Abstract：
:Normal human bone marrow contains cells capable of forming colonies of hemopoietic cells in fibrin clots in diffusion chambers implanted intraperitoneally (i.p.) into irradiated mice. The present paper describes the proliferation of such colony-forming units (CFUD) in cultures in vivo. Cells harvested from diffusion chambers after 1-14 days of culture in 450-R irradiated mice contained CFUD, which formed neutrophilic, eosinophilic, or megakaryocytic colonies when tested by secondary culture in fibrin clot chambers. When bone marrow was precultured in irradiated mice at a concentration of 10(6) cells per chamber, an initial fall in the number of neutrophilic CFUD was observed. This decrease was followed by an increase to a maximum at day 2, and then a secondary decrease. The number of neutrophilic CFUD recovered after 2 days of preculture in irradiated mice varied between 60% and 250% of the number present before preculture. Preculture in nonirradiated mice resulted in a significantly lower recovery of neutrophilic CFUD. In vitro treatment of bone marrow cells with hydroxyurea (OHU) after 2 days of preculture in irradiated mice resulted in a 68% +/- 5% reduction in the number of neutrophilic CFUD. In contrast, OHU had no similar effect on precultures from nonirradiated mice. Both the recovery and sensitivity to OHU of eosinophilic CFUD were independent of host irradiation. Similarly, no effect of host irradiation on the recovery or the 3H-thymidine (3HTdR) labeling index of morphologically recognizable granulocytic cells was observed at day 2. The data suggest an effect of humoral host factor(s) on the proliferation of early precursor cells, which are or become committed to differentiate into the neutrophilic pathway in diffusion chambers.
journal_name
Bloodjournal_title
Bloodauthors
Jacobsen Nsubject
Has Abstractpub_date
1977-03-01 00:00:00pages
415-24issue
3eissn
0006-4971issn
1528-0020journal_volume
49pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Hematopoietic stem cells (HSCs) show pronounced heterogeneity in self-renewal and differentiation behavior, which is reflected in their repopulation kinetics. Here, a single-cell-based mathematical model of HSC organization is used to examine the basis of HSC heterogeneity. Our modeling results, which are based on the...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-05-155374
更新日期：2008-12-15 00:00:00
abstract：:Precursor B-cell acute lymphoblastic leukemias (B-ALLs) have been shown to be oligoclonal at the Ig heavy-chain (IgH) gene level in up to 40% of cases by Southern blot hybridization. In contrast, oligoclonality as deduced from diversity of T-cell receptor (TcR)-delta gene rearrangements of the immature types (ie, V de...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-05-15 00:00:00
abstract：:Somatic mutation in immunoglobulin variable (V) region genes occurs largely in the germinal center and, after neoplastic transformation, imprints V genes of B-cell tumors with the mutational history of the cell of origin. Recently, it has been found that chronic lymphocytic leukemia (CLL) consists of 2 subsets, each w...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Osteoporosis is a frequent problem in disorders characterized by iron overload, such as the thalassemias and hereditary hemochromatosis. The exact role of iron in the development of osteoporosis in these disorders is not established. To define the effect of iron excess in bone, we generated an iron-overloaded mouse by...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-12-260083
更新日期：2010-10-07 00:00:00
abstract：:Using Philadelphia chromosome-positive (Ph+) chronic myelogenous leukemia (CML) as a model, our aim has been to develop a molecular cytogenetic method of high resolution analysis for monitoring the frequency of cells with nonrandom chromosome rearrangements in the bone marrow of patients receiving treatment for hemato...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-09-15 00:00:00
abstract：:Functional studies on native human dendritic cells (DCs) are hampered by technical difficulties in preparing fresh DCs. Recently, with the help of the monoclonal antibody M-DC8, we succeeded in isolating a major subpopulation of human blood DCs by a one-step immunomagnetic separation procedure. These cells strongly ex...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2002-08-15 00:00:00
abstract：:The periprocedural management of patients receiving long-term oral anticoagulant therapy remains a common but difficult clinical problem, with a lack of high-quality evidence to inform best practices. It is a patient's thromboembolic risk that drives the need for an aggressive periprocedural strategy, including the us...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2012-06-415943
更新日期：2012-10-11 00:00:00
abstract：:Idiopathic pneumonia syndrome (IPS) is a relatively common, frequently fatal clinical entity, characterized by noninfectious acute lung inflammation following allogeneic stem cell transplantation (SCT), the mechanisms of which are unclear. In this study, we demonstrate that immune suppression with cyclosporin after SC...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2014-07-590232
更新日期：2015-04-09 00:00:00
abstract：:Acquisition of mature T cell function and the T cell antigen receptor repertoire occur in the thymus. In an effort to delineate the cascade of events leading to T cell maturation, we analyzed a series of clonal human precursor T cell neoplasms representing early, middle, and late stages of intrathymic differentiation....
