Abstract:
:The cell-surface receptor c-kit and its cognate ligand stem-cell factor (SCF) or steel factor (SLF) are important for the maintenance of hematopoiesis both in vitro and in vivo. Transforming growth factor-beta (TGF-beta) has been shown to be a potent inhibitor of SLF-mediated synergistic growth of murine Lin-Sca-1+ progenitor cells, as well as more committed progenitors. In the present study, we examined the regulation of c-kit mRNA and cell-surface expression by TGF-beta. Among the murine hematopoietic progenitor cells tested, the myeloid cell line FDC-P1 and the mast-cell line MC-6, as well as progenitor-enriched bone marrow cells, constitutively expressed functional cell-surface c-kit. Treatment of these progenitor cell lines and primary progenitor cells with TGF-beta resulted in downregulation of cell-surface c-kit expression. This effect was not a secondary event of cell-cycle status. TGF-beta inhibition was dose- and time-dependent, with 50% inhibition seen between 0.3 to 3 ng/mL TGF-beta and maximal inhibition at 30 ng/mL. Using the FDC-P1 cell line, we observed that the inhibition of cell-surface c-kit expression by TGF-beta is preceded by a marked reduction in c-kit mRNA levels starting 2 hours after TGF-beta treatment, and reaches a maximum by 6 hours. The inhibition in steady-state c-kit mRNA levels is explained, in part, by a decrease in the half-life of c-kit transcripts (2 to 4 hours for control cells v 0.5 to 1.5 hours for TGF-beta-treated cells). These findings suggest that TGF-beta regulates the responsiveness of murine hematopoietic progenitors to SLF through a decrease in c-kit message stability leading to decreased cell-surface expression.
journal_name
Bloodjournal_title
Bloodauthors
Dubois CM,Ruscetti FW,Stankova J,Keller JRsubject
Has Abstractpub_date
1994-06-01 00:00:00pages
3138-45issue
11eissn
0006-4971issn
1528-0020journal_volume
83pub_type
杂志文章相关文献
BLOOD文献大全abstract::In a previous multicenter phase III trial comparing peripheral blood stem cell transplantation (PBSCT) to bone marrow transplantation (BMT) from HLA-matched related donors, we found no statistically significant difference in the cumulative incidence of clinical extensive chronic graft-versus-host disease (GVHD) in the...
journal_title:Blood
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2002-01-0011
更新日期:2002-07-15 00:00:00
abstract::Bortezomib is a proteasome inhibitor with remarkable preclinical and clinical antitumor activity in multiple myeloma (MM) patients. The initial rationale for its use in MM was inhibition of nuclear factor (NF)-kappaB activity by blocking proteasomal degradation of inhibitor of kappaBalpha (IkappaBalpha). Bortezomib in...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-199604
更新日期:2009-07-30 00:00:00
abstract::Gaucher disease, caused by a deficiency of the lysosomal enzyme glucocerebrosidase, leads to prominent glucosylceramide accumulation in lysosomes of tissue macrophages (Gaucher cells). Here we show glucosylsphingosine, the deacylated form of glucosylceramide, to be markedly increased in plasma of symptomatic nonneuron...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-352971
更新日期:2011-10-20 00:00:00
abstract::X-linked severe combined immunodeficiency (XSCID) is characterized by absent or profoundly reduced numbers of T cells and normal numbers of B cells in the circulation. Affected patients have mutations of the interleukin-2 (IL-2) receptor gamma chain gene. Using Epstein-Barr virus-transformed B-lymphoblastoid cell line...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-08-15 00:00:00
abstract::Plasmodium vivax merozoites only invade reticulocytes, a minor though heterogeneous population of red blood cell precursors that can be graded by levels of transferrin receptor (CD71) expression. The development of a protocol that allows sorting reticulocytes into defined developmental stages and a robust ex vivo P vi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-08-596015
更新日期:2015-02-19 00:00:00
abstract::A large number of alterations in genes encoding receptor tyrosine kinase (RTK), namely FLT3, c-KIT, platelet-derived growth factor (PDGF) receptors, fibroblast growth factor (FGF) receptors, and the anaplastic large cell lymphoma kinase (ALK), have been found in hematopoietic malignancies. They have drawn much attenti...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-04-279752
更新日期:2010-10-07 00:00:00
