Abstract：
:In Madin-Darby canine kidney (MDCK) cells, the effect of 2-O-methyl PAF, an inactive analogue of platelet activating factor (PAF), on intracellular Ca2+ concentration ([Ca2+]i) was measured by using the Ca2+-sensitive fluorescent dye fura-2. 2-O-methyl PAF (> or = 15 microM) caused a rapid rise of [Ca2+]i in a concentration-dependent manner. 2-O-methyl PAF-induced [Ca2+]i rise was partly reduced by removal of extracellular Ca2+. 2-O-methyl PAF-induced extracellular Ca2+ influx was also suggested by Mn2+ influx-induced fura-2 fluorescence quench. The 2-O-methyl PAF-induced Ca2+ influx was blocked by nifedipine, verapamil and diltiazem. In Ca2+-free medium, thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+-ATPase, caused a monophasic [Ca2+]i rise, after which 2-O-methyl PAF failed to increase [Ca2+]i; also, pretreatment with 2-O-methyl PAF depleted thapsigargin-sensitive Ca2+ stores. U73122, an inhibitor of phospholipase C, abolished ATP (but not 2-O-methyl PAF)-induced [Ca2+]i rise. These findings suggest that 2-O-methyl PAF evokes a rapid increase in [Ca2+]i in renal tubular cells by stimulating both extracellular Ca2+ influx and intracellular Ca2+ release.
journal_name
Life Scijournal_title
Life sciencesauthors
Yeh JH,Huang CJ,Lee JH,Hsu SS,Chen JS,Cheng HH,Chang HT,Huang JK,Chung HM,Mei-Yin Y,Jan CRdoi
10.1016/j.lfs.2004.10.064subject
Has Abstractpub_date
2005-06-03 00:00:00pages
336-44issue
3eissn
0024-3205issn
1879-0631pii
S0024-3205(05)00152-9journal_volume
77pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The somatostatin antagonist analogue cyclo Ahep-Phe-D-Trp-Lys-Thr(Bzl) stimulates growth in young female rats, while somatostatin itself has only equivocal effects on inhibiting growth. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90621-6
更新日期：1985-07-08 00:00:00
abstract：:Short-chain fatty acids (SCFA) are produced by fermentation of water-soluble fiber by anaerobic bacteria in the large bowel. Fiber-rich diets decrease the risk of developing inflammatory bowel disease (IBD) and butyrate enemas are effective as a therapy in some patients. Crohn's disease, one form of IBD, appears to in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00490-9
更新日期：2003-08-15 00:00:00
abstract：:In some cell systems muscarinic receptor stimulation can induce proliferation or transformation. This phenomenon is subtype-specific (only m1 and m3 receptors are effective) and cell type dependent. In 1321N1 astrocytoma cells activation of m3 receptors stimulates phospholipase C, but does not induce DNA synthesis. In...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:Despite the considerable number of published studies in the field of S-nitrosothiols (RSNO), the determination of these compounds in biological samples still represents an analytical challenge, due to several technical obstacles and often long sample preparation procedures. Other problems derive from the intrinsic lab...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2010.10.024
更新日期：2011-01-17 00:00:00
abstract：:The present study examined the discriminative stimulus and response rate-decreasing effects of kappa opioids in pigeons trained to discriminate a 0.017 mg/kg dose of bremazocine from saline. Bremazocine, spiradoline, CI977, U69,593 and U50,488 substituted completely for the bremazocine stimulus in a dose-dependent and...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90115-5
更新日期：1994-01-01 00:00:00
abstract：:Hydroxyethyl starch (HES) found in rat serum and spleen after single and daily administrations of 0.9 g/kg for 1 week was characterized by gas-liquid chromatography. There was very little difference in the degree of substitution (D.S.) and molar substitution (M.S.) of HES in serum samples obtained at 1 hour and 57 day...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90737-3
更新日期：1983-11-14 00:00:00
abstract：:Hepatic tryptophan 2,3-dioxygenase (TDO) is one of the rate-limiting enzymes in tryptophan catabolism and plays an important role in regulating the physiological flux of tryptophan into relevant metabolic pathways. In this study, we determined the effect of the non-steroidal anti-inflammatory agents, tolmetin and suli...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.028
更新日期：2006-11-10 00:00:00
abstract：:Alpha-lipoic acid (ALA), a potent biological antioxidant, improves insulin action of skeletal muscle glucose transport and metabolism in both human and animal models of insulin resistance. In order to obtain further insight into the potential intracellular mechanisms for the action of ALA on insulin-stimulated glucose...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00562-6
