Discovering the mechanism of action of novel antibacterial agents through transcriptional profiling of conditional mutants.

abstract：:Botulism is caused by botulinum neurotoxin (BoNT), the most poisonous substance known. BoNTs are also classified as Tier 1 biothreat agents due to their high potency and lethality. The existence of seven BoNT serotypes (A-G), which differ between 35% to 68% in amino acid sequence, necessitates the development of serot...

journal_title：Antimicrobial agents and chemotherapy

authors： Snow DM,Riling K,Kimbler A,Espinoza Y,Wong D,Pham K,Martinez Z,Kraus CN,Conrad F,Garcia-Rodriguez C,Cobb RR,Marks JD,Tomic MT

更新日期：2019-10-07 00:00:00

abstract：:The activity of nalidixic acid combined with each of 10 other antimicrobial agents on 95 strains of Enterobacteriaceae was studied. Synergism was found less often than antagonism, and the commonest outcome was indifference. Combinations of nalidixic acid with kanamycin, gentamicin, or colistin were more often synergis...

journal_title：Antimicrobial agents and chemotherapy

authors： Michel J,Luboshitzky R,Sacks T

更新日期：1973-09-01 00:00:00

abstract：:The only human cathelicidin, the 37-residue peptide LL-37, exhibits antimicrobial activity against both gram-positive and gram-negative bacteria. We studied the ability of several fragments of LL-37, exhibiting different antimicrobial activities, to interact with membranes whose compositions mimic the cytoplasmic memb...

journal_title：Antimicrobial agents and chemotherapy

authors： Epand RF,Wang G,Berno B,Epand RM

更新日期：2009-09-01 00:00:00

abstract：:Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae isolates have been increasingly reported worldwide, and therapeutic options to treat infections caused by these organisms are limited. We evaluated the activity of ceftazidime-avibactam and comparators against 456 Enterobacteriaceae isolates carryi...

journal_title：Antimicrobial agents and chemotherapy

authors： Castanheira M,Mendes RE,Sader HS

更新日期：2017-02-23 00:00:00

abstract：:Mefloquine is widely used in combination with artemisinin derivatives for the treatment of falciparum malaria. Mefloquine resistance in Plasmodium falciparum has been related to increased copy numbers of multidrug-resistant gene 1 (pfmdr1). We studied the ex vivo dynamics of pfmdr1 gene amplification in culture-adapte...

journal_title：Antimicrobial agents and chemotherapy

authors： Preechapornkul P,Imwong M,Chotivanich K,Pongtavornpinyo W,Dondorp AM,Day NP,White NJ,Pukrittayakamee S

更新日期：2009-04-01 00:00:00

abstract：:The phosphodiesterase inhibitor tetrahydrophthalazinone NPD-008 was explored by phenotypic in vitro screening, target validation and ultrastructural approaches against Trypanosoma cruzi NPD-008 displayed activity against different forms and strains of T. cruzi (EC50 = 6.6 - 39.5 μM), increased cAMP levels of T. cruzi ...

journal_title：Antimicrobial agents and chemotherapy

authors： de Araújo JS,Peres RB,Bernardino da Silva P,Batista MM,Sterk GJ,Maes L,Caljon G,Leurs R,de Koning HP,Kalejaiye TD,Soeiro MNC

更新日期：2020-12-23 00:00:00

abstract：:Routine in vitro screening of a new synthetic series of 3,5-substituted 2-methylisoxazolidines revealed that three imidazole analogs (PR 967-248, PR 967-234, and PR 969-566) and, to a lesser extent, a triazole analog (PR 988-399) exerted rather potent antifungal activity against three systemic and four dermatophytic c...

journal_title：Antimicrobial agents and chemotherapy

authors： Palmer GC,Ordy MJ,Simmons RD,Strand JC,Radov LA,Mullen GB,Kinsolving CR,St Georgiev V,Mitchell JT,Allen SD

