Abstract：
:Loss of heterozygosity (LOH) and replication error (RER) are important phenomena in tumor development, with diagnostic and prognostic relevance. Therefore, screening for LOH and RER is a desirable first step in the molecular analysis of tumors. We used semiautomated procedures based on multicolor fluorescently labeled microsatellite markers and an automated sequencer for PCR amplification, electrophoresis of PCR products, and allele detection with a set of 16 microsatellites in 56 colorectal tumors. We improved existing software for computer-assisted assessment of LOH and RER. A comparison of these results with those of a conventional, radioactive technique and visual interpretation shows a high degree of correlation between the two methods. The detection rates of LOH and RER are similar to those reported previously. The main advantages of the semiautomated fluorescence-based typing are in the objective, observer-unrelated, easy, and rapid computer-based scoring, and the resulting quantitative assessment of RER.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Canzian F,Salovaara R,Hemminki A,Kristo P,Chadwick RB,Aaltonen LA,de la Chapelle Asubject
Has Abstractpub_date
1996-07-15 00:00:00pages
3331-7issue
14eissn
0008-5472issn
1538-7445journal_volume
56pub_type
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