更新日期：1997-01-01 00:00:00
abstract：:The association between various parameters of acute and chronic smoking status and plasma levels of three proteins, C9, C1-inhibitor (C1-INH) and alpha 1-protease inhibitor (alpha 1-PI) were determined for 49 male cigarette smokers and 49 age-matched nonsmokers (mean age = 32.2 years). The mean number of cigarettes sm...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90391-1
更新日期：1985-03-11 00:00:00
abstract：AIMS:This study aims to evaluate the relationship between the amount of physical activity and different traditional and novel cardiometabolic risk factors, as well as atheroprotective agents, in male children and adolescents. MAIN METHODS:Cross-sectional study. A total of 337 male children and adolescents aged 7-14 ye...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.05.021
更新日期：2013-07-30 00:00:00
abstract：AIM:MicroRNAs (miRNAs) function as oncogenes or tumor suppressors in an extensive variety of human cancers, including prostate cancer (PCa). Herein, the aim of this study is to investigate the effect of miR-381 on autophagy and apoptosis of PCa cells through the reelin (RELN)-mediated the PI3K/AKT/mTOR signaling pathwa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117672
更新日期：2020-08-01 00:00:00
abstract：:To determine the mechanism of anoxic vasoconstriction, precontracted rat aortic rings were exposed to 95% N2, which caused additional contraction. Reoxygenation (95% O2) resulted in initial relaxation followed by contraction. Indomethacin did not affect anoxic contraction or reoxygenation-mediated events, but NG-monom...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90316-4
更新日期：1991-01-01 00:00:00
abstract：:Despite the recent scientific advances made in cancer diagnostics and therapeutics, cancer still remains the second leading cause of death worldwide. Thus, there is a need to identify new potential biomarkers/molecular targets to improve the diagnosis and treatment of cancer patients. In this regard, long non-coding R...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118035
更新日期：2020-09-15 00:00:00
abstract：:The distribution of immunoreactive-dynorphin (ir-Dyn) in isolated subpopulations of bovine adrenal chromaffin cells was examined and compared with that of adrenaline (A), noradrenaline (NA) and ir-Leucine-Enkephalin (ir-Leu-Enk). Using a stepwise bovine serum albumin (BSA) gradient, various populations of catecholamin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90042-5
更新日期：1983-01-17 00:00:00
abstract：:Platelet functions are becoming the useful tool for delineating the etiology of pregnancy induced hypertension. Electronmicroscopic studies, efflux and content of 5-HT in platelets and platelet aggregation responses towards various aggregating agents have been measured in 25 normotensive pregnant subjects and 31 PIH s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00735-7
更新日期：1994-01-01 00:00:00
abstract：:The muscarinic pharmacology of C1-methyl-substituted chiral compounds related to McN-A-343 and of (R)- and (S)-dimethindene has been studied. Among the McN-A-343 analogues, the (S)-enantiomers were more potent and had higher affinity than the (R)-isomers. The quaternary compound (S)-BN 228 was found to be the most pot...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00015-x
更新日期：1995-01-01 00:00:00
abstract：AIMS:To investigate the effects of aerobic exercise training on cardiomyocyte ultrastructure, oxidative stress, and activation of protein synthesis pathways in a model of cardiomyopathy induced by doxorubicin (Dox). MAIN METHODS:Male Sprague Dawley rats were randomly assigned to Control (saline, sedentary), Dox/sedent...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118698
更新日期：2021-01-01 00:00:00
abstract：AIMS:This study aims to develop and evaluate oleuropein loaded surface functionalized folate-targeted - PEG liposomes for the effective management of prostate cancer in an animal model. MATERIALS AND METHODS:Film hydration-cum-extrusion technique was used to produce liposomes. Particle size, entrapment efficiency, dru...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.01.053
更新日期：2019-03-01 00:00:00
