Inhibition of angiotensin II- and endothelin-1-stimulated proliferation by selective MEK inhibitor in cultured rabbit gingival fibroblastsdagger.


:We investigated the implication of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) in the proliferation stimulated by angiotensin II (Ang II) and endothelin-1 (ET-1) in cultured rabbit gingival fibroblasts (CRGF). Ang II stimulated activation of ERK1/2 and the activation was inhibited by CV-11974, an AT1 antagonist, and saralasin, an AT1/AT2 antagonist, but not by PD123,319, an AT2 antagonist in the CRGF. Ang II-stimulated proliferation was inhibited by PD98059 or U0126, selective MEK inhibitors. Furthermore, ET-1 stimulated proliferation via G-protein-coupled ETA receptors, which were identified by Western blot analysis of membrane protein from the CRGF. ET-1 also stimulated activation of ERK1/2 and the activation was inhibited by BQ-123, an ETA inhibitor, and TAK044, an ETA/ETB inhibitor, but not by BQ-788, an ETB inhibitor. ET-1-stimulated proliferation was inhibited by PD98059 or U0126. These findings suggest that ERK1/2 play a role in the signaling process leading to proliferation stimulated by Ang II and ET-1 via G-protein-coupled receptors, AT1 and ETA in CRGF.


Fundam Clin Pharmacol


Ohsawa M,Ohuchi N,Taniguchi Y,Kizawa Y,Koike K,Iwamoto K,Hayashi K,Murakami H




Has Abstract


2005-12-01 00:00:00














  • Medicinal plants and the treatment of diabetes in Senegal: survey with patients.

    abstract::Diabetes is the most common metabolic disorder worldwide and is a major public health problem. Its frequency increases every day in all countries. However, in developing African countries, few people have access to drugs. In addition, in Africa, traditional beliefs induce people to use medicinal plants whenever they h...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Dièye AM,Sarr A,Diop SN,Ndiaye M,Sy GY,Diarra M,Rajraji Gaffary I,Ndiaye Sy A,Faye B

    更新日期:2008-04-01 00:00:00

  • Inhibitory effects of tacrine and physostigmine on catecholamine secretion and membrane currents in guinea-pig adrenal chromaffin cells.

    abstract::The effects of tacrine and physostigmine on catecholamine secretion induced by veratridine and high K+, and on voltage-dependent Na+ and Ca2+ currents, were investigated in guinea-pig adrenal chromaffin cells. In perfused adrenal glands, tacrine (100 microM) caused an inhibition of veratridine-induced catecholamine se...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Sugawara T,Kitamura N,Ohta T,Ito S,Nakazato Y

    更新日期:1998-01-01 00:00:00

  • Pharmacokinetic and pharmacodynamic study of suriclone imipramine interaction in man.

    abstract::Suriclone is a novel cyclopyrrolone exhibiting anxiolytic activity. Twelve healthy Caucasian male volunteers participated in the study. A single dose of suriclone 0.4 mg, imipramine 75 mg, suriclone 0.4 mg + imipramine 75 mg, or placebo was given according to a 4 x 4 Latin-square design in order to assess the effect o...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Perault MC,Chapelle G,Bouquet S,Chevalier P,Montay G,Gaillot J,Chakroun H,Guillet P,Vandel B

    更新日期:1994-01-01 00:00:00

  • Pharmacological evidence of involvement of nitric oxide pathway in anti-pruritic effects of sumatriptan in chloroquine-induced scratching in mice.

    abstract::Chloroquine (CQ) induces histamine-independent itch in human and mice. We recently reported the role of intradermal nitric oxide (NO)/cyclic guanosine monophosphate pathway in CQ-evoked scratching in mice. Chloroquine stimulates neuronal nitric oxide synthase (nNOS) activity to over-producing NO in the skin. Sumatript...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Haddadi NS,Ostadhadi S,Shakiba S,Afshari K,Rahimi N,Foroutan A,Dehpour AR

    更新日期:2018-02-01 00:00:00

  • Blockade of angiogenin by thalidomide inhibits the tumorigenesis of murine hemangioendothelioma.

    abstract::Thalidomide, a well-known immunomodulatory compound, has an anti-angiogenic activity, which may be utilized for the treatment of angiogenesis-related diseases such as hemangioendothelioma. The aim of the present study was to investigate both the antitumor role of thalidomide on hemangioendothelioma and the underlying ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Guo L,Wan Z,Xu B,Ren L,Liu H,Song N,Liu L

