[Immunohistochemical analysis of alpha-SMA and GFAP expression in liver stellate cells].

Abstract:

BACKGROUND/AIM:Liver stellate cells play an important role in hepatic fibrosis, and its progression to cirrhosis. These cells show immunoreactivity with different monoclonal antibodies amongst which the commonest are alpha-smooth muscle actin (alpha-SMA) and glial fibrillary acid protein (GFAP). The aim of this study was to analyze stellate cell immunoreactivity for alpha-SMA and GFAP in tissue sections showing the signs of chronic viral B-hepatitis and compare it with those without histopathological changes. METHODS:We included 12 tissue samples showing chronic viral B hepatitis in the different stages of fibrosis and 7 tissue samples showing no histopathological changes. Immunohistochemical staining was performed using the streptavidin-biotin method. RESULTS:There was a regular presence of alpha-SMA immunoreactivity in tissue sections without histopathological changes in the portal tracts and also in liver parenchyma, while GFAP expression was noted in the periportal cavity. Tissue sections with the signs of chronic viral B hepatitis displayed very strong alpha-SMA expression in the portal tracts. A statistical analysis showed a positive correlation between the degree of liver fibrosis and alpha-SMA expression along the fibrous septa, whereas a negative correlation between the degree of liver fibrosis and alpha-SMA expression was present in the portal zone. CONCLUSION:This study showed the existance of two different stellate cell subpopulations in liver tissue. Differentiation between them was possible on the basis of SMA/GFAP expression.

journal_name

Vojnosanit Pregl

journal_title

Vojnosanitetski pregled

authors

Tomanović N,Boricić I,Brasanac D

doi

10.2298/vsp0606553t

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

553-7

issue

6

eissn

0042-8450

issn

2406-0720

journal_volume

63

pub_type

杂志文章
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    authors: Jovanović D,Kovacević Z,Rabrenović V,Skatarić V

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  • [Cytochemical and immunocytochemical characteristics of Meckel's diverticulum with heterotopic rests of pancreatic tissue].

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  • [Brain tumors in patients primarly treated psychiatrically].

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  • [Rational for the use of human immunoglobulins in clinical practice].

    abstract::The Clinic for Rheumatology and Clinical Immunology of the Military Medical Academy, with the participation of the hosts and experts from eminent Yugoslav institutions, in April 1997 organized the symposium on two current and trendy subjects from clinical immunology: "Antiphospholipid syndrome" and "The use of polyval...

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    authors: Popović M,Petronijević M,Stefanović D,Dimitrijević M

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  • [Lateral humeral epicondylitis].

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    authors: Ivanovski A

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    abstract:BACKGROUND/AIM:Obesity is the most common disease of nutrition and is the consequence of reduced movement. Unfortunately, this problem is increasingly present in juvenile age, so that the pediatric outpatient offices are dominated by obese young people. The aim of this study was to evaluate and quantify the effects of ...

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    doi:10.2298/vsp1201009s

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  • [Cerebellar hemangioblastomas--a study of the immunoprofile of neoplastic stromal component].

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