Enhanced transient receptor potential channel-mediated Ca2+ influx in the cells with phospholipase C-δ1 overexpression: its possible role in coronary artery spasm.


:We reported that coronary spasm was induced in the transgenic mice with the increased phospholipase C (PLC)-δ1 activity. We investigated the effect of enhanced PLC-δ1 on Ca2+ influx and its underlying mechanisms. We used human embryonic kidney (HEK)-293 and coronary arteries smooth muscle cells (CASMC). Intracellular free Ca2+ concentration ([Ca2+ ]i ; nm) was measured by fura-2, and Ca2+ influx was evaluated by the increase in [Ca2+ ]i after addition of extracellular Ca2+ . Acetylcholine (ACh) was used to induce Ca2+ influx. ACh-induced peak Ca2+ influx was 19 ± 3 in control HEK-293 cells and 71 ± 8 in the cells with PLC-δ1 overexpression (P < 0.05 between two groups). Nifedipine partially suppressed this Ca2+ influx, whereas either 2-APB or knockdown of classical transient receptor potential channel 6 (TRPC6) blocked this Ca2+ influx. In the human CASMC, ACh-induced peak Ca2+ influx was 29 ± 6 in the control and was increased to 45 ± 16 by PLC-δ1 overexpression (P < 0.05). Like HEK-293 cells, pretreatment with nifedipine partially suppressed Ca2+ influx, whereas either 2-APB or knockdown of TRPC6 blocked it. ACh-induced Ca2+ influx was enhanced by PLC-δ1 overexpression, and was blocked partially by nifedipine and completely by 2-APB. TRPC-mediated Ca2+ influx may be related to the enhanced Ca2+ influx in PLC-δ1 overexpression.


Fundam Clin Pharmacol


Murakami K,Osanai T,Tanaka M,Nishizaki K,Kinjo T,Tanno T,Ishida Y,Suzuki A,Endo T,Tomita H,Okumura K




Has Abstract


2017-08-01 00:00:00












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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Priyadharsini RP

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  • C.B-17 SCID mice develop epicardial calcinosis with unaltered cardiac function.

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    authors: Raghunathan S,Reynolds CL,Schwartz RJ,Stewart MD,McConnell BK

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  • Intracoronary infusion of bradykinin: effects on noradrenaline overflow following reperfusion of ischemic myocardium in the anesthetized dog.

    abstract:OBJECTIVE:The aim of this study was to investigate the effects of intracoronary bradykinin (BK) infusion on noradrenaline release and ventricular arrhythmias induced by coronary occlusion and reperfusion in the anesthetized dog. METHODS:14 anesthetized adult mongrel dogs of either sex underwent a 60 min occlusion of t...

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    authors: Ribuot C,Yamaguchi N,Godin D,Jetté L,Adam A,Nadeau R

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  • Anticoagulants for secondary prevention after acute myocardial infarction: lessons from the past decade.

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    pub_type: 杂志文章,评审


    authors: Atar D,Bode C,Stuerzenbecher A,Verheugt FW

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  • Anandamide: an update.

    abstract::Endocannabinoid system has attracted the researchers to investigate into its ever fascinating roles governing many of the physiological functions in the human body. The prime endogenous cannabinoids are arachidonoylethanolamide also called anandamide and 2-arachidonoylglycerol. Recent pharmacological advances point ou...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Smita K,Sushil Kumar V,Premendran JS

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  • Influence of landiolol on the dose requirement of propofol for induction of anesthesia.

    abstract::It was reported that the pharmacokinetics of propofol was influenced by cardiac output (CO). The purpose of this study was to evaluate the effect of landiolol (short-acting beta-1-adrenergic blocker) on the dose requirement of propofol for induction of anesthesia. Forty patients were randomly allocated to the control ...

    journal_title:Fundamental & clinical pharmacology

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    authors: Takizawa D,Saito S,Sato E,Hiraoka H,Kunimoto F,Goto F

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  • Plasma antioxidative activity during atorvastatin and fluvastatin therapy used in coronary heart disease primary prevention.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 临床试验,杂志文章,随机对照试验


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    更新日期:2004-02-01 00:00:00

