Abstract:
:Liolaemus lizards were explored to ascertain whether they would make an amenable model to study single-cell electrophysiology of neurons in the vomeronasal organ (VNO). Despite a rich array of chemosensory-related behaviors chronicled for this genus, no anatomical or functional data exist for the VNO, the organ mediating these types of behaviors. Two Liolaemus species (L. bellii and L. nigroviridis) were collected in Central Chile in the Farellones Mountains and transported to the United States. Lizards were subjected to hypothermia and then a lethal injection of sodium pentabarbitol prior to all experiments described in the following text. Retrograde dye perfusion combined with histological techniques demonstrated a compartmentalization of the proportionally large VNO from the main olfactory epithelium (MOE) in cryosections of L. bellii. SDS-PAGE analysis of the VNO of both species demonstrated the expression of three G protein subunits, namely, G(alphao), G(alphai2), and G(beta), and the absence of G(alphaolf), G(alpha11), and G(q), the latter of which are traditionally found in the MOE. Vomeronasal (VN) neurons were enzymatically isolated for whole cell voltage-clamp electrophysiology of single neurons. Both species demonstrated a tetrodotoxin (TTX)-sensitive, rapidly inactivating sodium current and a tetraethylammonium (TEA)-sensitive potassium current that had a transient and sustained component. VN neurons were classified into two types dependent on the ratio of sodium over sustained potassium current. VN neurons exhibited outward and inward chemosignal-evoked currents when stimulated with pheromone-containing secretions taken from the feces, skin, and precloacal pores. Fifty-nine percent of the neurons were responsive to at least one compound when presented with a battery of five different secretions. The breadth of responsiveness (H metric) demonstrated a heterogeneous population of tuning with a mean of 0.29.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Labra A,Brann JH,Fadool DAdoi
10.1152/jn.00490.2005subject
Has Abstractpub_date
2005-10-01 00:00:00pages
2535-48issue
4eissn
0022-3077issn
1522-1598pii
00490.2005journal_volume
94pub_type
杂志文章abstract::1. Effects of inositol-1,4,5-trisphosphate (InsP3) applied through a patch pipette to Xenopus laevis olfactory receptor cells (ORCs) were studied using the patch-clamp technique in conjunction with calcium imaging with fura-2. 2. InsP3 activated, first, a novel voltage-independent Ca2+ current (ICa) and, second, a non...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.73.2.862
更新日期:1995-02-01 00:00:00
abstract::Divergence of the Shaker superfamily of voltage-gated (Kv) ion channels early in metazoan evolution created numerous electrical phenotypes that were presumably selected to produce a wide range of excitability characteristics in neurons, myocytes, and other cells. A comparative approach that emphasizes this early radia...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00858.2005
更新日期:2006-05-01 00:00:00
abstract::Few studies have analyzed activity-induced changes in EMG activity in individual human motor units. We studied the changes in human thenar motor unit EMG that accompany the potentiation of twitch force and fatigue of tetanic force. Single motor unit EMG and force were recorded in healthy subjects in response to select...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章
doi:10.1152/jn.00924.2005
更新日期:2006-03-01 00:00:00
abstract::Orexin-producing neurons are clearly essential for the regulation of wakefulness and sleep because loss of these cells produces narcolepsy. However, little is understood about how these neurons dynamically interact with other wake- and sleep-regulatory nuclei to control behavioral states. Using survival analysis of wa...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01243.2007
更新日期:2008-06-01 00:00:00
abstract::We used positron emission tomography to study motor learning by trial and error. Subjects learned sequences of eight finger movements. Tones generated by a computer told the subjects whether any particular move was correct or incorrect. A control condition was used in which the subjects generated moves, but there was ...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1152/jn.1997.77.3.1325
更新日期:1997-03-01 00:00:00
abstract::The functional and biophysical properties of a persistent sodium current (I(NaP)) previously proposed to participate in the generation of subthreshold oscillations and burst discharge in mesencephalic trigeminal sensory neurons (Mes V) were investigated in brain stem slices (rats, p7-p12) using whole cell patch-clamp ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00636.2004
更新日期:2005-05-01 00:00:00
