Abstract:
:The tumour necrosis factor (TNF)-α-induced proteins (TNFAIP)9 and TNFAIP3 play an important pathogenic role in murine arthritis. To clarify their pathophysiological roles in patients with rheumatoid arthritis (RA), we examined their expression and localization in peripheral blood mononuclear cells (PBMC). TNFAIP9 and TNFAIP3 mRNA expression was determined in PBMC of RA patients and healthy subjects (control). Flow cytometry was used to analyse the main TNFAIP9- and TNFAIP3-expressing cell populations. TNFAIP9 and TNFAIP3 mRNA expression levels were examined in vitro on CD14(+) cells stimulated with TNF-α and lipopolysaccharide (LPS). The expression levels of TNFAIP9 and TNFAIP3 mRNA were also measured before and 12 weeks after treatment with tocilizumab and abatacept. TNFAIP9 expression was significantly higher, while TNFAIP3 expression was lower in PBMC of RA (n=36) than the control (n=24) (each P < 0.05). TNFAIP9 was expressed on CD14(+) cells, especially in human leucocyte antigen D-related (HLA-DR)(+) CD14(bright) CD16(-) cells, while TNFAIP3 was expressed mainly on CD3(+) T cells. TNF-α and LPS induced TNFAIP9 and TNFAIP3 in human CD14(+) monocytes in vitro. Treatment with tocilizumab (n=13), but not abatacept (n=11), significantly reduced TNFAIP9 mRNA expression in PBMC, which was associated with reduction in the number of circulating CD14(bright) monocytes. The expression of TNFAIP9 in CD14(+) cells was specifically elevated in patients with RA, regulated by TNF-α and LPS, and suppressed by tocilizumab, while TNFAIP3 in PBMC showed different localization and induction patterns.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Takai C,Matsumoto I,Inoue A,Umeda N,Tanaka Y,Kurashima Y,Wada Y,Narita I,Sumida Tdoi
10.1111/cei.12606subject
Has Abstractpub_date
2015-06-01 00:00:00pages
458-66issue
3eissn
0009-9104issn
1365-2249journal_volume
180pub_type
杂志文章abstract::One of the hallmarks of coeliac disease, both active and treated, is an increased number and proportion of gamma/delta intraepithelial T lymphocytes in the small intestinal mucosa, and an increased number of gamma/delta T cells in the small intestinal mucosa of coeliac disease patients has been associated with the inh...
journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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doi:
更新日期:1977-06-01 00:00:00
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journal_title:Clinical and experimental immunology
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doi:
更新日期:1983-02-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1996.d01-827.x
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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journal_title:Clinical and experimental immunology
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doi:
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journal_title:Clinical and experimental immunology
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doi:
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pub_type: 杂志文章,评审
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