    更新日期:2019-12-01 00:00:00

  • Zinc: indications in brain disorders.

    abstract::Zinc is the authoritative metal which is present in our body, and reactive zinc metal is crucial for neuronal signaling and is largely distributed within presynaptic vesicles. Zinc also plays an important role in synaptic function. At cellular level, zinc is a modulator of synaptic activity and neuronal plasticity in ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Prakash A,Bharti K,Majeed AB

    更新日期:2015-04-01 00:00:00

  • Calcium antagonists and thiazide diuretics have opposite effects on blood rheology and radial artery compliance in arterial hypertension: a randomized double-blind study.

    abstract::In addition to their effects on blood pressure, antihypertensive agents may produce additional effects on blood rheology and arterial compliance abnormalities which may play a role in target-organ damage. However, these effects may depend only on the specific pharmacological properties of certain antihypertensive agen...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Khder Y,Bray des Boscs L,el Ghawi R,Meilhac B,Montestruc F,Stoltz JF,Zannad F

    更新日期:1998-01-01 00:00:00

  • Evidence for the involvement of the serotonergic, noradrenergic, and dopaminergic systems in the antidepressant-like action of riparin III obtained from Aniba riparia (Nees) Mez (Lauraceae) in mice.

    abstract::Previous work has shown that intraperitoneal administration of riparin III (ripIII) reduces immobility time in the forced swimming test (FST), which suggests potential antidepressant activity. As the mechanism of action is not completely understood, this study is aimed at investigating the antidepressant-like action o...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Melo CT,de Carvalho AM,Moura BA,Teixeira CP,Vasconcelos LF,Feitosa ML,de Oliveira GV,Barbosa-Filho JM,Chavez Gutierrez SJ,de França Fonteles MM,Vasconcelos SM,de Sousa FC

    更新日期:2013-02-01 00:00:00

  • Functional, endogenously expressed corticotropin-releasing factor receptor type 1 (CRF1) and CRF1 receptor mRNA expression in human neuroblastoma SH-SY5Y cells.

    abstract::Corticotropin-releasing factor (CRF) is a hypothalamic 41-amino acid peptide which stimulates corticotropin (ACTH) release from the anterior pituitary and is also involved in the body response to stress. CRF1 receptors represent a potential target for novel antidepressant/anxiolytic drugs. The aim of the present study...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Schoeffter P,Feuerbach D,Bobirnac I,Gazi L,Longato R

    更新日期:1999-01-01 00:00:00

  • Effect of angiotensin converting enzyme inhibitors on quality of life in hypertensive patients. Pharmacodynamic basis.

    abstract::The aim of this review is to comment the results described in the literature concerning the possible pharmacodynamic mechanisms involved in the improvement of quality of life of angiotensin converting enzyme inhibitors that is just a working hypothesis. These drugs, widely used in the treatment of hypertension, preven...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Govantes C,Marín J

    更新日期:1996-01-01 00:00:00

  • KATP channels modulate GABA release in hippocampal slices in the absence of glucose.

    abstract::We studied the effects of KATP channel blockers on [3H]GABA release in the absence of glucose in rat hippocampal slices. The omission of glucose induced a marked increase in the efflux of [3H]GABA, which was antagonized by TTX (1 microM), but not by MK 801 (1 microM) or DNQX (100 microM). Glibenclamide (10-100 microM)...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Margaill I,Miquet JM,Doble A,Blanchard JC,Boireau A

    更新日期:1992-01-01 00:00:00

  • Erythropoietin and myocardial protection: what's new?

    abstract::Erythropoietin (EPO), the principal hematopoietic cytokine produced by the kidney and the liver in fetuses, regulates mammalian erythropoiesis and exhibits diverse cellular effects in non-hematopoietic tissues. The introduction of recombinant human EPO (rhEPO) has marked a significant advance in the management of anem...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Joyeux-Faure M,Godin-Ribuot D,Ribuot C

    更新日期:2005-08-01 00:00:00

  • Preclinical assessment of a new transdermal delivery system for clonidine (M-5041T).

    abstract::The effects of a new transdermal delivery system for clonidine (M-5041T) on hypotensive effect, urine volume, plasma renin activity (PRA) and antidiuretic hormone (ADH) in spontaneously hypertensive rats (SHRs) were compared to the effects of the continuous infusion of clonidine. Both M-5041T (1.5 and 4.5 mg/kg) and t...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Naruse T,Ishida T,Ishii R,Tagawa T

