::Paraquat (methyl viologen, PQ) is highly toxic to humans. Pulmonary fibrosis is the most common cause of death after PQ poisoning. However, no effective therapy is available. The current treatment dilemma and pathology suggest that we should reconsider how to treat the poisoning using other methods, such as immunizati...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2018.1490319
更新日期:2018-08-01 00:00:00
abstract::Traditionally venoms are used from thousands of years to treat pain, inflammation, and arthritis. In Ayurveda "Suchika Voron" and "Shodhona" were practiced against pain. In the present study, venom composition of the Indian honeybee Apis florea (AF), Apis dorsata (AD), and Apis cerana indica (AC) were analyzed using e...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1009996
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVE:Ipomoea obscura (L.) Ker-Gawl, is a medicinal herb with indole alkaloids as an active constituent. In this study, we investigated the anti-angiogenic activity of I. obscura extract and one of its major compounds Ipobscurine-A (IPO-A). METHODS:In vivo angiogenesis was induced by injecting B16F10 melanoma cell...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2010.531277
更新日期:2011-09-01 00:00:00
abstract::Context: Pyocyanin is a typical Pseudomonas aeruginosa virulence factor, a common Gram-negative rod responsible for a wide range of severe nosocomial infections. There is evidence indicating that pyocyanin has multiple biological activities, but little is known about anti-inflammatory properties. Objective: This study...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2018.1555845
更新日期:2019-02-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs), one of the major orchestrators of immunosuppressive network are present in the tumor microenvironment suppress antitumor immunity by subverting Th1 response in tumor site and considered as a great obstacle for advancement of different cancer immunotherapeutic protocols. Till d...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2014.897727
更新日期:2014-04-01 00:00:00
abstract::The popular murine macrophage cell line, RAW 264.7, is often used to initially screen natural products for bioactivity and to predict their potential effect in vivo or on primary cells. The cell line response is considered to reflect the potential human de novo response, and is used to evaluate the effective bioactivi...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2017.1282511
更新日期:2017-04-01 00:00:00
abstract::Plants have been shown to possess a number of beneficial anticancer and immunomodulatory properties. In this study the possible in vitro antitumor activity and immunomodulatory effects of five species of Euphorbia, an important genus of Euphorbiaceae, including E. petiolata, E. hebecarpa, E. osyridea, E. microciadia, ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923971003699018
更新日期:2011-03-01 00:00:00
abstract::Sera obtained from a group of pigs (n = 5) fed a diet amended with fumonisin containing Fusarium moniliforme culture material was used to determine the levels of Tumor Necrosis Factor-Alpha (TNF) activity by a functional bioassay utilizing the TNF sensitive WEHI 140 mouse fibrosarcoma cell line. Two pigs developed sig...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979709007663
更新日期:1997-05-01 00:00:00
abstract::Enhanced glucose utilization was observed in continuous cultures of macrophages in medium containing 4mM Quadrol, [N,N,N',N',Tetrakis(2-hydroxypropyl)ethylenediamine]. This enhancement was both time and concentration dependent. Radiolabeled 4mM Quadrol, after incubation with macrophages for 60 minutes, was shown to be...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978709035206
更新日期:1987-01-01 00:00:00
abstract::We investigated the effect of aqueous extract of Sanguisorba officinalis L.(Rosaceae) root (SOAE) on the immediate-type allergic reactions in vivo and in vitro. SOAE (0.01 to 1 g/kg) inhibited systemic allergic reaction induced by compound 48/80. When SOAE was employed in a systemic allergic reaction test, the plasma ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120014729
更新日期:2002-08-01 00:00:00
abstract::This study is to explore the inner anti-inflammatory mechanism of the ethanol extract of Rungia pectinata (Linn.) Nees. As a result, the ethanol extract of Rungia pectinata (Linn.) Nees could not only strongly reduce production of pro-inflammatory cytokines and mediators via blocking NF-kappaB activation but slightly ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970701812704
更新日期:2008-01-01 00:00:00
abstract::A polymerase chain reaction was devised to simultaneously detect repeated insertion sequences and the pertussis toxin promoter gene for the diagnostic identification of Bordetella pertussis, B. parapertussis, and B. bronchiseptica. The sensitivity of this method was sufficient to detect one B. pertussis organism using...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979909016394
更新日期:1999-02-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is a ubiquitously expressed ATP-dependent molecular chaperone across all species that helps to the correct the folding of many proteins related to important signaling pathways. Tumor cells expressing Hsp90 have more ATP-binding affinity than normal cells. Many correlative inhibitors have ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1119159
更新日期:2016-01-01 00:00:00
abstract::In the present study, we introduce a new class of organometallic compound, the Biphosphinic Palladacycle Complex [Pd (C2, N-S(-)(dmpa)(dppf)] Cl (BPC), as an angiotensin-I converting-enzyme inhibitor (ACEI) with hematological regulation properties. When BPC was assayed as a competitive inhibitor over the hydrolysis of...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-200042263
更新日期:2004-01-01 00:00:00
abstract::During the last few years, several observations outline that the impaired T lymphocyte proliferative capacity in the elderly is due to a reduced interleukin 2 (IL-2) release. To further investigate the activation process during lectin stimulation, aged peripheral blood mononuclear cells (PBMC) were stimulated with phy...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019693
