Abstract：
BACKGROUND:Advanced glycation end products (AGEs) accumulate in patients with diabetes, particularly at sites of vascular damage and within atherosclerotic lesions, but whether they have direct actions on vascular smooth muscle cells (VSMCs) is controversial. METHODS:AGEs were constructed and characterized by protein content, level of modification, fluorescence, and molecular size. Human VSMCs were derived from different vascular beds. Glucose consumption, de novo protein synthesis, and proteoglycan biosynthesis were measured using a colorimetric assay and metabolic radiolabeling. Receptor for AGEs (RAGE) expression was assessed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. RESULTS:Treatment with AGEs under low or high glucose conditions showed no change in cellular glucose consumption or in cellular protein synthesis under low glucose conditions. Treatment of VSMCs with Nepsilon-(carboxymethyl)lysine in the presence of low glucose increased [35S]-sulfate incorporation into secreted proteoglycans by 72% (P < 0.001) and 67% (P < 0.001); however, the control proteins also increased [35S]-sulfate incorporation into proteoglycans by 56% (P < 0.01), with similar effects observed under high glucose conditions. Human VSMCs showed no difference in response to glycated and non-glycated protein. Protein and gene expression of RAGE in VSMC was approximately 50-fold lower compared to HMEC-1 and U937 cells, consistent with the immunohistochemical staining of RAGE in vivo. CONCLUSION:VSMCs show very low levels of RAGE expression; thus, activation of VSMCs by AGEs does not occur. In diabetes, RAGE expression in VSM may increase to the extent that it becomes activated by AGEs in a manner that would contribute to the process of atherosclerosis.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Ballinger ML,Thomas MC,Nigro J,Ivey ME,Dilley RJ,Little PJdoi
10.1111/j.1523-1755.2005.00746.xsubject
Has Abstractpub_date
2005-12-01 00:00:00pages
2756-65issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)51182-4journal_volume
68pub_type
杂志文章abstract：BACKGROUND:Approximately 8% of autosomal-dominant polycystic kidney disease (ADPKD) patients have intracranial aneurysms. The risk of growth and rupture of those discovered by presymptomatic screening is key to the feasibility and success of a screening program. This study was initiated to ascertain this risk. METHODS...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2004.00572.x
更新日期：2004-05-01 00:00:00
abstract：:Because of high risk of cardiovascular disease, patients with chronic kidney disease may benefit from cholesterol-lowering therapy beyond statins. A cost-effectiveness analysis of adding ezetimibe to high-dose statins for primary cardiovascular disease prevention in patients with non-dialysis-dependent chronic kidney ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2019.02.029
更新日期：2019-07-01 00:00:00
abstract：:Diagnosis and treatment of atherosclerotic renal artery stenosis (ARAS) in the elderly is a never-ending challenge for every nephrologist. Kalra et al. report that adjusted hazard ratios for ARAS increased threefold from 1992 to 2004 in 16 million United States Renal Data System participants aged 66 years or older. Ho...
