Dissociation between the translocation and the activation of Akt in fMLP-stimulated human neutrophils--effect of prostaglandin E2.

Abstract:

:PGE(2) and other cAMP-elevating agents are known to down-regulate most functions stimulated by fMLP in human polymorphonuclear neutrophils. We reported previously that the inhibitory potential of PGE(2) resides in its capacity to suppress fMLP-stimulated PI-3Kgamma activation via the PGE(2) receptor EP(2) and hence, to decrease phosphatidylinositol 3,4,5-triphosphate [PI(3,4,5)P(3)] formation. Akt activity is stimulated by fMLP through phosphorylation on threonine 308 (Thr308) and serine 473 (Ser473) by 3-phosphoinositide-dependent kinase 1 (PDK1) and MAPK-AP kinase (APK)-APK-2 (MAPKAPK-2), respectively, in a PI-3K-dependent manner. Despite the suppression of fMLP-induced PI-3Kgamma activation observed in the presence of PGE(2), we show that Akt is fully phosphorylated on Thr308 and Ser473. However, fMLP-induced Akt translocation is decreased markedly in this context. PGE(2) does not affect the phosphorylation of MAPKAPK-2 but decreases the translocation of PDK1 induced by fMLP. Other cAMP-elevating agents such as adenosine (Ado) similarly block the fMLP-induced PI-3Kgamma activation process but do not inhibit Akt phosphorylation. However, Akt activity stimulated by fMLP is down-regulated slightly by agonists that elevate cAMP levels. Whereas protein kinase A is not involved in the maintenance of Akt phosphorylation, it is required for the inhibition of Akt translocation by PGE(2). Moreover, inhibition of fMLP-stimulated PI-3Kdelta activity by the selective inhibitor IC87114 only partially affects the late phase of Akt phosphorylation in the presence of PGE(2). Taken together, these results suggest that cAMP-elevating agents, such as PGE(2) or Ado, are able to induce an alternative mechanism of Akt activation by fMLP in which the translocation of Akt to PI(3,4,5)P(3)-enriched membranes is not required prior to its phosphorylation.

journal_name

J Leukoc Biol

authors

Burelout C,Naccache PH,Bourgoin SG

doi

10.1189/jlb.0406256

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

1523-34

issue

6

eissn

0741-5400

issn

1938-3673

pii

jlb.0406256

journal_volume

81

pub_type

杂志文章
  • Autocrine IL-10 partially prevents differentiation of neonatal dendritic epidermal leukocytes into Langerhans cells.

    abstract::To test whether reduced immune responsiveness in early life may be related to the immaturity of neonatal antigen-presenting cells, we comparatively assessed the phenotypic and functional characteristics of dendritic epidermal leukocytes (DEL) and epidermal Langerhans cells (LC) in newborn (NB) and adult mice, respecti...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0204087

    authors: Chang-Rodriguez S,Ecker R,Stingl G,Elbe-Bürger A

    更新日期:2004-09-01 00:00:00

  • HLA-A2 down-regulation on primary human macrophages infected with an M-tropic EGFP-tagged HIV-1 reporter virus.

    abstract::Multiple mechanisms are used by the human immunodeficiency virus type 1 (HIV-1) to interfere with host-cell immune effector functions. The 27-kD Nef protein has been shown to down-modulate specific genes of the major histocompatibility complex class I (MHC-I) on the surface of infected primary T cells, facilitating th...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0505237

    authors: Brown A,Gartner S,Kawano T,Benoit N,Cheng-Mayer C

    更新日期:2005-09-01 00:00:00

  • Nerve growth factor regulates TNF-alpha production in mouse macrophages via MAP kinase activation.

    abstract::In this study, we examined the expression of nerve growth factor (NGF) and its receptors in mouse macrophages and the mechanisms involved in the effect of NGF on tumor necrosis factor (TNF)-alpha production. Macrophages expressed NGF and the NGF receptors TrkA and p75. Treatment of J744 cells or peritoneal macrophages...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Barouch R,Kazimirsky G,Appel E,Brodie C

