Functional Characterization and Partial Purification of the Ubiquinol-Cytochrome c Oxidoreductase from Higher Plant Mitochondria (Helianthus tuberosus).

Abstract:

:The functional and thermodynamic characteristics of the ubiquinolcytochrome (Cyt) c oxidoreductase in a Cyt b/c(1)-enriched fraction (defined S-1) isolated from Jerusalem artichoke mitochondria (JAM) (Helianthus tuberosus), have been analyzed. Fraction S-1, obtained through deoxycholate-KCl fractionation procedure, contained one Cyt of c type (formally c(1) with Em(7.0) of +240 millivolts), two b type Cyt with Em(7.0) values of +100 and -25 millivolts, ferredoxin-like centers presumably linked to succinic- and NADH-dehydrogenases, and a Rieske-type iron sulfur center (g(y) = 1.89). The ubiquinol-dependent Cyt c reduction by fraction S-1 showed sensitivity to antimycin A, myxothiazol, and n-2-hepthyl-1-hydroxyquinoline N-oxide with I(50) of 12 nanomolar, 30 nanomolar, and 0.1 micromolar, respectively. Oxidation-induced extra b type reduction, a widespread phenomenon of bacterial and mitochondrial respiratory systems, has also been observed in both intact mitochondria and S-1 fraction. The data seem to blur previous experiments in which both spectral and functional differences between higher plant and mammalian mitochondria have been underlined.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Esposti MD,Flamini E,Zannoni D

doi

10.1104/pp.77.3.758

subject

Has Abstract

pub_date

1985-03-01 00:00:00

pages

758-64

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

77

pub_type

杂志文章
  • Synthesis and apparent turnover of Acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.

    abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.879

    authors: Matsushita K,Uritani I

    更新日期:1977-05-01 00:00:00

  • CML42-mediated calcium signaling coordinates responses to Spodoptera herbivory and abiotic stresses in Arabidopsis.

    abstract::In the interaction between Arabidopsis (Arabidopsis thaliana) and the generalist herbivorous insect Spodoptera littoralis, little is known about early events in defense signaling and their link to downstream phytohormone pathways. S. littoralis oral secretions induced both Ca²⁺ and phytohormone elevation in Arabidopsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.198150

    authors: Vadassery J,Reichelt M,Hause B,Gershenzon J,Boland W,Mithöfer A

    更新日期:2012-07-01 00:00:00

  • Indole-3-acetic acid controls cambial growth in scots pine by positional signaling

    abstract::The vascular cambium produces secondary xylem and phloem in plants and is responsible for wood formation in forest trees. In this study we used a microscale mass-spectrometry technique coupled with cryosectioning to visualize the radial concentration gradient of endogenous indole-3-acetic acid (IAA) across the cambial...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.113

    authors: Uggla C,Mellerowicz EJ,Sundberg B

    更新日期:1998-05-01 00:00:00

  • Osmosensitive changes of carbohydrate metabolism in response to cellulose biosynthesis inhibition.

    abstract::Cellulose is the most abundant biopolymer in the world, the main load-bearing element in plant cell walls, and represents a major sink for carbon fixed during photosynthesis. Previous work has shown that photosynthetic activity is partially regulated by carbohydrate sinks. However, the coordination of cellulose biosyn...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.195198

    authors: Wormit A,Butt SM,Chairam I,McKenna JF,Nunes-Nesi A,Kjaer L,O'Donnelly K,Fernie AR,Woscholski R,Barter MC,Hamann T

    更新日期:2012-05-01 00:00:00

  • Variability in Proline-Accumulating Ability of Barley (Hordeum vulgare L.) Cultivars Induced by Vapor Pressure Deficit.

    abstract::This work was undertaken in an effort to reconcile the conflicting proline-accumulating responses of the barley (Hordeum vulgare L.) cultivars, Excelsior and Proctor, reported by Singh et al. (1972) and Hanson et al. (1976). It deals with the effects of different vapor pressure deficits (VPD) during growth and subsequ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.716

    authors: Naidu BP,Aspinall D,Paleg LG

    更新日期:1992-02-01 00:00:00

  • Altered gene expression in three plant species in response to treatment with Nep1, a fungal protein that causes necrosis.