    更新日期:1996-01-01 00:00:00

  • Pharmacotherapy of polycystic ovary syndrome--an update.

    abstract::Polycystic ovary syndrome (PCOS) is a persisting challenge to clinical and basic research scientists as none of the presently available medications have been fully able to combat these consequences. The aim of the present review is to summarize the different lines of treatment available for the different symptomologie...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Saha L,Kaur S,Saha PK

    更新日期:2012-02-01 00:00:00

  • Effect of peptidyl-dipeptidase inhibitors in experimental convulsions in mice.

    abstract::The anticonvulsant effect of compounds that inhibit peptidyl-dipeptidase (PDP) on bicuculline (BIC)- and strychnine (STRYC)-induced seizures was assessed after intracerebroventricular (ICV) or intraperitoneal (IP) administration in Swiss albino mice. STRYC-induced seizures were delayed by ICV injections and high IP do...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Miñano FJ,Serrano JS,Sancibrián M,Serrano MI

    更新日期:1987-01-01 00:00:00

  • Higher intake of medications for digestive disorders in children prenatally exposed to drugs with atropinic properties.

    abstract::Childhood digestive disorders are a common occurrence and are sometimes unexplained. Maternal medication during the development of the foetus' digestive system may contribute to the increase in childhood digestive disorders, especially with drugs acting on the cholinergic system. This study investigated the associatio...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Benevent J,Hurault-Delarue C,Araujo M,Montastruc F,Montastruc JL,Lacroix I,Damase-Michel C

    更新日期:2019-06-01 00:00:00

  • The possible prophylactic effect of Nigella sativa seed extract in asthmatic patients.

    abstract::In previous studies, the relaxant, anticholinergic (functional antagonism) antihistaminic, effects of Nigella sativa have been demonstrated on guinea-pig tracheal chains. In the present study, the prophylactic effect of boiled extract of N. sativa on asthmatic disease was examined. Twenty-nine asthmatic adults were ra...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,随机对照试验


    authors: Boskabady MH,Javan H,Sajady M,Rakhshandeh H

    更新日期:2007-10-01 00:00:00

  • Tropisetron and paracetamol association in post-operative patients.

    abstract::Studies in animals and in healthy volunteers have demonstrated the central serotonergic analgesic mechanism of action of paracetamol involving the inhibition of this analgesia by tropisetron, a 5-HT3 antagonist. This randomized, double-blind, controlled study aims at studying this interaction in post-operative patient...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,随机对照试验


    authors: Pickering G,Faure M,Commun F,de Boissy EC,Roche G,Mom T,Simen E,Dubray C,Eschalier A,Gilain L

    更新日期:2012-06-01 00:00:00

  • Centrally acting antihypertensives change the psychogenic cardiovascular reactivity.

    abstract::Clonidine (CL) and Rilmenidine (RI) are among the most frequently prescribed centrally- acting antihypertensives. Here we compared CL and RI effects on psychogenic cardiovascular reactivity to sonant, luminous, motosensory, and vibrotactile stimuli during neurogenic hypertension. The femoral artery and vein of Wistar ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Mendonça MM,Costa AN,Moraes GCA,Martins GM,Almeida AF,Rincon GCN,Siqueira JPR,Padilha DM,Moya MI,Ferreira-Neto ML,Gomes RM,Pedrino GR,Fontes MAP,Colombari E,Crestani CC,Fajemiroye JO,Xavier CH

    更新日期:2021-01-19 00:00:00

  • Fish oils in the care of coronary heart disease patients: a meta-analysis of randomized controlled trials.

    abstract::What is the place of fish oils in the care of coronary heart disease (CHD) patients? As several clinical trials have already addressed this question without giving definitive answers, we did a meta-analysis of trials regarding the efficacy of omega-3 fatty acids in preventing cardiovascular mortality and morbidity. We...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,meta分析


    authors: Yzebe D,Lievre M

    更新日期:2004-10-01 00:00:00

  • Electrophysiology of ion channels of the heart.

    abstract::The contribution of Na+, Ca2+, and various K+ currents to the shape of the cardiac action potential is outlined based on the relation between electrophysiological properties and structure of channel molecules. These currents have also been found in human ventricular myocytes, where the most prominent K+ current is a t...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Ravens U,Wettwer E,Ohler A,Amos GJ,Mewes T

    更新日期:1996-01-01 00:00:00

  • Does rat global transient cerebral ischemia serve as an appropriate model to study emotional disturbances?