    更新日期:2001-06-01 00:00:00

  • ICAM-1 mediates leukocyte-endothelium adhesive interactions in the reversed passive Arthus reaction.

    abstract::A murine anti-rat intercellular adhesion molecule 1 (ICAM-1) monoclonal antibody (mAb), 1A29, was used to investigate the importance of blood leukocyte-associated beta 2-integrin (CD11/CD18) vascular endothelium-associated ICAM-1 adhesive interactions in the reversed passive Arthus reaction (RPAR) in rats. An Arthus p...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.59.3.333

    authors: Smith RJ,Chosay JG,Dunn CJ,Manning AM,Justen JM

    更新日期:1996-03-01 00:00:00

  • Epitope mapping of Ly-49G and G-like receptors: CK-1 antibody defines a polymorphic site of functional interaction with class I ligand.

    abstract::Ly-49 receptors regulate mouse natural killer cell functions. Members of the polymorphic Ly-49 multigene family recognize specific alleles of major histocompatibility complex class I (MHC I) or MHC I-like proteins. Previous studies have provided insight into the nature of Ly-49A and -C interaction with their high-affi...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0704407

    authors: Osman MS,Silver ET,Varghese JC,Chang CS,Gong DE,Audette GF,Hazes B,Kane KP

    更新日期:2005-05-01 00:00:00

  • Macrophage-elicited osteoclastogenesis in response to Brucella abortus infection requires TLR2/MyD88-dependent TNF-α production.

    abstract::Osteoarticular complications are common in human brucellosis, but the pathogenic mechanisms involved are largely unknown. In this manuscript, we described an immune mechanism for inflammatory bone loss in response to infection by Brucella abortus. We established a requirement for MyD88 and TLR2 in TNF-α-elicited osteo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.04111185

    authors: Delpino MV,Barrionuevo P,Macedo GC,Oliveira SC,Genaro SD,Scian R,Miraglia MC,Fossati CA,Baldi PC,Giambartolomei GH

    更新日期:2012-02-01 00:00:00

  • Late IL-3-induced phenotypic and functional alterations in human basophils require continuous IL-3 receptor signaling.

    abstract::Cytokines of the GM-CSF family signal via the same receptor subunit (βc) and, thus, have overlapping effects on cells that express all cytokine-specific α-chains (IL-3Rα, IL-5Rα, GM-CSFRα), such as human basophils, whose rapid effector functions are similarly enhanced by IL-3, IL-5, and GM-CSF. However, previous work ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.2A0715-292RR

    authors: Kämpfer SS,Odermatt A,Dahinden CA,Fux M

    更新日期:2017-01-01 00:00:00

  • 20-Hydroxy- and 20-carboxy-leukotriene (LT) B4 downregulate LTB4 -mediated responses of human neutrophils and eosinophils.

    abstract::Leukotriene B4 (LTB4 ) plays a prominent role in innate immunity as it induces phagocyte recruitment, the release of antimicrobial effectors, and as it potentiates the ingestion and killing of pathogens. In humans, LTB4 has a short half-life and is rapidly metabolized by leukocytes, notably into 20-OH- and 20-COOH-LTB...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.MA0718-306R

    authors: Archambault AS,Poirier S,Lefebvre JS,Robichaud PP,Larose MC,Turcotte C,Martin C,Provost V,Boudreau LH,McDonald PP,Laviolette M,Surette ME,Flamand N

    更新日期:2019-06-01 00:00:00

  • Controlling the Toll road to dendritic cell polarization.

    abstract::The activation of dendritic cells (DC) via Toll-like receptors (TLRs) plays a decisive role in shaping the outcome of primary immune responses. Following TLR engagement by microbial products, DC migrate from peripheral tissues to lymphoid organs and up-regulate major histocompatibility complex and costimulatory molecu...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.1003482

    authors: Mazzoni A,Segal DM

    更新日期:2004-05-01 00:00:00

  • Unraveling exhaustion in adaptive and conventional NK cells.