    abstract::Nep1 is an extracellular fungal protein that causes necrosis when applied to many dicotyledonous plants, including invasive weed species. Using transmission electron microscopy, it was determined that application of Nep1 (1.0 micro g mL(-)(1), 0.1% [v/v] Silwet-L77) to Arabidopsis and two invasive weed species, spotte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.019836

    authors: Keates SE,Kostman TA,Anderson JD,Bailey BA

    更新日期:2003-07-01 00:00:00

  • Cytokinin-controlled Indoleacetic Acid Oxidase Isoenzymes in Tobacco Callus Cultures.

    abstract::Indoleacetic acid oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) was resolved into seven anionic isoenzymes by polyacrylamide gel disc electrophoresis. Different concentrations of kinetin and zeatin in the presence of indoleacetic acid affected the level of this enzyme, particularly two ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.2.181

    authors: Lee TT

    更新日期:1971-02-01 00:00:00

  • Chloroplast thylakoid protein phosphatase is a membrane surface-associated activity.

    abstract::Chloroplast thylakoid protein phosphatase activity was measured using (32)P-labeled histone as an exogenous substrate and an assay of the (32)Pi released involving formation of a phosphomolybdate complex and organic extraction. The activity was liberated from wheat (Triticum aestivum) thylakoids by washing the membran...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.238

    authors: Sun G,Bailey D,Jones MW,Markwell J

    更新日期:1989-01-01 00:00:00

  • Characterization of photorespiration in intact leaves using carbon dioxide labeling.

    abstract::The (13)C nuclear magnetic resonance spectra of sucrose extracted from intact soybean and corn leaves labeled with (13)CO(2) for 5 to 180 minutes have been obtained at 22.6 megahertz. The spectra provide a direct determination of the relative concentrations of (12)C-(13)C and (13)C-(13)C pairs of carbons in the labele...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.254

    authors: Schaefer J,Kier LD,Stejskal EO

    更新日期:1980-02-01 00:00:00

  • Changes to the Stoichiometry of Glycine Decarboxylase Subunits during Wheat (Triticum aestivum L.) and Pea (Pisum sativum L.) Leaf Development.

    abstract::Changes in the levels of the four subunits of the mitochondrial enzyme glycine decarboxylase (EC 2.1.2.10) have been investigated during development in the 8 day old primary leaf of wheat (Triticum aestivum L.). Proteins were extracted from wheat leaf sections between the basal meristem and 8.5 centimeters. The indivi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.952

    authors: Rogers WJ,Jordan BR,Rawsthorne S,Tobin AK

    更新日期:1991-07-01 00:00:00

  • Arabidopsis HMA2, a divalent heavy metal-transporting P(IB)-type ATPase, is involved in cytoplasmic Zn2+ homeostasis.

    abstract::PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.046292

    authors: Eren E,Argüello JM

    更新日期:2004-11-01 00:00:00

  • Flowering responses to altered expression of phytochrome in mutants and transgenic lines of Arabidopsis thaliana (L.) Heynh.

    abstract::The long-day plant Arabidopsis thaliana (L.) Heynh. flowers early in response to brief end-of-day (EOD) exposures to far-red light (FR) following a fluorescent short day of 8 h. FR promotion of flowering was nullified by subsequent brief red light (R) EOD exposure, indicating phytochrome involvement. The EOD response ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1495

    authors: Bagnall DJ,King RW,Whitelam GC,Boylan MT,Wagner D,Quail PH

    更新日期:1995-08-01 00:00:00

  • Phytochrome B and at Least One Other Phytochrome Mediate the Accelerated Flowering Response of Arabidopsis thaliana L. to Low Red/Far-Red Ratio.

