Abstract:
BACKGROUND:The purpose of the present paper was to investigate dendritic cell (DC) and T-cell functions in patients with chronic hepatitis B (CHB) and determine whether therapeutic DC vaccines could restore T-cell function in those patients in vitro. METHODS:Twelve patients with CHB and 10 normal control subjects with positivity for antibodies to hepatitis B surface and core antigens (anti-HBs and anti-HBc positivity) were enrolled in the present study. Phenotype analysis and allogeneic mixed lymphocyte reaction assay of DC from CHB patients and normal controls were made in the absence or presence of a cocktail of cytokines: interleukin-1beta (IL-1beta), prostaglandin E(2) (PGE(2)), IL-6 and tumor necrosis factor-alpha (TNF-alpha). Autologous T-cell proliferation assays and the enzyme-linked immunospot (ELISPOT) method for detecting interferon-gamma (IFN-gamma)-producing CD8(+) T cells were used to evaluate the efficacy of DC loaded in vitro with HBsAg or HBcAg. RESULTS:The DC from CHB patients had a lower expression of costimulatory molecules CD80, CD86 and impaired allogeneic mixed lymphocyte reaction capacity compared to those from normal controls. However, the impaired DC function could be restored partially by cytokine cocktail supplemented in vitro. Mature DC loaded with HBsAg or HBcAg showed a greater capacity for autologous T-cell proliferation and antigen-specific IFN-gamma production than immature DC. Moreover, as a DC -loading antigen, HBcAg was more immunogenic than HBsAg. CONCLUSIONS:The impaired function of DC in patients with CHB may be restored by supplementation in vitro with a cocktail of cytokines, and therapeutic DC vaccines might be effective to treat CHB infection in humans.
journal_name
J Gastroenterol Hepatoljournal_title
Journal of gastroenterology and hepatologyauthors
Duan XZ,He HX,Zhuang Hdoi
10.1111/j.1440-1746.2006.04262.xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
970-6issue
6eissn
0815-9319issn
1440-1746pii
JGHjournal_volume
21pub_type
杂志文章abstract:BACKGROUND AND AIM:A high yield of biopsy is mandatory to perform molecular genetic research with endoscopically obtained gastric cancer tissues. We evaluated whether probe-based confocal laser endomicroscopy (pCLE) can increase the yield of endoscopic biopsy for gastric cancer compared with white light endoscopy (WLE)...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章,随机对照试验
doi:10.1111/jgh.14471
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND AND AIM:It has been reported that immunological factors, such as the T-helper cell (Th)1/Th2 ratio, play a part in the mechanisms for the antihepatitis C virus (HCV) effect of the interferon (IFN) alpha-2b plus ribavirin combination therapy. By using flow cytometry, we examined this ratio during a 24-week co...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2008.05320.x
更新日期:2008-08-01 00:00:00
abstract::The expression of thrombomodulin on sinusoidal endothelial cells was studied using the immuno-peroxidase method and both light and electron microscopy in patients with chronic viral hepatitis (type B: four cases; type C: 28 cases). Thrombomodulin was found on sinusoidal endothelial cells, especially on the surface in ...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.1995.tb01805.x
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND AND AIM:As bacterial resistance to clarithromycin limits the efficacy of clarithromycin-based regimens for Helicobacter pylori infection, attention has turned to quinolone-based rescue therapies. Resistance of H. pylori to both clarithromycin and quinolone can be predicted by genetic testing. Here, we used t...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2011.06815.x
更新日期:2011-09-01 00:00:00
abstract::Spontaneous regression of hepatocellular carcinoma (HCC) is a rare phenomenon. This case of a 65-year-old Korean man with HCC and metastatic frontal bone mass that regressed after radiotherapy for frontal bone mass without any other therapeutic modalities is described. The clinical diagnosis of HCC was made because of...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2005.03243.x
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND AND AIMS:Regional variations in inflammatory bowel disease (IBD) rates have been observed. Limited epidemiological data are available from Australasia. IBD prevalence rates have never been assessed in an Australian population-based setting. In addition, there are few historical IBD incidence data to allow as...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.13050
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND AND AIM:Hepatitis B core-related antigen (HBcrAg) has been shown to correlate with various viral markers in chronic hepatitis B, but its role in defining natural history is not well studied. We aimed to investigate the use of HBcrAg to define different phases of chronic hepatitis B. METHODS:Stored residual ...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.15058
