Abstract：
:Colony-forming capacity of human melanoma cells was investigated in five established cell lines and isolated cells from seven metastatic or recurrent tumors. A single-cell suspension was added to 0.3% agar medium, and colonies were observed over 7 to 21 days. With the five cell lines, colony formation occurred in all cases and was usually accelerated by 2-mercaptoethanol, but there was wide variation in cloning efficiency between the cell lines (5 to 80%), in the appearance of the colonies, and in their recloning capacity. In those cell lines with low cloning efficiency, some colonies grew attached to the culture dishes, and these had a high recloning capacity. In the melanotic cell lines, colonies originated from slightly or nonpigmented cells which became more pigmented during incubation. Stimulation of colony growth by 2-mercaptoethanol led to reduced pigmentation of colonies, whereas theophylline caused increased melanin production and decreased colony size. With freshly obtained tumor specimens, both the frequency of colonies (0.01 to 0.26%) and the growth rate were much reduced compared to the cell lines. Both could be increased by addition of 2-mercaptoethanol to the medium or by separation of the tumor cell suspension on a density gradient before culture.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Asano S,Riglar Csubject
Has Abstractpub_date
1981-03-01 00:00:00pages
1199-204issue
3eissn
0008-5472issn
1538-7445journal_volume
41pub_type
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