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:Hyaluronan-binding function of the CD44 molecule has not been so far detected in myeloid cells. To study pure populations of primitive myeloid cells, we investigated the hyaluronan-binding function of the CD44 molecule from three myeloid cell lines: KG1a, KG1, and HL60. Both KG1a and KG1 cells express the CD34 antigen...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Recurrence of the underlying malignancy remains a major cause of treatment failure after autologous bone marrow transplantation (BMT) for patients with lymphoma. In this regard, we have developed an immunotherapeutic approach designed to induce resistance against residual tumor cells persisting after BMT. Previous stu...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Commercial porcine factor VIII concentrate (Hyate:C) is effective in treatment of patients with hemophilia A who have circulating antibodies to factor VIII. The molecular forms of factor VIII in the concentrate were identified and evaluated in light of the known properties of porcine and human factor VIII. The factor ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Glanzmann thrombasthenia is an autosomal recessive disorder of the platelet glycoproteins (GP) IIb and IIIa. These glycoproteins normally serve as receptors for other adhesive glycoproteins, including fibrinogen, von Willebrand factor, and fibronectin. Most patients affected by Glanzmann thrombasthenia have low levels...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:JMH is a high-frequency human erythrocyte blood group antigen. Previous work has shown that JMH is absent from complement-sensitive erythrocytes of patients with paroxysmal nocturnal hemoglobinuria (PNH); such cells have a broad defect in expression of phosphatidylinositol (PI)-linked proteins. Using both human JMH an...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-03-15 00:00:00
abstract：:Red blood cells deliver O(2) from the lungs to every cell in the human body. Reduced tissue oxygenation triggers increased production of erythropoietin by hypoxia-inducible factor 1 (HIF-1), which is a transcriptional activator composed of an O(2)-regulated alpha subunit and a constitutively expressed beta subunit. Hy...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2009-05-189985
更新日期：2009-09-03 00:00:00
abstract：:The transforming growth factor-beta (TGF-beta) family of cytokines regulates vascular development and inflammatory responses. We have recently shown that exposure of human umbilical vein endothelial cells (HUVECs) to hypoxia (1% O(2)) increases gene expression and bioactivation of TGF-beta2 and induces its downstream ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-02-0629
更新日期：2003-03-15 00:00:00
abstract：:The authors have identified a 12-residue carboxyl-terminal extension of Lys-Ser-Pro-Met-Arg-Arg-Phe-Leu-Leu-Phe-Cys-Met in a dysfibrinogen derived from a woman heterozygotic for this abnormality and associated with severe bleeding. This extension is due to a T-to-A mutation that creates AAG encoding Lys at the stop (T...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:The role of platelets in hemostasis and thrombosis is clearly established; however, the mechanisms by which platelets mediate inflammatory and immune pathways are less well understood. Platelets interact and modulate the function of blood and vascular cells by releasing bioactive molecules. Although the platelet is an...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-12-396440
更新日期：2012-06-28 00:00:00
abstract：:Twenty-two molecular diagnostic laboratories from 14 countries participated in a consortium study to estimate the impact of Factor VIII gene inversions in severe hemophilia A. A total of 2,093 patients with severe hemophilia A were studied; of those, 740 (35%) had a type 1 (distal) factor VIII inversion, and 140 (7%) ...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：
更新日期：1995-09-15 00:00:00
abstract：:Self-renewal is a feature of cancer and can be assessed by cell transplantation into immune-compromised or immune-matched animals. However, studies in zebrafish have been severely limited by lack of these reagents. Here, Myc-induced T-cell acute lymphoblastic leukemias (T-ALLs) have been made in syngeneic, clonal zebr...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-10-246488