abstract::Intracellular recordings were made from rhythm-generating interneurons in the Lymnaea feeding system. The feeding pattern is a three-phase rhythm of interneuronal activity (N1, N2, N3) corresponding to protraction, rasp, and swallow. We describe the firing pattern and anatomy of the premotor interneurons, each of whic...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1985.54.6.1396
更新日期:1985-12-01 00:00:00
abstract::Feedback control of our limbs must account for the unexpected offset of mechanical perturbations. Here we examine the evoked activity of elbow flexor and extensor muscles to torque pulses lasting 22-152 ms and how torque offset impacts activity in the long-latency (45-100 ms) and voluntary epochs (120-180 ms). For eac...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01139.2009
更新日期:2010-06-01 00:00:00
abstract::Intense itch and urge to scratch are the major symptoms of many chronic skin ailments, which are increasingly common. Vicious itch-scratch cycles are readily established and may diminish quality of life for those afflicted. We investigated peripheral and central processing of two types of itch sensation elicited by sk...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00070.2006
更新日期:2007-01-01 00:00:00
abstract::1. The sensorimotor synapse of Aplysia expresses various shortlasting changes in synaptic efficacy including homosynaptic depression (HSD) and heterosynaptic facilitation by serotonin (5-HT) either at nondepressed sensory neuron (SN) synaptic connections or at SN synaptic connections first depressed by HSD. We examine...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.4.2250
更新日期:1996-10-01 00:00:00
abstract::Neurons display a variety of complex dendritic morphologies even within the same class. We examined the relationship between dendritic arborization and the coupling between somatic and dendritic action potential (AP) initiation sites in layer 5 (L5) neocortical pyramidal neurons. Coupling was defined as the relative r...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00046.2003
更新日期:2003-06-01 00:00:00
abstract::The turtle posterior crista consists of two hemicristae. Each hemicrista extends from the planum semilunatum to the nonsensory torus and includes a central zone (CZ) surrounded by a peripheral zone (PZ). Type I and type II hair cells are found in the CZ and are innervated by calyx, dimorphic and bouton afferents. Only...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.3.1202
更新日期:2000-03-01 00:00:00
abstract::1. The aim of this study was to characterize the signals transmitted by neurons of the medial vestibular nucleus (MVN) to the middle zone of the flocculus in alert cats. 2. Bipolar stimulating electrodes were implanted into the middle zone of each flocculus, because this zone is known to be involved in the control of ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.3.1759
更新日期:1996-09-01 00:00:00
abstract::Previous studies have shown that daily, moderate-level sound exposure, or conditioning, can reduce injury from a subsequent high-level noise exposure. We tested the hypothesis that this conditioning produces an increased activity in the olivocochlear efferent reflex, a reflex known to provide protection to the cochlea...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.6.3088
更新日期:1998-06-01 00:00:00
abstract::The rostral ventromedial medulla (RVM) modulates nociception through changes in the activity of two classes of neuron, ON- and OFF-cells. The activity of these neurons is regulated, in part, by input from the periaqueductal gray (PAG). The objective of this study was to determine whether PAG-mediated antinociception i...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.88.4.1707
更新日期:2002-10-01 00:00:00
abstract::Whenever the eyes move, spatial attention must keep track of the locations of targets as they shift on the retina. This study investigated transsaccadic updating of visual attention to cued targets. While observers prepared a saccade, we flashed an irrelevant, but salient, color cue in their visual periphery and measu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00656.2012
更新日期:2013-03-01 00:00:00
abstract::1. Microelectrodes filled with horseradish peroxidase (HRP) were inserted in the superior colliculus (SC) of alert squirrel monkeys. Spontaneous eye movements were monitored in the dark during recording and intraaxonal injection of fibers carrying presaccadic signals. 2. Analysis of the relationship between neuronal a...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.60.1.263
更新日期:1988-07-01 00:00:00
abstract::Slowness of voluntary movements in patients with multiple sclerosis (MS) may be due to various factors, including attentional and cognitive deficits, delays in motor conduction time, and impairment of specific central nervous system circuits. In 13 healthy volunteers and 20 mildly disabled, relapsing-remitting MS pati...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00591.2014
更新日期:2015-03-01 00:00:00