更新日期：1989-06-01 00:00:00

abstract：:We have used the endogenous reverse transcriptase reaction of viral core particles from duck liver to elucidate the mechanism of inhibition of duck hepatitis B virus (DHBV) replication by the nucleoside analog (-)-beta-L-2',3'-dideoxy-3'-thiacytidine (3TC). As is the case in human immunodeficiency virus replication, 3...

journal_title：Antimicrobial agents and chemotherapy

authors： Severini A,Liu XY,Wilson JS,Tyrrell DL

更新日期：1995-07-01 00:00:00

abstract：:A new, rapid method for measuring serum levels of nafcillin by spectrofluorometry is described. The method involves extraction of 2 ml of acidified serum with n-butyl chloride, subjecting the organic solvent layer to excitation at 340 nm, and measuring the relative intensity of emission fluorescence at 380 nm. An exce...

journal_title：Antimicrobial agents and chemotherapy

authors： Lichtenwalner DM,Suh B,Lorber B,Sugar AM

更新日期：1979-08-01 00:00:00

abstract：:Helicobacter pylori is killed in vitro by polyoxyethylene acyl esters and ethers similar to simethicone emulsifiers in therapeutic antifoams. The MBC of these compounds for Helicobacter pylori was less than 20 micrograms/ml, while other gram-negative bacteria were unaffected by much higher concentrations of up to 50 m...

journal_title：Antimicrobial agents and chemotherapy

authors： Kane AV,Plaut AG

更新日期：1996-02-01 00:00:00

abstract：:An experiment was conducted in animal facilities to compare the impacts of four avian colibacillosis treatments-oxytetracycline (OTC), trimethoprim-sulfadimethoxine (SXT), amoxicillin (AMX), or enrofloxacin (ENR)-on the susceptibility of Escherichia coli in broiler intestinal tracts. Birds were first orally inoculated...

journal_title：Antimicrobial agents and chemotherapy

authors： Dheilly A,Le Devendec L,Mourand G,Bouder A,Jouy E,Kempf I

更新日期：2012-01-01 00:00:00

abstract：:Replication of hepatitis C virus (HCV) RNA is catalyzed by the virally encoded RNA-dependent RNA polymerase NS5B. It is believed that the viral polymerase utilizes a de novo or primer-independent mechanism for initiation of RNA synthesis. Our previous work has shown that dinucleotides were efficient initiation molecul...

journal_title：Antimicrobial agents and chemotherapy

authors： Zhong W,An H,Barawkar D,Hong Z

更新日期：2003-08-01 00:00:00

abstract：:The effects on Staphylococcus aureus viability and beta-lactamase activity of concentrations that simulated those in human serum after a combined dose of 875 mg of amoxicillin and 125 mg of clavulanic acid were studied in an in vitro pharmacodynamic model. Six hours of preexposure to concentrations of the amoxicillin-...

journal_title：Antimicrobial agents and chemotherapy

authors： Aguilar L,Martín M,Balcabao IP,Gómez-Lus ML,Dal-Ré R,Prieto J

更新日期：1997-06-01 00:00:00

abstract：:The creation of new chemotherapeutic regimens that permit shortening the duration of treatment is a major priority for antituberculosis drug development. In this study, we used the murine model of experimental tuberculosis therapy to determine whether incorporation of the investigational new nitroimidazopyran PA-824 i...

journal_title：Antimicrobial agents and chemotherapy

authors： Nuermberger E,Rosenthal I,Tyagi S,Williams KN,Almeida D,Peloquin CA,Bishai WR,Grosset JH

更新日期：2006-08-01 00:00:00

abstract：:The in vitro susceptibilities of 22 penicillinase-producing and 32 non-penicillinase-producing strains of Neisseria gonorrhoeae to 13 antimicrobial agents, including the new semisynthetic penicillins and cephalosporins, are reported. Ceftriaxone, ceftazidime, and cefotaxime were the most active agents tested; none of ...

