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Electrophoresis of lymphoid cells. Differences in the cell membrane structure of murine thymocytes, T and B cells revealed by enzyme and formalin treatment.

Abstract：

:Low concentrations of protease and trypsin reduced the electrophoretic mobility (EPM) of thymocytes; with higher concentrations it was normal or above. Differences in membrane structure of thymocytes, T and B cells was found as B cells showed no reduction while T cells gave intermediate values. Further the reduction was greater with protease than with trypsin. Formalin fixation increased the EPM of all normal cell types to a similar degree. The EPM of proteolytically treated thymocytes and B cells was increased to a similar level and to a greater degree than neuraminidase-treated thymocytes. Small amounts of sialic acid were detected in the supernatant after proteolytic treatment of thymocytes. Protease reduced the binding of anti-lymphocyte serum, while no definite effect was obtained with trypsin. Neither sublytic doses of phospholipase C nor ribonuclease appeared to change the EPM.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Wiig JN

subject

Has Abstract

pub_date

1975-10-01 00:00:00

pages

91-101

issue

1

eissn

0009-9104

issn

1365-2249

journal_volume

22

pub_type

杂志文章

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