更新日期:2020-04-15 00:00:00
abstract:BACKGROUND:Indoleamine 2,3-dioxygenase (IDO) is a tryptophan catabolic enzyme. Recent studies have focused on the immunoregulatory role of IDO in mononuclear cells. The role of IDO in hepatocellular carcinoma (HCC) cell lines and HCC patients was examined. METHODS:The expression of IDO mRNA in peripheral blood mononuc...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2003.03259.x
更新日期:2004-03-01 00:00:00
abstract::Autoimmune hepatitis (AIH) is an inflammatory liver disease with diverse clinical spectrum, which predominantly affects females. This review provides detailed comparisons of epidemiology, genetic predispositions, clinical features, risk factors of hepatocellular carcinoma, and mortality in AIH patients between eastern...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章,评审
doi:10.1111/jgh.12952
更新日期:2015-08-01 00:00:00
abstract::Classical Kaposi's sarcoma (KS) is an indolent neoplasm involving mucocutaneous sites predominantly in elderly Mediterranean or Jewish persons. Whilst gastrointestinal involvement is common, it is usually asymptomatic. This case report presents a case of massive gastrointestinal haemorrhage in a patient with stable cu...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.1996.tb01391.x
更新日期:1996-04-01 00:00:00
abstract:BACKGROUND AND AIM:The aim of the present study was to compare the therapeutic efficacy of three different regimens in childhood chronic hepatitis B (CHB) infection. METHODS:A total of 182 children with CHB infection were prospectively allocated to three random groups. Sixty-two patients in the first group received hi...
journal_title:Journal of gastroenterology and hepatology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1440-1746.2004.03209.x
更新日期:2004-02-01 00:00:00
abstract::Hepatitis C virus infection is a global health problem; however, the interaction between pregnancy and chronic hepatitis C remains controversial. A Taiwanese woman with chronic hepatitis C had an uncomplicated pregnancy and gave birth to a female baby through spontaneous vaginal delivery. The serum levels of alanine a...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1046/j.1440-1746.2001.02502.x
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND AND AIM:Whether an antiviral interferon (IFN)-based therapy (IBT) after curative treatment of hepatocellular carcinoma (HCC) improves the prognosis in patients with hepatitis C virus (HCV)-related HCC remains to be elucidated. METHODS:A total of 178 patients within the Milan criteria underwent curative trea...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.12925
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND AND AIM:Non-invasive markers are essential to assess the progression of chronic liver diseases to fibrosis/ cirrhosis and the effectiveness of therapeutic strategies. The aim of this study was to evaluate the ability of non-invasive markers to identify significant fibrosis, severe fibrosis, and cirrhosis in ...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.13407
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND AND AIM:Monotherapy has been proven insufficient in achieving sustained control of chronic hepatitis B. We aimed to assess the efficacy of combined sequential administration of lamivudine and pegylated interferon alfa-2b in patients with hepatitis Be antigen (HBeAg)-negative chronic hepatitis B. METHODS:Eig...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2007.05103.x
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND AND AIM:Although several molecular biomarkers for esophageal adenocarcinoma (EAC) have been shown to be useful disease indicators, none has been established as a reliable indicator for risk of EAC or have progressed to routine use. The aim was to identify biomarkers of high risk for EAC in patients with Barr...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.13055
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND AND AIMS:This study was aimed to find clinical predictors for developing delirium tremens (DT) in alcohol dependence. METHODS:This cohort study was retrospectively carried out among patients who were diagnosed as having alcohol dependence between January 2001 and July 2004. Fifteen parameters were compared ...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2005.03932.x
更新日期:2005-12-01 00:00:00
abstract::Antipyrine metabolism is widely used as an index of the drug-metabolizing reserve of the liver. It is well known that metabolism of this drug is impaired in subjects with acute hepatitis or cirrhosis, but conflicting data have been reported regarding patients with chronic postinfectious hepatitis or liver cancer. We s...