journal_title：Antimicrobial agents and chemotherapy

authors： Coovadia YM,Ramsaroop U

更新日期：1984-11-01 00:00:00

abstract：:The rpoB gene encodes the beta subunit of the DNA-dependent RNA polymerase of bacteria. Mutations in defined areas result in resistance to rifampin. Mycobacterium smegmatis is naturally resistant to rifampin, but analysis of the rpoB gene revealed no identifiable rifampin resistance mutations. Another mechanism of res...

journal_title：Antimicrobial agents and chemotherapy

authors： Hetherington SV,Watson AS,Patrick CC

更新日期：1995-09-01 00:00:00

abstract：:We and others recently identified copper resistance as important for virulence of Mycobacterium tuberculosis. Here, we introduce a high-throughput screening assay for agents that induce a copper hypersensitivity phenotype in M. tuberculosis and demonstrate that such copper-boosting compounds are effective against repl...

journal_title：Antimicrobial agents and chemotherapy

authors： Speer A,Shrestha TB,Bossmann SH,Basaraba RJ,Harber GJ,Michalek SM,Niederweis M,Kutsch O,Wolschendorf F

更新日期：2013-02-01 00:00:00

abstract：:Seventy nine strains of Yersinia enterocolitica resistant to one or more antimicrobials were analyzed for integrons. Only class 1 sul1 integrons containing aadA1a (28 strains), aadA1a-dfr1-sat1 (2 strains), and dfr1-aadA1a (1 strain) gene cassettes were found. The first two types were found in clinical isolates belong...

journal_title：Antimicrobial agents and chemotherapy

authors： Soto SM,Lobato MJ,Mendoza MC

更新日期：2003-01-01 00:00:00

abstract：:The in vitro activity of WY-49605 (SUN5555) (WY) was compared with those of cefaclor, cefixime, and amoxicillin-clavulanic acid against 2,958 consecutive clinical isolates from five medical centers and 402 respiratory pathogens from 18 other facilities. Most members of the family Enterobacteriaceae were inhibited by W...

journal_title：Antimicrobial agents and chemotherapy

authors： Sewell D,Barry A,Allen S,Fuchs P,McLaughlin J,Pfaller M

更新日期：1995-07-01 00:00:00

abstract：:A survey conducted between 1987 and 1994 at the University Hospital of Besançon, France, demonstrated a dramatic increase (from 0 to 42. 5%) in the prevalence of amoxicillin resistance among Salmonella spp. Of the 96 resistant isolates collected during this period (including 77 Typhimurium), 54 were found to produce T...

journal_title：Antimicrobial agents and chemotherapy

authors： Llanes C,Kirchgesner V,Plesiat P

更新日期：1999-10-01 00:00:00

abstract：:Granulocytopenia is a complication of human immunodeficiency virus disease, as well as a toxic manifestation of zidovudine therapy. To evaluate pharmacokinetic and pharmacodynamic relationships, 11 AIDS-AIDS-related complex patients who had developed zidovudine-associated granulocytopenia (mean absolute neutrophil cou...

journal_title：Antimicrobial agents and chemotherapy

authors： Hewitt RG,Morse GD,Lawrence WD,Maliszewski ML,Santora J,Bartos L,Bonnem E,Poiesz B

更新日期：1993-03-01 00:00:00

abstract：:We examined the in vitro susceptibilities of three reference strains and 41 recent clinical isolates of herpes simplex virus types 1 and 2 to 5-ethyl-2'-deoxyuridine. This thymidine analog exerts a type 2-preferential but not a type 2-specific antiviral effect. Utilizing a microtiter assay with BHK-21 cells, we found ...

journal_title：Antimicrobial agents and chemotherapy

authors： Teh CZ,Sacks SL

更新日期：1983-05-01 00:00:00

abstract：:Topical administration of live commensal bacteria to the vaginal tract holds significant potential as a cost-effective strategy for the treatment of sexually transmitted infections and the delivery of mucosal vaccines. Probiotic-releasing intravaginal rings (IVRs) embody significant theoretical advantages over traditi...

