Abstract：
BACKGROUND/AIM:Analysis of organophosphosphorus compounds and their metabolites in a biological material includes the use of numerous methods, covering both preparation of samples for analysis and their identification that is considered to be very complex. Low concentrations monitoring requires implementation of highly sensitive analytical techniques. The aim of this study was to develop and validate an original and sensitive method for the detection and quantitation of organophosphorus pesticides (dimethoate, diazinon, malathion and malaoxon) in human biological matrices (serum, urine). METHODS:This method was based on a solid-phase extraction procedure, a chromatographic separation using an ACQUITY UPLC HSST3 column and mass spectrometric detection in the positive ion mode. Mobile phase: was consited of Solvent A (5 mM ammonium formate pH 3.0) and Solvent B (0.1% acetic formate in methanol), in a linear gradient (constant flow-rate 0.3 mL/min). RESULTS:The standard curve was linear in the range of 0.05-5.00 mg/L for malathion and malaoxon, 0.10-5.00 mg/L for dimethoate and 0.05-2.50 mg/L for diazinon. The correlation coefficient was r > or = 0.99. Extraction recoveries were satisfactory and ranged between 90-99%. The limits of detection (LOD) was between 0.007-0.07 mg/L and the limits of quantitation (LOQ) ranged between 0.022-0.085 mg/L. Intra- and interassay precision and accuracy were satisfactory for all of the pesticides analyzed. CONCLUSION:The method of liquid chromatography-mass spectrometry is simple, accurate, and useful for the determination of organophosphorus pesticides in both clinical and forensic toxicology.
journal_name
Vojnosanit Pregljournal_title
Vojnosanitetski pregledauthors
Zlatković M,Jovanović M,Djordjević D,Vucinić Sdoi
10.2298/vsp1009717zsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
717-22issue
9eissn
0042-8450issn
2406-0720journal_volume
67pub_type
杂志文章abstract：:Occurrence of methicillin resistant staphylococcal strains in hospital environment is more frequently found. These strains are multiresistant so that diseases caused by them require a special therapeutical approach. They are significant causative agents of intrahospital infections which may have epidemic character. Us...
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