    abstract::We have investigated the involvement of phytochrome B in the early-flowering response of Arabidopsis thaliana L. seedlings to low red:far-red (R/FR) ratio light conditions. The phytochrome B-deficient hy3 (phyB) mutant is early flowering, and in this regard it resembles the shade-avoidance phenotype of its isogenic wi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.4.1311

    authors: Halliday KJ,Koornneef M,Whitelam GC

    更新日期:1994-04-01 00:00:00

  • Selective and Reversible Inhibition of Active CO(2) Transport by Hydrogen Sulfide in a Cyanobacterium.

    abstract::The active transport of CO(2) in the cyanobacterium Synechococcus UTEX 625 was inhibited by H(2)S. Treatment of the cells with up to 150 micromolar H(2)S + HS(-) at pH 8.0 had little effect on Na(+)-dependent HCO(3) (-) transport or photosynthetic O(2) evolution, but CO(2) transport was inhibited by more than 90%. CO(...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.387

    authors: Espie GS,Miller AG,Canvin DT

    更新日期:1989-09-01 00:00:00

  • Osmotic adjustment by intact isolated chloroplasts in response to osmotic stress and its effect on photosynthesis and chloroplast volume.

    abstract::Spinach leaf chloroplasts isolated in isotonic media (330 millimolar sorbitol, -1.0 megapascals osmotic potential) had optimum rates of photosynthesis when assayed at -1.0 megapascals. When chloroplasts were isolated in hypertonic media (720 millimolar sorbitol, -2.0 megapascals osmotic potential) the optimum osmotic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.4.996

    authors: Robinson SP

    更新日期:1985-12-01 00:00:00

  • Nickel in Plants: II. Distribution and Chemical Form in Soybean Plants.

    abstract::The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.566

    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1978-10-01 00:00:00

  • A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.

    abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01527

    authors: Wang X,Guan Y,Zhang D,Dong X,Tian L,Qu LQ

    更新日期:2017-02-01 00:00:00

  • Osmotic stress tolerance of transgenic tobacco expressing a gene encoding a membrane-located receptor-like protein from tobacco plants.

    abstract::Tobacco (Nicotiana tabacum) genes regulated during the early stage of responses to wounding were screened by a modified fluorescence differential display method. Among 28 genes initially identified, a particular clone designated NtC7 was subjected to further analysis. Its transcripts were found to accumulate rapidly a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.011007

    authors: Tamura T,Hara K,Yamaguchi Y,Koizumi N,Sano H

    更新日期:2003-02-01 00:00:00

  • Kinetic Properties of Phosphoenolpyruvate Carboxylase in Peperomia camptotricha.

    abstract::Kinetic characteristics of phosphoenolpyruvate carboxylase (PEPC) from the epiphytic C(3) or C(4): CAM intermediate plant, Peperomia camptotricha, were investigated. Few day versus night differences in V(max),K(m(PEP))), or malate inhibition were observed, even in extracts from water-stressed plants which characterist...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.1050

    authors: Sipes DL,Ting IP

    更新日期:1989-11-01 00:00:00

  • The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes of sporophytes.

    abstract::Excised juvenile leaves of Microgramma vacciniifolia (Polypodiaceae) develop sporophytic regenerants when grown on mineral agar with sucrose. The ratio of sporophytes to gametophytes produced from the leaf tissue increases with higher percentages of sucrose such that at 4% sucrose, the induction of aposporous gametoph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.3.390

    authors: Hirsch AM

    更新日期:1975-09-01 00:00:00

  • Purification and Developmental Analysis of an Extracellular Proteinase from Young Leaves of Soybean.

    abstract::A proteinase present in intercellular wash fluids from leaves of Glycine max has been purified 600-fold to electrophoretic homogeneity. The native protein is monomeric with a molecular mass of 60 kD, as estimated by denaturing gel electrophoresis, and has an isoelectric point of 7.7. The enzyme has a pH optimum of 9.5...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.3.969

    authors: Huangpu J,Graham JS

    更新日期:1995-07-01 00:00:00

  • Synthesis of abscisic Acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain.