更新日期:1991-01-01 00:00:00
abstract::Cancer is responsible for millions of deaths each year worldwide. Pharmacological intervention with plant-derived products alone or in combination to reverse, suppress, or prevent the cancer progression plays a key role in the fight against this terrible disease. Aerva lanata is an important medicinal plant widely use...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2010.526614
更新日期:2011-09-01 00:00:00
abstract::Serum tumor necrosis factor (TNF) levels in 33 patients with inflammatory bowel disease (IBD) were measured by using a sensitive enzyme immunoassay. Four of five Crohn's diseases (CD) and nine of twenty eight ulcerative colitis (UC) had elevated levels of serum TNF. In active CD or UC, a greater fraction of patients s...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979209005404
更新日期:1992-01-01 00:00:00
abstract::In the present study, intrahepatic CD8+ lymphocyte infiltrates as well as HLA class I and CD54 (ICAM-1) antigen expression at both tissue and serum levels were evaluated in 54 untreated patients with chronic hepatitis C stratified on the basis of histological diagnosis (Chronic Persistent Hepatitis/Chronic Lobular Hep...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979809034818
更新日期:1998-08-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.658922
更新日期:2012-10-01 00:00:00
abstract::A competitive enzyme-linked immunosorbent assay (ELISA) with generated mouse anti-consensus interferon (CIFN) antibody was developed for quantitative determination of pegylated consensus interferon (PEG-CIFN) in rhesus monkey serum. The operational concentrations of the original assay were determined using the chessbo...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970902814111
更新日期:2009-01-01 00:00:00
abstract::The effects of cimetidine upon suppressor cell effector function in a well-studied murine model of contact hypersensitivity were examined. Intravenous inoculation of BALB/c mice with DNP-coupled syngeneic spleen cells induced the production of DNP-specific suppressor cells which was demonstrated by a reduction in ear ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809041424
更新日期:1988-01-01 00:00:00
abstract::We examined the effect of sulfapyridine on mast cell-mediated immediate-type allergic reactions. Sulfapyridine (1 and 10 microg/kg) significantly inhibited systemic allergic reaction induced by compound 48/80 in rats. Sulfapyridine (1 and 10 microg/kg) also inhibited significantly local mast cell-mediated immediate-ty...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970009016419
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:Tacrolimus causes post-transplant diabetes mellitus, however the pathogenetic mechanisms remain controversial. In this study we probed into the mechanisms of tacrolimus-induced diabetes mellitus in rats. METHODS:Glucose levels were determined on whole blood samples using a glucose oxidase method. Levels of ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970903032747
更新日期:2009-01-01 00:00:00
abstract::The immunological status of lead acid battery workers with blood lead levels and urinary delta-aminolevulinic acid (ALA-U) concentrations ranging from safe to toxic levels has been examined and compared with those of non-exposed, age and sex matched controls. No differences in the serum concentrations of IgG, IgA and ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979409029904
更新日期:1994-02-01 00:00:00
abstract::Immunostimulants known to initiate cytokine production were found to inhibit processes of microsomal drug oxidation but to activate arylamine N-acetylation. The present study investigated the effects of immunstimulating doses of rat interferon-gamma (IFN gamma, 670,000 units ip) and streptolysin O (SLO, 100 HU/kg iv f...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979609052754
更新日期:1996-11-01 00:00:00
abstract::To probe into the mechanism of immunosuppression of FTY720, the authors study the cell cycle arrest effects of large-dose FTY720 on lymphocytes in mouse skin transplantation models and the expression of cell cycle-related factors in cell cycle regulation system in mouse skin allograft models using flow cytometry, Immu...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970801949174
更新日期:2008-01-01 00:00:00
abstract::Chronic administration of oxytetracycline (OXT) (incorporated at a level of 0.05 g per kg of feed for 50 days) to pigeons, significantly decreased total leukocyte and lymphocyte counts, increased heterophil:lymphocyte ratio and lysosomal enzyme activity, and decreased reticuloendothelial system function compared with ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970500242327
更新日期:2005-01-01 00:00:00
abstract:AIM:To determine the steady state concentration and the pharmacokinetics of Cyclosporine A (CsA) in heart transplant recipients. METHODS:A single dose of 100 mg CsA capsules twice daily was given to five heart transplant recipients of steady state. The concentrations of CsA in plasma were determined by streptavidin pe...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
doi:10.3109/08923973.2011.613400
更新日期:2012-06-01 00:00:00
abstract::We conducted a comparative study of NK1.1+ cells in spleen and bone marrow and the effects of administration of M-CSF on them. Administration of M-CSF to mice increased the number of NK1.1+ cells in spleen but not in bone marrow. The NK1.1+ cells in spleen (Spl-NK1.1) and bone marrow (BM-NK1.1) were purified by magnet...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120016035
更新日期:2002-11-01 00:00:00
abstract::3'-azido-3'-deoxythymidine (AZT) was administered orally to 8 AIDS patients at a dose of 100 mg every 6 hours for 14 days. On days 8 - 14 the patients were also given 1 g inosine-pranobex (INPX) every 6 hours. On day 7, while the subjects were taking AZT alone and on day 14 while they were receiving AZT + INPX, blood ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809006447
更新日期:1988-01-01 00:00:00