journal_title：Antimicrobial agents and chemotherapy

authors： Gunawardana M,Mullen M,Yoo J,Webster P,Moss JA,Baum MM

更新日期：2014-01-01 00:00:00

abstract：:Two morphotypes of Hafnia alvei differed in their susceptibilities to beta-lactam antibiotics. Both produced an inducible Bush group 1 beta-lactamase. Hyperinducibility of this enzyme was associated with reduced susceptibility in one morphotype. ...

journal_title：Antimicrobial agents and chemotherapy

authors： Thomson KS,Sanders CC,Washington JA 2nd

更新日期：1993-06-01 00:00:00

abstract：:The behavioral and convulsant effects of cefazolin, a beta-lactam derivative, were studied after intraperitoneal administration to DBA/2 mice, a strain genetically susceptible to sound-induced seizures, and Swiss mice. DBA/2 mice were more susceptible to seizures induced by cefazolin than were Swiss mice. The proconvu...

journal_title：Antimicrobial agents and chemotherapy

authors： De Sarro A,Zappalá M,Chimirri A,Grasso S,De Sarro GB

更新日期：1993-07-01 00:00:00

abstract：:Ciprofloxacin, clinafloxacin, PD 131628, sparfloxacin, and trovafloxacin were tested against 236 strains of Streptococcus pneumoniae, most of which were resistant to other agents. Resistance to multiple antibiotics did not affect the organism's susceptibility to the fluoroquinolones. The fluoroquinolones with in vitro...

journal_title：Antimicrobial agents and chemotherapy

authors： Barry AL,Fuchs PC,Brown SD

更新日期：1996-10-01 00:00:00

abstract：:The anilinouracils (AUs) such as 6-(3-ethyl-4-methylanilino)uracil (EMAU) are a novel class of gram-positive, selective, bactericidal antibacterials which inhibit pol IIIC, the gram-positive-specific replicative DNA polymerase. We have linked various fluoroquinolones (FQs) to the N-3 position of EMAU to generate a var...

journal_title：Antimicrobial agents and chemotherapy

authors： Butler MM,Lamarr WA,Foster KA,Barnes MH,Skow DJ,Lyden PT,Kustigian LM,Zhi C,Brown NC,Wright GE,Bowlin TL

更新日期：2007-01-01 00:00:00

abstract：:Olorofim is a novel antifungal drug in phase 2 trials. It has shown promising in vitro activity against various molds, except for Mucorales. Initially, we observed a broad range of EUCAST MICs for Aspergillus fumigatus Here, we explored the MIC variability in more detail and prospectively investigated the susceptibili...

journal_title：Antimicrobial agents and chemotherapy

authors： Astvad KMT,Jørgensen KM,Hare RK,Datcu R,Arendrup MC

更新日期：2020-12-16 00:00:00

abstract：:Using an immunocompetent murine model of invasive aspergillosis (IA), we previously reported that the efficacy of liposomal amphotericin B (L-AmB) (Ambisome) is not hampered by the presence of azole resistance mutations in Aspergillus fumigatus (S. Seyedmousavi, W. J. G. Melchers, J. W. Mouton, and P. E. Verweij, Anti...

journal_title：Antimicrobial agents and chemotherapy

authors： Seyedmousavi S,Mouton JW,Melchers WJG,Verweij PE

更新日期：2017-05-24 00:00:00

abstract：:A new class of pyridine oxide derivatives as inhibitors of human immunodeficiency virus type 1 (HIV-1) and/or HIV-2 replication in cell culture has been identified. The compounds, which specifically inhibit HIV-1, behave as typical nonnucleoside reverse transcriptase inhibitors (NNRTIs). The most active congener of th...

journal_title：Antimicrobial agents and chemotherapy

authors： Stevens M,Pannecouque C,De Clercq E,Balzarini J

更新日期：2003-09-01 00:00:00