abstract::Cervical internal carotid artery (cICA) occlusion is a recognized cause of acute ischemic stroke (AIS) in sickle cell disease (SCD), but the associated clinical and radiologic features are not well described. We reviewed data on cervical magnetic resonance angiography (cMRA) performed prospectively in 67 patients (55 ...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2011-03-337915
更新日期:2011-12-01 00:00:00
abstract::Potassium tellurite (K2TeO3) was found to be a potent antisickling agent that inhibited red cell sickling at concentrations less than 10 mumol/L. The inhibitory effect depended on the incubation time, with the effect increasing with longer incubation periods. Because tellurite causes swelling of red cells, and because...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::We analyzed the incidence of posttransplant chronic myelogenous leukemia (CML) relapse in 283 consecutive related-donor (n = 177) and unrelated-donor (n = 106) allogeneic transplant recipients. Twenty-two of 165 related-donor recipients with stable or advanced disease at the time of transplant had hematologic relapse ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-07-15 00:00:00
abstract::A 72-yr-old male with a lifelong history of easy bruisability and posttraumatic bleeding had a prolonged prothrombin time and activated partial thromboplastin time. His plasma Stypven, Taipan, and Echis carinatus venom clotting times were prolonged. The presence of a dysprothrombin was confirmed by the discrepancy bet...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8 [HHV-8]) is a herpesvirus linked to the development of Kaposi's sarcoma (KS), primary effusion lymphoma, and a proportion of Castleman's disease. KSHV encodes viral interleukin-6 (vIL-6), which is structurally homologous to human and muri...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-06-15 00:00:00
abstract::Thirty-four adult and pediatric hemophilia A and B patients and 50 nonhemophilic members belonging to 28 families were enrolled in August 1984 in a study of human T cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) antibody status and T cell subpopulation numbers. All 50 household contac...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Gadd45a has been involved in DNA damage response and in many malignancies, including leukemia. However, the function of Gadd45a in hematopoietic stem cells (HSCs) remains unknown. Here, we reported that Gadd45a-deficient (Gadd45a(-/-)) mice showed a normal hematologic phenotype under homeostatic conditions. However, f...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-05-504084
更新日期:2014-02-06 00:00:00
abstract::Fanconi anemia (FA) is a hereditary disorder caused by mutations in any 1 of 22 FA genes. The disease is characterized by hypersensitivity to interstrand crosslink (ICL) inducers such as mitomycin C (MMC). In addition to promoting ICL repair, FA proteins such as RAD51, BRCA2, or FANCD2 protect stalled replication fork...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003782
更新日期:2020-07-31 00:00:00
abstract::Concentrations of plasma fibrinopeptide A (FPA) were measured by radioimmunoassay in 50 patients with venous thromboembolism or disseminated intravascular coagulation or both. A consistent discrepancy was observed in values obtained with two anti-FPA antisera. Analysis of extracts from plasma of these patients by high...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::CD56(high) acute myeloid leukemias (AMLs) have a poor prognosis, but it has been unclear how CD56 expression is controlled and how it relates to clinical aggressiveness. We show that CD56 expression on AML cells correlates with an abnormal expression pattern of runt-related transcription factor 1 (RUNX1) isoforms. Whe...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-02-074203
更新日期:2007-09-15 00:00:00
abstract::Normal human bone marrow contains cells capable of forming colonies of hemopoietic cells in fibrin clots in diffusion chambers implanted intraperitoneally (i.p.) into irradiated mice. The present paper describes the proliferation of such colony-forming units (CFUD) in cultures in vivo. Cells harvested from diffusion c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::The combination of imatinib with chemotherapy has been recently reported as very promising in patients with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). During 2004 and 2005, 45 patients with newly diagnosed Ph+ ALL were treated in the Group for Research on Adult Acute Lymphoblastic Leuke...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2006-03-011908
更新日期:2007-02-15 00:00:00
