Abstract:
:Parathyroid hormone (PTH) and tumor necrosis factoralpha (TNFalpha) are bone resorptive agents that upregulate interleukin-6 (IL-6) and RANKL production by osteoblasts. IL-6 mRNA expression induced by PTH is rapid and transient in osteoblasts both in vitro and in vivo. This study found that IL-6 secretion induced by PTH is also rapid and transient. The induction of RANKL mRNA by PTH is also rapid and transient although with an extended time course compared to that of IL-6 mRNA. In contrast, the effects of TNFalpha are biphasic. During the first 2 h of stimulation with TNFalpha, the responses are similar to those induced by PTH. This is followed by a period of relatively low IL-6 and RANKL mRNA levels and little IL-6 secretion. A late phase of increased IL-6 and RANKL mRNA expression occurs 12-24 h after stimulation with TNFalpha leading to a significant increase in IL-6 secretion. A similar biphasic pattern of activation of p38 MAP kinase is induced by TNFalpha. p38alpha/beta activation is required for the increased RANKL mRNA during the early phase of stimulation by TNFalpha but not in the late phase. In contrast, p38alpha/beta activation is not required for increased IL-6 mRNA or IL-6 protein secretion in either the early or late phases of stimulation by TNFalpha. Blocking the increases in IL-6 transcription completely eliminates IL-6 secretion induced during the early phases of stimulation by either PTH or TNFalpha. Consistent with the dependence on transcription, IL-6 mRNA is rapidly degraded with half-lives of 10-14 min following stimulation with either PTH or TNFalpha. In contrast to IL-6, RANKL mRNA is substantially more stable with half-lives of 40-60 min. Taken together, our results show that TNFalpha and PTH utilize distinct mechanisms to induce IL-6 and RANKL expression with markedly different kinetics. The more extensive effect of TNFalpha likely reflects that TNFalpha stimulates IL-6 production and bone resorption in pathological situations. In contrast, the less extensive effect of PTH likely reflects that it acts in physiological situations where it is important to minimize the potential adverse effects of high levels of IL-6 on bone and/or surrounding tissues.
journal_name
Bonejournal_title
Boneauthors
Dai JC,He P,Chen X,Greenfield EMdoi
10.1016/j.bone.2005.10.007subject
Has Abstractpub_date
2006-04-01 00:00:00pages
509-20issue
4eissn
8756-3282issn
1873-2763pii
S8756-3282(05)00412-6journal_volume
38pub_type
杂志文章相关文献
BONE文献大全abstract::Dynamic mechanical loading increases bone density and strength and promotes osteoblast proliferation, differentiation and matrix production, by acting at the gene expression level. Molecular mechanisms through which mechanical forces are conversed into biochemical signalling in bone are still poorly understood. A grow...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.10.016
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Fibrodysplasia ossificans progressiva (FOP) is a rare, disabling genetic disorder characterized by episodic soft tissue swelling (flare-ups) that leads to progressive heterotopic ossification and restricted joint mobility. METHODS:Here we present the first longitudinal patient-reported mobility assessment (...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.005
更新日期:2018-04-01 00:00:00
abstract::Physician recommendation for prophylactic surgical fixation of a femur with metastatic bone disease (MBD) is usually based on Mirels' criteria and clinical experience, both of which suffer from poor specificity. This may result in a significant number of these health compromised patients undergoing unnecessary surgery...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.02.011
更新日期:2018-05-01 00:00:00
abstract::Osteoclastic bone degradation depends on the activity of several proteolytic enzymes, in particular to those belonging to the classes of cysteine proteinases and matrix metalloproteinases (MMPs). Yet, several findings suggest that the two types of plasminogen activators (PA), the tissue- and urokinase-type PA (tPA and...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.07.004
更新日期:2008-11-01 00:00:00
abstract::Vertebral fracture risk is a heritable complex trait. The aim of this study was to identify genetic susceptibility factors for osteoporotic vertebral fractures applying a genome-wide association study (GWAS) approach. The GWAS discovery was based on the Rotterdam Study, a population-based study of elderly Dutch indivi...