journal_title：Kidney international
pub_type： 信件
doi：10.1038/ki.2009.326
更新日期：2010-01-01 00:00:00
abstract：:Vascular calcification is recognized as a major contributor to cardiovascular disease (CVD) in end stage renal disease (ESRD) patients. Susceptibility to vascular calcification is genetically determined and actively regulated by diverse inducers and inhibitors. One of these inducers, hyperphosphatemia, promotes vascul...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2008.644
更新日期：2009-05-01 00:00:00
abstract：BACKGROUND:Hypertension is a recognized complication of partial correction of anemia with recombinant human erythropoietin (epoetin) in hemodialysis patients. We used interdialytic ambulatory blood pressure (ABP) monitoring to study the effects of partially corrected anemia versus normal hematocrit (hct) on BP in hemod...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.1999.00531.x
更新日期：1999-07-01 00:00:00
abstract：:Cherney and co-workers examine the functional phenotype of the G894T eNOS polymorphism in a selected group of apparently healthy subjects. The investigators used L-arginine infusion and induced changes in renal hemodynamics. While L-arginine increased effective plasma flow and glomerular filtration rate in GG subjects...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2008.605
更新日期：2009-02-01 00:00:00
abstract：:We explored how B-lymphocytes influence in vitro T-cell alloresponses in patients with antibody-mediated rejection (AMR), testing whether B-cells would be preferentially involved in this group of patients. Peripheral blood mononuclear cells were collected from 65 patients having biopsy: 14 patients with AMR and 5 with...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.100
更新日期：2015-09-01 00:00:00
abstract：:The hormonal responsiveness profile of the cortical collecting duct varies from one species to another. To identify the hormones and agonists that modulate the functions of this tubule segment in the human species, we generated a cell line (HCD) immortalized by SV40 virus. The tubular origin of this cell line was asse...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.186
更新日期：1995-05-01 00:00:00
abstract：:Nitric oxide is generated from L-arginine by nitric oxide synthase (NOS), an enzyme that exists in several isoforms. Some studies found that a polymorphism (G894T) in the endothelial NOS gene was associated with decreased nitric oxide bioactivity and vascular complications. However, it is not known whether the enzyme ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.574
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:Mast cells are growth factor-rich, bone marrow-derived cells that infiltrate injured tissue where they have been implicated in the pathogenesis of progressive fibrosis. METHODS:Mast cell infiltration and the expression of related chemoattractants was examined following 5/6 nephrectomy, a model of progressiv...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00183.x
更新日期：2003-09-01 00:00:00
abstract：:Isotomic fluid reabsorption (JV) of rat renal proximal tubules was examined by the shrinking droplet method in combination with simulatneous perfusion of blood capillaries. Sensitivity of JV measurement was improved by using each punctured tubule for control measurements: 1) Parathyroid hormoen (PTH) on the contralumi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1977.12
更新日期：1977-02-01 00:00:00
abstract：:During vasculitis, activation of the kinin system induces inflammation, whereby the kinin B1-receptor is expressed and activated after ligand binding. Additionally, activated blood cells release microvesicles into the circulation. Here we determined whether leukocyte-derived microvesicles bear B1-kinin receptors durin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.09.023
更新日期：2017-01-01 00:00:00
abstract：:Acute kidney injury (AKI) occurs in about half of patients in septic shock and the mortality of AKI with sepsis is extremely high. An effective therapeutic intervention is urgently required. Statins are 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors that also have pleiotropic actions. They have been report...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000300
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:The characteristic feature of Goodpasture disease is the occurrence of an autoantibody response to the noncollagenous domain of the alpha3 chain of type IV collagen [alpha3(IV)NC1] in the alveolar and glomerular basement membrane. These antibodies are associated with the development of a rapidly progressive ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1999.055003936.x
更新日期：1999-03-01 00:00:00
abstract：:Methods for estimating glomerular volume were compared in Zenker-fixed, paraffin-embedded biopsies from 10 patients with insulin-dependent diabetes mellitus and 6 normal kidney donors. Two methods of measurement of individual glomerular volumes were used: the Cavalieri method (considered the "gold standard") and the m...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.165
更新日期：1992-04-01 00:00:00
abstract：:Nephronophthisis is an autosomal recessive chronic tubulointerstitial disease that progresses to end-stage renal disease (ESRD) in about 10% of cases during infancy. Mutations in the INVS (NPHP2) gene were found in a few patients with infantile nephronophthisis. Mutations of NPHP3, known to be associated with adolesce...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.662
更新日期：2009-04-01 00:00:00