  • Neuropharmacological effects of lipoic acid and ubiquinone on δ-aminolevulinic dehydratase, Na(+) , K(+) -ATPase, and Mg(2+) -ATPase activities in rat hippocampus after pilocarpine-induced seizures.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: de Freitas RM,Feng D,Jordán J

    更新日期:2011-04-01 00:00:00

  • Cholinesterase activity in pig airways and epithelial cells.

    abstract::Acetylcholinesterase (AChE, EC and butyrylcholinesterase (BChE, EC activities were detected in bronchial and bronchial epithelial cell homogenates of the pig. In the bronchial homogenates, the maximal upstroke velocity (Vmax) of AChE and the maximal velocity after second substrate fixation (Vss) of B...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Taisne C,Norel X,Walch L,Labat C,Verriest C,Mazmanian GM,Brink C

    更新日期:1997-01-01 00:00:00

  • The mechanisms of vasorelaxant effect of leptin on isolated rabbit aorta.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Sahin AS,Bariskaner H

    更新日期:2007-12-01 00:00:00

  • Pharmacokinetics of dextromoramide in surgical patients.

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    pub_type: 杂志文章


    authors: Pagani I,Barzaghi N,Crema F,Perucca E,Ego D,Rovei V

    更新日期:1989-01-01 00:00:00

  • Lacosamide improves biochemical, genotoxic, and mitochondrial parameters after PTZ-kindling model in mice.

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    pub_type: 杂志文章


    authors: Lazzarotto L,Pflüger P,Regner GG,Santos FM,Aguirre DG,Brito VB,Moura DJ,Dos Santos NM,Picada JN,Parmeggiani B,Frusciante MR,Leipnitz G,Pereira P

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  • Compared peripheral vascular responses to intravenous and intra-arterial administrations of positive inotropic agents in conscious dogs.

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    pub_type: 杂志文章


    authors: Gosgnach M,Gérard JL,Berdeaux A,Giudicelli JF

    更新日期:1991-01-01 00:00:00

  • Lithium decreased endothelium-mediated, but not nonadrenergic noncholinergic, relaxation of guinea pig corpus cavernosum in vitro: a role for nitrergic system.

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    authors: Ghasemi M,Farrokhi-Khajeh-Pasha Y,Ostovaneh MR,Dehpour AR

    更新日期:2011-04-01 00:00:00

  • Experimental and clinical methods in the development of anti-Alzheimer drugs.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Allain H,Bentué-Ferrer D,Zekri O,Schück S,Lebreton S,Reymann JM

    更新日期:1998-01-01 00:00:00

  • Trolox-derivative antioxidant protects against methanol-induced damage.

    abstract::This paper reports data on the effect of a new antioxidant, U-83836E, on the lipid peroxidation and antioxidant status of liver, red blood cells (RBCs) and blood serum of rats intoxicated with methanol (3.0 g/kg body weight). Methanol administration slightly increased the levels of peroxidation products in the liver, ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Skrzydlewska E,Farbiszewski R

    更新日期:1997-01-01 00:00:00

  • Choice of designs and doses for early phase trials.

    abstract::This paper reviews state-of-art statistical designs for dose-escalation procedures in first-into-man studies. The main focus will be on studies in oncology, as most statistical procedures for phase I trials have been proposed in this context. Extensions to situations such as the observation of bivariate outcomes and h...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Zhou Y

    更新日期:2004-06-01 00:00:00

  • Ameliorative effect of statin therapy on oxidative damage in heart tissue of hypercholesterolemic rabbits.

    abstract::The aim of this study was to investigate the effects of a high-cholesterol diet in the presence and absence of statin on Cu-Zn-superoxide dismutase (Cu,Zn-SOD), malondialdehyde (MDA), protein carbonyl (PCO), and nitric oxide (NO) of blood and heart tissue, the antioxidant activity of serum paraoxonase-1 (PON-1), and o...

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    authors: Sozer V

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  • Beraprost sodium-fluindione combination in healthy subjects: pharmacokinetic and pharmacodynamic aspects.

    abstract::Beraprost sodium (BPS), an orally active PGI2 (prostaglandine 12) analogue possesses vasodilatating and platelet aggregation inhibiting properties. It is being developed in peripheral arterial occlusive disease. As in future clinical practice BPS might be co-prescribed with oral anticoagulants, we investigated its int...

