Responses of GPCR135 to human gene 3 (H3) relaxin in CHO-K1 cells determined by microphysiometry.

Abstract:

:This study examined the functional response to human relaxin 2 (H2 relaxin), human relaxin 3 (H3 relaxin), porcine relaxin, and human INSL3 in the cytosensor microphysiometer, using CHO-K1 cells stably expressing human GPCR135. CHO-K1 cells stably expressing GPCR135 were generated by the serial dilution method and receptor properties were assessed. Saturation studies of [125I] H3 relaxin binding to GPCR135 in these cells gave a Bmax of 32.61 +/- 6.5 fmol/mg protein and Kd of 0.12 +/- 0.08 nM. The functional response to H3 relaxin and other relaxin/insulin peptides of GPCR135 expressed in CHO-K1 cells was measured in the cytosensor microphysiometer and analyzed using inhibitors of signal transduction proteins.

journal_name

Ann N Y Acad Sci

authors

Van der Westhuizen ET,Sexton PM,Bathgate RA,Summers RJ

doi

10.1196/annals.1282.053

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

332-7

eissn

0077-8923

issn

1749-6632

pii

1041/1/332

journal_volume

1041

pub_type

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