abstract：:Many xenobiotics including the pharmacoenhancer cobicistat increase serum creatinine by inhibiting its renal active tubular secretion without affecting the glomerular filtration rate. This study aimed to define the transporters involved in creatinine secretion, applying that knowledge to establish the mechanism for xe...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2014.66
更新日期：2014-08-01 00:00:00
abstract：:Because of differences in case-mix across states, state-level case-mix-adjusted end-stage renal disease (ESRD) incident rates are reported in each United States Renal Data System Annual Data Report to make the across-state comparisons valid. The adjusted rates were estimated by the direct adjustment method, a widely u...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5000299
更新日期：2006-04-01 00:00:00
abstract：BACKGROUND:The substitution of tryptophan for glycine at amino acid 460 (Gly460Trp polymorphism) of the alpha-subunit of the heterodimeric cytoskeleton protein adducin increases renal sodium reabsorption and may be involved in the pathophysiology of essential hypertension. In the present study, we investigated in multi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00691.x
更新日期：2002-12-01 00:00:00
abstract：:The primary goal of this study was to determine if the ability to undergo catch-up growth following a transient injury is preserved in an experimental model of moderate chronic renal failure (CRF) and the effect of growth hormone (GH) administration on such phenomenon. Young rats were subtotally nephrectomized (days 0...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5001949
更新日期：2006-12-01 00:00:00
abstract：:Dialysis providers in the United States may soon be held accountable for their patients' 30-day hospital readmissions. However, few studies have evaluated the timing of readmissions, which determines the window in which dialysis providers could act to prevent readmission. We therefore examined the timing of readmissio...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.03.025
更新日期：2017-10-01 00:00:00
abstract：:Interstitial monocytic infiltration of the kidney occurs within hours of acute kidney injury and is an important determinant of functional decline and fibrosis. Li et al. used several surface markers to distinguish between dendritic cells and inflammatory monocytes following acute kidney injury and to identify two che...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2008.555
更新日期：2008-12-01 00:00:00
abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia emerged in Wuhan, China in December 2019. Unfortunately, there is a lack of evidence about the optimal management of novel coronavirus disease 2019 (COVID-19), and even less is available in patients on maintenance hemodialysis therapy than in the g...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.04.031
更新日期：2020-07-01 00:00:00
abstract：:Class IV-S lupus nephritis is often associated with more necrosis and fewer subendothelial immune deposits compared to class IV-G lupus nephritis, suggestive of necrotising glomerular inflammation found in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. ANCAs are present in a significant proportion o...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.04.029
更新日期：2017-11-01 00:00:00
abstract：:A study of dialysis costs in five major dialysis centers throughout the United States was conducted in 1973. When available, home, home training, limited care, satellite and in-hospital-full-care dialysis were included. The study's objective was to determine the cost per dialysis for each separate dialysis modality. A...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1976.31
更新日期：1976-03-01 00:00:00
abstract：:We studied in 32 patients on maintenance hemodialysis (duration of treatment 6 to 133 months) whether duration of dialysis treatment affects blood pressure, plasma noradrenaline levels and alpha 2-adrenoceptor density (assessed in platelet membranes by 3H-yohimbine binding). Plasma noradrenaline levels were a signific...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1987.267
更新日期：1987-11-01 00:00:00
abstract：:Renal involvement in von Hippel-Lindau (VHL) disease has emerged as the most prevalent cause of death in this hereditary disorder. In a group of 43 VHL patients (23 unrelated families) with renal lesions we examined whether severity of renal disease is affected by parental inheritance and VHL subtype (1, without pheoc...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.395
更新日期：1996-09-01 00:00:00
abstract：:Antioxidants have been used as therapies to decrease oxidative stress and improve CVD risk in hemodialysis (HD) patients. A systematic search of the Medline database (search date 30 April 2011) found 56 studies investigating the effects of antioxidant therapies on biomarkers of oxidative stress (53 studies) or clinica...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2011.341
更新日期：2012-02-01 00:00:00
abstract：:When the endotoxin-lipopolysaccharide (LPS) derived from the cell wall of gram-negative bacteria is given to the rat in vivo, there are prompt, marked decreases in glomerular filtration rate (GFR), renal blood flow (RBF) and % Na+ reabsorption (%T-Na+). However, it has not been determined whether the endotoxin itself ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1990.105
更新日期：1990-05-01 00:00:00
abstract：:To determine whether acute renal failure (ARF) increases the long-term risk of progressive chronic kidney disease (CKD), we studied the outcome of patients whose initial kidney function was normal or near normal but who had an episode of dialysis-requiring ARF and did not develop end-stage renal disease within 30 days...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.289
更新日期：2009-10-01 00:00:00