abstract：:The hypoglycemic and hypolipidemic effects of Lycium barbarum fruit water decoction, crude polysaccharide extracts (crude LBP), and purified polysaccharide fractions (LBP-X) in alloxan-induced diabetic or hyperlipidemic rabbits were investigated through designed sequential trials and by measuring blood glucose and ser...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.04.056
更新日期：2004-11-26 00:00:00
abstract：:14C-Imipramine was administered to male and female Sprague-Dawley rats and animals were sacrificed at 4, 8, 12, and 20 hours later. At all time points, total radioactivity in female lung was several-fold higher than in males. In addition, female lungs had a higher concentration of desmethylimipramine (DMI) as compared...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90610-5
更新日期：1982-03-01 00:00:00
abstract：AIMS:T lymphocytes synthesize acetylcholine (ACh) and express muscarinic and nicotinic ACh receptors (mAChR and nAChR, respectively) responsible for increases in the intracellular Ca2+ concentration ([Ca2+]i). Our aim in the present study was to assess whether autocrine ACh released from T lymphocytes regulates their p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.12.015
更新日期：2017-03-01 00:00:00
abstract：:SARS-CoV-2 infection or COVID-19 has become a worldwide pandemic; however, effective treatment for COVID-19 remains to be established. Along with acute respiratory distress syndrome (ARDS), new and old cardiovascular injuries are important causes of significant morbidity and mortality in COVID-19. Exploring new approa...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2021.119130
更新日期：2021-01-26 00:00:00
abstract：:Aspartate (ASP), glutamate (GLU), noradrenaline (NA), dopamine (DA) and its acidic metabolites DOPAC and HVA, serotonin (5-HT) and its metabolite 5-HIAA were simultaneously investigated in post-mortem tissue samples from right parahippocampal gyrus (temporal cortex) and frontal cortical pole (frontal cortex) of adults...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00067-2
更新日期：1997-01-01 00:00:00
abstract：AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.08.015
更新日期：2012-10-05 00:00:00
abstract：:New types of alpha1-adrenoceptor antagonists (tamsulosin, KMD-3213 and JTH-601) are currently receiving a great deal of attention, especially in terms of developing effective therapeutic agents to treat bladder outlet obstruction with less side effects, such as postural hypotension, in patients with benign prostatic h...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00111-8
更新日期：1998-01-01 00:00:00
abstract：:A recent neuropathological study has reported decreased levels of dynorphin A immunoreactivity in striato-pallidal fibers in the brain of a patient with severe Gilles de la Tourette's syndrome (TS). This observation, taken with the neuroanatomic distribution of dynorphin and its broad range of motor and behavioral eff...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90575-9
更新日期：1988-01-01 00:00:00
abstract：:In an in vitro bioassay system for adrenocorticotropic hormone using isolated rat adrenal cells, kaurenol, a diterpene alcohol, stimulated corticosterone production and augmented the steroidogenic effect of adrenocorticotropin or forskolin, dose-dependently. Kaurenol had no effect on cyclic AMP production by the cells...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90070-6
更新日期：1986-02-03 00:00:00
abstract：:The loss of osteoblast function in aging bone is one of the major causes of osteopenia, or loss of bone mass. In this study, this loss of function was investigated by examining the proliferative response of rat long bone periosteal osteoblasts to TGF(beta1) and 1,25-dihydroxy vitamin D(3) (1,25-D(3)) as a function of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01548-5
更新日期：2002-05-10 00:00:00
abstract：:Ovine fetal renal responses to ANF decrease during the last third of gestation, although circulating fetal plasma atrial natriuretic factor (ANF) levels are higher than in the maternal circulation, and do not change with gestation. This study examined whether previously reported maturational changes in fetal renal res...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00420-x
更新日期：1994-01-01 00:00:00
abstract：AIMS:Pulmonary hypertension (PH) is a proliferative disorder characterized by enhanced proliferation and suppressed apoptosis of intrapulmonary vascular smooth muscle cells. Recently, network-based bioinformatics have identified let-7 family, a tumor suppressive microRNA, regulate multiple interacting targets relevant ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.11.020
更新日期：2017-02-01 00:00:00