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    authors: Warot D,Berlin I,Aymard G,Ankri A,Fabry C,Besse B,Lechat P,Diquet B

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  • Pharmacological effects of primaquine ureas and semicarbazides on the central nervous system in mice and antimalarial activity in vitro.

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    authors: Kedzierska E,Orzelska J,Perković I,Knežević D,Fidecka S,Kaiser M,Zorc B

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  • Sulforaphane protects SK-N-SH cells against antipsychotic-induced oxidative stress.

    abstract::Adverse reactions to antipsychotic drugs (APs) have been attributed to oxidative stress. Sulforaphane (SF) is a potent antioxidant that protects against dopaminergic cell death. We examined the protective properties of SF against AP-induced oxidative stress in dopaminergic neuroblastoma cells. Human neuroblastoma SK-N...

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    authors: Mas S,Gassó P,Trias G,Bernardo M,Lafuente A

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  • Effects of oxazepam and acetaminophen on cicletanine metabolism in rat hepatocytes and liver microsomes.

    abstract::Cicletanine, a racemic furopyridine derivative synthesized as racemate, is used as an antihypertensive agent. Its two enantiomers are involved in the pharmacological effects of the drug. Cicletanine is metabolized by conjugation enzyme systems (phase II) into sulfoconjugated or glucuroconjugated enantiomers. As oxazep...

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    authors: Menard C,Lamiable D,Vistelle R,Droy-Lefait MT,Ratanasavanh D

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  • Long-term protection of obese Zucker rat kidneys from fibrosis and renal failure with an angiotensin-converting enzyme inhibitor/diuretic combination.

    abstract::Some combinations of antihypertensive agents were shown to reduce proteinuria in patients with renal failure. However, preventive effects of such combinations on renal structure and function are presently unknown when treatment is administered before the onset of renal abnormalities. We thus investigated the long-term...

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    authors: Renaud IM,Chainey A,Belair MF,Mandet C,Michel O,Myara I,Chevalier J,Plante GE

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  • Quantitative evaluation of the combination between cytotoxic drug and efflux transporter inhibitors based on a tumour growth inhibition model.

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    authors: Sostelly A,Payen L,Guitton J,Di Pietro A,Falson P,Honorat M,Boumendjel A,Gèze A,Freyer G,Tod M

    更新日期:2014-04-01 00:00:00

  • Combined therapy in the treatment of dyslipidemia.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Reiner Z

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    abstract::This study aimed to investigate the modulation of nitric oxide/reactive oxygen species in sodium nitroprusside relaxation in mice aorta. Sodium nitroprusside induced relaxation in endothelium-intact (e+) and endothelium-denuded (e-) aortas with greater potency in e+ than in e-. The nitric oxide synthase inhibitor did ...

    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Kangussu LM,Olivon VC,Arifa RD,Araújo N,Reis D,Assis MT,Soriani FM,de Souza Dda G,Bendhack LM,Bonaventura D

    更新日期:2015-04-01 00:00:00

  • Physiological and metabolic actions of mycophenolate mofetil on cultured newborn rat cardiomyocytes in normoxia and in simulated ischemia.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Bès S,Tatou E,Vandroux D,Tissier C,Rochette L,Athias P

    更新日期:2004-06-01 00:00:00

  • Underlying mechanism of penehyclidine hydrochloride on isolated rat uterus.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章


    authors: Xiao HT,Liao Z,Meng XM,Yan XY,Chen SJ,Mo ZJ

    更新日期:2009-08-01 00:00:00

  • Effect of simvastatin on the antihypertensive activity of losartan in hypertensive hypercholesterolemic animals and patients: role of nitric oxide, oxidative stress, and high-sensitivity C-reactive protein.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 临床试验,杂志文章


    authors: Abdel-Zaher AO,Elkoussi AE,Abudahab LH,Elbakry MH,Elsayed EA

    更新日期:2014-06-01 00:00:00

  • Nitric oxide and the control of renin secretion.

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    journal_title:Fundamental & clinical pharmacology

    pub_type: 杂志文章,评审


    authors: Reid IA,Chiu YJ

    更新日期:1995-01-01 00:00:00