Abstract:
:LPS induces dendritic cell (DC) activation, but the precise in vivo mechanism is unclear since DCs express low levels of TLR4. Here, it is shown that DCs can be activated in response to LPS through a bystander mechanism. This result was obtained using chimeric mice reconstituted with LPS-responsive and nonresponsive bone marrow cells. Thus, after indirect in vivo conditioning by LPS, bystander-activated DCs (LPS nonresponsive) up-regulated CD86. This up-regulation occurred even when LPS-responsive cells were MyD88 deficient. Functional analysis demonstrated that in vivo LPS conditioning endowed both the LPS-responsive and bystander cells with the ability to produce IFN-gamma in response to TLR9 stimulation in vitro. IFN-gamma production was also shown to be important for enhanced T-bet gene expression but not important for up-regulation of CD86. To investigate aspects of the mechanism, we used intracellular cytokine staining and found that NKDCs were responsible for at least some of the IFN-gamma production. Thus, our in vivo results demonstrated that bacterial LPS can bridge activation of various cellular populations of the innate immune system through a bystander mechanism.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Yadav R,Zammit DJ,Lefrancois L,Vella ATdoi
10.1189/jlb.0406253subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1251-61issue
6eissn
0741-5400issn
1938-3673pii
jlb.0406253journal_volume
80pub_type
杂志文章abstract::Inbred strains of mice, notably the susceptible C57BL/6 and the resistant A/J strains of mice, were infected with a strain of Mycobacterium evium. The infection in the visceral organs of mice was then studied, and the effect of colony-stimulating factors, i.e., interleukin-3 (IL-3), granulocyte-macrophage colony-stimu...
journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1011510
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journal_title:Journal of leukocyte biology
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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