abstract::Survivin, a member of the inhibitor of apoptosis protein family, is expressed in almost all types of malignancies, making this protein a useful tool for the development of broadly applicable vaccination therapies. We used a recently identified HLA-A2 binding peptide and dendritic cells (DCs) from healthy donors to ind...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-08-2554
更新日期:2003-07-15 00:00:00
abstract::Several patients with clinical features of chronic myeloid leukemia (CML) have fusion of the TEL (ETV6) gene on 12p13 with ABL on 9q34 and express a chimeric Tel-Abl protein that contains the same portion of the Abl tyrosine kinase fused to Tel, an Ets family transcription factor, rather than Bcr. In a murine retrovir...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2001-12-0244
更新日期:2002-06-15 00:00:00
abstract::Twenty-six patients with progressive Hodgkin's disease after conventional chemotherapy received intensive chemoradiotherapy and autologous bone marrow transplantation (ABMT); 19 also received additional involved-field radiotherapy. Twenty-one patients [81%, 95% confidence intervals (CI) 61% to 94%] attained complete (...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::We conducted a double-blind, randomized multicenter trial to determine whether the addition of mycophenolate mofetil (MMF) improves the efficacy of initial systemic treatment of chronic graft-versus-host disease (GVHD). The primary endpoint was resolution of chronic GVHD and withdrawal of all systemic treatment within...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2009-02-202937
更新日期:2009-05-21 00:00:00
abstract::The loss of body cell mass (bcm) in senescence and wasting is poorly understood. We now show that the plasma cystine/acid soluble thiol ratio, ie, an indicator of the redox state, is increased in old age and cancer patients and correlated with a decrease in bcm and plasma albumin. A cause/effect relationship was sugge...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1998-07-01 00:00:00
abstract::Some multilineage hemopoietic colonies contain, in addition to myeloid cells, T lymphocytes. These proliferate extensively in liquid suspension culture under the influence of a T cell growth factor provided by phytohemagglutinin-T cell-conditioned medium (PHA-TCM). The clonal origin of these myeloid and lymphoid compo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::T-cell receptor (TCR) beta-chain proteins appear early (approximately 15th week of gestation) during human thymic ontogenesis. These beta-chain proteins, which appear before terminal deoxynucleotidyl transferase (TdT), could be an expression of a fully rearranged (V-D-J), incompletely rearranged (D-J), or germline TCR...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1992-03-15 00:00:00
abstract::Hydroxyurea treatment of sickle cell mice improved their survival from pneumococcal pneumonia, counteracting the abnormally elevated inflammatory response and reducing invasion of bacteria into the bloodstream, through down regulation of E-selectin. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2011-12-397794
更新日期:2012-02-23 00:00:00
abstract::The rapid transformation of human blood platelets from inert discoid cells to spheroechinocytes that is induced by adenosine diphosphate (ADP) has been followed by right-angle light scattering intensity measurements using a laser light source and a sensitive photomultiplier. Two steps have been observed, and their rat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-10-01 00:00:00
abstract::Increasingly, allogeneic and even more often autologous bone marrow transplants are being done to correct a wide variety of diseases. In addition, autologous marrow transplants potentially provide an opportune means of delivering genes in transfected, engrafting stem cells. However, despite its widespread clinical use...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::To examine the possible receptor-ligand pairs mediating adhesion of activated and "unactivated" platelets to leukocytes and the kinetics of leukocyte-platelet binding, we developed a flow cytometric assay using isolated cell fractions to accurately measure heterotypic cell adhesion, including both total leukocyte-plat...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The biochemical basis for complement acting directly on antigen-presenting cells to enhance their function in T-cell stimulation has been unclear. Here we present evidence that engagement of C3a receptor (C3aR) on the surface of dendritic cells (DCs) leads to alterations in the level of intracellular cyclic adenosine ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-05-156646
更新日期:2008-12-15 00:00:00