Abstract：
:To investigate the relationship between cellular microelasticity and the structural features of cytoskeletons (CSKs), a microindentation test for apical cell membranes and observation of the spatio-distribution of actin CSKs of fibroblasts were performed by fluorescence and atomic force microscopy (FM/AFM). The indentation depths of apical cell membranes were measured from AFM force-indentation (f-i) curves under equal final loads and mapped two-dimensionally to show the relative distribution of local microelasticity on cell membranes. Intracellular spatial distribution of actin CSKs was visualized fluorescently by high Z-resolution cross-sectional observation of a cell on which indentation mapping analysis had been performed in advance. Structural features of stress fibers (SFs) were observed as three typical patterns of dense SF, sparse SF and sparser SF cell groups, which were quantitated using the degree of orientation in apical SFs (ASFs) that had been defined using two-dimensional Fourier analysis. In indentation depth maps, the upper nuclear region was markedly softer than the pseudopodium region. The mean indentation depth of the upper nuclear region decreased with increased SF density in whole cells and the degree of orientation of ASF, although the pseudopodium region did not exhibit such a trend. The apical membrane of adhered cells was found to tend to stiffen with the increase in both density and degree of orientation of SFs.
journal_name
Biomech Model Mechanobioljournal_title
Biomechanics and modeling in mechanobiologyauthors
Kidoaki S,Matsuda T,Yoshikawa Kdoi
10.1007/s10237-006-0048-8subject
Has Abstractpub_date
2006-11-01 00:00:00pages
263-72issue
4eissn
1617-7959issn
1617-7940journal_volume
5pub_type
杂志文章abstract：:Cell deformability and mechanical responses of living cells depend closely on the dynamic changes in the structural architecture of the cytoskeleton (CSK). To describe the dynamic reorganization and the heterogeneity of the prestressed multi-modular CSK, we developed a two-dimensional model for the CSK which was taken...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-006-0057-7
更新日期：2007-11-01 00:00:00
abstract：:The controlled cortical impact (CCI) model is widely used in many laboratories to study traumatic brain injury (TBI). Although external impact parameters during CCI tests could be clearly defined, little is known about the internal tissue-level mechanical responses of the rat brain. Furthermore, the external impact pa...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0212-z
更新日期：2010-12-01 00:00:00
abstract：:Plaque progression and vulnerability are influenced by many risk factors. Our goal is to find a simple method to combine multiple risk factors for better plaque development prediction. Intravascular ultrasound data at baseline and follow-up were acquired from nine patients, and fluid-structure interaction models were ...
journal_title：Biomechanics and modeling in mechanobiology
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更新日期：2019-10-01 00:00:00
abstract：:Both the clinically established diameter criterion and novel approaches of computational finite element (FE) analyses for rupture risk stratification of abdominal aortic aneurysms (AAA) are based on assumptions of population-averaged, uniform material properties for the AAA wall. The presence of inter-patient and intr...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-012-0436-1
更新日期：2013-08-01 00:00:00
abstract：:Bone fracture healing is a complex process in which angiogenesis or the development of a blood vessel network plays a crucial role. In this paper, a mathematical model is presented that simulates the biological aspects of fracture healing including the formation of individual blood vessels. The model consists of parti...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-010-0241-7
更新日期：2011-06-01 00:00:00
abstract：:Recently, physiological and biomechanical studies on animal models with metal implants filling full-thickness cartilage defects have resulted in good clinical outcomes. The knowledge of the time-dependent macroscopic behavior of cartilage surrounding the metal implant is essential for understanding the joint function ...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-011-0346-7
更新日期：2012-05-01 00:00:00
abstract：:Mechanical cues that trigger pathological remodeling in smooth muscle tissues remain largely unknown and are thought to be pivotal triggers for strain-induced remodeling. Thus, an understanding of the effects mechanical stimulation is important to elucidate underlying mechanisms of disease states and in the developmen...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-011-0298-y
更新日期：2012-01-01 00:00:00
abstract：:Long-term adaptation of soft tissues is realized through growth and remodeling (G&R). Mathematical models are powerful tools in testing hypotheses on G&R and supporting the design and interpretation of experiments. Most theoretical G&R studies concentrate on description of either growth or remodeling. Our model combin...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0204-z