journal_title:Bone
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract::Ossification of the posterior longitudinal ligament of the spine (OPLL) is characterized by ectopic bone formation in the spinal ligaments. Mechanical stress, which acts on the posterior ligaments, is thought to be an important factor in the progression of OPLL. To elucidate this mechanism, we investigated the effects...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00204-7
更新日期:2003-10-01 00:00:00
abstract::How estrogen therapy influences bone metabolism in postmenopausal women has previously been studied using several approaches, including bone densitometry, measurement of biochemical markers of bone turnover, and histomorphometry. Taken together, these investigations suggest that conventional doses of estrogen protect ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/s8756-3282(98)00156-2
更新日期:1999-02-01 00:00:00
abstract::Vitamin D is well known for its role in promoting skeletal health. Vitamin D status is determined conventionally by circulating 25-dihydroxyvitamin D (25OHD) concentration. There is evidence indicating that circulating 25OHD concentration is affected by variation in Gc, the gene encoding the vitamin D binding protein ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.12.068
更新日期:2015-05-01 00:00:00
abstract::Osteonecrosis of the femoral head (ONFH) is a skeletal disorder characterized by ischemic deterioration, bone marrow edema and eventually femoral head collapse. The systemic regulation of ONFH in adult patients has not been examined. Serum proteomic is an innovative tool that potentially detects simultaneous expressio...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.04.019
更新日期:2008-09-01 00:00:00
abstract:PURPOSE:Low trauma rib fracture (hereinafter, rib fracture) is common in the elderly, but its risk factors and mortality consequence are rarely studied. We sought to define the epidemiology of rib fracture and the association between rib fracture and postfracture mortality. METHODS:The study was part of the Dubbo Oste...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.08.016
更新日期:2018-11-01 00:00:00
abstract::We previously reported that calcium intake enhanced the leg bone response to physical activity of preschool children in a 12-month randomized trial of calcium supplementation and physical activity. To determine whether the intervention-induced changes in leg bone mineral content and size were maintained through the su...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.bone.2004.08.012
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVE:The pathogenesis of osteonecrosis of the femoral head (ONFH) probably reflects multiple etiologies. Recent studies have explored associations between genetic mutations and/or polymorphisms and ONFH. Annexins (ANXs) have been implicated in many physiological functions, including blood coagulation, inflammation...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.03.670
更新日期:2009-07-01 00:00:00
abstract::Progressive osseous heteroplasia (POH) is a rare autosomal dominant disorder of mesenchymal differentiation characterized by progressive heterotopic ossification (HO) of dermis, deep connective tissues and skeletal muscle. Usually, initial bone formation occurs during infancy as primary osteoma cutis (OC) then progres...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.06.015
更新日期:2013-10-01 00:00:00
abstract::Mutations in the SCN8A gene encoding the neuronal voltage-gated sodium channel Nav1.6 are known to be associated with epileptic encephalopathy type 13. We identified a novel de novo SCN8A mutation (p.Phe360Ala, c.1078_1079delTTinsGC, Exon 9) in a 6-year-old girl with epileptic encephalopathy accompanied by severe juve...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.025
更新日期:2017-10-01 00:00:00
abstract::Approximately 5% of all military recruits incur stress fracture injuries during intense physical training, predominately in the lower extremity. We compared young men and women with stress fracture injury (subjects) to a matched group of uninjured volunteers (controls) during a summer training program at the United St...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.05.014
更新日期:2004-09-01 00:00:00
abstract::E-Series prostaglandins (PGE) are powerful osteogenic substances when given in vivo. The purpose of this work was to determine the ability of locally administered PGE1 to promote alveolar bone formation. Controlled-release pellets containing PGE1 were implanted on the lateral mandibular surface of Beagle dogs and tiss...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(93)90198-j
更新日期:1993-05-01 00:00:00
abstract::Two major mechanisms that could potentially be responsible for toughening in mineralized tissues, such as bone and dentin, have been identified-microcracking and crack bridging. While evidence has been reported for both mechanisms, there has been no consensus thus far on which mechanism plays the dominant role in toug...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.02.001
更新日期:2004-05-01 00:00:00
