Abstract:
UNLABELLED:125I-insulin binding and receptor-mediated insulin degradation were studied in isolated human fat cells from subcutaneous tissue. A high albumin concentration during cell isolation and incubation protected the fragile human adipocyte from lysis. Binding of tracer was pH dependent with an optimum between 7.4 and 7.6. At 37 degrees C steady state was reached by 45 min and maintained for at least 2 h. The binding of labelled insulin in the presence of 10 mumol/l unlabelled insulin was only 1-4% of the total insulin binding. The half-maximal displacement of tracer iodoinsulin (10 pmol/l) by unlabelled insulin occurred at 0.25 nmol/l. Kinetic studies of the dissociation of labelled iodoinsulin from fat cells showed a slight acceleration in the presence of a high concentration of unlabelled insulin in the washout buffer as compared to a buffer containing no insulin. At steady state binding about 95% of the cell-associated radioactivity was extracted as iodoinsulin as judged by gel filtration. The remaining 5% co-eluted with iodotyrosine. During 60 min about 90% of the cell-associated radioactivity dissociated as iodoinsulin and the rest as iodotyrosine. CONCLUSIONS:1) A high albumin content of buffers prevents traumatization of the human adipocyte; 2) under these conditions steady state binding of insulin is readily measured at 37 degrees C; 3) the use of a washing procedure makes the non-specific binding negligible; 4) the human adipocyte insulin receptor has a very high affinity; 5) receptor-mediated insulin degradation is minimal.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Pedersen O,Hjøllund E,Beck-Nielsen H,Lindskov HO,Sonne O,Gliemann Jsubject
Has Abstractpub_date
1981-06-01 00:00:00pages
636-41issue
6eissn
0012-186Xissn
1432-0428journal_volume
20pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract::During the period 1989-1994, 462 cases of insulin-dependent diabetes mellitus were registered among children from Northern Ireland aged under 15 years. The estimated completeness of the register was 98.8% (95% confidence interval (CI) 97.7%, 99.9%). A standardised rate of 19.6 (95% CI 17.8, 21.4) per 100 000 person ye...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400655
更新日期:1996-09-01 00:00:00
abstract::Using an antiserum raised to a recombinant coxsackie virus B 3 capsid protein, VP1, an immunocytochemical technique was developed which was capable of detecting the presence of all coxsackie B viruses in formalin fixed paraffin embedded infected tissue culture cells. This technique was tested on autopsy heart and panc...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00403323
更新日期:1990-05-01 00:00:00
abstract::Insulin antibodies expressed as insulin binding capacity of IgG were determined in 50 Type 1 (insulin-dependent) diabetic children who have been treated with monocomponent porcine insulin from the onset of the disease. During the follow-up period of 0.5-5.5 years (mean +/- SD: 3.2 +/- 1.6 years), 26 out of 50 patients...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00281121
更新日期:1984-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Previous studies show that inflammatory cytokines play a part in the development of insulin resistance. Thiazolidinediones were developed as insulin-sensitizing drugs and are ligands for the peroxisome proliferator-activated receptory (PPARgamma). We hypothesized that the anti-diabetic mechanism of thia...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051218
更新日期:1999-06-01 00:00:00
abstract::The development of diabetic glomerulopathy in kidneys transplanted to diabetic patients was estimated in transplant biopsies and evaluated in relation to suspected clinical risk factors for diabetic nephropathy. Surgical biopsies were taken at baseline and at 24-36 months post-transplantation in 16 Type 1 (insulin-dep...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400997
更新日期:1991-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Oxidized LDL is immunogenic and immune complexes formed by oxidized LDL and corresponding antibodies are pro-atherogenic and pro-inflammatory. Considering that macroalbuminuria is a risk factor for coronary heart disease and that common pathogenic factors for atherosclerosis and glomerulosclerosis exist...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-002-0962-y
更新日期:2002-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:Gestational diabetes mellitus (GDM) is a risk factor for perinatal complications. In several countries, the criteria for the diagnosis of GDM have been in flux, the American Diabetes Association (ADA) thresholds recommended in 2000 being lower than those of the National Diabetes Data Group (NDDG) that h...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0517-8