更新日期：2010-12-01 00:00:00
abstract：:The mechanical behavior of most biological soft tissue is nonlinear viscoelastic rather than elastic. Many of the models previously proposed for soft tissue involve ad hoc systems of springs and dashpots or require measurement of time-dependent constitutive coefficient functions. The model proposed here is a system of...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-004-0055-6
更新日期：2005-03-01 00:00:00
abstract：:The primary capillary plexus in early yolk sacs is remodeled into matured vitelline vessels aligned in the direction of blood flow at the onset of cardiac contraction. We hypothesized that the influence of fluid shear stress on cellular behaviors may be an underlying mechanism by which some existing capillary channels...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-005-0001-2
更新日期：2005-12-01 00:00:00
abstract：:We propose a multiscale mechanobiological model of bone remodelling to investigate the site-specific evolution of bone volume fraction across the midshaft of a femur. The model includes hormonal regulation and biochemical coupling of bone cell populations, the influence of the microstructure on bone turnover rate, and...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-015-0705-x
更新日期：2016-02-01 00:00:00
abstract：:Digital models based on finite element (FE) analysis are widely used in orthopaedics to predict the stress or strain in the bone due to bone-implant interaction. The usability of the model depends strongly on the bone material description. The material model that is most commonly used is based on a constant Young's mo...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-016-0822-1
更新日期：2017-02-01 00:00:00
abstract：:Thrombosis is a major concern in blood-coated medical devices. Contact activation, which is the initial part of the coagulation cascade in device-related thrombosis, is not considered in current thrombus formation models. In the present study, pro-coagulant reactions including the contact activation system are coupled...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-017-0994-3
更新日期：2018-06-01 00:00:00
abstract：:Medical studies have consistently shown that the best defense against cancer is early detection. Due to this, many efforts have been made to develop methods of screening patient blood quickly and cheaply. These methods range from separation via differences in size, electrostatic potential, chemical potential, antibody...
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doi：10.1007/s10237-018-1093-9
更新日期：2019-04-01 00:00:00
abstract：:Disease alters tissue microstructure, which in turn affects the macroscopic mechanical properties of tissue. In elasticity imaging, the macroscopic response is measured and is used to infer the spatial distribution of the elastic constitutive parameters. When an empirical constitutive model is used, these parameters c...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-016-0831-0
更新日期：2017-04-01 00:00:00
abstract：:Finite element (FE) simulations of contractile responses of vascular muscular thin films (vMTFs) and endothelial cells resting on an array of microposts under stimulation of soluble factors were conducted in comparison with experimental measurements reported in the literature. Two types of constitutive models were emp...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-017-0886-6
更新日期：2017-08-01 00:00:00
abstract：:The calcaneus bone is formed of extensive trabecular bone and is therefore well suited to be used as an example of loaded bone to establish the ability of combining microfinite element (microFE) technique with high-resolution peripheral quantitative computed tomography (HR-pQCT) in determining its mechanical propertie...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-1051-6
更新日期：2018-12-01 00:00:00
abstract：:Adherent cells sense their mechanical environment, which, in turn, regulates their functions. During the past decade, a growing body of evidence has indicated that a deformable, solid-state intracellular structure known as the cytoskeleton (CSK) plays a major role in transmitting and distributing mechanical stresses w...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章,评审
doi：10.1007/s10237-002-0009-9
更新日期：2002-06-01 00:00:00
abstract：:The aim of the study was to establish a user-friendly approach for single fluorescence particle 3D localization and tracking with nanometre precision in a standard fluorescence microscope using a point spread function (PSF) approach, and to evaluate validity and precision for different analysis methods and optical con...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-013-0499-7
更新日期：2014-04-01 00:00:00
abstract：:In the present work, a constitutive model for articular cartilage is proposed in finite elasto-viscoplasticity. For simplification, articular cartilage is supposed to be a typical composite composed of a soft basis and a fiber assembly. The stress tensor and free energy function are hence accordingly divided into two ...