journal_title:Journal of gastroenterology and hepatology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1440-1746.1998.tb00668.x
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND AND AIM:Hepatorenal syndrome (HRS) occurs in patients with advanced liver cirrhosis and has a poor outcome. The aim of the present study was to investigate which patients with HRS are likely to benefit from hemodialysis. METHODS:Data were collected prospectively from 30 patients with Child-Pugh C liver cirr...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2004.03471.x
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND AND AIMS:The (13)C-urea breath test ((13)C-UBT) is a reliable non-invasive method of diagnosing Helicobacter pylori infection in adults and children. However, only a few validation studies have been performed on the (13)C-UBT in very young children. The purpose of the present study was to evaluate the diagno...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.2004.03541.x
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND AND AIM:Physicians use different scales and questionnaires to assess the severity of clinical symptoms in patients with functional gastrointestinal disorders. The current study aimed to validate the "7 × 7" questionnaire for assessment of severity of the symptoms as a tool for the efficacy of treatment of fu...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.14546
更新日期:2019-06-01 00:00:00
abstract:AIM:The clinical presentation of four children and adolescents (two males and two females with a mean age of 12.4 years; range 9-16 years) with colorectal spirochetosis is discussed. RESULTS:Symptoms included persistent diarrhea (n = 2), rectal bleeding (n = 1) and abdominal pain (n = 2). In all patients, colorectal s...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1046/j.1440-1746.2001.t01-1-02543.x
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND AND AIM:Renal abnormalities can occur at any time point during the course of Wilson disease (WD). We aimed to fill a literature gap in this respect by studying urinary abnormalities in children and adolescents with WD. METHODS:This study included 60 children with WD presenting to the Pediatric Hepatology Un...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.14653
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Data on anorectal physiology in patients with disordered thyroid metabolism are lacking. This prospective study was performed to evaluate anorectal physiology in patients with either hyperthyroidism and diarrhoea, or hypothyroidism and constipation in order to assess slow transit in hypothyroid patients. ME...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1046/j.1440-1746.1999.01865.x
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Collagenous sprue (CS) is a rare form of enteropathy that had been reported to be associated with celiac disease (CD) and collagenous colitis (CC). The aim of our study was to compare the clinical features, treatments, and outcomes of CS, CD, and CC. METHODS:All patients with histologic diagnosis of CS, CD,...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/jgh.13592
更新日期:2017-01-01 00:00:00
abstract::Three clinicopathological phases of chronic hepatitis B virus (HBV) infection are identified. First, is immune tolerance of HBV. High levels of viraemia are associated with normal alanine aminotransferase (ALT) levels and minimal histological lesions. More than 30-40% of hepatocytes have the hepatitis B core antigen (...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章,评审
doi:10.1111/j.1440-1746.1997.tb00503.x
更新日期:1997-10-01 00:00:00
abstract::The three-dimensional ultrastructure of nuclei and cell organelles including rough-surfaced endoplasmic reticulum (RER), mitochondria, and cytoskeleton were studied in normal rat hepatocytes by the quick-freezing and deep-etching method. Peroxisomes and mitochondria were observed as spherical structures with granular ...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.1992.tb01025.x
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND AND AIM:There are few studies investigating the treatment of hepatitis C virus (HCV) infection in current and former drug users. With this in mind, we sought to evaluate the antiviral efficacy of interferon alpha-2b (IFN alpha-2b) or pegylated-interferon alpha-2b (PEG-IFN alpha-2b) and ribavirin (RBV) in inj...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1440-1746.2007.05032.x
更新日期:2007-09-01 00:00:00
abstract::The key issues that determine the decision between reusable versus disposable accessories are cost and functionality. In most health-care systems the availability and dissemination of endoscopic services relates directly to the resources (i.e. budget) of that system. Given the limitations of health-care budgets, acces...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章,评审
doi:10.1046/j.1440-1746.2000.02272.x
更新日期:2000-10-01 00:00:00
abstract::Sinusoidal endothelial cell damage is produced by activation of hepatic macrophages after endotoxin administration in rats pretreated with Cornyebacterium parvum or undergoing 70% hepatectomy. Such damage causes fibrin deposition in the hepatic sinusoids leading to massive hepatic necrosis. In the C. parvum model, cyt...
journal_title:Journal of gastroenterology and hepatology
pub_type: 杂志文章
doi:10.1111/j.1440-1746.1995.tb01795.x
更新日期:1995-01-01 00:00:00