Abstract:
:The biochemical basis for complement acting directly on antigen-presenting cells to enhance their function in T-cell stimulation has been unclear. Here we present evidence that engagement of C3a receptor (C3aR) on the surface of dendritic cells (DCs) leads to alterations in the level of intracellular cyclic adenosine monophosphate (cAMP), a potent negative regulator of inflammatory cytokines. C3aR activation-induced depression of cAMP was associated with enhanced capacity of DCs for antigen uptake and T-cell stimulation. Conversely, C3aR-deficient DCs showed elevation of cAMP and impaired properties for antigen uptake and immune stimulation. Similarities in the phenotype of C3-deficient and C3aR-deficient DCs suggest that local production of C3 with extracellular metabolism to C3a is an important driver of DC alterations in cAMP. The finding of a link between complement and adaptive immune stimulation through cAMP offers new insight into how innate and adaptive immunity combine to generate efficient effector and memory responses.
journal_name
Bloodjournal_title
Bloodauthors
Li K,Anderson KJ,Peng Q,Noble A,Lu B,Kelly AP,Wang N,Sacks SH,Zhou Wdoi
10.1182/blood-2008-05-156646subject
Has Abstractpub_date
2008-12-15 00:00:00pages
5084-94issue
13eissn
0006-4971issn
1528-0020pii
blood-2008-05-156646journal_volume
112pub_type
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