当前位置： SCI文献检索 > Orthodontics & Craniofacial Research期刊下所有文献 > Non-viral-mediated gene therapy approaches for bone repair.

Non-viral-mediated gene therapy approaches for bone repair.

Abstract：

OBJECTIVES:Bone repair strategies continue to be developed for alternatives to autografting, allogeneic implants of banked bone, and other bone substitutes. Efforts have included the delivery of potent growth and/or differentiation factors and the use of gene therapy. For bone regeneration, gene therapy is the delivery, uptake and expression of DNA that has been localized to a wound bed. The objective of the current study is to investigate methods to enhance non-viral-mediated means of gene uptake and expression for use in bone regeneration. METHODS:Several types of DNA-polymer complexes, either applied directly to baby hamster kidney (BHK) cells, or released from a porous, resorbable gene-activated matrix (GAM), were evaluated in vitro for their ability to transfect cells with a circular plasmid DNA construct expressing green fluorescent protein. Complexes included conjugates containing a lipophilic reagent, liposomes, poly-ethyl-oxazoline, and poly-ethyleneimine (PEI). Data were subjected to analysis of variance and Fisher's protected least significant difference for multiple comparisons with significance established at p < 0.05. RESULTS:Transfection efficiencies of the liposome and PEI complexes improved in vitro when released from resorbable GAMs. The lipophilic reagent FuGene 6 demonstrated abundant uptake and expression in the initial 1- and 2-day evaluation periods. In contrast, the DNA-liposome and PEI GAM complexes demonstrated a sustained release, uptake and expression by the BHK cells at the 2-, 4-, and 7-day, and 4- and 7-day evaluation intervals, respectively. CONCLUSION:GAM technology appears to improve the functional stability and release duration of incorporated DNA-polymer complexes in the present in vitro studies. The ongoing objective of our research is to develop a localized treatment to improve the uptake and expression of plasmid DNA by non-viral-mediated gene therapy.

journal_name

Orthod Craniofac Res

journal_title

Orthodontics & craniofacial research

authors

Winn SR,Chen JC,Gong X,Bartholomew SV,Shreenivas S,Ozaki W

doi

10.1111/j.1601-6343.2005.00332.x

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

183-90

issue

eissn

1601-6335

issn

1601-6343

pii

OCR332

journal_volume

pub_type

杂志文章

在线工具

Non-viral-mediated gene therapy approaches for bone repair.