Abstract：
:A microplate radioimmunoassay for IgG, IgA and IgM antiglobulins reactive with rabbit IgG was performed on sera from 69 patients with various connective tissue diseases. IgM antiglobulins were detected in rheumatoid arthritis, systemic sicca syndrome and some patients with systemic vasculitis. IgA antiglobulins were found in seven of 10 patients with systemic sicca syndrome and only five of 59 patients with other connective tissue diseases. There was no correlation between the levels of IgM and IgA antiglobulins in the systemic sicca syndrome or rheumatoid arthritis (r = -0.21 and 0.2 respectively). IgG antiglobulins were not detected in any serum which lacked IgM or IgA antiglobulins. IgG isolated by DEAE columns showed antiglobulin activity in six of 15 rheumatoid arthritis and two of 10 systemic sicca syndrome sera tested. Antiglobulin analysis of sera fractionated by sucrose density ultracentrifugation at neutral and acid pH enabled the size of each class of antiglobulin to be determined. In certain sera, antiglobulin activity extended into the denser region of the gradient at pH 7.2 suggesting that the antiglobulins were complexed.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Elkon KB,Caeiro F,Gharavi AE,Patel BM,Ferjencik PP,Hughes GRsubject
Has Abstractpub_date
1981-12-01 00:00:00pages
547-56issue
3eissn
0009-9104issn
1365-2249journal_volume
46pub_type
杂志文章abstract：:The capacity of peripheral blood mononuclear (PBM) cells from patients with leprosy to generate immunoglobulin-secreting cells in response to pokeweed mitogen (PWM) was evaluated by a reverse haemolytic plaque forming cell (PFC) assay. The PFC responses of PBM cells from patients with lepromatous (Lpr) leprosy were si...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:Human thyroid epithelial cells (thyrocytes) express HLA Class II molecules in autoimmune thyroid diseases (ATD). Normal thyrocytes do not express Class II, but can be induced to do so by culture with interferon-gamma (gamma-IFN). We have examined HLA-D subregion expression in sections and monolayers of thyroid by indi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:Frozen sections of control and coeliac jejunal mucosa were examined for HLA-DR staining. In general the staining pattern in the controls and treated coeliac patients was a patchy subapical pattern on villous tips. Untreated coeliac patients gave a different pattern with staining extending to the crypt enterocytes. Glu...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12943
更新日期：2017-06-01 00:00:00
abstract：:Using the natural killer (NK) sensitive K562 cell line, enhanced NK cell cytotoxicity was demonstrable early in the course of acute hepatitis B while normal values were obtained in patients studied during convalescence. No evidence of enhanced NK activity was instead obtained in the course of acute non-A, non-B hepati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:The effects of 2.5-40 mg/kg nandrolone decanoate (ND) on the development and growth of mononuclear cell infiltrations in the submandibular glands of NZB/W (B/W) mice have been studied using a quantitative histological method. Injections with ND for 9 months, once every 3 weeks, starting at 4 weeks of age, reduced the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Binding of lymphocytes to human umbilical vein endothelial cells (EC) was quantitated by measuring adhesion of 51Cr labelled lymphocytes to endothelial cell monolayers and rosette formation between lymphocytes and EC in suspension. Mitogen stimulated human peripheral blood mononuclear cell culture supernatants and mix...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:The HLA phenotype of fifty-four patients with myasthenia gravis (MG) was compared to that of 600 controls. When male and female patients were compared separately with the control group, HLA-B12 was increased in MG males (P less than 0-023) and HLA-A1, B8 in MG females (P less than 0-023). In addition, HLA-A1, B8 was c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Superantigens (SAgs) are potent stimulators of T cells bearing specific Vbeta T cell receptors (TCR) and may play a role in the pathogenesis of Kawasaki syndrome (KS), although despite 15 years of intense study this area remains controversial. Because SAgs can cause Vbeta restricted T cell activation in the absence of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,多中心研究
doi：10.1111/j.1365-2249.2007.03567.x
更新日期：2008-02-01 00:00:00
abstract：:The effect of anti-neutrophil cytoplasm autoantibodies (ANCA) on neutrophil activation was studied by pre-incubating neutrophils with IgG and F(ab')2 prepared from ANCA patients with Wegener's granulomatosis or microscopic polyarteritis. We measured the generation of inositol triphosphate (IP3) (a product of hydrolysi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05738.x
更新日期：1991-09-01 00:00:00
abstract：:Both antigen specific and non-specific immunoregulation by cells have been described in jirds infected with Brugia pahangi, but the contribution of serum factors to immunoregulatory phenomena in this infection has not been examined. The present study determined the effect of serum from normal or B. pahangi infected ji...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:Coeliac disease (CD) is described as an autoimmune enteropathy associated with the presence of IgG and IgA antigliadin and antitransglutaminase autoantibodies. While of diagnostic significance, the role of these autoantibodies in the immunopathogenesis of CD is elucidated. An inappropriate T cell immune response to gl...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02220.x
更新日期：2003-09-01 00:00:00
abstract：:Inspired by the advances presented at the Inflammation and Immune Resolution Plenary Session at the British Society for Immunology Congress, December 2017, in this issue of Clinical & Experimental Immunology we present a Review Series on Inflammation and Immune Resolution. Our selection ranges from an overview of curr...