journal_title：Biomechanics and modeling in mechanobiology
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更新日期：2018-12-01 00:00:00
abstract：:Hemolysis is a major concern in blood-circulating devices, which arises due to hydrodynamic loading on red blood cells from ambient flow environment. Hemolysis estimation models have often been used to aid hemocompatibility design. The preponderance of hemolysis models was formulated on the basis of laminar flows. How...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-01108-y
更新日期：2019-06-01 00:00:00
abstract：:The paper describes the extension of a previously developed model of pressure-dependent contraction rate to the case of multiple lymphangions. Mechanical factors are key modulators of active lymphatic pumping. As part of the evolution of our lumped-parameter model to match experimental findings, we have designed an al...
journal_title：Biomechanics and modeling in mechanobiology
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更新日期：2018-10-01 00:00:00
abstract：:This study evaluated the ability of different in vitro mechanical loading tests to promote new mineral formation at bonded dentin interfaces. This research demonstrated a sequential transition in the dentin remineralizing procedure through the analysis of the mineral and matrix gradients. Mechanical loading in phospho...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-014-0573-9
更新日期：2014-11-01 00:00:00
abstract：:An efficient solver for large-scale linear [Formula: see text] simulations was extended for nonlinear material behavior. The material model included damage-based tissue degradation and fracture. The new framework was applied to 20 trabecular biopsies with a mesh resolution of [Formula: see text]. Suitable material par...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-019-01254-x
更新日期：2020-06-01 00:00:00
abstract：:The compliance of the proximal aortic wall is a major determinant of cardiac afterload. Aortic compliance is often estimated based on cross-sectional area changes over the pulse pressure, under the assumption of a negligible longitudinal stretch during the pulse. However, the proximal aorta is subjected to significant...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-020-01371-y
更新日期：2020-07-31 00:00:00
abstract：:Evidence suggests that mechanical load is related to structural destruction of disk annulus fibrosus (AF) either in adult disk degeneration or in child disk acute injury. Both biochemical and biomechanical properties are different between immature and mature disks. However, the effects of mechanical compression on imm...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-016-0823-0
更新日期：2017-04-01 00:00:00
abstract：:In myocardial infarction, muscle tissue of the heart is damaged as a result of ceased or severely impaired blood flow. Survivors have an increased risk of further complications, possibly leading to heart failure. Material properties play an important role in determining post-infarction outcome. Due to spatial variatio...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-018-1028-5
更新日期：2018-10-01 00:00:00
abstract：:This study presents a method for estimating the spatial variations in material properties of elastic membranes, such as biological tissue, which contain both inhomogeneous strain fields and inhomogeneous material properties. In order to validate the method, an inhomogeneous, isotropic rubber membrane was biaxially loa...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-002-0019-7
更新日期：2002-12-01 00:00:00
abstract：:Ventricular growth is widely considered to be an important feature in the adverse progression of heart diseases, whereas reverse ventricular growth (or reverse remodeling) is often considered to be a favorable response to clinical intervention. In recent years, a number of theoretical models have been proposed to mode...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-014-0598-0
更新日期：2015-04-01 00:00:00
abstract：:The diseases of human blood vessels are closely associated with local mechanical variations. A better understanding of the quantitative correlation in mechanical environment between the current mechano-biological studies and vascular physiological or pathological conditions in vivo is crucial for evaluating numerous e...
journal_title：Biomechanics and modeling in mechanobiology
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doi：10.1007/s10237-019-01226-1
更新日期：2020-04-01 00:00:00