    abstract::We used two validated psychopharmacological methods, the forced swimming test (FST 20 min and 5 min) and the elevated plus-maze (EPM), to quantify depression-like and anxiety-like behavior induced by transient global cerebral ischemia in the rat. We also validated use of these methods for the study of antidepressant (...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Bantsiele GB,Bentué-Ferrer D,Amiot N,Allain H,Bourin M,Reymann JM

    更新日期:2004-12-01 00:00:00

  • Compared peripheral vascular responses to intravenous and intra-arterial administrations of positive inotropic agents in conscious dogs.

    abstract::In addition to their direct effects on cardiac contractility, a number of positive inotropic agents also induce, through direct peripheral vasodilation, a reduction in afterload which is of major importance in their beneficial effects in the treatment of congestive heart failure. However, the induced increase in cardi...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Gosgnach M,Gérard JL,Berdeaux A,Giudicelli JF

    更新日期:1991-01-01 00:00:00

  • Pharmacology and drug development in rare diseases: the attractiveness and expertise of the French medical pharmacology.

    abstract::Developing drugs for rare disease can be challenging due to specific rare disease characteristics. The French Medical Pharmacology is structured and positioned to play a major role in orphan drug research and development due to the required expertise concentrated into pharmacology departments, exclusively implemented ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Micallef J,Boutouyrie P,Blin O

    更新日期:2017-12-01 00:00:00

  • High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid.

    abstract::Oxidative stress and associated complications are the major pathological concerns of diabetic cardiomyopathy (DC). We aim to elucidate the mechanisms by which high glucose (HG) induced alteration in calcium homeostasis and evaluation of the beneficial effect of two concentrations (10 and 25 μm) of ferulic acid (FA). H...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Salin Raj P,Swapna SUS,Raghu KG

    更新日期:2019-08-01 00:00:00

  • Anandamide: an update.

    abstract::Endocannabinoid system has attracted the researchers to investigate into its ever fascinating roles governing many of the physiological functions in the human body. The prime endogenous cannabinoids are arachidonoylethanolamide also called anandamide and 2-arachidonoylglycerol. Recent pharmacological advances point ou...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Smita K,Sushil Kumar V,Premendran JS

    更新日期:2007-02-01 00:00:00

  • Prostaglandin E synthase in the pathophysiology of arthritis.

    abstract::Prostaglandin E synthase (PGES) is a recently identified terminal enzyme that acts downstream of cyclooxygenase and catalyzes the conversion of prostaglandin (PG) H2 to PGE2. At least three isozymes have been cloned so far, which are called membrane-associated PGES (mPGES)-1, mPGES-2, and cytosolic PGES. Among them, m...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Kojima F,Kato S,Kawai S

    更新日期:2005-06-01 00:00:00

  • Similia similibus obscurantur: the pharmacological clinical activity bias I. A prototype model to correct a disease-drug interaction leading to misestimations of drug-attributable side effects.

    abstract::Drugs have side effects that manifest as signs or symptoms which are sometimes undistinguishable from signs or symptoms of active disease. The conventional approximation of the rate of side effects of drugs is by subtracting the rate of signs and symptoms in the placebo group from that in the drug group. This measures...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Bernheim JL,Vrana I

    更新日期:1995-01-01 00:00:00

  • French adaptation and preliminary validation of a questionnaire to evaluate understanding of informed consent documents in phase I biomedical research.

    abstract::The content of informed consent documents (ICD) is a crucial element in the process of providing information to participants in biomedical research. Clear comprehension of the information, i.e. the ability to understand its meaning and its consequences, is of utmost importance. The objective of this study was to descr...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Paris A,Cornu C,Auquier P,Maison P,Radauceanu A,Brandt C,Salvat-Melis M,Hommel M,Cracowski JL

    更新日期:2006-02-01 00:00:00

  • Study of in vitro glucuronidation of hydroxyquinolines with bovine liver microsomes.

    abstract::Glucuronidation of drugs by UDP-glucuronosyltransferase (UGT) is a major phase II conjugation reaction. Defects in UGT are associated with Crigler-Najjar syndrome and Gilbert's syndrome with severe hyperbilirubinaemias and jaundice. We analysed the reactivities of some hydroxyquinoline derivatives, which are naturally...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Kanou M,Saeki K,Kato TA,Takahashi K,Mizutani T

    更新日期:2002-12-01 00:00:00