journal_title：Clinical and experimental immunology
pub_type： 社论
doi：10.1111/cei.13155
更新日期：2018-07-01 00:00:00
abstract：:Eosinophils from the peritoneal washings of normal rats adhered to live or formalin-fixed schistosomula of Schistosoma mansoni in vitro, in the presence of heat-inactivated serum from infected rats. Eosinophil adherence caused permeability changes in the schistosomula as revealed by 51Cr release and methylene blue upt...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：:Reconstitution of Semliki forest virus infected nude mice with spleen cells from their immunocompetent nu/+ litter mates resulted in an abolition of the otherwise persistent brain virus, production of anti-SFV IgG, and development of normally absent brain pathology. The brain pathological changes, including demyelinat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：:In patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy (uSpA), synovial fluid mononuclear cells (SFMC) show proliferation to bacterial antigens that trigger ReA, i.e. Chlamydia, Yersinia, Campylobactor, Shigella and Salmonella species. We have shown previously that SFMC proliferate significantl...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03362.x
更新日期：2007-06-01 00:00:00
abstract：:A method is described which can be used to quantitate class II MHC antigens (HLA-DR) expressed by cells within tissue sections. A mouse anti-human HLA-DR monoclonal antibody is directly conjugated to the fungal enzyme glucose oxidase. The enzyme, in the presence of its substrate can be used to reduce tetrazolium salts...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Opisthorchis viverrini infection is associated with human hepatobiliary disease and cholangiocarcinoma, but the role of the immune response in the pathogenesis of infection is unclear. Here ultrasonography was used to examine the biliary tracts of residents from an endemic community. Delayed-type hypersensitivity resp...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb08151.x
更新日期：1991-05-01 00:00:00
abstract：:Perforin, one of the cytotoxic proteins of the immune system, plays a prominent role in protection against viral and bacterial infections. We investigated its expression in PBL and their CD3+, CD4+, CD8+ and CD16+ and/or CD56+ subpopulations in endurance athletes before and after a triathlon. Lymphocyte subpopulations...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01220.x
更新日期：2000-06-01 00:00:00
abstract：:To determine the role of chemokines in the pathogenesis of systemic sclerosis (SSc), we examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution in the affected skin, of MCP-1, MIP-1alpha and MIP-1beta in SSc patients. Serum levels of these chemokines we...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00929.x
更新日期：1999-07-01 00:00:00
abstract：:Previous studies have shown that rabbit anti-mouse immunoglobulin sera (anti-Ig) which kill non-activated B lymphocytes in the presence of complement, are incapable of doing so when the cells are activated by antigen or mitogen into mitosis. Results reported here indicate that the resistance is not dependent on either...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:Donor-specific CTL present within the cardiac allograft during a rejection episode are distinct from those that populate the cardiac allograft in the absence of rejection. Whereas the former generally have a high avidity for donor cells, the latter mainly have a low avidity for donor cells. This observation made us re...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.4441458.x
更新日期：1997-12-01 00:00:00
abstract：:In stimulated cultures, a proportion of human T lymphocytes form multilayer rosettes with sheep red blood cells (SRBC) and they can be easily separated from common E rosettes by velocity sedimentation. We have developed a simplified method for harvesting multilayer rosettes and quantifying them. Practical applications...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:L-selectin in one of the key members of the selectin family of adhesion molecules and initiates leucocyte attachment to specialized high endothelial venules. The shed form, which retains functional activity, can be detected in biological fluids and is increased in diseases of many kinds. In the present study, we inves...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.37744.x
更新日期：1996-05-01 00:00:00
abstract：:Factors involved in the production of autoimmune thyroiditis in thymectomized and sublethally irradiated rats were investigated. The study suggested that a gene linked to RT1 of the rat major histocompatibility complex (MHC), a selective depletion of suppressor T cells and high radiosensitivity of the thyroid gland we...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The aim of this study was to determine the contribution of neutrophils to adjuvant arthritis (AA) by in vivo depletion of peripheral blood neutrophils. Specific anti-neutrophil MoAb, RP3 (10 mg), or a control antibody was given twice daily on days 8-11 after injection of Mycobacterium tuberculosis in inbred male Sprag...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1997.263-ce1154.x
更新日期：1997-02-01 00:00:00
abstract：:The effect of administration of 0-2 mg of epinephrine (Parke-Davis) on circulatory lymphocytes was investigated in fifteen normal subjects. Blood samples were taken prior to, 10 and 20 min after, subcutaneous injections. Thymus-derived (T) cells were assayed by sheep red blood cell rosettes, bone marrow-derived (B) ce...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:The effects of surgery on the immune response (as measured by a variety of parameters) were examined in patients undergoing transurethral resection of the prostate (TURP) for benign disease. Two groups were assessed, one having the procedure performed under a general anaesthetic and the other under a spinal anaestheti...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:Twenty-seven patients with rheumatoid arthritis (RA) maintained on drug regimens were studied monthly for 6-10 months. Disease activity was assessed and levels of anti-RA associated nuclear antigen (RANA) and immune complexes were determined. Anti-RANA generally paralleled disease activity in 64% of cases. Immune comp...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:Clustering activity for neutrophil granulocytes was generated in pooled normal human serum (NHS) by incubation of the serum with preformed IgG aggregates, but not in heat-treated NHS (56 degrees C, 30 min), indicating that the function was complement-dependent. Judging from results of experiments with complement-defic...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb07972.x
更新日期：1993-08-01 00:00:00