更新日期:2007-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:As glycaemia and the incidence of microvascular diabetes complications follow a log-linear relationship, it becomes increasingly difficult to demonstrate a microvascular benefit of glucose-lowering when the HbA1c level is close to normal. METHODS:The Outcome Reduction with an Initial Glargine Intervent...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-014-3238-4
更新日期:2014-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:An isoform of glutamic acid decarboxylase, (GAD)65 has been identified as a pancreatic beta-cell autoantigen in Type I (insulin dependent) diabetes mellitus. We investigated the expression of GAD isoforms among single rat beta cells in culture, under different conditions and the correlation between GAD6...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051275
更新日期:1999-09-01 00:00:00
abstract::The increase in urinary albumin excretion induced by acute exercise in Type 1 (insulin-dependent) diabetic patients is associated with the urinary excretion of cationic proteins. To test whether the renal excretion of glycated albumin (more anionic than non-glycated albumin) is affected by exercise, we submitted seven...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00408356
更新日期:1991-11-01 00:00:00
abstract::Demographic and biological data were collected from all Caucasian Type 1 diabetic patients (n = 279) who were recruited at clinical onset by the Belgian Diabetes Registry over 34 months. The male/female ratio was significantly higher for onset between age 20 and 40 years (2.4) than before age 20 years (1.0); no age-or...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00401060
更新日期:1993-11-01 00:00:00
abstract::At St. Mary's Hospital Medical School in London, 22 outpatients, 21 women and 1 man, underwent oral glucose tolerance tests. Carbohydrate intake was standardized for 3 days prior to each test. 13 of the women were on oral contraceptive therapy of the combination type. Tests were performed either at 9 AM after a fas...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01219788
更新日期:1973-06-01 00:00:00
abstract::Non-diabetic first degree relatives of non-insulin-dependent diabetic (NIDDM) families are at increased risk of developing diabetes mellitus, and have been studied to identify early metabolic abnormalities. Hormone concentrations measured by specific enzyme immunoassays were assessed in non-diabetic relatives of North...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050805
更新日期:1997-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Insulin control of glucose output is a major mechanism by which appropriate amounts of glucose are produced to supply energy to the central nervous system, without causing long-term increases of the plasma glucose concentration. It is hypothesised that the primary route by which insulin maintains contro...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s001250051474
更新日期:2000-07-01 00:00:00
abstract::Insulin resistance is a significant pathogenetic factor in the development of non-insulin-dependent diabetes mellitus (NIDDM). A new class of drugs, the thiazolidinediones, have been shown to lower blood glucose levels without stimulating insulin secretion. We report the metabolic effect of the thiazolidinedione, darg...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/BF00401763
更新日期:1995-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:Bariatric surgery consistently induces remission of type 2 diabetes. We tested whether there are diabetes-specific mechanisms in addition to weight loss. METHODS:We studied 25 morbidly obese patients (BMI 51.7 ± 1.5 kg/m(2) [mean ± SEM]), 13 with non-insulin-treated type 2 diabetes (HbA(1c) 7.1 ± 0.5% ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2193-6
更新日期:2011-08-01 00:00:00
abstract::The insulin-like growth factor I receptor and the activity of its associated tyrosine kinase activity were characterised in wheat germ agglutinin extracts from skeletal muscle biopsies from nine control and ten obese Type 2 (non-insulin-dependent) diabetic subjects, who had marked peripheral in vivo insulin resistance...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00265369
更新日期:1988-12-01 00:00:00
abstract::The incorporation of [35S]sulfate into heart proteoglycans has been studied in normal and alloxan-diabetic rats by perfusion and in vivo administration of the isotope; in the latter situation, comparison was also made of radiolabeled sulfate utilization by several other tissues (kidney, liver, lung, muscle, testes and...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00270425
更新日期:1987-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Hyperproinsulinaemia reflects both beta cell dysfunction and insulin resistance in cross-sectional studies, but it is not known whether changes in proinsulin concentrations are related to insulin resistance over time. As trans10cis12 (t10c12)-conjugated linoleic acid (CLA) supplementation induces insuli...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00125-004-1421-8