    abstract::Immune exhaustion in T cells significantly impacts their ability to control malignancies and infections, and its discovery has led to revolutionary therapies for cancer in the form of checkpoint blockade. NK cells, like T cells, are lymphocytes that recognize virally infected and malignantly transformed cells. However...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/JLB.4MR0620-091R

    authors: Merino AM,Kim H,Miller JS,Cichocki F

    更新日期:2020-10-01 00:00:00

  • Senescence in innate immune responses: reduced neutrophil phagocytic capacity and CD16 expression in elderly humans.

    abstract::Elderly humans are more susceptible to bacterial infections because of declining immune status. We have investigated the effect of aging on neutrophil bactericidal responses, comparing neutrophil function in healthy, young (23-35 years) and elderly (>65 years) volunteers. Superoxide generation in response to fMLP was ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Butcher SK,Chahal H,Nayak L,Sinclair A,Henriquez NV,Sapey E,O'Mahony D,Lord JM

    更新日期:2001-12-01 00:00:00

  • Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages.

    abstract::The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/Mphi differentiation and cellular activation. We isolated clonal popula...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.64.1.85

    authors: Fiani ML,Beitz J,Turvy D,Blum JS,Stahl PD

    更新日期:1998-07-01 00:00:00

  • The inflammatory potential of IL-2: local induction of a specific chronic granulomatous lesion in mice.

    abstract::Subcutaneous injection of recombinant human interleukin-2 (rhuIL-2) at 10(2)-10(4) U/mouse induced delayed (48 h) accumulation of mononuclear leukocytes with diffuse granulocytes, including eosinophils. Subcutaneous local infusion of rhuIL-2 or recombinant murine IL-2 (10(2)-10(4) U/mouse) via implanted Alzet miniosmo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.60.1.27

    authors: Dunn CJ,Hardee MM,Fidler SF,Shields SK,Chosay JG

    更新日期:1996-07-01 00:00:00

  • Leukocyte-specific protein 1 links TNF receptor-associated factor 1 to survival signaling downstream of 4-1BB in T cells.

    abstract::4-1BB is a member of the TNFR superfamily, which contributes to the activation of signaling pathways required for the survival of activated and memory T cells. We have shown previously that TRAF1, an adaptor protein recruited to 4-1BB, is required for 4-1BB-mediated CD8 T cell survival in vivo. With the use of a prote...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1112579

    authors: Sabbagh L,Andreeva D,Laramée GD,Oussa NA,Lew D,Bisson N,Soumounou Y,Pawson T,Watts TH

    更新日期:2013-05-01 00:00:00

  • Processing and intracellular transport of cathepsin G and neutrophil elastase in the leukemic myeloid cell line U-937-modulation by brefeldin A, ammonium chloride, and monensin.

    abstract::The effects of brefeldin A, monensin, and the weak base NH4Cl on the biosynthesis and processing of cathepsin G and neutrophil elastase of myeloid cells were investigated. Monoblast-like U-937 cells were biosynthetically labeled with [35S]methionine, followed by subcellular fractionation, immunoprecipitation, and anal...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.55.1.50

    authors: Lindmark A,Gullberg U,Olsson I

    更新日期:1994-01-01 00:00:00

  • Induction of NOS in rat blood PMN in vivo and in vitro: modulation by tyrosine kinase and involvement in bactericidal activity.

    abstract::Intravenous administration of lipopolysaccharide (LPS) to rats increased the production of nitric oxide (NO) metabolites (NOx) by blood polymorphonuclear neutrophils (PMN) in vitro. Both dexamethasone and L-NMMA, added in vitro to neutrophil cultures, inhibited the production of NO. On the other hand, the production o...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.65.4.508

    authors: Fierro IM,Nascimento-DaSilva V,Arruda MA,Freitas MS,Plotkowski MC,Cunha FQ,Barja-Fidalgo C

    更新日期:1999-04-01 00:00:00

  • Isolation and confinement as a model for spaceflight immune changes.