abstract::Identifying the properties of correlations in the firing of neocortical neurons is central to our understanding of cortical information processing. It has been generally assumed, by virtue of the columnar organization of the neocortex, that the firing of neurons residing in a certain vertical domain is highly correlat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00981.2014
更新日期:2015-05-01 00:00:00
abstract::Precisely synchronized neuronal activity has been commonly observed in the mammalian visual pathway. Spike timing correlations in the lateral geniculate nucleus (LGN) often take the form of phase synchronized oscillations in the high gamma frequency range. To study the relations between oscillatory activity, synchrony...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01000.2009
更新日期:2010-12-01 00:00:00
abstract::Limb movement-related neurons in the cerebellar nuclei (CN) typically produce bursts of discharge in association with movement. Consequently, given the inhibitory nature of the Purkinje cell (PC) projection to CN, it is puzzling that only a minority of movement-related PCs pause; the majority burst. Some of the moveme...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00603.2004
更新日期:2005-01-01 00:00:00
abstract::The current eye-tracking study examined the influence of reward on oculomotor performance, and the extent to which learned stimulus-reward associations interacted with voluntary oculomotor control with a modified paradigm based on the classical antisaccade task. Participants were shown two equally salient stimuli simu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00275.2018
更新日期:2018-10-01 00:00:00
abstract::The movement representation in the primary motor cortex (M1) of the cat develops between postnatal weeks 7-12. The somatosensory representation in motor cortex is present by the age that the motor map begins to develop. In this study we examined the role of neural activity in development and maintenance of the M1 move...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00424.2005
更新日期:2005-11-01 00:00:00
abstract::Basal ganglia circuits are organized as parallel loops that have been proposed to compete in a winner-take-all fashion to determine the appropriate behavioral outcome. However, limited experimental support for strong lateral inhibition mechanisms within striatal regions questions this model. Here, stimulation of the p...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91002.2008
更新日期:2009-04-01 00:00:00
abstract::We have previously reported that short-term (48-72 h) exposure to the GABA-modulatory steroid 3alpha-OH-5alpha-pregnan-20-one (3alpha,5alpha-THP) increases expression of the alpha4 subunit of the GABA(A) receptor (GABAR) in the hippocampus of adult rats. This change in subunit composition was accompanied by altered ph...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00780.2002
更新日期:2003-04-01 00:00:00
abstract::1. Extracellular spikes of burster-driving neurons (BDNs) were recorded within and immediately below the prepositus hypoglossi nucleus in the alert cat. BDNs were characterized by short-latency activation after stimulation of the contralateral vestibular nerve (latency: 1.4-2.7 ms) and the ipsilateral superior collicu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.1.273
更新日期:1995-07-01 00:00:00
abstract::A powerful early inhibition is seen in triceps surae after transcutaneous electrical stimulation of the Achilles tendon [tendon electrical stimulation (TES)]. The aim of the present study was to confirm results from surface electromyogram (SEMG) recordings that the inhibition is not wholly or partly due to stimulation...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00751.2011
更新日期:2012-01-01 00:00:00
abstract::1. The responses of a total of 70 single neurons were recorded from the parabrachial nuclei (PBN) in awake rats. In 59 neurons, sapid stimuli (0.5 ml) elicited significant taste responses. Of these 59 neurons, 10 also had significant responses to water. The mean spontaneous rate of the taste neurons was 13.4 +/- 6.9 (...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.63.4.707
更新日期:1990-04-01 00:00:00
abstract::We studied the effects of fatigue of the index finger on indices of force variability in discrete and rhythmic accurate force production tasks performed by the index finger and by all four fingers pressing in parallel. An increase in the variance of the force produced by the fatigued index finger was expected. We hypo...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00077.2010
更新日期:2010-06-01 00:00:00
abstract::1. Many examples of long-term potentiation (LPT) are induced by repetitive electrical stimulation of presynaptic axons. LTP also is induced by direct glutamate iontophoresis (1 M, 1-2 microA, 10 s) onto postsynaptic neurons in hippocampal slices without evoked presynaptic stimulation; this form of LTP is called "ionto...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.75.5.1909
更新日期:1996-05-01 00:00:00