更新日期:2004-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:To investigate whether proinsulin C peptide influences sympathetic nerve activity directly or indirectly through parasympathetic nerve activity. METHODS:The proliferative response of splenic lymphocytes to Concanavalin A (ConA response) which is known to be suppressed by subjection of rats to footshock...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051562
更新日期:2000-12-01 00:00:00
abstract::The relation between urinary albumin excretion rate (UAE), transcapillary escape rate of albumin (TERalb), haemostatic factors, ambulatory blood pressure, and metabolic variables was investigated in 45 Type II (non-insulin-dependent) diabetic patients without overt nephropathy or uncontrolled blood pressure. We enroll...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/s001250051114
更新日期:1999-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Most type 2 diabetes-associated genetic variants identified via genome-wide association studies (GWASs) appear to act via the pancreatic islet. Observed defects in insulin secretion could result from an impact of these variants on islet development and/or the function of mature islets. Most functional s...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-018-4612-4
更新日期:2018-07-01 00:00:00
abstract::A recent investigation of families containing two and three consecutive generations affected with Type I (insulin dependent) diabetes mellitus has led to speculation that there is a second susceptibility gene, not linked to the major histocompatibility complex. These families differ in important respects from families...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00252759
更新日期:1981-05-01 00:00:00
abstract::The involvement of nitric oxide as an intracellular messenger in the control of insulin secretion from pancreatic Beta cells was studied in rat islets of Langerhans by measuring: (i) nitric oxide generation in response to physiological insulin secretagogues; (ii) the effects of inhibitors of nitric oxide synthesis on ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF02221676
更新日期:1992-11-01 00:00:00
abstract::Present-day treatments for people that are insulin dependent require multiple insulin injections, sometimes with an insulin pump, coupled with regular blood glucose monitoring. The availability of modified insulins, each with peaks of activity at varying times, has improved diabetes management. On the other hand, ther...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-020-05367-2
更新日期:2021-01-16 00:00:00
abstract:AIMS/HYPOTHESIS:Adipose tissue expresses some bioactive molecules, which may be involved in the development of obesity-associated metabolic disorders and cardiovascular diseases. Vascular endothelial growth factor (VEGF) an important angiogenic factor is implicated in normal and pathological vessel formation. The aim o...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-003-1221-6
更新日期:2003-11-01 00:00:00
abstract::Subjects selected from a centre specializing in diabetes detection have been classified using nine different methods of diagnosis including the recent criteria of the National Diabetes Data Group and the World Health Organization. The applicability of these two new criteria have been evaluated and compared with seven ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00297390
更新日期:1983-02-01 00:00:00
abstract::The Neurometer is a variable constant current sine wave stimulator, and has recently been proposed as a simple non-invasive and quantitative measure of peripheral nerve function. The device is portable and battery operated; assessment of upper and lower extremities takes only a few min, in contrast to conventional ass...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00274531
更新日期:1989-10-01 00:00:00
abstract::The oscillatory potential of the electroretinogram and the initial 2-min phase of dark-adaptation were studied in seven newly diagnosed Type 1 (insulin-dependent) diabetic patients before and during initial insulin treatment. Strict metabolic control was achieved in all seven patients using multiple subcutaneous injec...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00250163
更新日期:1983-03-01 00:00:00
abstract::Regeneration of neonatal beta cells after streptozotocin (STZ)-induced destruction may be due to either replication from pre-existing intra-islet beta cells or extra-islet precursor cells. To further investigate this issue, beta-cell growth was analysed in normal and streptozotocin-treated newborn rats (100 micrograms...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00418372
更新日期:1994-11-01 00:00:00