    abstract::The immune system of astronauts is disturbed during and after spaceflight. This situation may result from stressors encountered during the flight (microgravity, radiation, psychological stress, or confinement) or from physiological changes (demineralization or fluid shift). Therefore, ground-based investigations shoul...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/jlb.54.3.209

    authors: Schmitt DA,Schaffar L

    更新日期:1993-09-01 00:00:00

  • p120 nucleolar-proliferating antigen is a direct target of G-CSF signaling during myeloid differentiation.

    abstract::Granulocyte-colony stimulating factor (G-CSF) is an essential cytokine, which contributes to proliferation and differentiation of granulocyte precursor cells in the bone marrow. Despite recent progress in understanding G-CSF signaling events, the mechanisms that underlie the distinct spectrum of biological functions a...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0205066

    authors: Khanna-Gupta A,Sun H,Zibello T,Lozovatsky L,Ghosh PK,Link DC,McLemore ML,Berliner N

    更新日期:2006-05-01 00:00:00

  • Mast cell histamine promotes the immunoregulatory activity of myeloid-derived suppressor cells.

    abstract::It has been shown recently that MCs are required for differential regulation of the immune response by granulocytic versus monocytic MDSCs. Granulocytic MDSCs promoted parasite clearance, whereas monocytic MDSCs enhanced tumor progression; both activities were abrogated in MC-deficient mice. Herein, we demonstrate tha...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.5A1213-644R

    authors: Martin RK,Saleem SJ,Folgosa L,Zellner HB,Damle SR,Nguyen GK,Ryan JJ,Bear HD,Irani AM,Conrad DH

    更新日期:2014-07-01 00:00:00

  • Proteasome-mediated regulation of interleukin-1beta turnover and export in human monocytes.

    abstract::Interleukin-1beta is a secreted protein that accumulates in the cytosol as an inactive precursor (pIL-1beta) before processing and release of biologically active protein. To understand the impact of this property on IL-1beta production, we examined the intracellular stability of pIL-1beta in lipopolysaccharide (LPS)-s...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Moors MA,Mizel SB

    更新日期:2000-07-01 00:00:00

  • Monomeric bovine IgG2 is a potent stimulus for bovine neutrophils.

    abstract::Bovine plasma contains factors that can stimulate bovine neutrophils. Bovine plasma at 1:1,000,000-1:1,000 dilution induced a dose-dependent superoxide production in bovine neutrophils. When bovine plasma was fractionated with a protein G column, only the IgG fraction contained induction activity. At similar concentra...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.58.2.203

    authors: Tao W,Corbett MJ,Pickett W

    更新日期:1995-08-01 00:00:00

  • The macrophage scavenger receptor CD163: endocytic properties of cytoplasmic tail variants.

    abstract::CD163 is the monocyte/macrophage-specific receptor for haptoglobin-hemoglobin (Hp-Hb) complexes. The cytoplasmic tail of human CD163 exists as a short tail variant and two long tail variants. Reverse transcriptase-polymerase chain reaction analysis indicated that all three CD163 variants are substantially expressed in...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1005602

    authors: Nielsen MJ,Madsen M,Møller HJ,Moestrup SK

    更新日期:2006-04-01 00:00:00

  • Characterization of the interaction between serum mannan-binding protein and nucleic acid ligands.

    abstract::Serum MBP, also known as MBL, is a C-type lectin that is known to be a soluble host defense factor involved in innate immunity. It has been well established that dying microbes and apoptotic cells release highly viscous DNA that induces inflammation and septic shock, and apoptotic cells display fragmented DNA on their...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1008674

    authors: Nakamura N,Nonaka M,Ma BY,Matsumoto S,Kawasaki N,Asano S,Kawasaki T

    更新日期:2009-09-01 00:00:00

  • Neutrophil accumulation and plasma leakage induced in vivo by neutrophil-activating peptide-1.