    abstract::Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set of heat-stable proteins. Two of these proteins were identified as de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.662

    authors: Ried JL,Walker-Simmons MK

    更新日期:1990-06-01 00:00:00

  • Characterization of a meta-Fluorotyrosine-Tolerant Cell Culture of Eschscholtzia californica Cham.

    abstract::A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1299

    authors: Berlin J,van Huystee RB,Witte L,Bender J,Jäger HJ,Wray V

    更新日期:1989-04-01 00:00:00

  • Triparanol Inhibition of Sterol Biosynthesis in Chlorella ellipsoidea.

    abstract::The sterol composition of C. ellipsoidea was markedly changed when this alga was grown in the presence of 1 mug/g triparanol. Triparanol appears to inhibit the removal of 14alpha-methyl group, the second alkylation at C-24, Delta(7)-reductase, and Delta(8) --> Delta(7)-isomerase. The effect of triparanol in Chlorella ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.3.246

    authors: Chan JT,Patterson GW

    更新日期:1973-09-01 00:00:00

  • The structure of chloroplast DNA molecules and the effects of light on the amount of chloroplast DNA during development in Medicago truncatula.

    abstract::We used pulsed-field gel electrophoresis and restriction fragment mapping to analyze the structure of Medicago truncatula chloroplast DNA (cpDNA). We find most cpDNA in genome-sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at defined sites rather than at random sites as ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.112946

    authors: Shaver JM,Oldenburg DJ,Bendich AJ

    更新日期:2008-03-01 00:00:00

  • Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Phosphomonoesterase in Dunaliella salina Membranes.

    abstract::In comparison with other cell organelles, the Dunaliella salina plasma membrane was found to be highly enriched in phospholipase C activity toward exogenous [(3)H]phosphatidylinositol 4,5-bisphosphate (PIP(2)). Based on release of [(3)H]inositol phosphates, the plasma membrane exhibited a PIP(2)-phospholipase C activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1115

    authors: Einspahr KJ,Peeler TC,Thompson GA

    更新日期:1989-07-01 00:00:00

  • Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures.

    abstract::Although a number of plant natural products are derived from benzoic acid, the biosynthesis of this structurally simple precursor is poorly understood. Hypericum calycinum cell cultures accumulate a benzoic acid-derived xanthone phytoalexin, hyperxanthone E, in response to elicitor treatment. Using a subtracted comple...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.204180

    authors: Gaid MM,Sircar D,Müller A,Beuerle T,Liu B,Ernst L,Hänsch R,Beerhues L

    更新日期:2012-11-01 00:00:00

  • Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit.

    abstract::We have previously described the construction and expression of a chimeric gene that allows developmentally regulated expression of tomato (Lycopersicon esculentum) polygalacturonase in ripening-impaired, mutant (rin) tomato fruit (JJ Giovannoni, D DellaPenna, AB Bennett, RL Fischer [1989] The Plant Cell 1: 53-63). We...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.4.1882

    authors: Dellapenna D,Lashbrook CC,Toenjes K,Giovannoni JJ,Fischer RL,Bennett AB

    更新日期:1990-12-01 00:00:00

  • Abscisic Acid Content, Transpiration, and Stomatal Conductance As Related to Leaf Age in Plants of Xanthium strumarium L.

    abstract::Among the four uppermost leaves of greenhouse-grown plants of Xanthium strumarium L. the content of abscisic acid per unit fresh or dry weight was highest in the youngest leaf and decreased gradually with increasing age of the leaves. Expressed per leaf, the second youngest leaf was richest in ABA; the amount of ABA p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.2.169

    authors: Raschke K,Zeevaart JA

    更新日期:1976-08-01 00:00:00

  • The Arabidopsis Paf1c complex component CDC73 participates in the modification of FLOWERING LOCUS C chromatin.

    abstract::FLOWERING LOCUS C (FLC) is a key repressor of flowering in Arabidopsis (Arabidopsis thaliana) and is regulated, both positively and negatively, by posttranslational histone modifications. For example, vernalization (the promotion of flowering by cold temperatures) epigenetically silences FLC expression through repress...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158386

    authors: Yu X,Michaels SD

    更新日期:2010-07-01 00:00:00