Abstract:
:Lectins, known to recognize endothelial cell adhesion molecules, have been shown to release endothelium-derived relaxing factor (EDRF) from blood vessels. We investigated the effects of different leukocyte-type cells to determine if these cells, by interacting with the endothelium, could release EDRF from the circumflex branch of the canine coronary artery. The following cells were investigated: human promyelocytic leukemia (HL-60), human monocyte (THP-1), and human Burkitt lymphoma (DAUDI). All of these cells produced a significant endothelium-dependent relaxation of the dog coronary artery in the presence of ibuprofen. The endothelium-dependent relaxations were reversed by hemoglobin (10 microM), methylene blue (3 microM), 6-anilino-5,8-quinolinedione (LY 83583, 30 microM), and NG-nitro-L-arginine methyl ester (L-NAME, 1 mM). HL-60 cells grown in the presence of 1 mM L-NAME retained their ability to cause endothelium-dependent relaxation of the canine coronary artery, suggesting that the source of the NO was the endothelium and not the HL-60 cells. The cell-induced vascular relaxation could be obtained in the absence of extracellular calcium. It is suggested that HL-60, THP-1, and DAUDI cells interact with a specific receptor on the endothelial cell and as a result of this interaction the endothelial cells are stimulated to release EDRF.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Kleha JF,Devesly P,Johns Adoi
10.1139/y95-052subject
Has Abstractpub_date
1995-03-01 00:00:00pages
404-8issue
3eissn
0008-4212issn
1205-7541journal_volume
73pub_type
杂志文章abstract::The interactions of ligands with their receptors and the modulation of those interactions by other ligands are commonly described in the (verbal and mathematical) language of enzymology. While this analogy is appropriate in that velocity-substrate concentration curves and ligand-receptor binding isotherms often confor...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y88-059
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
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abstract::We investigated the influence of four different culture media: 20% fetal bovine serum (FBS), 5% FBS, 5% FBS supplemented with 10 mg x L(-1) linoleic acid (18:2(n-6)) or alpha-linolenic acid (18:3(n-3)) on alpha-linolenic acid apical uptake in clone TC7 of human intestinal Caco-2 cell line. Neither cellular viability n...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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abstract::Magnesium concentrations in erythrocyte ghosts and arterial tissue of male, spontaneously hypertensive rats (SHR) were significantly less than in these tissues of male normotensive controls (Wistar-Kyoto; WKY) of the same age, which were also fed rat chow and tap water. The magnesium concentration in SHR erythrocyte g...
journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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更新日期:1995-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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更新日期:1990-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
doi:
更新日期:1996-06-01 00:00:00
abstract::Recent studies demonstrate the critical role of the extracellular matrix in the organization of parenchymal cells in the heart. Thus, an understanding of the modes of regulation of matrix production by cardiac myofibroblasts is essential. Transforming growth factor beta (TGF-beta) signaling is transduced through the c...
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