    abstract::Neutrophil accumulation and plasma leakage induced in rabbit skin by neutrophil-activating peptide-1 (NAP-1, a 72 amino acid peptide produced by monocytes and a variety of tissue cells), E. coli endotoxin, and interleukin-1 (IL-1) were compared. Neutrophil accumulation at sites injected with NAP-1 was intense, rapid, ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.48.2.129

    authors: Colditz IG,Zwahlen RD,Baggiolini M

    更新日期:1990-08-01 00:00:00

  • Cell surface localization and release of the candidate tumor suppressor Ecrg4 from polymorphonuclear cells and monocytes activate macrophages.

    abstract::We identified fresh human leukocytes as an abundant source of the candidate epithelial tumor suppressor gene, Ecrg4, an epigenetically regulated gene, which unlike other tumor suppressor genes, encodes an orphan-secreted, ligand-like protein. In human cell lines, Ecrg4 gene expression was low, Ecrg4 protein undetectab...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1011503

    authors: Baird A,Coimbra R,Dang X,Lopez N,Lee J,Krzyzaniak M,Winfield R,Potenza B,Eliceiri BP

    更新日期:2012-05-01 00:00:00

  • PKD regulates actin polymerization, neutrophil deformability, and transendothelial migration in response to fMLP and trauma.

    abstract::Neutrophils are important mediators of the innate immune defense and of the host response to a physical trauma. Because aberrant infiltration of injured sites by neutrophils was shown to cause adverse effects after trauma, we investigated how neutrophil infiltration could be modulated at the cellular level. Our data i...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.4A0617-251RR

    authors: Wille C,Eiseler T,Langenberger ST,Richter J,Mizuno K,Radermacher P,Knippschild U,Huber-Lang M,Seufferlein T,Paschke S

    更新日期:2018-09-01 00:00:00

  • Effects of endotoxin on expression of VLA integrins by human bronchoalveolar lavage macrophages.

    abstract::Endotoxin (lipopolysaccharide, LPS) is known to induce inflammatory responses, such as monocyte/macrophage adherence, migration, and accumulation. Recruitment and accumulation of macrophages during infection and inflammation are regulated by integrin-mediated cell-extracellular matrix interactions. In the present repo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.57.4.624

    authors: Kang YH,Lee CH,Brummel SE,Newball HH,Forrester J

    更新日期:1995-04-01 00:00:00

  • Synergism of NOD2 and NLRP3 activators promotes a unique transcriptional profile in murine dendritic cells.

    abstract::NLRs are cytoplasmic proteins that sense cellular stress and intracellular damage resulting from pathogen uptake. To date, the role of NLRs has been studied using combinations of NLR and TLR agonists, but the interplay between two different NLRs remains uncharacterized. In this study, we employed microarrays to invest...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1009652

    authors: Conforti-Andreoni C,Beretta O,Licandro G,Qian HL,Urbano M,Vitulli F,Ricciardi-Castagnoli P,Mortellaro A

    更新日期:2010-12-01 00:00:00

  • CIRP increases ICAM-1+ phenotype of neutrophils exhibiting elevated iNOS and NETs in sepsis.

    abstract::Sepsis represents uncontrolled inflammation due to an infection. Cold-inducible RNA-binding protein (CIRP) is a stress-induced damage-associated molecular pattern (DAMP). A subset of neutrophils expressing ICAM-1+ neutrophils was previously shown to produce high levels of reactive oxygen species. The role of CIRP for ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.3A0817-327RR

    authors: Ode Y,Aziz M,Wang P

    更新日期:2018-04-01 00:00:00

  • Frontline Science: Characterization and regulation of osteoclast precursors following chronic Porphyromonas gingivalis infection.

    abstract::Bone destruction in inflammatory osteolytic diseases including periodontitis is related to excessive activity of osteoclasts (OC), which originate from precursor cells of the myeloid lineage, termed osteoclast precursors (OCP). In contrast to ample knowledge that we currently have on mature OC, little is known about O...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.1HI0620-230R

    authors: Zhao Y,Li Z,Su L,Ballesteros-Tato A,Katz J,Michalek SM,Feng X,Zhang P

    更新日期:2020-10-01 00:00:00