更新日期：2010-04-22 00:00:00
abstract：:High concentrations of bovine factor VIII cause clumping of platelets into a few very large aggregates. This response is termed superaggregation. It is distinct from factor-VIII-induced agglutination but is also independent of both extracellular calcium ions and platelet energy metabolism. Neither agglutinating lectin...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:The BCR-ABL1 fusion kinase is frequently associated with chronic myeloid leukemia and B-cell acute lymphoblastic leukemia but is rare in T-cell acute lymphoblastic leukemia (T-ALL). We recently identified NUP214-ABL1 as a variant ABL1 fusion gene in 6% of T-ALL patients. Here we describe the identification of another ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-12-4897
更新日期：2005-06-15 00:00:00
abstract：:The rapid transformation of human blood platelets from inert discoid cells to spheroechinocytes that is induced by adenosine diphosphate (ADP) has been followed by right-angle light scattering intensity measurements using a laser light source and a sensitive photomultiplier. Two steps have been observed, and their rat...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Natural-killer (NK)-cell dysfunction and IFN-gamma deficiencies have been associated with increased incidence of both malignancy and infection. The immunologic basis of NK-cell defects in cancer-bearing hosts has not been extensively studied. Here, we demonstrate that multiple lineages of tumors, including thymoma, br...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-11-4535
更新日期：2006-07-01 00:00:00
abstract：:Hypomethylating agents (HMAs) improve survival in patients with higher-risk myelodysplastic syndromes (MDS) but are less well-studied in lower-risk disease. We compared the safety and efficacy of low-dose decitabine vs low-dose azacitidine in this group of patients. Adults with low- or intermediate 1-risk MDS or MDS/m...
journal_title：Blood
pub_type： 杂志文章,随机对照试验
doi：10.1182/blood-2017-06-788497
更新日期：2017-09-28 00:00:00
abstract：:The combination of cyclophosphamide, vincristine, and prednisone (CVP) is one of several standard treatment options for advanced follicular lymphoma. This, like similar chemotherapeutic regimens, induces response rates of 60% to 80%, with a median response duration of under 2 years. Rituximab, a chimeric monoclonal an...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1182/blood-2004-08-3175
更新日期：2005-02-15 00:00:00
abstract：:We evaluated a treatment strategy targeting both lymphoma cells (by rituximab) and the microenvironment (by thalidomide) in 16 patients with relapsed/refractory mantle cell lymphoma (MCL). Rituximab was administered at 375 mg/m(2) for 4 weekly doses concomitantly with thalidomide (200 mg daily, with a dose increment t...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2004-03-1091
更新日期：2004-10-15 00:00:00
abstract：:Fibronectin is an adhesive protein that can promote phagocytosis and endothelial cell adhesion. Plasma fibronectin declines following burn in animals and patients, potentially due to its complexing with circulating collagenous debris as well as its rapid binding to sites of tissue injury. Such depletion of fibronectin...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Ristocetin and vancomycin are structurally similar glycopeptide antibiotics. Both vancomycin and ristocetin in high concentrations (3.0 mg/ml) cause the precipitation of fibrinogen, plasminogen, and IgG from platelet-poor plasma (PPP). In contrast to ristocetin, vanomycin (0.5-1.5 mg/ml) does not agglutinate platelets...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Multiple observations support the existence of developmental differences in megakaryocytopoiesis. We have previously shown that neonatal megakaryocyte (MK) progenitors are hyperproliferative and give rise to MKs smaller and of lower ploidy than adult MKs. Based on these characteristics, neonatal MKs have been consider...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-07-293092
更新日期：2011-04-14 00:00:00