abstract::The influence of porosity, osteon density, osteonal area, osteonal lamellar area, osteon size, and haversian canal size on the tension and shear fracture toughness, that is, the mode I and mode II strain energy release rate (GIc and GIIc), respectively, were investigated for the human femur and the tibia. The results ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00173-7
更新日期:1997-11-01 00:00:00
abstract::Oral glucocorticoids (GC) preserve muscle strength and prolong walking in boys with Duchenne muscular dystrophy (DMD). Although vertebral fractures have been reported in boys taking GC, fracture rates for different GC regimes have not been investigated. The aim of this pragmatic longitudinal study was to compare growt...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.07.019
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVES:Progression of an incomplete atypical femoral fracture (IF) to a complete fracture in patients on long term bisphosphonate (BP) therapy can be catastrophic. We aimed to determine the frequency and imaging features of IF in asymptomatic patients on long-term BP therapy and to identify distinguishing clinical ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.03.018
更新日期:2013-07-01 00:00:00
abstract::The process of bone turnover displays variations over 24 h, with C-terminal cross-linked telopeptide of type 1 collagen (CTX) and osteocalcin exhibiting a nadir in the afternoon and a peak in the night. In contrast, N-terminal propeptide of type 1 procollagen (P1NP) did not display an apparent 24-hour rhythm. Other em...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.10.002
更新日期:2019-03-01 00:00:00
abstract::The purpose of this in vitro biomechanical study is to determine the force attenuation capacity of four different hip protectors (KPH1, KPH2, Safehip, and Safetypants) in falling simulations in elderly subjects (falls to the side). The simulated falling conditions were created by a biomechanical testing system, which ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00154-4
更新日期:1999-08-01 00:00:00
abstract::A computerised technique is described for the quantitative assessment of resorption cavities in iliac trabecular bone. Using an Ibas II image analyser, the original bone surface, eroded by bone resorbing cells, is reconstructed using a curve fitting technique that maintains a smooth continuity with the trabecular bone...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(90)90076-b
更新日期:1990-01-01 00:00:00
abstract::Osteopetrotic bone shows dissociation between bone mineral density (BMD) and bone strength. In this study, volumetric BMD; preferential orientation of the extracellular matrix (ECM), which is composed of collagen fibers and apatite crystals as bone material quality; and mechanical properties of the src-/- osteopetroti...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.023
更新日期:2017-10-01 00:00:00
abstract::Bisphosphonates increase bone mass and reduce fracture risk, but their anti-resorptive action may lead to increases in fatigue microdamage. To investigate how bisphosphonate effects influence changes in bone volume and microdamage in the long term, a strain-adaptive model of bone remodeling and microdamage balance was...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.007
更新日期:2004-07-01 00:00:00
abstract:INTRODUCTION:Studies on the association of vitamin D and bone mineral density (BMD) in adolescence and young adults have shown contrasting results. None of these studies have examined the course and baseline in vitamin D intake. The purpose of this study was to examine the association between baseline and the course of...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.10.055
更新日期:2009-03-01 00:00:00
abstract::Osteogenesis occurs in striking interaction with angiogenesis. There is growing evidence that endothelial cells are involved in the modulation of osteoblast differentiation. We hypothesized that primary human umbilical vein endothelial cells (HUVEC) should be able to modulate primary human osteoprogenitors (HOP) funct...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.01.025
更新日期:2008-06-01 00:00:00
abstract::Although low bone mineral density (BMD) is strongly associated with increased fracture risk, up to 50% of those who suffer fractures are not detected as high-risk patients by BMD testing. Thus, new approaches may improve identification of those at increased risk for fracture by in vivo assessment of altered bone tissu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.03.021
更新日期:2018-01-01 00:00:00
abstract::Popular psychotropic drugs, like the antidepressant selective serotonin reuptake inhibitors (SSRIs) and tricyclic antidepressants (TCAs), and the mood stabilizer lithium, may have skeletal effects. In particular, preclinical observations suggest a direct negative effect of SSRIs on the skeleton. A potential caveat in ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.12.031
更新日期:2010-04-01 00:00:00
abstract::Skeleton is the most common organ targeted by breast cancer cells, especially from estrogen receptor alpha (ER)-positive neoplasms. Metastatic cells can stimulate directly or indirectly osteoclast-mediated bone resorption. Tumor-induced osteolysis is often extensive and leads to the release of large quantities of calc...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.021
更新日期:2004-08-01 00:00:00